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P11509

- CP2A6_HUMAN

UniProt

P11509 - CP2A6_HUMAN

Protein

Cytochrome P450 2A6

Gene

CYP2A6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 174 (01 Oct 2014)
      Sequence version 3 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity.6 Publications

    Catalytic activityi

    1,4-cineole + NADPH + O2 = 2-exo-hydroxy-1,4-cineole + NADP+ + H2O.1 Publication

    Cofactori

    Heme group.

    Kineticsi

    1. KM=0.23 µM for coumarin2 Publications
    2. KM=530 µM for 1,4-cineole2 Publications

    Vmax=3.5 nmol/min/nmol enzyme toward 2-exo-hydroxy-1,4-cineole2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei107 – 1071SubstrateCurated
    Binding sitei297 – 2971Substrate
    Metal bindingi439 – 4391Iron (heme axial ligand)

    GO - Molecular functioni

    1. coumarin 7-hydroxylase activity Source: UniProtKB
    2. enzyme binding Source: BHF-UCL
    3. heme binding Source: UniProtKB
    4. iron ion binding Source: InterPro
    5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: InterPro

    GO - Biological processi

    1. coumarin catabolic process Source: BHF-UCL
    2. coumarin metabolic process Source: BHF-UCL
    3. drug metabolic process Source: BHF-UCL
    4. exogenous drug catabolic process Source: BHF-UCL
    5. small molecule metabolic process Source: Reactome
    6. steroid metabolic process Source: BHF-UCL
    7. xenobiotic metabolic process Source: Reactome

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:HS10343-MONOMER.
    ReactomeiREACT_13543. Xenobiotics.
    REACT_13797. CYP2E1 reactions.
    SABIO-RKP11509.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A6 (EC:1.14.13.-)
    Alternative name(s):
    1,4-cineole 2-exo-monooxygenase
    CYPIIA6
    Coumarin 7-hydroxylase
    Cytochrome P450 IIA3
    Cytochrome P450(I)
    Gene namesi
    Name:CYP2A6
    Synonyms:CYP2A3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:2610. CYP2A6.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic microtubule Source: UniProtKB
    2. endoplasmic reticulum membrane Source: Reactome

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi208 – 2081I → S: Increases phenacetin O-deethylation activity 10 fold; when associated with F-300 and A-301. Increases phenacetin O-deethylation activity 38 fold; when associated with F-300; A-301 and G-369. 1 Publication
    Mutagenesisi213 – 2131S → A: No effect on phenacetin O-deethylation activity. 1 Publication
    Mutagenesisi300 – 3001I → F: Increases phenacetin O-deethylation activity 3 fold. Increases phenacetin O-deethylation activity 8 fold; when associated with A-301. Increases phenacetin O-deethylation activity 10 fold; when associated with S-208 and A-301. Increases phenacetin O-deethylation activity 12 fold; when associated with A-301 and G-369. Increases phenacetin O-deethylation activity 38 fold; when associated with S-208; A-301 and G-369. 1 Publication
    Mutagenesisi301 – 3011G → A: Slightly decreases phenacetin O-deethylation activity. Increases phenacetin O-deethylation activity 8 fold; when associated with F-300. Increases phenacetin O-deethylation activity 10 fold; when associated with S-208 and F-300. Increases phenacetin O-deethylation activity 12 fold; when associated with F-300 and G-369. Increases phenacetin O-deethylation activity 38 fold; when associated with S-208; F-300 and G-369. 1 Publication
    Mutagenesisi369 – 3691S → G: Increases phenacetin O-deethylation activity 3 fold. Increases phenacetin O-deethylation activity 38 fold; when associated with S-208; F-300 and A-301. 1 Publication
    Mutagenesisi372 – 3721R → H: Increases phenacetin O-deethylation activity 2 fold. 1 Publication

    Organism-specific databases

    MIMi122720. gene+phenotype.
    PharmGKBiPA121.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Cytochrome P450 2A6PRO_0000051668Add
    BLAST

    Proteomic databases

    PaxDbiP11509.
    PRIDEiP11509.

