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Protein

Cytochrome P450 2C12, female-specific

Gene

Cyp2c12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This P450 is active in 15-beta-hydroxylation of steroid sulfates.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi435 – 4351Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C12, female-specific (EC:1.14.14.1)
Alternative name(s):
CYPIIC12
Cytochrome P450 15-beta
Cytochrome P450-UT-1
Cytochrome P450-UT-I
Cytochrome P450I
Gene namesi
Name:Cyp2c12
Synonyms:Cyp2c-12, Cyp2c40
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2470. Cyp2c12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Cytochrome P450 2C12, female-specificPRO_0000051702Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei249 – 2491N6-acetyllysineBy similarity
Modified residuei375 – 3751N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP11510.

Expressioni

Inductioni

By growth hormone.

Gene expression databases

GenevisibleiP11510. RN.

Interactioni

Protein-protein interaction databases

IntActiP11510. 1 interaction.
MINTiMINT-4577921.
STRINGi10116.ENSRNOP00000015802.

Structurei

3D structure databases

ProteinModelPortaliP11510.
SMRiP11510. Positions 30-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG015789.
InParanoidiP11510.
KOiK07413.
PhylomeDBiP11510.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPFVVLVLS LSFLLLLYLW RPSPGRGKLP PGPTPLPIFG NFLQIDMKDI
60 70 80 90 100
RQSISNFSKT YGPVFTLYFG SQPTVVLHGY EAVKEALIDY GEEFSGRGRM
110 120 130 140 150
PVFEKATKGL GISFSRGNVW RATRHFTVNT LRSLGMGKRT IEIKVQEEAE
160 170 180 190 200
WLVMELKKTK GSPCDPKFII GCAPCNVICS IIFQNRFDYK DKDFLSLIEN
210 220 230 240 250
VNEYIKIVST PAFQVFNAFP ILLDYCPGNH KTHSKHFAAI KSYLLKKIKE
260 270 280 290 300
HEESLDVSNP RDFIDYFLIQ RCQENGNQQM NYTQEHLAIL VTNLFIGGTE
310 320 330 340 350
TSSLTLRFAL LLLMKYPHIT DKVQEEIGQV IGRHRSPCML DRIHMPYTNA
360 370 380 390 400
MIHEVQRYID LAPNGLLHEV TCDTKFRDYF IPKGTAVLTS LTSVLHARKE
410 420 430 440 450
FPNPEMFDPG HFLDENGNFK KSDYFMPFSA GKRKCVGEGL ASMELFLFLT
460 470 480 490
TILQNFKLKS LSDPKDIDIN SIRSEFSSIP PTFQLCFIPV
Length:490
Mass (Da):55,919
Last modified:October 1, 1989 - v1
Checksum:iC2B637DA69F62C82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03786 mRNA. Translation: AAA41005.1.
PIRiA32140.
RefSeqiNP_113760.1. NM_031572.1.
UniGeneiRn.2586.

Genome annotation databases

GeneIDi25011.
KEGGirno:25011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03786 mRNA. Translation: AAA41005.1.
PIRiA32140.
RefSeqiNP_113760.1. NM_031572.1.
UniGeneiRn.2586.

3D structure databases

ProteinModelPortaliP11510.
SMRiP11510. Positions 30-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP11510. 1 interaction.
MINTiMINT-4577921.
STRINGi10116.ENSRNOP00000015802.

Proteomic databases

PRIDEiP11510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25011.
KEGGirno:25011.

Organism-specific databases

CTDi25011.
RGDi2470. Cyp2c12.

Phylogenomic databases

HOVERGENiHBG015789.
InParanoidiP11510.
KOiK07413.
PhylomeDBiP11510.

Miscellaneous databases

NextBioi605089.
PROiP11510.

Gene expression databases

GenevisibleiP11510. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning, sequence, and regulation of a major female-specific and growth hormone-inducible rat liver cytochrome P-450 active in 15 beta-hydroxylation of steroid sulfates."
    Zaphiropoulos P.G., Mode A., Stroem A., Moeller C., Fernandez C., Gustafsson J.-A.
    Proc. Natl. Acad. Sci. U.S.A. 85:4214-4217(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and regulation of two growth-hormone-controlled, sex-specific isozymes of cytochrome P-450 in rat liver, P-450(15)beta and P-450(16)alpha."
    Zaphiropoulos P.G., Mode A., Stroem A., Husman B., Andersson G., Gustafsson J.-A.
    Acta Med. Scand. Suppl. 723:161-167(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCP2CC_RAT
AccessioniPrimary (citable) accession number: P11510
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 24, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.