Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P11633 (NHP6B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-histone chromosomal protein 6B
Gene names
Name:NHP6B
Synonyms:NHPB
Ordered Locus Names:YBR089C-A
ORF Names:YBR090BC
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length99 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6A. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.15 Ref.16 Ref.19

Subunit structure

Weakly associates with the stable SPT16-POB3 heterodimer to form the FACT (yFACT or SNP) complex, which is associated with nucleosomes. Multiple molecules of NHP6 (NHP6A and/or NHP6B) are required to recruit the SPT16-POB3 heterodimer to DNA.

Subcellular location

Nucleus. Chromosome Ref.17.

Miscellaneous

Present with 4590 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NHP6 family.

Contains 1 HMG box DNA-binding domain.

Sequence caution

The sequence X78993 differs from that shown. Reason: Frameshift at position 30.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9999Non-histone chromosomal protein 6B
PRO_0000048566

Regions

DNA binding27 – 9569HMG box

Experimental info

Sequence conflict301G → R in X78993. Ref.2
Sequence conflict301G → R in CAA85042. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P11633 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 0243FDC7D19B8A59

FASTA9911,476
        10         20         30         40         50         60 
MAATKEAKQP KEPKKRTTRR KKDPNAPKRG LSAYMFFANE NRDIVRSENP DVTFGQVGRI 

        70         80         90 
LGERWKALTA EEKQPYESKA QADKKRYESE KELYNATRA 

« Hide

References

« Hide 'large scale' references
[1]"Duplicated NHP6 genes of Saccharomyces cerevisiae encode proteins homologous to bovine high mobility group protein 1."
Kolodrubetz D., Burgum A.
J. Biol. Chem. 265:3234-3239(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBY1091 / DKY1.
[2]"Analysis of a 70 kb region on the right arm of yeast chromosome II."
Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 30.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
Juneau K., Palm C., Miranda M., Davis R.W.
Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-57.
Strain: ATCC 201390 / BY4743.
[7]"DNA looping by Saccharomyces cerevisiae high mobility group proteins NHP6A/B. Consequences for nucleoprotein complex assembly and chromatin condensation."
Paull T.T., Johnson R.C.
J. Biol. Chem. 270:8744-8754(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro."
Paull T.T., Carey M., Johnson R.C.
Genes Dev. 10:2769-2781(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B."
Moreira J.M.A., Holmberg S.
EMBO J. 19:6804-6813(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN."
Formosa T., Eriksson P., Wittmeyer J., Ginn J., Yu Y., Stillman D.J.
EMBO J. 20:3506-3517(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION WITH THE SPT16-POB3 DIMER AND NUCLEOSOMES, FUNCTION OF THE FACT COMPLEX.
[11]"Nhp6, an HMG1 protein, functions in SNR6 transcription by RNA polymerase III in S. cerevisiae."
Kruppa M., Moir R.D., Kolodrubetz D., Willis I.M.
Mol. Cell 7:309-318(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]Erratum
Kruppa M., Moir R.D., Kolodrubetz D., Willis I.M.
Mol. Cell 8:727-727(2001)
[13]"High-mobility-group proteins NHP6A and NHP6B participate in activation of the RNA polymerase III SNR6 gene."
Lopez S., Livingstone-Zatchej M., Jourdain S., Thoma F., Sentenac A., Marsolier M.-C.
Mol. Cell. Biol. 21:3096-3104(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"A bipartite yeast SSRP1 analog comprised of Pob3 and Nhp6 proteins modulates transcription."
Brewster N.K., Johnston G.C., Singer R.A.
Mol. Cell. Biol. 21:3491-3502(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION WITH THE SPT16-POB3 DIMER.
[15]"Multiple Nhp6 molecules are required to recruit Spt16-Pob3 to form yFACT complexes and to reorganize nucleosomes."
Ruone S., Rhoades A.R., Formosa T.
J. Biol. Chem. 278:45288-45295(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo."
Mason P.B., Struhl K.
Mol. Cell. Biol. 23:8323-8333(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE FACT COMPLEX.
[17]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[18]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[19]"Nhp6 is a transcriptional initiation fidelity factor for RNA polymerase III transcription in vitro and in vivo."
Kassavetis G.A., Steiner D.F.
J. Biol. Chem. 281:7445-7451(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[20]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15318 Genomic DNA. Translation: CAA33378.1.
X78993 Genomic DNA. No translation available.
Z35957 Genomic DNA. Translation: CAA85040.1.
Z35959 Genomic DNA. Translation: CAA85042.1.
AY558566 Genomic DNA. Translation: AAS56892.1.
EF123125 mRNA. Translation: ABM97469.1.
BK006936 Genomic DNA. Translation: DAA07210.2.
PIRS78076.
RefSeqNP_009647.2. NM_001180058.2.

3D structure databases

ProteinModelPortalP11633.
SMRP11633. Positions 10-99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32795. 99 interactions.
DIPDIP-6748N.
IntActP11633. 23 interactions.
MINTMINT-615533.
STRING4932.YBR089C-A.

Proteomic databases

PaxDbP11633.
PeptideAtlasP11633.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR089C-A; YBR089C-A; YBR089C-A.
GeneID852386.
KEGGsce:YBR089C-A.

Organism-specific databases

CYGDYBR089c-a.
SGDS000002157. NHP6B.

Phylogenomic databases

eggNOGCOG5648.
GeneTreeENSGT00740000115458.
HOGENOMHOG000197861.
OMALANPNNK.
OrthoDBEOG70KH25.

Enzyme and pathway databases

BioCycYEAST:G3O-29229-MONOMER.

Gene expression databases

GenevestigatorP11633.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
InterProIPR009071. HMG_box_dom.
[Graphical view]
PfamPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971195.

Entry information

Entry nameNHP6B_YEAST
AccessionPrimary (citable) accession number: P11633
Secondary accession number(s): A2TBM2 expand/collapse secondary AC list , D6VQ90, P89500, P89501
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families