Reviewed, UniProtKB/Swiss-Prot P11541 (PDE6A_BOVIN)

Names and origin

Protein names

Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
      Short name=GMP-PDE alpha
    EC=3.1.4.35
Alternative name(s):
    PDE V-B1

Gene names

Name:	PDE6A
Synonyms:	PDEA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein participates in processes of transmission and amplification of the visual signal.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Subunit structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Acetylation Lipoprotein Prenylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 859	858	Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha		PRO_0000198826
Regions
	Domain	73 – 222	150	GAF 1
	Domain	254 – 431	178	GAF 2
Amino acid modifications
	Modified residue	2	1	N-acetylglycine Ref.3
	Lipidation	856	1	S-farnesyl cysteine By similarity
Natural variations
	Natural variant	381	1	M → V
Experimental info
	Sequence conflict	194	1	V → A Ref.2
	Sequence conflict	194	1	V → A Ref.3
	Sequence conflict	424	1	T → A Ref.2
	Sequence conflict	424	1	T → A Ref.3
	Sequence conflict	675	1	F → C Ref.2
	Sequence conflict	675	1	F → C Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P11541-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 701CB148F73A1303	FASTA	859	99,341
	10 20 30 40 50 60 MGEVTAEEVE KFLDSNVSFA KQYYNLRYRA KVISDLLGPR EAAVDFSNYH ALNSVEESEI 70 80 90 100 110 120 IFDLLRDFQD NLQAEKCVFN VMKKLCFLLQ ADRMSLFMYR ARNGIAELAT RLFNVHKDAV 130 140 150 160 170 180 LEECLVAPDS EIVFPLDMGV VGHVALSKKI VNVPNTEEDE HFCDFVDTLT EYQTKNILAS 190 200 210 220 230 240 PIMNGKDVVA IIMVVNKVDG PHFTENDEEI LLKYLNFANL IMKVFHLSYL HNCETRRGQI 250 260 270 280 290 300 LLWSGSKVFE ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP 310 320 330 340 350 360 PYAGPRTPDG REINFYKVID YILHGKEDIK VIPNPPPDHW ALVSGLPTYV AQNGLICNIM 370 380 390 400 410 420 NAPSEDFFAF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV ATFYNRKDGK PFDEMDETLM 430 440 450 460 470 480 ESLTQFLGWS VLNPDTYELM NKLENRKDIF QDMVKYHVKC DNEEIQTILK TREVYGKEPW 490 500 510 520 530 540 ECEEEELAEI LQGELPDADK YEINKFHFSD LPLTELELVK CGIQMYYELK VVDKFHIPQE 550 560 570 580 590 600 ALVRFMYSLS KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD 610 620 630 640 650 660 IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL NRRQHEHAIH 670 680 690 700 710 720 MMDIAIIATD LALYFKKRTM FQKIVDQSKT YETQQEWTQY MMLDQTRKEI VMAMMMTACD 730 740 750 760 770 780 LSAITKPWEV QSKVALLVAA EFWEQGDLER TVLQQNPIPM MDRNKADELP KLQVGFIDFV 790 800 810 820 830 840 CTFVYKEFSR FHEEITPMLD GITNNRKEWK ALADEYETKM KGLEEEKQKQ QAANQAAAGS 850 QHGGKQPGGG PASKSCCVQ

References

[1]	"Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterase alpha-subunit and chromosomal localization of the human gene." Pittler S.J., Baehr W., Wasmuth J.J., McConnell D.G., Champagne M.S., VanTuinen P., Ledbetter D., Davis R.L. Genomics 6:272-283(1990) [PubMed: 2155175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cyclic GMP phosphodiesterase from the bovine retina. Amino acid sequence of the alpha-subunit and nucleotide sequence of corresponding cDNA." Yu A., Ovchinnikov A., Gubanov V.V., Khramtsov N.V., Akhmedov N.B., Ishchenko K.A., Zagranichnyi V.E., Vasilevskaya I.A., Rakitina T.V., Atabekova N.V., Barinov A.A., Muradov K.G., Shuvaeva T.M., Bystrov N.S., Severtsova I.V., Lipkin V.M. Dokl. Akad. Nauk SSSR 296:487-491(1987) [PubMed: 2826095] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[3]	"Cyclic GMP phosphodiesterase from bovine retina. Amino acid sequence of the alpha-subunit and nucleotide sequence of the corresponding cDNA." Ovchinnikov Y.A., Gubanov V.V., Khramtsov N.V., Ischenko K.A., Zagranichny V.E., Muradov K.G., Shuvaeva T.M., Lipkin V.M. FEBS Lett. 223:169-173(1987) [PubMed: 2822478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACETYLATION AT GLY-2. Tissue: Retina.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X12756 mRNA. Translation: CAA31243.1. M27541 mRNA. Translation: AAA30441.1. M36683 mRNA. Translation: AAA30442.1. M26043 mRNA. Translation: AAA30443.1.
IPI	IPI00690805.
PIR	S06418.
RefSeq	NP_001001526.2.
UniGene	Bt.4147
3D structure databases
HSSP	HSSP built from PDB template 1OYN based on UniProtKB Q08499.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000012945. Bos taurus. [Contig view]
GeneID	281973.
KEGG	bta:281973.
Phylogenomic databases
HOVERGEN	P11541.
Enzyme and pathway databases
BRENDA	3.1.4.35. 251.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6A_BOVIN

Accession

Primary (citable) accession number: P11541

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)