P11510 (CP2CC_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C12, female-specific
EC=1.14.14.1

Alternative name(s):
CYPIIC12
Cytochrome P450 15-beta
Cytochrome P450-UT-1
Cytochrome P450-UT-I
Cytochrome P450I

Gene names

Name:	Cyp2c12
Synonyms:	Cyp2c-12, Cyp2c40

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This P450 is active in 15-beta-hydroxylation of steroid sulfates.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By growth hormone.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 2C12, female-specific		PRO_0000051702
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11510 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: C2B637DA69F62C82	FASTA	490	55,919
	10 20 30 40 50 60 MDPFVVLVLS LSFLLLLYLW RPSPGRGKLP PGPTPLPIFG NFLQIDMKDI RQSISNFSKT 70 80 90 100 110 120 YGPVFTLYFG SQPTVVLHGY EAVKEALIDY GEEFSGRGRM PVFEKATKGL GISFSRGNVW 130 140 150 160 170 180 RATRHFTVNT LRSLGMGKRT IEIKVQEEAE WLVMELKKTK GSPCDPKFII GCAPCNVICS 190 200 210 220 230 240 IIFQNRFDYK DKDFLSLIEN VNEYIKIVST PAFQVFNAFP ILLDYCPGNH KTHSKHFAAI 250 260 270 280 290 300 KSYLLKKIKE HEESLDVSNP RDFIDYFLIQ RCQENGNQQM NYTQEHLAIL VTNLFIGGTE 310 320 330 340 350 360 TSSLTLRFAL LLLMKYPHIT DKVQEEIGQV IGRHRSPCML DRIHMPYTNA MIHEVQRYID 370 380 390 400 410 420 LAPNGLLHEV TCDTKFRDYF IPKGTAVLTS LTSVLHARKE FPNPEMFDPG HFLDENGNFK 430 440 450 460 470 480 KSDYFMPFSA GKRKCVGEGL ASMELFLFLT TILQNFKLKS LSDPKDIDIN SIRSEFSSIP 490 PTFQLCFIPV

References

[1]	"cDNA cloning, sequence, and regulation of a major female-specific and growth hormone-inducible rat liver cytochrome P-450 active in 15 beta-hydroxylation of steroid sulfates." Zaphiropoulos P.G., Mode A., Stroem A., Moeller C., Fernandez C., Gustafsson J.-A. Proc. Natl. Acad. Sci. U.S.A. 85:4214-4217(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and regulation of two growth-hormone-controlled, sex-specific isozymes of cytochrome P-450 in rat liver, P-450(15)beta and P-450(16)alpha." Zaphiropoulos P.G., Mode A., Stroem A., Husman B., Andersson G., Gustafsson J.-A. Acta Med. Scand. Suppl. 723:161-167(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03786 mRNA. Translation: AAA41005.1.
IPI	IPI00324707.
PIR	A32140.
RefSeq	NP_113760.1. NM_031572.1.
UniGene	Rn.2586.
3D structure databases
ProteinModelPortal	P11510.
SMR	P11510. Positions 30-490.
ModBase	Search...
Protein-protein interaction databases
IntAct	P11510. 1 interaction.
MINT	MINT-4577921.
Proteomic databases
PRIDE	P11510.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25011.
KEGG	rno:25011.
Organism-specific databases
CTD	25011.
RGD	2470. Cyp2c12.
Phylogenomic databases
HOVERGEN	HBG015789.
KO	K07413.
Gene expression databases
Genevestigator	P11510.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605089.

Entry information

Entry name

CP2CC_RAT

Accession

Primary (citable) accession number: P11510

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program