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P11672

- NGAL_MOUSE

UniProt

P11672 - NGAL_MOUSE

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Protein

Neutrophil gelatinase-associated lipocalin

Gene

Lcn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281Catecholate-type ferric siderophoreBy similarity
Binding sitei147 – 1471Catecholate-type ferric siderophoreBy similarity
Binding sitei156 – 1561Catecholate-type ferric siderophoreBy similarity

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB
  2. small molecule binding Source: InterPro
  3. transporter activity Source: InterPro

GO - Biological processi

  1. cellular response to hydrogen peroxide Source: Ensembl
  2. cellular response to interleukin-1 Source: Ensembl
  3. cellular response to lipopolysaccharide Source: Ensembl
  4. cellular response to nutrient levels Source: Ensembl
  5. cellular response to tumor necrosis factor Source: Ensembl
  6. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  7. innate immune response Source: UniProtKB
  8. ion transport Source: UniProtKB-KW
  9. iron ion homeostasis Source: UniProtKB-KW
  10. positive regulation of cell projection organization Source: Ensembl
  11. positive regulation of gene expression Source: Ensembl
  12. protein homotrimerization Source: Ensembl
  13. response to drug Source: Ensembl
  14. response to herbicide Source: Ensembl
  15. response to virus Source: MGI
  16. siderophore transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Immunity, Innate immunity, Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil gelatinase-associated lipocalin
Short name:
NGAL
Alternative name(s):
Lipocalin-2
SV-40-induced 24P3 protein
Siderocalin LCN2
p25
Gene namesi
Name:Lcn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:96757. Lcn2.

Subcellular locationi

Secreted 2 Publications
Note: Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. extracellular region Source: UniProtKB
  3. extracellular space Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mice are normal with no visible phenotype. They however show an increased susceptibility to bacterial infections. Neutrophils show significantly less bacteriostatic activity.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 200180Neutrophil gelatinase-associated lipocalinPRO_0000017934Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211Pyrrolidone carboxylic acidBy similarity
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi85 – 851N-linked (GlcNAc...)1 Publication
Disulfide bondi98 ↔ 1971 Publication

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP11672.
PaxDbiP11672.
PRIDEiP11672.

PTM databases

PhosphoSiteiP11672.

Expressioni

Tissue specificityi

Detected in lung, spleen, uterus, vagina and epididymis.1 Publication

Inductioni

Upon Toll-like receptor (TLRs) stimuli. By SV-40.1 Publication

Gene expression databases

BgeeiP11672.
CleanExiMM_LCN2.
GenevestigatoriP11672.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heterodimer; disulfide-linked with MMP9.1 Publication

Structurei

Secondary structure

1
200
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni33 – 353
Turni44 – 474
Beta strandi49 – 5810
Turni63 – 653
Beta strandi73 – 786
Beta strandi84 – 918
Beta strandi98 – 10710
Beta strandi113 – 1175
Helixi119 – 1213
Beta strandi125 – 13511
Beta strandi137 – 14913
Beta strandi152 – 16413
Helixi168 – 18013
Helixi185 – 1873
Beta strandi188 – 1925
Beta strandi195 – 1973

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3S26X-ray1.80A21-200[»]
3U9PX-ray2.80C/D21-200[»]
ProteinModelPortaliP11672.
SMRiP11672. Positions 27-200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the calycin superfamily. Lipocalin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40148.
GeneTreeiENSGT00530000063610.
HOGENOMiHOG000231660.
HOVERGENiHBG106490.
InParanoidiP11672.
OMAiVPIDQCI.
OrthoDBiEOG78M03G.
PhylomeDBiP11672.
TreeFamiTF336103.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR003087. N_gelatinase.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00179. LIPOCALIN.
PR01275. NGELATINASE.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00213. LIPOCALIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11672-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALSVMCLGL ALLGVLQSQA QDSTQNLIPA PSLLTVPLQP DFRSDQFRGR
60 70 80 90 100
WYVVGLAGNA VQKKTEGSFT MYSTIYELQE NNSYNVTSIL VRDQDQGCRY
110 120 130 140 150
WIRTFVPSSR AGQFTLGNMH RYPQVQSYNV QVATTDYNQF AMVFFRKTSE
160 170 180 190 200
NKQYFKITLY GRTKELSPEL KERFTRFAKS LGLKDDNIIF SVPTDQCIDN
Length:200
Mass (Da):22,875
Last modified:October 1, 1989 - v1
Checksum:iDD9A8D08750E6863
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14607 mRNA. Translation: CAA32762.1.
X81627 Genomic DNA. Translation: CAA57283.1.
AK149774 mRNA. Translation: BAE29077.1.
AL808027 Genomic DNA. Translation: CAM15869.1.
CH466542 Genomic DNA. Translation: EDL08545.1.
BC132069 mRNA. Translation: AAI32070.1.
BC132071 mRNA. Translation: AAI32072.1.
S82469 mRNA. No translation available.
CCDSiCCDS15913.1.
PIRiS07397.
RefSeqiNP_032517.1. NM_008491.1.
UniGeneiMm.9537.

