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Protein

Non-histone chromosomal protein 6B

Gene

NHP6B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6A.9 Publications

Miscellaneous

Present with 4590 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi27 – 95HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • DNA binding, bending Source: SGD
  • nucleosome binding Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • DNA repair Source: UniProtKB-KW
  • maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase III promoter Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29229-MONOMER
ReactomeiR-SCE-211227 Activation of DNA fragmentation factor
R-SCE-5686938 Regulation of TLR by endogenous ligand
R-SCE-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Non-histone chromosomal protein 6B
Gene namesi
Name:NHP6B
Synonyms:NHPB
Ordered Locus Names:YBR089C-A
ORF Names:YBR090BC
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR089C-A
SGDiS000002157 NHP6B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000485661 – 99Non-histone chromosomal protein 6BAdd BLAST99

Proteomic databases

PaxDbiP11633
PRIDEiP11633

PTM databases

iPTMnetiP11633

Interactioni

Subunit structurei

Weakly associates with the stable SPT16-POB3 heterodimer to form the FACT (yFACT or SNP) complex, which is associated with nucleosomes. Multiple molecules of NHP6 (NHP6A and/or NHP6B) are required to recruit the SPT16-POB3 heterodimer to DNA.

Protein-protein interaction databases

BioGridi32795, 154 interactors
DIPiDIP-6748N
IntActiP11633, 23 interactors
STRINGi4932.YBR089C-A

Structurei

3D structure databases

ProteinModelPortaliP11633
SMRiP11633
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NHP6 family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119164
HOGENOMiHOG000197861
InParanoidiP11633
OMAiMFFAKEK
OrthoDBiEOG092C5ER2

Family and domain databases

Gene3Di1.10.30.10, 1 hit
InterProiView protein in InterPro
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
PfamiView protein in Pfam
PF00505 HMG_box, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P11633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATKEAKQP KEPKKRTTRR KKDPNAPKRG LSAYMFFANE NRDIVRSENP
60 70 80 90
DVTFGQVGRI LGERWKALTA EEKQPYESKA QADKKRYESE KELYNATRA
Length:99
Mass (Da):11,476
Last modified:July 27, 2011 - v3
Checksum:i0243FDC7D19B8A59
GO

Sequence cautioni

The sequence X78993 differs from that shown. Reason: Frameshift at position 30.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30G → R in X78993 (PubMed:7900426).Curated1
Sequence conflicti30G → R in CAA85042 (PubMed:7813418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15318 Genomic DNA Translation: CAA33378.1
X78993 Genomic DNA No translation available.
Z35957 Genomic DNA Translation: CAA85040.1
Z35959 Genomic DNA Translation: CAA85042.1
AY558566 Genomic DNA Translation: AAS56892.1
EF123125 mRNA Translation: ABM97469.1
BK006936 Genomic DNA Translation: DAA07210.2
PIRiS78076
RefSeqiNP_009647.2, NM_001180058.2

Genome annotation databases

EnsemblFungiiYBR089C-A; YBR089C-A; YBR089C-A
GeneIDi852386
KEGGisce:YBR089C-A

Similar proteinsi

Entry informationi

Entry nameiNHP6B_YEAST
AccessioniPrimary (citable) accession number: P11633
Secondary accession number(s): A2TBM2
, D6VQ90, P89500, P89501
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
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Main funding by: National Institutes of Health