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P11561 (MCRB_METVO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-coenzyme M reductase subunit beta

EC=2.8.4.1
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta
Gene names
Name:mcrB
OrganismMethanococcus voltae
Taxonomic identifier2188 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activity

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactor

Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.

Pathway

One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.

Subunit structure

Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
   Biological processMethanogenesis
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processmethanogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncoenzyme-B sulfoethylthiotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Methyl-coenzyme M reductase subunit beta
PRO_0000147472

Sequences

Sequence LengthMass (Da)Tools
P11561 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: E9E545ED9E48AB3F

FASTA44346,681
        10         20         30         40         50         60 
MVKYEDKICL FDAKGNQVAE DVPLEAISPL NNPTIMGMVK NIKRTVAVNL AGIEESLAKG 

        70         80         90        100        110        120 
KIGGKGCQVP GTNIELDVIG DAEAIADKVK SILQVSAGDD TEVKLINGGK QMAEQVPSKR 

       130        140        150        160        170        180 
LDVAAEYSVS MLSTGMALKE ALITNFNIDM FDGSTVHSAI MGQYPQDMDY AGGNIASLLG 

       190        200        210        220        230        240 
APSKLEGLGY ALRNIPVNHA VATTKKSLMN AIAFSSILEQ TAMFEMGDAV GSFERQHLLG 

       250        260        270        280        290        300 
LAYQGLNADN LVVELVKANA TGTVGSVVNS IVEKAIADGV IVVDKTLGSG FNMYKPADVN 

       310        320        330        340        350        360 
KWNAYAAAGL VAAVMVSCGA ARAAQNVAST ILYYNDILEY ETGLPGVDYG RSMGTAVGFS 

       370        380        390        400        410        420 
FFSHSIYGGG GPGIFNGNHV VTRHSKGFAI PPVCAAMCMD AGTQMFSPEK TSALVGTVYS 

       430        440 
AFDEFREPLK YVIEGALEVQ NKL 

« Hide

References

[1]"Comparative analysis of genes encoding methyl coenzyme M reductase in methanogenic bacteria."
Klein A., Allmansberger R., Bokranz M., Knaub S., Mueller B., Muth E.
Mol. Gen. Genet. 213:409-420(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07793 Genomic DNA. Translation: CAA30630.1.
PIRS03257.

3D structure databases

ProteinModelPortalP11561.
SMRP11561. Positions 3-443.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00646; UER00699.

Family and domain databases

Gene3D1.20.840.10. 2 hits.
3.30.70.470. 1 hit.
InterProIPR022681. MCR_a/b_chain_a-bundle.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR003179. Me_CoM_Rdtase_bsu.
IPR022679. Me_CoM_Rdtase_bsu_C.
IPR022680. Me_CoM_Rdtase_bsu_N.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamPF02241. MCR_beta. 1 hit.
PF02783. MCR_beta_N. 1 hit.
[Graphical view]
PIRSFPIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAMSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsTIGR03257. met_CoM_red_bet. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMCRB_METVO
AccessionPrimary (citable) accession number: P11561
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 16, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways