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P11541

- PDE6A_BOVIN

UniProt

P11541 - PDE6A_BOVIN

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Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Gene

PDE6A

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This protein participates in processes of transmission and amplification of the visual signal.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei559 – 5591Proton donorBy similarity
Metal bindingi563 – 5631Divalent metal cation 1By similarity
Metal bindingi599 – 5991Divalent metal cation 1By similarity
Metal bindingi600 – 6001Divalent metal cation 1By similarity
Metal bindingi600 – 6001Divalent metal cation 2By similarity
Metal bindingi720 – 7201Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. signal transduction Source: InterPro
  2. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_211562. Activation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (EC:3.1.4.35)
Short name:
GMP-PDE alpha
Alternative name(s):
PDE V-B1
Gene namesi
Name:PDE6A
Synonyms:PDEA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. photoreceptor disc membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 856855Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alphaPRO_0000198826Add
BLAST
Propeptidei857 – 8593Removed in mature formBy similarityPRO_0000396695

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication
Modified residuei856 – 8561Cysteine methyl esterBy similarity
Lipidationi856 – 8561S-farnesyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

PRIDEiP11541.

Interactioni

Subunit structurei

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Protein-protein interaction databases

IntActiP11541. 2 interactions.
MINTiMINT-6824874.
STRINGi9913.ENSBTAP00000017199.

Structurei

3D structure databases

ProteinModelPortaliP11541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 222150GAF 1Add
BLAST
Domaini254 – 431178GAF 2Add
BLAST

Sequence similaritiesi

Contains 2 GAF domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG242608.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiP11541.
KOiK08718.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11541-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGEVTAEEVE KFLDSNVSFA KQYYNLRYRA KVISDLLGPR EAAVDFSNYH
60 70 80 90 100
ALNSVEESEI IFDLLRDFQD NLQAEKCVFN VMKKLCFLLQ ADRMSLFMYR
110 120 130 140 150
ARNGIAELAT RLFNVHKDAV LEECLVAPDS EIVFPLDMGV VGHVALSKKI
160 170 180 190 200
VNVPNTEEDE HFCDFVDTLT EYQTKNILAS PIMNGKDVVA IIMVVNKVDG
210 220 230 240 250
PHFTENDEEI LLKYLNFANL IMKVFHLSYL HNCETRRGQI LLWSGSKVFE
260 270 280 290 300
ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP
310 320 330 340 350
PYAGPRTPDG REINFYKVID YILHGKEDIK VIPNPPPDHW ALVSGLPTYV
360 370 380 390 400
AQNGLICNIM NAPSEDFFAF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV
410 420 430 440 450
ATFYNRKDGK PFDEMDETLM ESLTQFLGWS VLNPDTYELM NKLENRKDIF
460 470 480 490 500
QDMVKYHVKC DNEEIQTILK TREVYGKEPW ECEEEELAEI LQGELPDADK
510 520 530 540 550
YEINKFHFSD LPLTELELVK CGIQMYYELK VVDKFHIPQE ALVRFMYSLS
560 570 580 590 600
KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD
610 620 630 640 650
IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL
660 670 680 690 700
NRRQHEHAIH MMDIAIIATD LALYFKKRTM FQKIVDQSKT YETQQEWTQY
710 720 730 740 750
MMLDQTRKEI VMAMMMTACD LSAITKPWEV QSKVALLVAA EFWEQGDLER
760 770 780 790 800
TVLQQNPIPM MDRNKADELP KLQVGFIDFV CTFVYKEFSR FHEEITPMLD
810 820 830 840 850
GITNNRKEWK ALADEYETKM KGLEEEKQKQ QAANQAAAGS QHGGKQPGGG

PASKSCCVQ
Length:859
Mass (Da):99,341
Last modified:January 23, 2007 - v3
Checksum:i701CB148F73A1303
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941V → A(PubMed:2826095)Curated
Sequence conflicti194 – 1941V → A(PubMed:2822478)Curated
Sequence conflicti424 – 4241T → A(PubMed:2826095)Curated
Sequence conflicti424 – 4241T → A(PubMed:2822478)Curated
Sequence conflicti675 – 6751F → C(PubMed:2826095)Curated
Sequence conflicti675 – 6751F → C(PubMed:2822478)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti381 – 3811M → V.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12756 mRNA. Translation: CAA31243.1.
M27541 mRNA. Translation: AAA30441.1.
M36683 mRNA. Translation: AAA30442.1.
M26043 mRNA. Translation: AAA30443.1.
PIRiS06418.
RefSeqiNP_001001526.2. NM_001001526.2.
UniGeneiBt.4147.

Genome annotation databases

GeneIDi281973.
KEGGibta:281973.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12756 mRNA. Translation: CAA31243.1 .
M27541 mRNA. Translation: AAA30441.1 .
M36683 mRNA. Translation: AAA30442.1 .
M26043 mRNA. Translation: AAA30443.1 .
PIRi S06418.
RefSeqi NP_001001526.2. NM_001001526.2.
UniGenei Bt.4147.

3D structure databases

ProteinModelPortali P11541.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P11541. 2 interactions.
MINTi MINT-6824874.
STRINGi 9913.ENSBTAP00000017199.

Chemistry

BindingDBi P11541.
ChEMBLi CHEMBL3741.

Proteomic databases

PRIDEi P11541.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281973.
KEGGi bta:281973.

Organism-specific databases

CTDi 5145.

Phylogenomic databases

eggNOGi NOG242608.
HOGENOMi HOG000007069.
HOVERGENi HBG053539.
InParanoidi P11541.
KOi K08718.

Enzyme and pathway databases

Reactomei REACT_211562. Activation of the phototransduction cascade.

Miscellaneous databases

NextBioi 20805844.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 3 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterase alpha-subunit and chromosomal localization of the human gene."
    Pittler S.J., Baehr W., Wasmuth J.J., McConnell D.G., Champagne M.S., VanTuinen P., Ledbetter D., Davis R.L.
    Genomics 6:272-283(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cyclic GMP phosphodiesterase from the bovine retina. Amino acid sequence of the alpha-subunit and nucleotide sequence of corresponding cDNA."
    Yu A., Ovchinnikov A., Gubanov V.V., Khramtsov N.V., Akhmedov N.B., Ishchenko K.A., Zagranichnyi V.E., Vasilevskaya I.A., Rakitina T.V., Atabekova N.V., Barinov A.A., Muradov K.G., Shuvaeva T.M., Bystrov N.S., Severtsova I.V., Lipkin V.M.
    Dokl. Akad. Nauk SSSR 296:487-491(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  3. "Cyclic GMP phosphodiesterase from bovine retina. Amino acid sequence of the alpha-subunit and nucleotide sequence of the corresponding cDNA."
    Ovchinnikov Y.A., Gubanov V.V., Khramtsov N.V., Ischenko K.A., Zagranichny V.E., Muradov K.G., Shuvaeva T.M., Lipkin V.M.
    FEBS Lett. 223:169-173(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACETYLATION AT GLY-2.
    Tissue: Retina.

Entry informationi

Entry nameiPDE6A_BOVIN
AccessioniPrimary (citable) accession number: P11541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3