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P11633

- NHP6B_YEAST

UniProt

P11633 - NHP6B_YEAST

Protein

Non-histone chromosomal protein 6B

Gene

NHP6B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6A.9 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi27 – 9569HMG boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding, bending Source: SGD
    2. nucleosome binding Source: SGD

    GO - Biological processi

    1. chromatin remodeling Source: SGD
    2. DNA repair Source: UniProtKB-KW
    3. maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase III promoter Source: SGD
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
    6. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    DNA damage, DNA repair, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29229-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-histone chromosomal protein 6B
    Gene namesi
    Name:NHP6B
    Synonyms:NHPB
    Ordered Locus Names:YBR089C-A
    ORF Names:YBR090BC
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR089c-a.
    SGDiS000002157. NHP6B.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation. Chromosome 1 Publication

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9999Non-histone chromosomal protein 6BPRO_0000048566Add
    BLAST

    Proteomic databases

    PaxDbiP11633.
    PeptideAtlasiP11633.

    Expressioni

    Gene expression databases

    GenevestigatoriP11633.

    Interactioni

    Subunit structurei

    Weakly associates with the stable SPT16-POB3 heterodimer to form the FACT (yFACT or SNP) complex, which is associated with nucleosomes. Multiple molecules of NHP6 (NHP6A and/or NHP6B) are required to recruit the SPT16-POB3 heterodimer to DNA.

    Protein-protein interaction databases

    BioGridi32795. 99 interactions.
    DIPiDIP-6748N.
    IntActiP11633. 23 interactions.
    MINTiMINT-615533.
    STRINGi4932.YBR089C-A.

    Structurei

    3D structure databases

    ProteinModelPortaliP11633.
    SMRiP11633. Positions 10-99.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NHP6 family.Curated
    Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5648.
    GeneTreeiENSGT00740000115458.
    HOGENOMiHOG000197861.
    OMAiLANPNNK.
    OrthoDBiEOG70KH25.

    Family and domain databases

    Gene3Di1.10.30.10. 1 hit.
    InterProiIPR009071. HMG_box_dom.
    [Graphical view]
    PfamiPF00505. HMG_box. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 1 hit.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 1 hit.
    PROSITEiPS50118. HMG_BOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11633-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAATKEAKQP KEPKKRTTRR KKDPNAPKRG LSAYMFFANE NRDIVRSENP   50
    DVTFGQVGRI LGERWKALTA EEKQPYESKA QADKKRYESE KELYNATRA 99
    Length:99
    Mass (Da):11,476
    Last modified:July 27, 2011 - v3
    Checksum:i0243FDC7D19B8A59
    GO

    Sequence cautioni

    The sequence X78993 differs from that shown. Reason: Frameshift at position 30.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 301G → R in X78993. (PubMed:7900426)Curated
    Sequence conflicti30 – 301G → R in CAA85042. (PubMed:7813418)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15318 Genomic DNA. Translation: CAA33378.1.
    X78993 Genomic DNA. No translation available.
    Z35957 Genomic DNA. Translation: CAA85040.1.
    Z35959 Genomic DNA. Translation: CAA85042.1.
    AY558566 Genomic DNA. Translation: AAS56892.1.
    EF123125 mRNA. Translation: ABM97469.1.
    BK006936 Genomic DNA. Translation: DAA07210.2.
    PIRiS78076.
    RefSeqiNP_009647.2. NM_001180058.2.

    Genome annotation databases

    EnsemblFungiiYBR089C-A; YBR089C-A; YBR089C-A.
    GeneIDi852386.
    KEGGisce:YBR089C-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15318 Genomic DNA. Translation: CAA33378.1 .
    X78993 Genomic DNA. No translation available.
    Z35957 Genomic DNA. Translation: CAA85040.1 .
    Z35959 Genomic DNA. Translation: CAA85042.1 .
    AY558566 Genomic DNA. Translation: AAS56892.1 .
    EF123125 mRNA. Translation: ABM97469.1 .
    BK006936 Genomic DNA. Translation: DAA07210.2 .
    PIRi S78076.
    RefSeqi NP_009647.2. NM_001180058.2.

