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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

Pathwayi: lactose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG)
This subpathway is part of the pathway lactose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate, the pathway lactose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei160Proton donorUniRule annotation1 Publication1
Active sitei375NucleophileUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.85. 2903.
SABIO-RKP11546.
UniPathwayiUPA00542; UER00605.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidase1 PublicationUniRule annotation (EC:3.2.1.85UniRule annotation)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase1 PublicationUniRule annotation
Short name:
PGALaseUniRule annotation
P-beta-Gal1 PublicationUniRule annotation
Short name:
PBGUniRule annotation
Gene namesi
Name:lacG1 PublicationUniRule annotation
Encoded oniPlasmid pUCL131 Publication
Plasmid pLP7121 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638831 – 4686-phospho-beta-galactosidaseAdd BLAST468

Expressioni

Inductioni

By lactose. The operon consists of lacABCDFEGX.1 Publication

Structurei

Secondary structure

1468
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 14Combined sources5
Helixi17 – 20Combined sources4
Helixi26 – 28Combined sources3
Helixi34 – 39Combined sources6
Beta strandi41 – 43Combined sources3
Beta strandi46 – 48Combined sources3
Helixi52 – 65Combined sources14
Beta strandi70 – 74Combined sources5
Helixi77 – 80Combined sources4
Beta strandi84 – 87Combined sources4
Helixi90 – 106Combined sources17
Beta strandi109 – 117Combined sources9
Helixi121 – 125Combined sources5
Helixi128 – 130Combined sources3
Helixi133 – 148Combined sources16
Beta strandi154 – 159Combined sources6
Helixi161 – 169Combined sources9
Beta strandi174 – 176Combined sources3
Helixi182 – 205Combined sources24
Beta strandi209 – 217Combined sources9
Beta strandi221 – 225Combined sources5
Helixi229 – 242Combined sources14
Helixi244 – 251Combined sources8
Helixi257 – 270Combined sources14
Helixi278 – 287Combined sources10
Turni288 – 290Combined sources3
Beta strandi293 – 297Combined sources5
Beta strandi302 – 305Combined sources4
Beta strandi312 – 314Combined sources3
Beta strandi325 – 328Combined sources4
Turni329 – 331Combined sources3
Beta strandi332 – 334Combined sources3
Beta strandi338 – 340Combined sources3
Helixi352 – 364Combined sources13
Helixi366 – 368Combined sources3
Beta strandi371 – 375Combined sources5
Helixi392 – 410Combined sources19
Beta strandi415 – 421Combined sources7
Turni429 – 431Combined sources3
Helixi432 – 434Combined sources3
Beta strandi439 – 442Combined sources4
Turni444 – 446Combined sources3
Beta strandi449 – 451Combined sources3
Helixi453 – 464Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PBGX-ray2.30A/B1-468[»]
2PBGX-ray2.50A1-468[»]
3PBGX-ray2.70A/B1-468[»]
4PBGX-ray2.50A/B1-468[»]
ProteinModelPortaliP11546.
SMRiP11546.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11546.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG. 1 hit.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 2 hits.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS
60 70 80 90 100
DFYHKYPVDL ELAEEYGVNG IRISIAWSRI FPTGYGEVNE KGVEFYHKLF
110 120 130 140 150
AECHKRHVEP FVTLHHFDTP EALHSNGDFL NRENIEHFID YAAFCFEEFP
160 170 180 190 200
EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY DLAKVFQSHH NMMVSHARAV
210 220 230 240 250
KLYKDKGYKG EIGVVHALPT KYPYDPENPA DVRAAELEDI IHNKFILDAT
260 270 280 290 300
YLGHYSDKTM EGVNHILAEN GGELDLRDED FQALDAAKDL NDFLGINYYM
310 320 330 340 350
SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRVAPDYV PRTDWDWIIY
360 370 380 390 400
PEGLYDQIMR VKNDYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKQ
410 420 430 440 450
HLEVLSDAIA DGANVKGYFI WSLMDVFSWS NGYEKRYGLF YVDFDTQERY
460
PKKSAHWYKK LAETQVIE
Length:468
Mass (Da):54,072
Last modified:August 1, 1992 - v2
Checksum:i5ACFC9BB81DF0E90
GO

Sequence cautioni

The sequence AAA26949 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA42986 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti383E → Q in AAA25173 (PubMed:3130294).Curated1
Sequence conflicti387N → K in AAA25173 (PubMed:3130294).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28357 Genomic DNA. Translation: AAA25173.1.
M60447 Genomic DNA. Translation: AAA25183.1.
M19454 Genomic DNA. Translation: AAA26949.1. Different initiation.
X60456 Genomic DNA. Translation: CAA42986.1. Different initiation.
PIRiA37168. GLSOPL.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28357 Genomic DNA. Translation: AAA25173.1.
M60447 Genomic DNA. Translation: AAA25183.1.
M19454 Genomic DNA. Translation: AAA26949.1. Different initiation.
X60456 Genomic DNA. Translation: CAA42986.1. Different initiation.
PIRiA37168. GLSOPL.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PBGX-ray2.30A/B1-468[»]
2PBGX-ray2.50A1-468[»]
3PBGX-ray2.70A/B1-468[»]
4PBGX-ray2.50A/B1-468[»]
ProteinModelPortaliP11546.
SMRiP11546.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00542; UER00605.
BRENDAi3.2.1.85. 2903.
SABIO-RKP11546.

Miscellaneous databases

EvolutionaryTraceiP11546.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG. 1 hit.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 2 hits.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLACG_LACLL
AccessioniPrimary (citable) accession number: P11546
Secondary accession number(s): Q79AQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.