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Protein

Non-histone chromosomal protein 6A

Gene

NHP6A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6B.11 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi21 – 89HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • DNA binding, bending Source: SGD
  • nucleosome binding Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • DNA replication-independent nucleosome organization Source: SGD
  • maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase III promoter Source: SGD
  • mismatch repair Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34205-MONOMER.
ReactomeiR-SCE-211227. Activation of DNA fragmentation factor.
R-SCE-5686938. Regulation of TLR by endogenous ligand.
R-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-histone chromosomal protein 6A
Gene namesi
Name:NHP6A
Synonyms:NHPA
Ordered Locus Names:YPR052C
ORF Names:YP9499.09C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR052C.
SGDiS000006256. NHP6A.

Subcellular locationi

  • Nucleus
  • Chromosome

  • Note: Colocalizes with both RNA polymerase II and some regions that are not transcribed on chromatin.

GO - Cellular componenti

  • chromosome Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18P → A: Does not affect affinity for DNA. 1 Publication1
Mutagenesisi21P → A: Shows a 4-fold reduced affinity for DNA. 1 Publication1
Mutagenesisi28Y → D: Shows a strongly reduced affinity for linear and circular DNA. 1 Publication1
Mutagenesisi29M → A or D: Unable to form 75 bp microcircles. 1 Publication1
Mutagenesisi30F → V: Does not affect affinity for DNA. 1 Publication1
Mutagenesisi31F → D: Shows a strongly reduced affinity for linear and circular DNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000485651 – 93Non-histone chromosomal protein 6AAdd BLAST93

Proteomic databases

MaxQBiP11632.
PRIDEiP11632.
TopDownProteomicsiP11632.

PTM databases

iPTMnetiP11632.

Interactioni

Subunit structurei

Weakly associates with the stable SPT16-POB3 heterodimer to form the FACT (yFACT or SNP) complex, which is associated with nucleosomes. Multiple molecules of NHP6 (NHP6A and/or NHP6B) are required to recruit the SPT16-POB3 heterodimer to DNA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
STH1P325973EBI-12019,EBI-18410

Protein-protein interaction databases

BioGridi36227. 136 interactors.
IntActiP11632. 61 interactors.
MINTiMINT-8285506.

Structurei

Secondary structure

193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi17 – 19Combined sources3
Helixi27 – 35Combined sources9
Turni38 – 42Combined sources5
Helixi48 – 51Combined sources4
Turni52 – 54Combined sources3
Helixi55 – 60Combined sources6
Helixi63 – 74Combined sources12
Helixi77 – 80Combined sources4
Helixi83 – 91Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CG7NMR-A1-93[»]
1J5NNMR-A1-93[»]
1LWMNMR-A1-93[»]
DisProtiDP00432.
ProteinModelPortaliP11632.
SMRiP11632.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11632.

Family & Domainsi

Sequence similaritiesi

Belongs to the NHP6 family.Curated
Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119164.
HOGENOMiHOG000197861.
InParanoidiP11632.
OMAiPEEKQPY.
OrthoDBiEOG092C5ER2.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTPREPKKR TTRKKKDPNA PKRALSAYMF FANENRDIVR SENPDITFGQ
60 70 80 90
VGKKLGEKWK ALTPEEKQPY EAKAQADKKR YESEKELYNA TLA
Length:93
Mass (Da):10,802
Last modified:October 1, 1989 - v1
Checksum:iA227296A12D440D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15317 Genomic DNA. Translation: CAA33377.1.
M95912 Genomic RNA. Translation: AAA34754.1.
Z49219 Genomic DNA. Translation: CAA89171.1.
Z71255 Genomic DNA. Translation: CAA94998.1.
AY693230 Genomic DNA. Translation: AAT93249.1.
BK006949 Genomic DNA. Translation: DAA11475.1.
PIRiA35072.
RefSeqiNP_015377.1. NM_001184149.1.

Genome annotation databases

EnsemblFungiiYPR052C; YPR052C; YPR052C.
GeneIDi856165.
KEGGisce:YPR052C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15317 Genomic DNA. Translation: CAA33377.1.
M95912 Genomic RNA. Translation: AAA34754.1.
Z49219 Genomic DNA. Translation: CAA89171.1.
Z71255 Genomic DNA. Translation: CAA94998.1.
AY693230 Genomic DNA. Translation: AAT93249.1.
BK006949 Genomic DNA. Translation: DAA11475.1.
PIRiA35072.
RefSeqiNP_015377.1. NM_001184149.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CG7NMR-A1-93[»]
1J5NNMR-A1-93[»]
1LWMNMR-A1-93[»]
DisProtiDP00432.
ProteinModelPortaliP11632.
SMRiP11632.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36227. 136 interactors.
IntActiP11632. 61 interactors.
MINTiMINT-8285506.

PTM databases

iPTMnetiP11632.

Proteomic databases

MaxQBiP11632.
PRIDEiP11632.
TopDownProteomicsiP11632.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR052C; YPR052C; YPR052C.
GeneIDi856165.
KEGGisce:YPR052C.

Organism-specific databases

EuPathDBiFungiDB:YPR052C.
SGDiS000006256. NHP6A.

Phylogenomic databases

GeneTreeiENSGT00760000119164.
HOGENOMiHOG000197861.
InParanoidiP11632.
OMAiPEEKQPY.
OrthoDBiEOG092C5ER2.

Enzyme and pathway databases

BioCyciYEAST:G3O-34205-MONOMER.
ReactomeiR-SCE-211227. Activation of DNA fragmentation factor.
R-SCE-5686938. Regulation of TLR by endogenous ligand.
R-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP11632.
PROiP11632.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNHP6A_YEAST
AccessioniPrimary (citable) accession number: P11632
Secondary accession number(s): D6W459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 30, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.