Reviewed, UniProtKB/Swiss-Prot P11563 (MCRG_METVO)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase subunit gamma
    EC=2.8.4.1
Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase gamma

Gene names

Name:

mcrG

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Methyl-coenzyme M reductase subunit gamma		PRO_0000147484

Sequences

	Sequence		Length	Mass (Da)
	P11563-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 236BA6C71F5AAA49	FASTA	261	30,132
	10 20 30 40 50 60 MAYKPQFYPS ATKVAENRRN HINPAFELEK LREIPDEDVV KIMGHRQPSE DYKTVHPPLE 70 80 90 100 110 120 EMDLAEDYVR DLVGPINGAK EGHRIRYIQF ADSMYFAPSQ PYDRSRLYMS RFRGVDCGTL 130 140 150 160 170 180 SGRQVVELRE SNLEDISKNY LVDTELFDPA TTGMRGATVH GHSLRLDENG VMFDALQRYE 190 200 210 220 230 240 FDEATGHILY VKDQVGRPWD EPVDVGEPVP QEKLKEITTI YRKDGVAMRD DMEVVEVVKR 250 260 IHRARTLGGY CPLNEIFDTY L

References

[1]	"Comparative analysis of genes encoding methyl coenzyme M reductase in methanogenic bacteria." Klein A., Allmansberger R., Bokranz M., Knaub S., Mueller B., Muth E. Mol. Gen. Genet. 213:409-420(1988) [PubMed: 3185509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07793 Genomic DNA. Translation: CAA30632.1.
PIR	S03260.
3D structure databases
SMR	P11563. Positions 6-250.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.4.1. 20957.
Family and domain databases
InterPro	IPR009024. Me_CoM_Rdtase_Fd-like_fold. IPR003178. Me_CoM_Rdtase_gsu. [Graphical view]
Gene3D	G3DSA:3.90.320.20. MCR_gamma. 1 hit.
Pfam	PF02240. MCR_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDom	PD005845. Me_CoM_Rdtase_gsu. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03259. met_CoM_red_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRG_METVO

Accession

Primary (citable) accession number: P11563

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)