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P11546

- LACG_LACLL

UniProt

P11546 - LACG_LACLL

Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei160 – 1601Proton donor
    Active sitei375 – 3751Nucleophile

    GO - Molecular functioni

    1. 6-phospho-beta-galactosidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lactose catabolic process via tagatose-6-phosphate Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKP11546.
    UniPathwayiUPA00542; UER00605.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-galactosidaseUniRule annotation (EC:3.2.1.85UniRule annotation)
    Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolaseUniRule annotation
    Short name:
    PGALaseUniRule annotation
    P-beta-GalUniRule annotation
    Short name:
    PBGUniRule annotation
    Gene namesi
    Name:lacGUniRule annotation
    Encoded oniPlasmid pUCL131 Publication
    Plasmid pLP7121 Publication
    OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
    Taxonomic identifieri1360 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4684686-phospho-beta-galactosidasePRO_0000063883Add
    BLAST

    Expressioni

    Inductioni

    By lactose. The operon consists of lacABCDFEGX.1 Publication

    Structurei

    Secondary structure

    1
    468
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 145
    Helixi17 – 204
    Helixi26 – 283
    Helixi34 – 396
    Beta strandi41 – 433
    Beta strandi46 – 483
    Helixi52 – 6514
    Beta strandi70 – 745
    Helixi77 – 804
    Beta strandi84 – 874
    Helixi90 – 10617
    Beta strandi109 – 1179
    Helixi121 – 1255
    Helixi128 – 1303
    Helixi133 – 14816
    Beta strandi154 – 1596
    Helixi161 – 1699
    Beta strandi174 – 1763
    Helixi182 – 20524
    Beta strandi209 – 2179
    Beta strandi221 – 2255
    Helixi229 – 24214
    Helixi244 – 2518
    Helixi257 – 27014
    Helixi278 – 28710
    Turni288 – 2903
    Beta strandi293 – 2975
    Beta strandi302 – 3054
    Beta strandi312 – 3143
    Beta strandi325 – 3284
    Turni329 – 3313
    Beta strandi332 – 3343
    Beta strandi338 – 3403
    Helixi352 – 36413
    Helixi366 – 3683
    Beta strandi371 – 3755
    Helixi392 – 41019
    Beta strandi415 – 4217
    Turni429 – 4313
    Helixi432 – 4343
    Beta strandi439 – 4424
    Turni444 – 4463
    Beta strandi449 – 4513
    Helixi453 – 46412

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PBGX-ray2.30A/B1-468[»]
    2PBGX-ray2.50A1-468[»]
    3PBGX-ray2.70A/B1-468[»]
    4PBGX-ray2.50A/B1-468[»]
    ProteinModelPortaliP11546.
    SMRiP11546. Positions 1-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11546.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.UniRule annotation

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    HAMAPiMF_01574. LacG.
    InterProiIPR005928. 6P-beta-galactosidase.
    IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR01233. lacG. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11546-1 [UniParc]FASTAAdd to Basket

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    MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS    50
    DFYHKYPVDL ELAEEYGVNG IRISIAWSRI FPTGYGEVNE KGVEFYHKLF 100
    AECHKRHVEP FVTLHHFDTP EALHSNGDFL NRENIEHFID YAAFCFEEFP 150
    EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY DLAKVFQSHH NMMVSHARAV 200
    KLYKDKGYKG EIGVVHALPT KYPYDPENPA DVRAAELEDI IHNKFILDAT 250
    YLGHYSDKTM EGVNHILAEN GGELDLRDED FQALDAAKDL NDFLGINYYM 300
    SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRVAPDYV PRTDWDWIIY 350
    PEGLYDQIMR VKNDYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKQ 400
    HLEVLSDAIA DGANVKGYFI WSLMDVFSWS NGYEKRYGLF YVDFDTQERY 450
    PKKSAHWYKK LAETQVIE 468
    Length:468
    Mass (Da):54,072
    Last modified:August 1, 1992 - v2
    Checksum:i5ACFC9BB81DF0E90
    GO

    Sequence cautioni

    The sequence AAA26949.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA42986.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti383 – 3831E → Q in AAA25173. (PubMed:3130294)Curated
    Sequence conflicti387 – 3871N → K in AAA25173. (PubMed:3130294)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28357 Genomic DNA. Translation: AAA25173.1.
    M60447 Genomic DNA. Translation: AAA25183.1.
    M19454 Genomic DNA. Translation: AAA26949.1. Different initiation.
    X60456 Genomic DNA. Translation: CAA42986.1. Different initiation.
    PIRiA37168. GLSOPL.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28357 Genomic DNA. Translation: AAA25173.1 .
    M60447 Genomic DNA. Translation: AAA25183.1 .
    M19454 Genomic DNA. Translation: AAA26949.1 . Different initiation.
    X60456 Genomic DNA. Translation: CAA42986.1 . Different initiation.
    PIRi A37168. GLSOPL.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PBG X-ray 2.30 A/B 1-468 [» ]
    2PBG X-ray 2.50 A 1-468 [» ]
    3PBG X-ray 2.70 A/B 1-468 [» ]
    4PBG X-ray 2.50 A/B 1-468 [» ]
    ProteinModelPortali P11546.
    SMRi P11546. Positions 1-468.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00542 ; UER00605 .
    SABIO-RK P11546.

    Miscellaneous databases

    EvolutionaryTracei P11546.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    HAMAPi MF_01574. LacG.
    InterProi IPR005928. 6P-beta-galactosidase.
    IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR01233. lacG. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268."
      Boizet B., Villeval D., Slos P., Novel M., Novel G., Mercenier A.
      Gene 62:249-261(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: L13 / Z268.
      Plasmid: pUCL13
    2. "Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis."
      de Vos W.M., Gasson M.J.
      J. Gen. Microbiol. 135:1833-1846(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
      Strain: 712.
      Plasmid: pLP712
    3. "Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis."
      de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.
      J. Biol. Chem. 265:22554-22560(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPERON STRUCTURE, INDUCTION.
      Strain: MG1820.
    4. "Nonidentity between plasmid and chromosomal copies of ISS1-like sequences in Lactococcus lactis subsp. lactis CNRZ270 and their possible role in chromosomal integration of plasmid genes."
      Huang D.C., Novel M., Huang X.F., Novel G.
      Gene 118:39-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-468.
      Strain: Z270.
    5. "The three-dimensional structure of 6-phospho-beta-galactosidase from Lactococcus lactis."
      Wiesmann C., Beste G., Hengstenberg W., Schulz G.E.
      Structure 3:961-968(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    6. "Crystal structures and mechanism of 6-phospho-beta-galactosidase from Lactococcus lactis."
      Wiesmann C., Hengstenberg W., Schulz G.E.
      J. Mol. Biol. 269:851-860(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

    Entry informationi

    Entry nameiLACG_LACLL
    AccessioniPrimary (citable) accession number: P11546
    Secondary accession number(s): Q79AQ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3