Reviewed, UniProtKB/Swiss-Prot P11561 (MCRB_METVO)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase subunit beta
    EC=2.8.4.1
Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase beta

Gene names

Name:

mcrB

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Methyl-coenzyme M reductase subunit beta		PRO_0000147472

Sequences

	Sequence		Length	Mass (Da)
	P11561-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: E9E545ED9E48AB3F	FASTA	443	46,681
	10 20 30 40 50 60 MVKYEDKICL FDAKGNQVAE DVPLEAISPL NNPTIMGMVK NIKRTVAVNL AGIEESLAKG 70 80 90 100 110 120 KIGGKGCQVP GTNIELDVIG DAEAIADKVK SILQVSAGDD TEVKLINGGK QMAEQVPSKR 130 140 150 160 170 180 LDVAAEYSVS MLSTGMALKE ALITNFNIDM FDGSTVHSAI MGQYPQDMDY AGGNIASLLG 190 200 210 220 230 240 APSKLEGLGY ALRNIPVNHA VATTKKSLMN AIAFSSILEQ TAMFEMGDAV GSFERQHLLG 250 260 270 280 290 300 LAYQGLNADN LVVELVKANA TGTVGSVVNS IVEKAIADGV IVVDKTLGSG FNMYKPADVN 310 320 330 340 350 360 KWNAYAAAGL VAAVMVSCGA ARAAQNVAST ILYYNDILEY ETGLPGVDYG RSMGTAVGFS 370 380 390 400 410 420 FFSHSIYGGG GPGIFNGNHV VTRHSKGFAI PPVCAAMCMD AGTQMFSPEK TSALVGTVYS 430 440 AFDEFREPLK YVIEGALEVQ NKL

References

[1]	"Comparative analysis of genes encoding methyl coenzyme M reductase in methanogenic bacteria." Klein A., Allmansberger R., Bokranz M., Knaub S., Mueller B., Muth E. Mol. Gen. Genet. 213:409-420(1988) [PubMed: 3185509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07793 Genomic DNA. Translation: CAA30630.1.
PIR	S03257.
3D structure databases
HSSP	HSSP built from PDB template 1HBN based on UniProtKB P11560.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.4.1. 20957.
Family and domain databases
InterPro	IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR003179. Me_CoM_Rdtase_bsu. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 2 hits.
Pfam	PF02241. MCR_beta. 1 hit. PF02783. MCR_beta_N. 1 hit. [Graphical view]
PIRSF	PIRSF000263. Meth_CoM_rd_beta. 1 hit.
TIGRFAMs	TIGR03257. met_CoM_red_bet. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRB_METVO

Accession

Primary (citable) accession number: P11561

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	September 22, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)