6-phospho-beta-galactosidase - Lactococcus lactis subsp. lactis

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Reviewed, UniProtKB/Swiss-Prot P11546 (LACG_LACLA)

Last modified November 25, 2008. Version 75. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:

lacG

Encoded on

Plasmid pUCL13 Ref.3
Plasmid pMG820 Ref.1

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis)

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

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Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol.
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

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Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 468

468

6-phospho-beta-galactosidase

PRO_0000063883

Sites

Active site

160

Proton donor

Active site

375

Nucleophile

Experimental info

Sequence conflict

383

E → Q in AAA25173. Ref.1

Sequence conflict

387

N → K in AAA25173. Ref.1

Secondary structure

468

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P11546-1 [UniParc].

Last modified August 1, 1992. Version 2.
Checksum: 5ACFC9BB81DF0E90
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FASTA

468

54,072

        10         20         30         40         50         60 
MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYHKYPVDL 

        70         80         90        100        110        120 
ELAEEYGVNG IRISIAWSRI FPTGYGEVNE KGVEFYHKLF AECHKRHVEP FVTLHHFDTP 

       130        140        150        160        170        180 
EALHSNGDFL NRENIEHFID YAAFCFEEFP EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY 

       190        200        210        220        230        240 
DLAKVFQSHH NMMVSHARAV KLYKDKGYKG EIGVVHALPT KYPYDPENPA DVRAAELEDI 

       250        260        270        280        290        300 
IHNKFILDAT YLGHYSDKTM EGVNHILAEN GGELDLRDED FQALDAAKDL NDFLGINYYM 

       310        320        330        340        350        360 
SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRVAPDYV PRTDWDWIIY PEGLYDQIMR 

       370        380        390        400        410        420 
VKNDYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKQ HLEVLSDAIA DGANVKGYFI 

       430        440        450        460 
WSLMDVFSWS NGYEKRYGLF YVDFDTQERY PKKSAHWYKK LAETQVIE

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References

[1]	"Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis." de Vos W.M., Gasson M.J. J. Gen. Microbiol. 135:1833-1846(1989) [PubMed: 2515252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: 712.
[2]	"Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis." de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W. J. Biol. Chem. 265:22554-22560(1990) [PubMed: 2125052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MG1820.
[3]	"Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268." Boizet B., Villeval D., Slos P., Novel M., Novel G., Mercenier A. Gene 62:249-261(1988) [PubMed: 3130294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: L13 / Z268.
[4]	"Nonidentity between plasmid and chromosomal copies of ISS1-like sequences in Lactococcus lactis subsp. lactis CNRZ270 and their possible role in chromosomal integration of plasmid genes." Huang D.C., Novel M., Huang X.F., Novel G. Gene 118:39-46(1992) [PubMed: 1339371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-468. Strain: Z270.
[5]	"The three-dimensional structure of 6-phospho-beta-galactosidase from Lactococcus lactis." Wiesmann C., Beste G., Hengstenberg W., Schulz G.E. Structure 3:961-968(1995) [PubMed: 8535789] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[6]	"Crystal structures and mechanism of 6-phospho-beta-galactosidase from Lactococcus lactis." Wiesmann C., Hengstenberg W., Schulz G.E. J. Mol. Biol. 269:851-860(1997) [PubMed: 9223646] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

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Cross-references

Sequence databases

M28357 Genomic DNA. Translation: AAA25173.1.
M60447 Genomic DNA. Translation: AAA25183.1.
M19454 Genomic DNA. Translation: AAA26949.1. Different initiation.
X60456 Genomic DNA. Translation: CAA42986.1. Different initiation.

PIR

GLSOPL. A37168.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PBG	X-ray	2.30	A/B	1-468	[»]
2PBG	X-ray	2.50	A	1-468	[»]
3PBG	X-ray	2.70	A/B	1-468	[»]
4PBG	X-ray	2.50	A/B	1-468	[»]

ModBase

Search...

Family and domain databases

HAMAP

MF_01574.
[Tree]

InterPro

IPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_sub_cat.
IPR005928. LacG.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

PANTHER

PTHR10353. Glyco_hydro_1. 1 hit.

Pfam

PF00232. Glyco_hydro_1. 1 hit.
[Graphical view]

PRINTS

PR00131. GLHYDRLASE1.

TIGRFAMs

TIGR01233. lacG. 1 hit.

PROSITE

PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P11546.

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Entry information

Entry name

LACG_LACLA

Accession

Primary (citable) accession number: P11546
Secondary accession number(s): Q79AQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	August 1, 1992
Last modified:	November 25, 2008
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Family and domain databases

Other Resources

Entry information

Relevant documents