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Protein

Cell division protein DamX

Gene

damX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-essential cell division protein.UniRule annotation2 Publications

Miscellaneous

Overexpression induces cell filamentation.1 Publication

GO - Molecular functioni

  • peptidoglycan binding Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG11183-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein DamXUniRule annotationCurated
Gene namesi
Name:damXUniRule annotation
Synonyms:yhfB
Ordered Locus Names:b3388, JW3351
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11183 damX

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 103Cytoplasmic1 PublicationAdd BLAST103
Transmembranei104 – 124HelicalUniRule annotationAdd BLAST21
Topological domaini125 – 428Periplasmic1 PublicationAdd BLAST304

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • cell septum Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion mutant shows no obvious phenotype, but absence of the protein aggravates the division defect in ftsN or dedD mutants.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi351Q → K: Eliminates septal localization. 1 Publication1
Mutagenesisi354S → K: Impairs septal localization. 1 Publication1
Mutagenesisi416W → L: Impairs septal localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000797791 – 428Cell division protein DamXAdd BLAST428

Proteomic databases

PaxDbiP11557
PRIDEiP11557

Interactioni

Subunit structurei

Interacts in vitro with multiple Fts proteins, including FtsQ and FtsN.1 Publication

Protein-protein interaction databases

BioGridi4262961, 603 interactors
DIPiDIP-9397N
IntActiP11557, 22 interactors
STRINGi316385.ECDH10B_3563

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi358 – 367Combined sources10
Beta strandi380 – 383Combined sources4
Beta strandi393 – 395Combined sources3
Helixi396 – 405Combined sources10
Helixi408 – 411Combined sources4
Turni420 – 422Combined sources3
Helixi423 – 425Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LFVNMR-A338-428[»]
ProteinModelPortaliP11557
SMRiP11557
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini342 – 419SPORUniRule annotationAdd BLAST78

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili55 – 87UniRule annotationAdd BLAST33

Domaini

The SPOR domain binds septal peptidoglycans and is required to target DamX to the septal ring.UniRule annotation2 Publications

Sequence similaritiesi

Belongs to the DamX family.UniRule annotationCurated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGB Bacteria
COG3266 LUCA
HOGENOMiHOG000125518
InParanoidiP11557
KOiK03112
OMAiASRQYIM

Family and domain databases

Gene3Di3.30.70.1070, 1 hit
HAMAPiMF_02021 DamX, 1 hit
InterProiView protein in InterPro
IPR032899 DamX
IPR007730 SPOR-like_dom
IPR036680 SPOR-like_sf
PfamiView protein in Pfam
PF05036 SPOR, 1 hit
SUPFAMiSSF110997 SSF110997, 1 hit
PROSITEiView protein in PROSITE
PS51724 SPOR, 1 hit

Sequencei

Sequence statusi: Complete.

P11557-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEFKPEDEL KPDPSDRRTG RSRQSSERSE RTERGEPQIN FDDIELDDTD
60 70 80 90 100
DRRPTRAQKE RNEEPEIEEE IDESEDETVD EERVERRPRK RKKAASKPAS
110 120 130 140 150
RQYMMMGVGI LVLLLLIIGI GSALKAPSTT SSDQTASGEK SIDLAGNATD
160 170 180 190 200
QANGVQPAPG TTSAENTQQD VSLPPISSTP TQGQTPVATD GQQRVEVQGD
210 220 230 240 250
LNNALTQPQN QQQLNNVAVN STLPTEPATV APVRNGNASR DTAKTQTAER
260 270 280 290 300
PSTTRPARQQ AVIEPKKPQA TVKTEPKPVA QTPKRTEPAA PVASTKAPAA
310 320 330 340 350
TSTPAPKETA TTAPVQTASP AQTTATPAAG AKTAGNVGSL KSAPSSHYTL
360 370 380 390 400
QLSSSSNYDN LNGWAKKENL KNYVVYETTR NGQPWYVLVS GVYASKEEAK
410 420
KAVSTLPADV QAKNPWAKPL RQVQADLK
Length:428
Mass (Da):46,162
Last modified:August 29, 2003 - v2
Checksum:iE3376F88383BE94A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6P → T in CAA33253 (PubMed:2549371).Curated1
Sequence conflicti6P → T in CAA79667 (PubMed:7603433).Curated1
Sequence conflicti31R → P in CAA33253 (PubMed:2549371).Curated1
Sequence conflicti31R → P in CAA79667 (PubMed:7603433).Curated1
Sequence conflicti86 – 91RRPRKR → LVRVS in CAA33253 (PubMed:2549371).Curated6
Sequence conflicti86 – 91RRPRKR → LVRVS in CAA79667 (PubMed:7603433).Curated6
Sequence conflicti233V → L in CAA79667 (PubMed:7603433).Curated1
Sequence conflicti310A → V in CAA33253 (PubMed:2549371).Curated1
Sequence conflicti310A → V in CAA79667 (PubMed:7603433).Curated1
Sequence conflicti366K → N in CAA79667 (PubMed:7603433).Curated1
Sequence conflicti382G → V in CAA33253 (PubMed:2549371).Curated1
Sequence conflicti382G → V in CAA79667 (PubMed:7603433).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15162 Genomic DNA Translation: CAA33253.1
Z19601 Genomic DNA Translation: CAA79667.1
U18997 Genomic DNA Translation: AAA58185.1
U00096 Genomic DNA Translation: AAC76413.1
AP009048 Genomic DNA Translation: BAE77903.1
PIRiG65133 Q4ECAD
RefSeqiNP_417847.1, NC_000913.3
WP_000343215.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76413; AAC76413; b3388
BAE77903; BAE77903; BAE77903
GeneIDi947930
KEGGiecj:JW3351
eco:b3388
PATRICifig|1411691.4.peg.3342

Similar proteinsi

Entry informationi

Entry nameiDAMX_ECOLI
AccessioniPrimary (citable) accession number: P11557
Secondary accession number(s): P76687, Q2M753
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health