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Protein

Non-histone chromosomal protein 6B

Gene

NHP6B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6A.9 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi27 – 9569HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding, bending Source: SGD
  • nucleosome binding Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • DNA repair Source: UniProtKB-KW
  • maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase III promoter Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29229-MONOMER.
ReactomeiR-SCE-211227. Activation of DNA fragmentation factor.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-histone chromosomal protein 6B
Gene namesi
Name:NHP6B
Synonyms:NHPB
Ordered Locus Names:YBR089C-A
ORF Names:YBR090BC
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR089C-A.
SGDiS000002157. NHP6B.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9999Non-histone chromosomal protein 6BPRO_0000048566Add
BLAST

PTM databases

iPTMnetiP11633.

Interactioni

Subunit structurei

Weakly associates with the stable SPT16-POB3 heterodimer to form the FACT (yFACT or SNP) complex, which is associated with nucleosomes. Multiple molecules of NHP6 (NHP6A and/or NHP6B) are required to recruit the SPT16-POB3 heterodimer to DNA.

GO - Molecular functioni

  • nucleosome binding Source: SGD

Protein-protein interaction databases

BioGridi32795. 100 interactions.
DIPiDIP-6748N.
IntActiP11633. 23 interactions.
MINTiMINT-615533.

Structurei

3D structure databases

ProteinModelPortaliP11633.
SMRiP11633. Positions 10-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NHP6 family.Curated
Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119164.
HOGENOMiHOG000197861.
InParanoidiP11633.
OMAiIRFTEIS.
OrthoDBiEOG092C5ER2.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATKEAKQP KEPKKRTTRR KKDPNAPKRG LSAYMFFANE NRDIVRSENP
60 70 80 90
DVTFGQVGRI LGERWKALTA EEKQPYESKA QADKKRYESE KELYNATRA
Length:99
Mass (Da):11,476
Last modified:July 27, 2011 - v3
Checksum:i0243FDC7D19B8A59
GO

Sequence cautioni

The sequence X78993 differs from that shown. Reason: Frameshift at position 30. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301G → R in X78993 (PubMed:7900426).Curated
Sequence conflicti30 – 301G → R in CAA85042 (PubMed:7813418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15318 Genomic DNA. Translation: CAA33378.1.
X78993 Genomic DNA. No translation available.
Z35957 Genomic DNA. Translation: CAA85040.1.
Z35959 Genomic DNA. Translation: CAA85042.1.
AY558566 Genomic DNA. Translation: AAS56892.1.
EF123125 mRNA. Translation: ABM97469.1.
BK006936 Genomic DNA. Translation: DAA07210.2.
PIRiS78076.
RefSeqiNP_009647.2. NM_001180058.2.

Genome annotation databases

EnsemblFungiiYBR089C-A; YBR089C-A; YBR089C-A.
GeneIDi852386.
KEGGisce:YBR089C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15318 Genomic DNA. Translation: CAA33378.1.
X78993 Genomic DNA. No translation available.
Z35957 Genomic DNA. Translation: CAA85040.1.
Z35959 Genomic DNA. Translation: CAA85042.1.
AY558566 Genomic DNA. Translation: AAS56892.1.
EF123125 mRNA. Translation: ABM97469.1.
BK006936 Genomic DNA. Translation: DAA07210.2.
PIRiS78076.
RefSeqiNP_009647.2. NM_001180058.2.

3D structure databases

ProteinModelPortaliP11633.
SMRiP11633. Positions 10-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32795. 100 interactions.
DIPiDIP-6748N.
IntActiP11633. 23 interactions.
MINTiMINT-615533.

PTM databases

iPTMnetiP11633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR089C-A; YBR089C-A; YBR089C-A.
GeneIDi852386.
KEGGisce:YBR089C-A.

Organism-specific databases

EuPathDBiFungiDB:YBR089C-A.
SGDiS000002157. NHP6B.

Phylogenomic databases

GeneTreeiENSGT00760000119164.
HOGENOMiHOG000197861.
InParanoidiP11633.
OMAiIRFTEIS.
OrthoDBiEOG092C5ER2.

Enzyme and pathway databases

BioCyciYEAST:G3O-29229-MONOMER.
ReactomeiR-SCE-211227. Activation of DNA fragmentation factor.

Miscellaneous databases

PROiP11633.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNHP6B_YEAST
AccessioniPrimary (citable) accession number: P11633
Secondary accession number(s): A2TBM2
, D6VQ90, P89500, P89501
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.