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Protein

L-fuculokinase

Gene

fucK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of L-fuculose. Can also phosphorylate, with lower efficiency, D-ribulose, D-xylulose and D-fructose.UniRule annotation1 Publication

Catalytic activityi

ATP + L-fuculose = ADP + L-fuculose 1-phosphate.UniRule annotation1 Publication

Cofactori

a divalent metal cationUniRule annotation1 Publication

pH dependencei

Optimum pH is 7.8.1 Publication

Pathwayi: L-fucose degradation

This protein is involved in step 2 of the subpathway that synthesizes L-lactaldehyde and glycerone phosphate from L-fucose.UniRule annotation1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. L-fucose isomerase (fucI), L-fucose isomerase (fucI)
  2. L-fuculokinase (fucK), L-fuculokinase (fucK)
  3. L-fuculose phosphate aldolase (fucA), L-fuculose phosphate aldolase (fucA)
This subpathway is part of the pathway L-fucose degradation, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lactaldehyde and glycerone phosphate from L-fucose, the pathway L-fucose degradation and in Carbohydrate degradation.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • L-fuculokinase activity Source: EcoCyc

GO - Biological processi

  • carbohydrate phosphorylation Source: GOC
  • D-arabinose catabolic process Source: EcoCyc
  • L-fucose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:FUCULOKIN-MONOMER.
ECOL316407:JW2774-MONOMER.
MetaCyc:FUCULOKIN-MONOMER.
UniPathwayiUPA00563; UER00625.

Names & Taxonomyi

Protein namesi
Recommended name:
L-fuculokinaseUniRule annotation (EC:2.7.1.51UniRule annotation)
Alternative name(s):
L-fuculose kinaseUniRule annotation
Gene namesi
Name:fucKUniRule annotation
Ordered Locus Names:b2803, JW2774
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10350. fucK.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472L-fuculokinasePRO_0000059423Add
BLAST

Proteomic databases

PaxDbiP11553.
PRIDEiP11553.

2D gel databases

SWISS-2DPAGEP11553.

Interactioni

Protein-protein interaction databases

BioGridi4259225. 22 interactions.
DIPiDIP-9712N.
IntActiP11553. 4 interactions.
MINTiMINT-1305054.
STRINGi511145.b2803.

Structurei

3D structure databases

ProteinModelPortaliP11553.
SMRiP11553. Positions 6-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CMG. Bacteria.
COG1070. LUCA.
HOGENOMiHOG000124178.
InParanoidiP11553.
KOiK00879.
OMAiATNVRAM.
OrthoDBiEOG6X9MHN.

Family and domain databases

HAMAPiMF_00986. Fuculokinase.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR013450. Fuculokinase.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR02628. fuculo_kin_coli. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQEVILVLD CGATNVRAIA VNRQGKIVAR ASTPNASDIA MENNTWHQWS
60 70 80 90 100
LDAILQRFAD CCRQINSELT ECHIRGIAVT TFGVDGALVD KQGNLLYPII
110 120 130 140 150
SWKCPRTAAV MDNIERLISA QRLQAISGVG AFSFNTLYKL VWLKENHPQL
160 170 180 190 200
LERAHAWLFI SSLINHRLTG EFTTDITMAG TSQMLDIQQR DFSPQILQAT
210 220 230 240 250
GIPRRLFPRL VEAGEQIGTL QNSAAAMLGL PVGIPVISAG HDTQFALFGA
260 270 280 290 300
GAEQNEPVLS SGTWEILMVR SAQVDTSLLS QYAGSTCELD SQAGLYNPGM
310 320 330 340 350
QWLASGVLEW VRKLFWTAET PWQMLIEEAR LIAPGADGVK MQCDLLSCQN
360 370 380 390 400
AGWQGVTLNT TRGHFYRAAL EGLTAQLQRN LQMLEKIGHF KASELLLVGG
410 420 430 440 450
GSRNTLWNQI KANMLDIPVK VLDDAETTVA GAALFGWYGV GEFNSPEEAR
460 470
AQIHYQYRYF YPQTEPEFIE EV
Length:472
Mass (Da):52,259
Last modified:October 14, 2015 - v2
Checksum:iE09BB640495D7A3D
GO

Sequence cautioni

The sequence AAB40453.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE76875.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA33128.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15025 Genomic DNA. Translation: CAA33128.1. Different initiation.
U29581 Genomic DNA. Translation: AAB40453.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75845.2.
AP009048 Genomic DNA. Translation: BAE76875.1. Different initiation.
PIRiJS0186. KIECFK.
RefSeqiNP_417283.2. NC_000913.3.
WP_000808392.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75845; AAC75845; b2803.
BAE76875; BAE76875; BAE76875.
GeneIDi946022.
KEGGiecj:JW2774.
eco:b2803.
PATRICi32121022. VBIEscCol129921_2903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15025 Genomic DNA. Translation: CAA33128.1. Different initiation.
U29581 Genomic DNA. Translation: AAB40453.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75845.2.
AP009048 Genomic DNA. Translation: BAE76875.1. Different initiation.
PIRiJS0186. KIECFK.
RefSeqiNP_417283.2. NC_000913.3.
WP_000808392.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP11553.
SMRiP11553. Positions 6-467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259225. 22 interactions.
DIPiDIP-9712N.
IntActiP11553. 4 interactions.
MINTiMINT-1305054.
STRINGi511145.b2803.

2D gel databases

SWISS-2DPAGEP11553.

Proteomic databases

PaxDbiP11553.
PRIDEiP11553.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75845; AAC75845; b2803.
BAE76875; BAE76875; BAE76875.
GeneIDi946022.
KEGGiecj:JW2774.
eco:b2803.
PATRICi32121022. VBIEscCol129921_2903.

Organism-specific databases

EchoBASEiEB0346.
EcoGeneiEG10350. fucK.

Phylogenomic databases

eggNOGiENOG4105CMG. Bacteria.
COG1070. LUCA.
HOGENOMiHOG000124178.
InParanoidiP11553.
KOiK00879.
OMAiATNVRAM.
OrthoDBiEOG6X9MHN.

Enzyme and pathway databases

UniPathwayiUPA00563; UER00625.
BioCyciEcoCyc:FUCULOKIN-MONOMER.
ECOL316407:JW2774-MONOMER.
MetaCyc:FUCULOKIN-MONOMER.

Miscellaneous databases

PROiP11553.

Family and domain databases

HAMAPiMF_00986. Fuculokinase.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR013450. Fuculokinase.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR02628. fuculo_kin_coli. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Escherichia coli genes for L-fucose dissimilation."
    Lu Z., Lin E.C.C.
    Nucleic Acids Res. 17:4883-4884(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The metabolism of L-fucose. I. The purification and properties of L-fuculose kinase."
    Heath E.C., Ghalambor M.A.
    J. Biol. Chem. 237:2423-2426(1962) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY.
    Strain: O111:B4.

Entry informationi

Entry nameiFUCK_ECOLI
AccessioniPrimary (citable) accession number: P11553
Secondary accession number(s): Q2MA31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 14, 2015
Last modified: April 13, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.