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Protein

Cell division protein DamX

Gene

damX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-essential cell division protein.UniRule annotation2 Publications

GO - Molecular functioni

  • peptidoglycan binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG11183-MONOMER.
ECOL316407:JW3351-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein DamXUniRule annotationCurated
Gene namesi
Name:damXUniRule annotation
Synonyms:yhfB
Ordered Locus Names:b3388, JW3351
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11183. damX.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 103103Cytoplasmic1 PublicationAdd
BLAST
Transmembranei104 – 12421HelicalUniRule annotationAdd
BLAST
Topological domaini125 – 428304Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • cell septum Source: EcoCyc
  • integral component of plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion mutant shows no obvious phenotype, but absence of the protein aggravates the division defect in ftsN or dedD mutants.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi351 – 3511Q → K: Eliminates septal localization. 1 Publication
Mutagenesisi354 – 3541S → K: Impairs septal localization. 1 Publication
Mutagenesisi416 – 4161W → L: Impairs septal localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Cell division protein DamXPRO_0000079779Add
BLAST

Proteomic databases

PaxDbiP11557.
PRIDEiP11557.

Interactioni

Subunit structurei

Interacts in vitro with multiple Fts proteins, including FtsQ and FtsN.1 Publication

Protein-protein interaction databases

BioGridi4262961. 603 interactions.
DIPiDIP-9397N.
IntActiP11557. 22 interactions.
STRINGi511145.b3388.

Structurei

Secondary structure

1
428
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi358 – 36710Combined sources
Beta strandi380 – 3834Combined sources
Beta strandi393 – 3953Combined sources
Helixi396 – 40510Combined sources
Helixi408 – 4114Combined sources
Turni420 – 4223Combined sources
Helixi423 – 4253Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LFVNMR-A338-428[»]
ProteinModelPortaliP11557.
SMRiP11557. Positions 340-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini342 – 41978SPORUniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili55 – 8733UniRule annotationAdd
BLAST

Domaini

The SPOR domain binds septal peptidoglycans and is required to target DamX to the septal ring.UniRule annotation2 Publications

Sequence similaritiesi

Belongs to the DamX family.UniRule annotationCurated
Contains 1 SPOR domain.UniRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EGB. Bacteria.
COG3266. LUCA.
HOGENOMiHOG000125518.
InParanoidiP11557.
KOiK03112.
OMAiASRQYIM.

