ID   PDE6A_BOVIN             Reviewed;         859 AA.
AC   P11541;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 76.
DE   RecName: Full=Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha;
DE            Short=GMP-PDE alpha;
DE            EC=3.1.4.35;
DE   AltName: Full=PDE V-B1;
GN   Name=PDE6A; Synonyms=PDEA;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=90169986; PubMed=2155175; DOI=10.1016/0888-7543(90)90567-E;
RA   Pittler S.J., Baehr W., Wasmuth J.J., McConnell D.G., Champagne M.S.,
RA   VanTuinen P., Ledbetter D., Davis R.L.;
RT   "Molecular characterization of human and bovine rod photoreceptor cGMP
RT   phosphodiesterase alpha-subunit and chromosomal localization of the
RT   human gene.";
RL   Genomics 6:272-283(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Retina;
RX   MEDLINE=88082056; PubMed=2826095;
RA   Yu A., Ovchinnikov A., Gubanov V.V., Khramtsov N.V., Akhmedov N.B.,
RA   Ishchenko K.A., Zagranichnyi V.E., Vasilevskaya I.A., Rakitina T.V.,
RA   Atabekova N.V., Barinov A.A., Muradov K.G., Shuvaeva T.M.,
RA   Bystrov N.S., Severtsova I.V., Lipkin V.M.;
RT   "Cyclic GMP phosphodiesterase from the bovine retina. Amino acid
RT   sequence of the alpha-subunit and nucleotide sequence of corresponding
RT   cDNA.";
RL   Dokl. Akad. Nauk SSSR 296:487-491(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND ACETYLATION
RP   AT GLY-2.
RC   TISSUE=Retina;
RX   MEDLINE=88030033; PubMed=2822478; DOI=10.1016/0014-5793(87)80530-6;
RA   Ovchinnikov Y.A., Gubanov V.V., Khramtsov N.V., Ischenko K.A.,
RA   Zagranichny V.E., Muradov K.G., Shuvaeva T.M., Lipkin V.M.;
RT   "Cyclic GMP phosphodiesterase from bovine retina. Amino acid sequence
RT   of the alpha-subunit and nucleotide sequence of the corresponding
RT   cDNA.";
RL   FEBS Lett. 223:169-173(1987).
CC   -!- FUNCTION: This protein participates in processes of transmission
CC       and amplification of the visual signal.
CC   -!- CATALYTIC ACTIVITY: Guanosine 3',5'-cyclic phosphate + H(2)O =
CC       guanosine 5'-phosphate.
CC   -!- SUBUNIT: Oligomer composed of two catalytic chains (alpha and
CC       beta), an inhibitory chain (gamma) and the delta chain.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic
CC       side (Potential).
CC   -!- SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase
CC       family.
CC   -!- SIMILARITY: Contains 2 GAF domains.
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DR   EMBL; X12756; CAA31243.1; -; mRNA.
DR   EMBL; M27541; AAA30441.1; -; mRNA.
DR   EMBL; M36683; AAA30442.1; -; mRNA.
DR   EMBL; M26043; AAA30443.1; -; mRNA.
DR   IPI; IPI00690805; -.
DR   PIR; S06418; S06418.
DR   RefSeq; NP_001001526.2; -.
DR   UniGene; Bt.4147; -.
DR   HSSP; Q08499; 1OYN.
DR   Ensembl; ENSBTAG00000012945; Bos taurus.
DR   GeneID; 281973; -.
DR   KEGG; bta:281973; -.
DR   HOVERGEN; P11541; -.
DR   BRENDA; 3.1.4.35; 251.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0047555; F:3',5'-cyclic-GMP phosphodiesterase activity; IEA:EC.
DR   GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR003607; Met-dep_phosphohydro_HD.
DR   InterPro; IPR002073; PDEase.
DR   Pfam; PF01590; GAF; 2.
DR   Pfam; PF00233; PDEase_I; 1.
DR   PRINTS; PR00387; PDIESTERASE1.
DR   SMART; SM00065; GAF; 2.
DR   SMART; SM00471; HDc; 1.
DR   PROSITE; PS00126; PDEASE_I; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cell membrane; cGMP; Direct protein sequencing;
KW   Hydrolase; Lipoprotein; Membrane; Prenylation; Repeat;
KW   Sensory transduction; Vision.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    859       Rod cGMP-specific 3',5'-cyclic
FT                                phosphodiesterase subunit alpha.
FT                                /FTId=PRO_0000198826.
FT   DOMAIN       73    222       GAF 1.
FT   DOMAIN      254    431       GAF 2.
FT   MOD_RES       2      2       N-acetylglycine.
FT   LIPID       856    856       S-farnesyl cysteine (By similarity).
FT   VARIANT     381    381       M -> V.
FT   CONFLICT    194    194       V -> A (in Ref. 2 and 3).
FT   CONFLICT    424    424       T -> A (in Ref. 2 and 3).
FT   CONFLICT    675    675       F -> C (in Ref. 2 and 3).
SQ   SEQUENCE   859 AA;  99341 MW;  701CB148F73A1303 CRC64;
     MGEVTAEEVE KFLDSNVSFA KQYYNLRYRA KVISDLLGPR EAAVDFSNYH ALNSVEESEI
     IFDLLRDFQD NLQAEKCVFN VMKKLCFLLQ ADRMSLFMYR ARNGIAELAT RLFNVHKDAV
     LEECLVAPDS EIVFPLDMGV VGHVALSKKI VNVPNTEEDE HFCDFVDTLT EYQTKNILAS
     PIMNGKDVVA IIMVVNKVDG PHFTENDEEI LLKYLNFANL IMKVFHLSYL HNCETRRGQI
     LLWSGSKVFE ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP
     PYAGPRTPDG REINFYKVID YILHGKEDIK VIPNPPPDHW ALVSGLPTYV AQNGLICNIM
     NAPSEDFFAF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV ATFYNRKDGK PFDEMDETLM
     ESLTQFLGWS VLNPDTYELM NKLENRKDIF QDMVKYHVKC DNEEIQTILK TREVYGKEPW
     ECEEEELAEI LQGELPDADK YEINKFHFSD LPLTELELVK CGIQMYYELK VVDKFHIPQE
     ALVRFMYSLS KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD
     IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL NRRQHEHAIH
     MMDIAIIATD LALYFKKRTM FQKIVDQSKT YETQQEWTQY MMLDQTRKEI VMAMMMTACD
     LSAITKPWEV QSKVALLVAA EFWEQGDLER TVLQQNPIPM MDRNKADELP KLQVGFIDFV
     CTFVYKEFSR FHEEITPMLD GITNNRKEWK ALADEYETKM KGLEEEKQKQ QAANQAAAGS
     QHGGKQPGGG PASKSCCVQ
//