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Protein

Guanine nucleotide-exchange factor SEC12

Gene

SEC12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER. This function involves the cytoplasmic domain of the protein, which is thought to interact with the small GTP-binding protein SAR1. Required for autophagy.16 Publications

Miscellaneous

In the process of transport, SEC12 itself may migrate to the Golgi apparatus and function in subsequent transport events.
Present with 6160 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • protein secretion Source: GO_Central
  • regulation of COPII vesicle coating Source: SGD
  • vesicle-mediated transport Source: UniProtKB-KW

Keywordsi

Molecular functionGTPase activation
Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33339-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-exchange factor SEC12
Alternative name(s):
Protein transport protein SEC12
Gene namesi
Name:SEC12
Synonyms:SED2
Ordered Locus Names:YNR026C
ORF Names:N3244
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR026C
SGDiS000005309 SEC12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 354Cytoplasmic1 PublicationAdd BLAST354
Transmembranei355 – 373Helical; Signal-anchor for type II membrane proteinAdd BLAST19
Topological domaini374 – 471Lumenal1 PublicationAdd BLAST98

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000976571 – 471Guanine nucleotide-exchange factor SEC12Add BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi439N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi462N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP11655
PaxDbiP11655
PRIDEiP11655

Interactioni

GO - Molecular functioni

  • GTPase binding Source: GO_Central
  • Sar guanyl-nucleotide exchange factor activity Source: SGD

Protein-protein interaction databases

BioGridi35850, 709 interactors
DIPiDIP-7541N
IntActiP11655, 2 interactors
MINTiP11655
STRINGi4932.YNR026C

Structurei

Secondary structure

1471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi14 – 21Combined sources8
Beta strandi24 – 30Combined sources7
Beta strandi33 – 37Combined sources5
Beta strandi40 – 47Combined sources8
Beta strandi51 – 53Combined sources3
Helixi54 – 57Combined sources4
Beta strandi58 – 65Combined sources8
Beta strandi75 – 79Combined sources5
Beta strandi82 – 86Combined sources5
Helixi91 – 96Combined sources6
Beta strandi103 – 109Combined sources7
Turni110 – 113Combined sources4
Beta strandi114 – 121Combined sources8
Beta strandi133 – 138Combined sources6
Beta strandi145 – 150Combined sources6
Beta strandi155 – 160Combined sources6
Turni161 – 164Combined sources4
Beta strandi165 – 171Combined sources7
Beta strandi178 – 181Combined sources4
Beta strandi185 – 191Combined sources7
Beta strandi196 – 200Combined sources5
Turni201 – 203Combined sources3
Beta strandi206 – 210Combined sources5
Beta strandi217 – 226Combined sources10
Beta strandi229 – 239Combined sources11
Beta strandi242 – 250Combined sources9
Beta strandi253 – 265Combined sources13
Beta strandi269 – 274Combined sources6
Beta strandi280 – 285Combined sources6
Beta strandi290 – 294Combined sources5
Turni295 – 298Combined sources4
Beta strandi299 – 304Combined sources6
Beta strandi307 – 310Combined sources4
Beta strandi312 – 317Combined sources6
Beta strandi323 – 328Combined sources6
Beta strandi331 – 337Combined sources7
Turni340 – 343Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4H5IX-ray1.36A/B1-354[»]
4H5JX-ray2.60A/B1-354[»]
ProteinModelPortaliP11655
SMRiP11655
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati259 – 300WD 1Add BLAST42
Repeati302 – 341WD 2Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat SEC12 family.Curated

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

GeneTreeiENSGT00690000103233
HOGENOMiHOG000000861
InParanoidiP11655
KOiK14003
OMAiNKLRLYR
OrthoDBiEOG092C36UE

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
SMARTiView protein in SMART
SM00320 WD40, 2 hits

Sequencei

Sequence statusi: Complete.

P11655-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVTASYNV GYPAYGAKFL NNDTLLVAGG GGEGNNGIPN KLTVLRVDPT
60 70 80 90 100
KDTEKEQFHI LSEFALEDND DSPTAIDASK GIILVGCNEN STKITQGKGN
110 120 130 140 150
KHLRKFKYDK VNDQLEFLTS VDFDASTNAD DYTKLVYISR EGTVAAIASS
160 170 180 190 200
KVPAIMRIID PSDLTEKFEI ETRGEVKDLH FSTDGKVVAY ITGSSLEVIS
210 220 230 240 250
TVTGSCIARK TDFDKNWSLS KINFIADDTV LIAASLKKGK GIVLTKISIK
260 270 280 290 300
SGNTSVLRSK QVTNRFKGIT SMDVDMKGEL AVLASNDNSI ALVKLKDLSM
310 320 330 340 350
SKIFKQAHSF AITEVTISPD STYVASVSAA NTIHIIKLPL NYANYTSMKQ
360 370 380 390 400
KISKFFTNFI LIVLLSYILQ FSYKHNLHSM LFNYAKDNFL TKRDTISSPY
410 420 430 440 450
VVDEDLHQTT LFGNHGTKTS VPSVDSIKVH GVHETSSVNG TEVLCTESNI
460 470
INTGGAEFEI TNATFREIDD A
Length:471
Mass (Da):51,608
Last modified:October 1, 1989 - v1
Checksum:iDD4CEED041E776C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13161 Genomic DNA Translation: CAA31559.1
Z71641 Genomic DNA Translation: CAA96306.1
BK006947 Genomic DNA Translation: DAA10566.1
PIRiA31103
RefSeqiNP_014423.1, NM_001183203.1

Genome annotation databases

EnsemblFungiiYNR026C; YNR026C; YNR026C
GeneIDi855760
KEGGisce:YNR026C

Similar proteinsi

Entry informationi

Entry nameiSEC12_YEAST
AccessioniPrimary (citable) accession number: P11655
Secondary accession number(s): D6W1K0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 23, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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