    3D structure databases

    ProteinModelPortali P11633.
    SMRi P11633. Positions 10-99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32795. 99 interactions.
    DIPi DIP-6748N.
    IntActi P11633. 23 interactions.
    MINTi MINT-615533.
    STRINGi 4932.YBR089C-A.

    Proteomic databases

    PaxDbi P11633.
    PeptideAtlasi P11633.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR089C-A ; YBR089C-A ; YBR089C-A .
    GeneIDi 852386.
    KEGGi sce:YBR089C-A.

    Organism-specific databases

    CYGDi YBR089c-a.
    SGDi S000002157. NHP6B.

    Phylogenomic databases

    eggNOGi COG5648.
    GeneTreei ENSGT00740000115458.
    HOGENOMi HOG000197861.
    OMAi LANPNNK.
    OrthoDBi EOG70KH25.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29229-MONOMER.

    Miscellaneous databases

    NextBioi 971195.

    Gene expression databases

    Genevestigatori P11633.

    Family and domain databases

    Gene3Di 1.10.30.10. 1 hit.
    InterProi IPR009071. HMG_box_dom.
    [Graphical view ]
    Pfami PF00505. HMG_box. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 1 hit.
    PROSITEi PS50118. HMG_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Duplicated NHP6 genes of Saccharomyces cerevisiae encode proteins homologous to bovine high mobility group protein 1."
      Kolodrubetz D., Burgum A.
      J. Biol. Chem. 265:3234-3239(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DBY1091 / DKY1.
    2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 30.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
      Juneau K., Palm C., Miranda M., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-57.
      Strain: ATCC 201390 / BY4743.
    7. "DNA looping by Saccharomyces cerevisiae high mobility group proteins NHP6A/B. Consequences for nucleoprotein complex assembly and chromatin condensation."
      Paull T.T., Johnson R.C.
      J. Biol. Chem. 270:8744-8754(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro."
      Paull T.T., Carey M., Johnson R.C.
      Genes Dev. 10:2769-2781(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B."
      Moreira J.M.A., Holmberg S.
      EMBO J. 19:6804-6813(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN."
      Formosa T., Eriksson P., Wittmeyer J., Ginn J., Yu Y., Stillman D.J.
      EMBO J. 20:3506-3517(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE SPT16-POB3 DIMER AND NUCLEOSOMES, FUNCTION OF THE FACT COMPLEX.
    11. "Nhp6, an HMG1 protein, functions in SNR6 transcription by RNA polymerase III in S. cerevisiae."
      Kruppa M., Moir R.D., Kolodrubetz D., Willis I.M.
      Mol. Cell 7:309-318(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. Erratum
      Kruppa M., Moir R.D., Kolodrubetz D., Willis I.M.
      Mol. Cell 8:727-727(2001)
    13. "High-mobility-group proteins NHP6A and NHP6B participate in activation of the RNA polymerase III SNR6 gene."
      Lopez S., Livingstone-Zatchej M., Jourdain S., Thoma F., Sentenac A., Marsolier M.-C.
      Mol. Cell. Biol. 21:3096-3104(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "A bipartite yeast SSRP1 analog comprised of Pob3 and Nhp6 proteins modulates transcription."
      Brewster N.K., Johnston G.C., Singer R.A.
      Mol. Cell. Biol. 21:3491-3502(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE SPT16-POB3 DIMER.
    15. "Multiple Nhp6 molecules are required to recruit Spt16-Pob3 to form yFACT complexes and to reorganize nucleosomes."
      Ruone S., Rhoades A.R., Formosa T.
      J. Biol. Chem. 278:45288-45295(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    16. "The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo."
      Mason P.B., Struhl K.
      Mol. Cell. Biol. 23:8323-8333(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE FACT COMPLEX.
    17. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    18. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    19. "Nhp6 is a transcriptional initiation fidelity factor for RNA polymerase III transcription in vitro and in vivo."
      Kassavetis G.A., Steiner D.F.
      J. Biol. Chem. 281:7445-7451(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    20. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNHP6B_YEAST
    AccessioniPrimary (citable) accession number: P11633
    Secondary accession number(s): A2TBM2
    , D6VQ90, P89500, P89501
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 141 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4590 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3