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Protein

Ligninase LG5

Gene

GLG5

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.By similarity

Catalytic activityi

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H2O.By similarity
2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4-dimethoxyphenyl)methanol radical + 2 H2O.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation
  • heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.By similarity

Pathwayi: lignin degradation

This protein is involved in the pathway lignin degradation, which is part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the pathway lignin degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei70Transition state stabilizerPROSITE-ProRule annotation1
Active sitei74Proton acceptorPROSITE-ProRule annotation1
Metal bindingi75Calcium 1PROSITE-ProRule annotation1
Metal bindingi92Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi94Calcium 1PROSITE-ProRule annotation1
Metal bindingi96Calcium 1PROSITE-ProRule annotation1
Metal bindingi202Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi203Calcium 2PROSITE-ProRule annotation1
Metal bindingi220Calcium 2PROSITE-ProRule annotation1
Metal bindingi222Calcium 2PROSITE-ProRule annotation1
Metal bindingi225Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi227Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Lignin degradation

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14339.
BRENDAi1.11.1.14. 1380.
UniPathwayiUPA00892.

Protein family/group databases

CAZyiAA2. Auxiliary Activities 2.
PeroxiBasei2411. PcLiP03_BKMF1767.

Names & Taxonomyi

Protein namesi
Recommended name:
Ligninase LG5 (EC:1.11.1.14By similarity)
Alternative name(s):
Diarylpropane peroxidase
Lignin peroxidase
Gene namesi
Name:GLG5
Synonyms:LIP6
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002376822 – 27Sequence analysis6
ChainiPRO_000002376928 – 371Ligninase LG5Add BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 42PROSITE-ProRule annotation
Disulfide bondi41 ↔ 311PROSITE-ProRule annotation
Disulfide bondi61 ↔ 146PROSITE-ProRule annotation
Disulfide bondi275 ↔ 344PROSITE-ProRule annotation
Glycosylationi283N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP11543.

Structurei

3D structure databases

ProteinModelPortaliP11543.
SMRiP11543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Ligninase subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II1B. Eukaryota.
ENOG410ZS1R. LUCA.

Family and domain databases

CDDicd00692. ligninase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
PF11895. Peroxidase_ext. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFKKLLAVL TAALSLRAAQ GAAVEKRATC SNGKVVPAAS CCTWFNVLSD
60 70 80 90 100
IQENLFNGGQ CGAEAHESIR LVFHDAIAIS PAMEPQASSV RGADGSIMIF
110 120 130 140 150
DEIETNFHPN IGLDEIVRLQ KPFVQKHGVT PGDFIAFAGA VALSNCPGAP
160 170 180 190 200
QMNFFTGRAP ATQPAPDGLV PEPFHSVDQI IDRVFDAGEF DELELVWMLS
210 220 230 240 250
AHSVAAANDI DPNIQGLPFD STPGIFDSQF FVETQLAGTG FTGGSNNQGE
260 270 280 290 300
VSSPLPGEMR LQSDFLIARD ARTACEWQSF VNNQSKLVSD FQFIFLALTQ
310 320 330 340 350
LGQDPDAMTD CSAVIPISKP APNNTPGFSF FPPGMTMDDV EQACAETPFP
360 370
TLSTLPGPAT SVARIPPPPG A
Length:371
Mass (Da):39,418
Last modified:October 1, 1989 - v1
Checksum:iF0AE30C69D7EF5C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18794 mRNA. Translation: AAA33734.1.
X55343 Genomic DNA. Translation: CAA39033.1.
M63496 Genomic DNA. Translation: AAA33739.1.
PIRiJN0117. OPJGG5.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18794 mRNA. Translation: AAA33734.1.
X55343 Genomic DNA. Translation: CAA39033.1.
M63496 Genomic DNA. Translation: AAA33739.1.
PIRiJN0117. OPJGG5.

3D structure databases

ProteinModelPortaliP11543.
SMRiP11543.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA2. Auxiliary Activities 2.
PeroxiBasei2411. PcLiP03_BKMF1767.

Proteomic databases

PRIDEiP11543.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410II1B. Eukaryota.
ENOG410ZS1R. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00892.
BioCyciMetaCyc:MONOMER-14339.
BRENDAi1.11.1.14. 1380.

Family and domain databases

CDDicd00692. ligninase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
PF11895. Peroxidase_ext. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIG5_PHACH
AccessioniPrimary (citable) accession number: P11543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.