Reviewed, UniProtKB/Swiss-Prot P11559 (MCRA_METVO)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase subunit alpha
    EC=2.8.4.1
Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase alpha

Gene names

Name:

mcrA

Organism

Methanococcus voltae

Taxonomic identifier

2188 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
Biological process	Methanogenesis
Ligand	Metal-binding Nickel
Molecular function	Transferase
PTM	Methylation
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Methyl-coenzyme M reductase subunit alpha		PRO_0000147461
Sites
	Metal binding	152	1	Nickel By similarity
Amino acid modifications
	Modified residue	262	1	Pros-methylhistidine By similarity
	Modified residue	275	1	5-methylarginine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11559-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: DCBCE5DC91EA69D5	FASTA	555	61,227
	10 20 30 40 50 60 MEAEKRLFLK ALKEKFEEDP KEKFTKFYTY GGWQQSARKQ EFVSENEKIV AEKRGGIPMY 70 80 90 100 110 120 NPDIGVPLGQ RKLMPYKISN TDTYVEGDDL HFMNNAAIQQ LWDDIRRTVI VGMDTAHMVL 130 140 150 160 170 180 EKRLGVEVTP ETINEYMHTI NHSLPGGAAV VQEHMVEVHP SLAWDCYAKI FTGDDELADE 190 200 210 220 230 240 LDDRFVIDIN KLFPEEQAEA SKAAIGKKTY QVSRVPSLVG RVCDGGTISR WSAMQIGMSF 250 260 270 280 290 300 ITAYKLCAGE AAIADFSYAA KHADVIQMGN ALLGRRARGP NELGGVRFGI LSDVVQTTRV 310 320 330 340 350 360 SDDPVEQSLE VVATGAALYD QIWLGSYMSG GVGFTQYATA SYTDDILDDF SYYGYEYVEK 370 380 390 400 410 420 KYGRCGTKAT MDVVEDIASE VTLYALEQYD EYPALLEDHF GGSQRAAVAA AAAGISVCMA 430 440 450 460 470 480 TGNSNAGVNG WYLSQILHKE YHSRLGFYGY DLQDQCGASN SLAIRNDEAS PLELRGPNYP 490 500 510 520 530 540 NYAMNVGHQG EYAGIAQSAH SARGDAFATN ALIKVAFADP SLVFDFSKPR KEIARGALRE 550 FEAAGERDPI LPAKI

References

[1]	"Comparative analysis of genes encoding methyl coenzyme M reductase in methanogenic bacteria." Klein A., Allmansberger R., Bokranz M., Knaub S., Mueller B., Muth E. Mol. Gen. Genet. 213:409-420(1988) [PubMed: 3185509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1537 / PS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07793 Genomic DNA. Translation: CAA30633.1.
PIR	S03261.
3D structure databases
HSSP	HSSP built from PDB template 1E6V based on UniProtKB Q49605.
SMR	P11559. Positions 6-553.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.4.1. 20957.
Family and domain databases
InterPro	IPR016212. Me_CoM_Rdtase_asu. IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR009047. Me_CoM_Rdtase_asu_C. IPR003183. Me_CoM_Rdtase_asu_N. IPR015811. Me_CoM_Rdtase_asu_N_sub1. IPR015823. Me_CoM_Rdtase_asu_N_sub2. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 1 hit. G3DSA:3.90.390.10. Me_CoM_Rdtase_asu_N_sub1. 1 hit. G3DSA:3.30.70.470. Me_CoM_Rdtase_asu_N_sub2. 1 hit.
Pfam	PF02249. MCR_alpha. 1 hit. PF02745. MCR_alpha_N. 1 hit. [Graphical view]
PIRSF	PIRSF000262. MCR_alpha. 1 hit.
TIGRFAMs	TIGR03256. met_CoM_red_alp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRA_METVO

Accession

Primary (citable) accession number: P11559

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	September 22, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)