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P11546

- LACG_LACLL

UniProt

P11546 - LACG_LACLL

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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donor
Active sitei375 – 3751Nucleophile

GO - Molecular functioni

  1. 6-phospho-beta-galactosidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lactose catabolic process via tagatose-6-phosphate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKP11546.
UniPathwayiUPA00542; UER00605.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidaseUniRule annotation (EC:3.2.1.85UniRule annotation)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolaseUniRule annotation
Short name:
PGALaseUniRule annotation
P-beta-GalUniRule annotation
Short name:
PBGUniRule annotation
Gene namesi
Name:lacGUniRule annotation
Encoded oniPlasmid pUCL131 Publication
Plasmid pLP7121 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4684686-phospho-beta-galactosidasePRO_0000063883Add
BLAST

Expressioni

Inductioni

By lactose. The operon consists of lacABCDFEGX.1 Publication

Structurei

Secondary structure

1
468
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 145Combined sources
Helixi17 – 204Combined sources
Helixi26 – 283Combined sources
Helixi34 – 396Combined sources
Beta strandi41 – 433Combined sources
Beta strandi46 – 483Combined sources
Helixi52 – 6514Combined sources
Beta strandi70 – 745Combined sources
Helixi77 – 804Combined sources
Beta strandi84 – 874Combined sources
Helixi90 – 10617Combined sources
Beta strandi109 – 1179Combined sources
Helixi121 – 1255Combined sources
Helixi128 – 1303Combined sources
Helixi133 – 14816Combined sources
Beta strandi154 – 1596Combined sources
Helixi161 – 1699Combined sources
Beta strandi174 – 1763Combined sources
Helixi182 – 20524Combined sources
Beta strandi209 – 2179Combined sources
Beta strandi221 – 2255Combined sources
Helixi229 – 24214Combined sources
Helixi244 – 2518Combined sources
Helixi257 – 27014Combined sources
Helixi278 – 28710Combined sources
Turni288 – 2903Combined sources
Beta strandi293 – 2975Combined sources
Beta strandi302 – 3054Combined sources
Beta strandi312 – 3143Combined sources
Beta strandi325 – 3284Combined sources
Turni329 – 3313Combined sources
Beta strandi332 – 3343Combined sources
Beta strandi338 – 3403Combined sources
Helixi352 – 36413Combined sources
Helixi366 – 3683Combined sources
Beta strandi371 – 3755Combined sources
Helixi392 – 41019Combined sources
Beta strandi415 – 4217Combined sources
Turni429 – 4313Combined sources
Helixi432 – 4343Combined sources
Beta strandi439 – 4424Combined sources
Turni444 – 4463Combined sources
Beta strandi449 – 4513Combined sources
Helixi453 – 46412Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PBGX-ray2.30A/B1-468[»]
2PBGX-ray2.50A1-468[»]
3PBGX-ray2.70A/B1-468[»]
4PBGX-ray2.50A/B1-468[»]
ProteinModelPortaliP11546.
SMRiP11546. Positions 1-468.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11546.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11546-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS
60 70 80 90 100
DFYHKYPVDL ELAEEYGVNG IRISIAWSRI FPTGYGEVNE KGVEFYHKLF
110 120 130 140 150
AECHKRHVEP FVTLHHFDTP EALHSNGDFL NRENIEHFID YAAFCFEEFP
160 170 180 190 200
EVNYWTTFNE IGPIGDGQYL VGKFPPGIKY DLAKVFQSHH NMMVSHARAV
210 220 230 240 250
KLYKDKGYKG EIGVVHALPT KYPYDPENPA DVRAAELEDI IHNKFILDAT
260 270 280 290 300
YLGHYSDKTM EGVNHILAEN GGELDLRDED FQALDAAKDL NDFLGINYYM
310 320 330 340 350
SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRVAPDYV PRTDWDWIIY
360 370 380 390 400
PEGLYDQIMR VKNDYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKQ
410 420 430 440 450
HLEVLSDAIA DGANVKGYFI WSLMDVFSWS NGYEKRYGLF YVDFDTQERY
460
PKKSAHWYKK LAETQVIE
Length:468
Mass (Da):54,072
Last modified:August 1, 1992 - v2
Checksum:i5ACFC9BB81DF0E90
GO

Sequence cautioni

The sequence AAA26949.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA42986.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti383 – 3831E → Q in AAA25173. (PubMed:3130294)Curated
Sequence conflicti387 – 3871N → K in AAA25173. (PubMed:3130294)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28357 Genomic DNA. Translation: AAA25173.1.
M60447 Genomic DNA. Translation: AAA25183.1.
M19454 Genomic DNA. Translation: AAA26949.1. Different initiation.
X60456 Genomic DNA. Translation: CAA42986.1. Different initiation.
PIRiA37168. GLSOPL.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28357 Genomic DNA. Translation: AAA25173.1 .
M60447 Genomic DNA. Translation: AAA25183.1 .
M19454 Genomic DNA. Translation: AAA26949.1 . Different initiation.
X60456 Genomic DNA. Translation: CAA42986.1 . Different initiation.
PIRi A37168. GLSOPL.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PBG X-ray 2.30 A/B 1-468 [» ]
2PBG X-ray 2.50 A 1-468 [» ]
3PBG X-ray 2.70 A/B 1-468 [» ]
4PBG X-ray 2.50 A/B 1-468 [» ]
ProteinModelPortali P11546.
SMRi P11546. Positions 1-468.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00542 ; UER00605 .
SABIO-RK P11546.

Miscellaneous databases

EvolutionaryTracei P11546.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
HAMAPi MF_01574. LacG.
InterProi IPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
TIGRFAMsi TIGR01233. lacG. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268."
    Boizet B., Villeval D., Slos P., Novel M., Novel G., Mercenier A.
    Gene 62:249-261(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: L13 / Z268.
    Plasmid: pUCL13
  2. "Structure and expression of the Lactococcus lactis gene for phospho-beta-galactosidase (lacG) in Escherichia coli and L. lactis."
    de Vos W.M., Gasson M.J.
    J. Gen. Microbiol. 135:1833-1846(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: 712.
    Plasmid: pLP712
  3. "Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis."
    de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.
    J. Biol. Chem. 265:22554-22560(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPERON STRUCTURE, INDUCTION.
    Strain: MG1820.
  4. "Nonidentity between plasmid and chromosomal copies of ISS1-like sequences in Lactococcus lactis subsp. lactis CNRZ270 and their possible role in chromosomal integration of plasmid genes."
    Huang D.C., Novel M., Huang X.F., Novel G.
    Gene 118:39-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-468.
    Strain: Z270.
  5. "The three-dimensional structure of 6-phospho-beta-galactosidase from Lactococcus lactis."
    Wiesmann C., Beste G., Hengstenberg W., Schulz G.E.
    Structure 3:961-968(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  6. "Crystal structures and mechanism of 6-phospho-beta-galactosidase from Lactococcus lactis."
    Wiesmann C., Hengstenberg W., Schulz G.E.
    J. Mol. Biol. 269:851-860(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiLACG_LACLL
AccessioniPrimary (citable) accession number: P11546
Secondary accession number(s): Q79AQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3