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Protein

V-type proton ATPase subunit B1

Gene

VHA-B1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: TAIR
  • actin filament capping Source: TAIR
  • ATP hydrolysis coupled proton transport Source: InterPro
  • ATP metabolic process Source: InterPro
  • glucose mediated signaling pathway Source: TAIR
  • negative regulation of actin filament depolymerization Source: TAIR
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciARA:AT1G76030-MONOMER.
ReactomeiR-ATH-1222556. ROS, RNS production in response to bacteria.
R-ATH-77387. Insulin receptor recycling.
R-ATH-917977. Transferrin endocytosis and recycling.

Protein family/group databases

TCDBi3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit B1
Short name:
V-ATPase subunit B1
Alternative name(s):
V-ATPase 57 kDa subunit
Vacuolar H(+)-ATPase subunit B isoform 1
Vacuolar proton pump subunit B1
Gene namesi
Name:VHA-B1
Synonyms:AT57
Ordered Locus Names:At1g76030
ORF Names:T4O12.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G76030.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • Golgi apparatus Source: TAIR
  • membrane Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • proton-transporting V-type ATPase, V1 domain Source: InterPro
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 486485V-type proton ATPase subunit B1PRO_0000144639Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP11574.
PRIDEiP11574.

PTM databases

iPTMnetiP11574.

Expressioni

Gene expression databases

GenevisibleiP11574. AT.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e).

GO - Molecular functioni

  • actin filament binding Source: TAIR

Protein-protein interaction databases

BioGridi29153. 4 interactions.
IntActiP11574. 2 interactions.
STRINGi3702.AT1G76030.1.

Structurei

3D structure databases

ProteinModelPortaliP11574.
SMRiP11574. Positions 21-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiKOG1351. Eukaryota.
COG1156. LUCA.
HOGENOMiHOG000165320.
InParanoidiP11574.
KOiK02147.
OMAiIKPRINY.
PhylomeDBiP11574.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00310. ATP_synth_B_arch.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR005723. ATPase_V1-cplx_bsu.
IPR027417. P-loop_NTPase.
IPR022879. V-ATPase_su_B/beta.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039114. V-ATPsynth_beta/V-ATPase_B. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01040. V-ATPase_V1_B. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11574-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTNDLDIEE GTLEIGMEYR TVSGVAGPLV ILDKVKGPKY QEIVNIRLGD
60 70 80 90 100
GSTRRGQVLE VDGEKAVVQV FEGTSGIDNK FTTVQFTGEV LKTPVSLDML
110 120 130 140 150
GRIFNGSGKP IDNGPPILPE AYLDISGSSI NPSERTYPEE MIQTGISTID
160 170 180 190 200
VMNSIARGQK IPLFSAAGLP HNEIAAQICR QAGLVKRLEK TVDLLEDHGE
210 220 230 240 250
DNFAIVFAAM GVNMETAQFF KRDFEENGSM ERVTLFLNLA NDPTIERIIT
260 270 280 290 300
PRIALTTAEY LAYECGKHVL VILTDMSSYA DALREVSAAR EEVPGRRGYP
310 320 330 340 350
GYMYTDLATI YERAGRIEGR KGSITQIPIL TMPNDDITHP TPDLTGYITE
360 370 380 390 400
GQIYIDRQLH NRQIYPPINV LPSLSRLMKS AIGEGMTRKD HSDVSNQLYA
410 420 430 440 450
NYAIGKDVQA MKAVVGEEAL SSEDLLYLEF LDKFERKFVM QGAYDTRNIF
460 470 480
QSLDLAWTLL RIFPRELLHR IPAKTLDQFY SRDSTS
Length:486
Mass (Da):54,108
Last modified:January 10, 2003 - v2
Checksum:i0EC3B68E92CC9F0A
GO

Sequence cautioni

The sequence AAC36485.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti297 – 2971R → V in CAA82390 (PubMed:8580968).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04185 mRNA. Translation: AAC36485.1. Different initiation.
AC007396 Genomic DNA. Translation: AAF26763.1.
CP002684 Genomic DNA. Translation: AEE35786.1.
AY094424 mRNA. Translation: AAM19797.1.
AY125532 mRNA. Translation: AAM78042.1.
Z29126 mRNA. Translation: CAA82390.1.
PIRiA31886.
G96788.
RefSeqiNP_177729.1. NM_106251.4.
UniGeneiAt.21359.
At.68155.

Genome annotation databases

EnsemblPlantsiAT1G76030.1; AT1G76030.1; AT1G76030.
GeneIDi843934.
GrameneiAT1G76030.1; AT1G76030.1; AT1G76030.
KEGGiath:AT1G76030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04185 mRNA. Translation: AAC36485.1. Different initiation.
AC007396 Genomic DNA. Translation: AAF26763.1.
CP002684 Genomic DNA. Translation: AEE35786.1.
AY094424 mRNA. Translation: AAM19797.1.
AY125532 mRNA. Translation: AAM78042.1.
Z29126 mRNA. Translation: CAA82390.1.
PIRiA31886.
G96788.
RefSeqiNP_177729.1. NM_106251.4.
UniGeneiAt.21359.
At.68155.

3D structure databases

ProteinModelPortaliP11574.
SMRiP11574. Positions 21-480.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29153. 4 interactions.
IntActiP11574. 2 interactions.
STRINGi3702.AT1G76030.1.

Protein family/group databases

TCDBi3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiP11574.

Proteomic databases

PaxDbiP11574.
PRIDEiP11574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G76030.1; AT1G76030.1; AT1G76030.
GeneIDi843934.
GrameneiAT1G76030.1; AT1G76030.1; AT1G76030.
KEGGiath:AT1G76030.

Organism-specific databases

TAIRiAT1G76030.

Phylogenomic databases

eggNOGiKOG1351. Eukaryota.
COG1156. LUCA.
HOGENOMiHOG000165320.
InParanoidiP11574.
KOiK02147.
OMAiIKPRINY.
PhylomeDBiP11574.

Enzyme and pathway databases

BioCyciARA:AT1G76030-MONOMER.
ReactomeiR-ATH-1222556. ROS, RNS production in response to bacteria.
R-ATH-77387. Insulin receptor recycling.
R-ATH-917977. Transferrin endocytosis and recycling.

Miscellaneous databases

PROiP11574.

Gene expression databases

GenevisibleiP11574. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00310. ATP_synth_B_arch.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR005723. ATPase_V1-cplx_bsu.
IPR027417. P-loop_NTPase.
IPR022879. V-ATPase_su_B/beta.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039114. V-ATPsynth_beta/V-ATPase_B. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01040. V-ATPase_V1_B. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence and homologies of the '57-kDa' nucleotide-binding subunit of the vacuolar ATPase from Arabidopsis."
    Manolson M.F., Ouellette B.F.F., Filion M., Poole R.J.
    J. Biol. Chem. 263:17987-17994(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 235-334.
    Strain: cv. Columbia.
    Tissue: Seedling.
  6. "A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase."
    Sze H., Schumacher K., Mueller M.L., Padmanaban S., Taiz L.
    Trends Plant Sci. 7:157-161(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana."
    Shimaoka T., Ohnishi M., Sazuka T., Mitsuhashi N., Hara-Nishimura I., Shimazaki K., Maeshima M., Yokota A., Tomizawa K., Mimura T.
    Plant Cell Physiol. 45:672-683(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVATB1_ARATH
AccessioniPrimary (citable) accession number: P11574
Secondary accession number(s): Q42205, Q8L584, Q9LQR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 10, 2003
Last modified: June 8, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.