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P11541

- PDE6A_BOVIN

UniProt

P11541 - PDE6A_BOVIN

Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Gene

PDE6A

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    This protein participates in processes of transmission and amplification of the visual signal.

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei559 – 5591Proton donorBy similarity
    Metal bindingi563 – 5631Divalent metal cation 1By similarity
    Metal bindingi599 – 5991Divalent metal cation 1By similarity
    Metal bindingi600 – 6001Divalent metal cation 1By similarity
    Metal bindingi600 – 6001Divalent metal cation 2By similarity
    Metal bindingi720 – 7201Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. signal transduction Source: InterPro
    2. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_211562. Activation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (EC:3.1.4.35)
    Short name:
    GMP-PDE alpha
    Alternative name(s):
    PDE V-B1
    Gene namesi
    Name:PDE6A
    Synonyms:PDEA
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. photoreceptor disc membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 856855Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alphaPRO_0000198826Add
    BLAST
    Propeptidei857 – 8593Removed in mature formBy similarityPRO_0000396695

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycine1 Publication
    Modified residuei856 – 8561Cysteine methyl esterBy similarity
    Lipidationi856 – 8561S-farnesyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PRIDEiP11541.

    Interactioni

    Subunit structurei

    Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

    Protein-protein interaction databases

    IntActiP11541. 2 interactions.
    MINTiMINT-6824874.
    STRINGi9913.ENSBTAP00000017199.

    Structurei

    3D structure databases

    ProteinModelPortaliP11541.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 222150GAF 1Add
    BLAST
    Domaini254 – 431178GAF 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG242608.
    HOGENOMiHOG000007069.
    HOVERGENiHBG053539.
    InParanoidiP11541.
    KOiK08718.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11541-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEVTAEEVE KFLDSNVSFA KQYYNLRYRA KVISDLLGPR EAAVDFSNYH    50
    ALNSVEESEI IFDLLRDFQD NLQAEKCVFN VMKKLCFLLQ ADRMSLFMYR 100
    ARNGIAELAT RLFNVHKDAV LEECLVAPDS EIVFPLDMGV VGHVALSKKI 150
    VNVPNTEEDE HFCDFVDTLT EYQTKNILAS PIMNGKDVVA IIMVVNKVDG 200
    PHFTENDEEI LLKYLNFANL IMKVFHLSYL HNCETRRGQI LLWSGSKVFE 250
    ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP 300
    PYAGPRTPDG REINFYKVID YILHGKEDIK VIPNPPPDHW ALVSGLPTYV 350
    AQNGLICNIM NAPSEDFFAF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV 400
    ATFYNRKDGK PFDEMDETLM ESLTQFLGWS VLNPDTYELM NKLENRKDIF 450
    QDMVKYHVKC DNEEIQTILK TREVYGKEPW ECEEEELAEI LQGELPDADK 500
    YEINKFHFSD LPLTELELVK CGIQMYYELK VVDKFHIPQE ALVRFMYSLS 550
    KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD 600
    IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL 650
    NRRQHEHAIH MMDIAIIATD LALYFKKRTM FQKIVDQSKT YETQQEWTQY 700
    MMLDQTRKEI VMAMMMTACD LSAITKPWEV QSKVALLVAA EFWEQGDLER 750
    TVLQQNPIPM MDRNKADELP KLQVGFIDFV CTFVYKEFSR FHEEITPMLD 800
    GITNNRKEWK ALADEYETKM KGLEEEKQKQ QAANQAAAGS QHGGKQPGGG 850
    PASKSCCVQ 859
    Length:859
    Mass (Da):99,341
    Last modified:January 23, 2007 - v3
    Checksum:i701CB148F73A1303
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti194 – 1941V → A(PubMed:2826095)Curated
    Sequence conflicti194 – 1941V → A(PubMed:2822478)Curated
    Sequence conflicti424 – 4241T → A(PubMed:2826095)Curated
    Sequence conflicti424 – 4241T → A(PubMed:2822478)Curated
    Sequence conflicti675 – 6751F → C(PubMed:2826095)Curated
    Sequence conflicti675 – 6751F → C(PubMed:2822478)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti381 – 3811M → V.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12756 mRNA. Translation: CAA31243.1.
    M27541 mRNA. Translation: AAA30441.1.
    M36683 mRNA. Translation: AAA30442.1.
    M26043 mRNA. Translation: AAA30443.1.
    PIRiS06418.
    RefSeqiNP_001001526.2. NM_001001526.2.
    UniGeneiBt.4147.

    Genome annotation databases

    GeneIDi281973.
    KEGGibta:281973.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12756 mRNA. Translation: CAA31243.1 .
    M27541 mRNA. Translation: AAA30441.1 .
    M36683 mRNA. Translation: AAA30442.1 .
    M26043 mRNA. Translation: AAA30443.1 .
    PIRi S06418.
    RefSeqi NP_001001526.2. NM_001001526.2.
    UniGenei Bt.4147.

    3D structure databases

    ProteinModelPortali P11541.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P11541. 2 interactions.
    MINTi MINT-6824874.
    STRINGi 9913.ENSBTAP00000017199.

    Chemistry

    BindingDBi P11541.
    ChEMBLi CHEMBL3741.

    Proteomic databases

    PRIDEi P11541.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281973.
    KEGGi bta:281973.

    Organism-specific databases

    CTDi 5145.

    Phylogenomic databases

    eggNOGi NOG242608.
    HOGENOMi HOG000007069.
    HOVERGENi HBG053539.
    InParanoidi P11541.
    KOi K08718.

    Enzyme and pathway databases

    Reactomei REACT_211562. Activation of the phototransduction cascade.

    Miscellaneous databases

    NextBioi 20805844.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterase alpha-subunit and chromosomal localization of the human gene."
      Pittler S.J., Baehr W., Wasmuth J.J., McConnell D.G., Champagne M.S., VanTuinen P., Ledbetter D., Davis R.L.
      Genomics 6:272-283(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cyclic GMP phosphodiesterase from the bovine retina. Amino acid sequence of the alpha-subunit and nucleotide sequence of corresponding cDNA."
      Yu A., Ovchinnikov A., Gubanov V.V., Khramtsov N.V., Akhmedov N.B., Ishchenko K.A., Zagranichnyi V.E., Vasilevskaya I.A., Rakitina T.V., Atabekova N.V., Barinov A.A., Muradov K.G., Shuvaeva T.M., Bystrov N.S., Severtsova I.V., Lipkin V.M.
      Dokl. Akad. Nauk SSSR 296:487-491(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    3. "Cyclic GMP phosphodiesterase from bovine retina. Amino acid sequence of the alpha-subunit and nucleotide sequence of the corresponding cDNA."
      Ovchinnikov Y.A., Gubanov V.V., Khramtsov N.V., Ischenko K.A., Zagranichny V.E., Muradov K.G., Shuvaeva T.M., Lipkin V.M.
      FEBS Lett. 223:169-173(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACETYLATION AT GLY-2.
      Tissue: Retina.

    Entry informationi

    Entry nameiPDE6A_BOVIN
    AccessioniPrimary (citable) accession number: P11541
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3