Family and domain databases

Gene3Di3.30.70.1070. 1 hit.
HAMAPiMF_02021. DamX. 1 hit.
InterProiIPR032899. DamX.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF05036. SPOR. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
PROSITEiPS51724. SPOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11557-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEFKPEDEL KPDPSDRRTG RSRQSSERSE RTERGEPQIN FDDIELDDTD
60 70 80 90 100
DRRPTRAQKE RNEEPEIEEE IDESEDETVD EERVERRPRK RKKAASKPAS
110 120 130 140 150
RQYMMMGVGI LVLLLLIIGI GSALKAPSTT SSDQTASGEK SIDLAGNATD
160 170 180 190 200
QANGVQPAPG TTSAENTQQD VSLPPISSTP TQGQTPVATD GQQRVEVQGD
210 220 230 240 250
LNNALTQPQN QQQLNNVAVN STLPTEPATV APVRNGNASR DTAKTQTAER
260 270 280 290 300
PSTTRPARQQ AVIEPKKPQA TVKTEPKPVA QTPKRTEPAA PVASTKAPAA
310 320 330 340 350
TSTPAPKETA TTAPVQTASP AQTTATPAAG AKTAGNVGSL KSAPSSHYTL
360 370 380 390 400
QLSSSSNYDN LNGWAKKENL KNYVVYETTR NGQPWYVLVS GVYASKEEAK
410 420
KAVSTLPADV QAKNPWAKPL RQVQADLK
Length:428
Mass (Da):46,162
Last modified:August 29, 2003 - v2
Checksum:iE3376F88383BE94A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61P → T in CAA33253 (PubMed:2549371).Curated
Sequence conflicti6 – 61P → T in CAA79667 (PubMed:7603433).Curated
Sequence conflicti31 – 311R → P in CAA33253 (PubMed:2549371).Curated
Sequence conflicti31 – 311R → P in CAA79667 (PubMed:7603433).Curated
Sequence conflicti86 – 916RRPRKR → LVRVS in CAA33253 (PubMed:2549371).Curated
Sequence conflicti86 – 916RRPRKR → LVRVS in CAA79667 (PubMed:7603433).Curated
Sequence conflicti233 – 2331V → L in CAA79667 (PubMed:7603433).Curated
Sequence conflicti310 – 3101A → V in CAA33253 (PubMed:2549371).Curated
Sequence conflicti310 – 3101A → V in CAA79667 (PubMed:7603433).Curated
Sequence conflicti366 – 3661K → N in CAA79667 (PubMed:7603433).Curated
Sequence conflicti382 – 3821G → V in CAA33253 (PubMed:2549371).Curated
Sequence conflicti382 – 3821G → V in CAA79667 (PubMed:7603433).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15162 Genomic DNA. Translation: CAA33253.1.
Z19601 Genomic DNA. Translation: CAA79667.1.
U18997 Genomic DNA. Translation: AAA58185.1.
U00096 Genomic DNA. Translation: AAC76413.1.
AP009048 Genomic DNA. Translation: BAE77903.1.
PIRiG65133. Q4ECAD.
RefSeqiNP_417847.1. NC_000913.3.
WP_000343215.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76413; AAC76413; b3388.
BAE77903; BAE77903; BAE77903.
GeneIDi947930.
KEGGiecj:JW3351.
eco:b3388.
PATRICi32122208. VBIEscCol129921_3481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15162 Genomic DNA. Translation: CAA33253.1.
Z19601 Genomic DNA. Translation: CAA79667.1.
U18997 Genomic DNA. Translation: AAA58185.1.
U00096 Genomic DNA. Translation: AAC76413.1.
AP009048 Genomic DNA. Translation: BAE77903.1.
PIRiG65133. Q4ECAD.
RefSeqiNP_417847.1. NC_000913.3.
WP_000343215.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LFVNMR-A338-428[»]
ProteinModelPortaliP11557.
SMRiP11557. Positions 340-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262961. 603 interactions.
DIPiDIP-9397N.
IntActiP11557. 22 interactions.
STRINGi511145.b3388.

Proteomic databases

PaxDbiP11557.
PRIDEiP11557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76413; AAC76413; b3388.
BAE77903; BAE77903; BAE77903.
GeneIDi947930.
KEGGiecj:JW3351.
eco:b3388.
PATRICi32122208. VBIEscCol129921_3481.

Organism-specific databases

EchoBASEiEB1170.
EcoGeneiEG11183. damX.

Phylogenomic databases

eggNOGiENOG4105EGB. Bacteria.
COG3266. LUCA.
HOGENOMiHOG000125518.
InParanoidiP11557.
KOiK03112.
OMAiASRQYIM.

Enzyme and pathway databases

BioCyciEcoCyc:EG11183-MONOMER.
ECOL316407:JW3351-MONOMER.

Miscellaneous databases

PROiP11557.

Family and domain databases

Gene3Di3.30.70.1070. 1 hit.
HAMAPiMF_02021. DamX. 1 hit.
InterProiIPR032899. DamX.
IPR007730. SPOR_dom.
[Graphical view]
PfamiPF05036. SPOR. 1 hit.
[Graphical view]
SUPFAMiSSF110997. SSF110997. 1 hit.
PROSITEiPS51724. SPOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAMX_ECOLI
AccessioniPrimary (citable) accession number: P11557
Secondary accession number(s): P76687, Q2M753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 29, 2003
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression induces cell filamentation.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.