Genome annotation databases

EnsembliENSMUST00000050785; ENSMUSP00000053962; ENSMUSG00000026822.
GeneIDi16819.
KEGGimmu:16819.
UCSCiuc008jfl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14607 mRNA. Translation: CAA32762.1 .
X81627 Genomic DNA. Translation: CAA57283.1 .
AK149774 mRNA. Translation: BAE29077.1 .
AL808027 Genomic DNA. Translation: CAM15869.1 .
CH466542 Genomic DNA. Translation: EDL08545.1 .
BC132069 mRNA. Translation: AAI32070.1 .
BC132071 mRNA. Translation: AAI32072.1 .
S82469 mRNA. No translation available.
CCDSi CCDS15913.1.
PIRi S07397.
RefSeqi NP_032517.1. NM_008491.1.
UniGenei Mm.9537.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3S26 X-ray 1.80 A 21-200 [» ]
3U9P X-ray 2.80 C/D 21-200 [» ]
ProteinModelPortali P11672.
SMRi P11672. Positions 27-200.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P11672.

Proteomic databases

MaxQBi P11672.
PaxDbi P11672.
PRIDEi P11672.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050785 ; ENSMUSP00000053962 ; ENSMUSG00000026822 .
GeneIDi 16819.
KEGGi mmu:16819.
UCSCi uc008jfl.1. mouse.

Organism-specific databases

CTDi 3934.
MGIi MGI:96757. Lcn2.

Phylogenomic databases

eggNOGi NOG40148.
GeneTreei ENSGT00530000063610.
HOGENOMi HOG000231660.
HOVERGENi HBG106490.
InParanoidi P11672.
OMAi VPIDQCI.
OrthoDBi EOG78M03G.
PhylomeDBi P11672.
TreeFami TF336103.

Miscellaneous databases

ChiTaRSi LCN2. mouse.
NextBioi 290708.
PROi P11672.
SOURCEi Search...

Gene expression databases

Bgeei P11672.
CleanExi MM_LCN2.
Genevestigatori P11672.

Family and domain databases

Gene3Di 2.40.128.20. 1 hit.
InterProi IPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR022272. Lipocalin_CS.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR003087. N_gelatinase.
[Graphical view ]
Pfami PF00061. Lipocalin. 1 hit.
[Graphical view ]
PRINTSi PR00179. LIPOCALIN.
PR01275. NGELATINASE.
SUPFAMi SSF50814. SSF50814. 1 hit.
PROSITEi PS00213. LIPOCALIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism."
    Hraba-Renevey S., Turler H., Kress M., Salomon C., Weil R.
    Oncogene 4:601-608(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss.
    Tissue: Kidney.
  2. "An apparent autocrine mechanism amplifies the dexamethasone- and retinoic acid-induced expression of mouse lipocalin-encoding gene 24p3."
    Garay-Rojas E., Harper M., Hraba-Renevey S., Kress M.
    Gene 170:173-180(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  7. "Demonstration of a glycoprotein derived from the 24p3 gene in mouse uterine luminal fluid."
    Chu S.T., Huang H.L., Chen J.M., Chen Y.H.
    Biochem. J. 316:545-550(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-200, PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "The first lipocalin with enzymatic activity."
    Peitsch M.C., Boguski M.S.
    Trends Biochem. Sci. 16:363-363(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO THE LIPOCALIN FAMILY.
  9. "Mouse oncogene protein 24p3 is a member of the lipocalin protein family."
    Flower D.R., North A.C.T., Attwood T.K.
    Biochem. Biophys. Res. Commun. 180:69-74(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO THE LIPOCALIN FAMILY.
  10. Cited for: FUNCTION, IRON-BINDING, SIDEROPHORE-BINDING, SUBCELLULAR LOCATION.
  11. "Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron."
    Flo T.H., Smith K.D., Sato S., Rodriguez D.J., Holmes M.A., Strong R.K., Akira S., Aderem A.
    Nature 432:917-921(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IRON-BINDING, SIDEROPHORE-BINDING, DISRUPTION PHENOTYPE, INDUCTION.
  12. "A cell-surface receptor for lipocalin 24p3 selectively mediates apoptosis and iron uptake."
    Devireddy L.R., Gazin C., Zhu X., Green M.R.
    Cell 123:1293-1305(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SLC22A17, IRON-BINDING.
  13. "Lipocalin 2-deficient mice exhibit increased sensitivity to Escherichia coli infection but not to ischemia-reperfusion injury."
    Berger T., Togawa A., Duncan G.S., Elia A.J., You-Ten A., Wakeham A., Fong H.E., Cheung C.C., Mak T.W.
    Proc. Natl. Acad. Sci. U.S.A. 103:1834-1839(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  14. "A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production."
    Devireddy L.R., Hart D.O., Goetz D.H., Green M.R.
    Cell 141:1006-1017(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IRON-BINDING, SIDEROPHORE-BINDING.
  15. "Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors."
    Bandaranayake A.D., Correnti C., Ryu B.Y., Brault M., Strong R.K., Rawlings D.J.
    Nucleic Acids Res. 39:E143-E143(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 21-200, DISULFIDE BOND, GLYCOSYLATION AT ASN-85.

Entry informationi

Entry nameiNGAL_MOUSE
AccessioniPrimary (citable) accession number: P11672
Secondary accession number(s): Q3UE34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 29, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3