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Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Gene

PDE6A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein participates in processes of transmission and amplification of the visual signal.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei559Proton donorBy similarity1
Metal bindingi563Divalent metal cation 1By similarity1
Metal bindingi599Divalent metal cation 1By similarity1
Metal bindingi600Divalent metal cation 1By similarity1
Metal bindingi600Divalent metal cation 2By similarity1
Metal bindingi720Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processSensory transduction, Vision
LigandcGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (EC:3.1.4.35)
Short name:
GMP-PDE alpha
Alternative name(s):
PDE V-B1
Gene namesi
Name:PDE6A
Synonyms:PDEA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3741.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001988262 – 856Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alphaAdd BLAST855
PropeptideiPRO_0000396695857 – 859Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine1 Publication1
Modified residuei856Cysteine methyl esterBy similarity1
Lipidationi856S-farnesyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP11541.
PRIDEiP11541.

PTM databases

iPTMnetiP11541.

Interactioni

Subunit structurei

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Protein-protein interaction databases

IntActiP11541. 2 interactors.
MINTiMINT-6824874.
STRINGi9913.ENSBTAP00000036518.

Chemistry databases

BindingDBiP11541.

Structurei

3D structure databases

ProteinModelPortaliP11541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 222GAF 1Add BLAST150
Domaini254 – 431GAF 2Add BLAST178

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3689. Eukaryota.
ENOG410XRI7. LUCA.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiP11541.
KOiK08718.

Family and domain databases

CDDicd00077. HDc. 1 hit.
Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProiView protein in InterPro
IPR003018. GAF.
IPR029016. GAF-like_dom_sf.
IPR003607. HD/PDEase_dom.
IPR032958. PDE6A.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR036971. PDEase_catalytic_dom_sf.
IPR023174. PDEase_CS.
PANTHERiPTHR11347:SF115. PTHR11347:SF115. 1 hit.
PfamiView protein in Pfam
PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
PRINTSiPR00387. PDIESTERASE1.
SMARTiView protein in SMART
SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiView protein in PROSITE
PS00126. PDEASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEVTAEEVE KFLDSNVSFA KQYYNLRYRA KVISDLLGPR EAAVDFSNYH
60 70 80 90 100
ALNSVEESEI IFDLLRDFQD NLQAEKCVFN VMKKLCFLLQ ADRMSLFMYR
110 120 130 140 150
ARNGIAELAT RLFNVHKDAV LEECLVAPDS EIVFPLDMGV VGHVALSKKI
160 170 180 190 200
VNVPNTEEDE HFCDFVDTLT EYQTKNILAS PIMNGKDVVA IIMVVNKVDG
210 220 230 240 250
PHFTENDEEI LLKYLNFANL IMKVFHLSYL HNCETRRGQI LLWSGSKVFE
260 270 280 290 300
ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP
310 320 330 340 350
PYAGPRTPDG REINFYKVID YILHGKEDIK VIPNPPPDHW ALVSGLPTYV
360 370 380 390 400
AQNGLICNIM NAPSEDFFAF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV
410 420 430 440 450
ATFYNRKDGK PFDEMDETLM ESLTQFLGWS VLNPDTYELM NKLENRKDIF
460 470 480 490 500
QDMVKYHVKC DNEEIQTILK TREVYGKEPW ECEEEELAEI LQGELPDADK
510 520 530 540 550
YEINKFHFSD LPLTELELVK CGIQMYYELK VVDKFHIPQE ALVRFMYSLS
560 570 580 590 600
KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD
610 620 630 640 650
IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL
660 670 680 690 700
NRRQHEHAIH MMDIAIIATD LALYFKKRTM FQKIVDQSKT YETQQEWTQY
710 720 730 740 750
MMLDQTRKEI VMAMMMTACD LSAITKPWEV QSKVALLVAA EFWEQGDLER
760 770 780 790 800
TVLQQNPIPM MDRNKADELP KLQVGFIDFV CTFVYKEFSR FHEEITPMLD
810 820 830 840 850
GITNNRKEWK ALADEYETKM KGLEEEKQKQ QAANQAAAGS QHGGKQPGGG

PASKSCCVQ
Length:859
Mass (Da):99,341
Last modified:January 23, 2007 - v3
Checksum:i701CB148F73A1303
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194V → A (PubMed:2826095).Curated1
Sequence conflicti194V → A (PubMed:2822478).Curated1
Sequence conflicti424T → A (PubMed:2826095).Curated1
Sequence conflicti424T → A (PubMed:2822478).Curated1
Sequence conflicti675F → C (PubMed:2826095).Curated1
Sequence conflicti675F → C (PubMed:2822478).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti381M → V. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12756 mRNA. Translation: CAA31243.1.
M27541 mRNA. Translation: AAA30441.1.
M36683 mRNA. Translation: AAA30442.1.
M26043 mRNA. Translation: AAA30443.1.
PIRiS06418.
RefSeqiNP_001001526.2. NM_001001526.2.
UniGeneiBt.4147.

Genome annotation databases

GeneIDi281973.
KEGGibta:281973.

Similar proteinsi

Entry informationi

Entry nameiPDE6A_BOVIN
AccessioniPrimary (citable) accession number: P11541
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families