    PTM databases

    PhosphoSiteiP11509.

    Expressioni

    Tissue specificityi

    Liver.2 Publications

    Inductioni

    By phenobarbital and dexamethasone.2 Publications

    Gene expression databases

    ArrayExpressiP11509.
    BgeeiP11509.
    GenevestigatoriP11509.

    Organism-specific databases

    HPAiHPA046713.
    HPA047262.

    Interactioni

    Protein-protein interaction databases

    BioGridi107927. 2 interactions.

    Structurei

    Secondary structure

    1
    494
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni41 – 433
    Helixi46 – 483
    Helixi51 – 533
    Helixi54 – 6512
    Beta strandi67 – 737
    Beta strandi76 – 816
    Helixi84 – 918
    Turni92 – 987
    Helixi105 – 1117
    Beta strandi115 – 1184
    Helixi121 – 13717
    Turni138 – 1414
    Helixi143 – 16220
    Turni163 – 1653
    Helixi171 – 18717
    Helixi196 – 21217
    Helixi215 – 22713
    Beta strandi230 – 2323
    Helixi233 – 25624
    Helixi267 – 27711
    Turni278 – 2803
    Helixi288 – 31932
    Helixi321 – 33414
    Beta strandi337 – 3393
    Helixi343 – 3486
    Helixi350 – 36314
    Beta strandi378 – 3803
    Beta strandi383 – 3853
    Beta strandi390 – 3934
    Helixi395 – 3995
    Turni402 – 4043
    Helixi413 – 4164
    Beta strandi419 – 4213
    Helixi442 – 45918
    Beta strandi460 – 4667
    Helixi468 – 4703
    Beta strandi476 – 4838
    Beta strandi489 – 4935

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Z10X-ray1.90A/B/C/D29-494[»]
    1Z11X-ray2.05A/B/C/D29-494[»]
    2FDUX-ray1.85A/B/C/D29-494[»]
    2FDVX-ray1.65A/B/C/D29-494[»]
    2FDWX-ray2.05A/B/C/D29-494[»]
    2FDYX-ray1.95A/B/C/D29-494[»]
    3EBSX-ray2.15A/B/C/D29-494[»]
    3T3QX-ray2.10A/B/C/D29-494[»]
    3T3RX-ray2.40A/B/C/D29-494[»]
    4EJJX-ray2.30A/B/C/D29-494[»]
    ProteinModelPortaliP11509.
    SMRiP11509. Positions 30-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11509.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOVERGENiHBG015789.
    InParanoidiP11509.
    KOiK17683.
    OMAiDTHGANI.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP11509.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11509-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLASGMLLVA LLVCLTVMVL MSVWQQRKSK GKLPPGPTPL PFIGNYLQLN    50
    TEQMYNSLMK ISERYGPVFT IHLGPRRVVV LCGHDAVREA LVDQAEEFSG 100
    RGEQATFDWV FKGYGVVFSN GERAKQLRRF SIATLRDFGV GKRGIEERIQ 150
    EEAGFLIDAL RGTGGANIDP TFFLSRTVSN VISSIVFGDR FDYKDKEFLS 200
    LLRMMLGIFQ FTSTSTGQLY EMFSSVMKHL PGPQQQAFQL LQGLEDFIAK 250
    KVEHNQRTLD PNSPRDFIDS FLIRMQEEEK NPNTEFYLKN LVMTTLNLFI 300
    GGTETVSTTL RYGFLLLMKH PEVEAKVHEE IDRVIGKNRQ PKFEDRAKMP 350
    YMEAVIHEIQ RFGDVIPMSL ARRVKKDTKF RDFFLPKGTE VFPMLGSVLR 400
    DPSFFSNPQD FNPQHFLNEK GQFKKSDAFV PFSIGKRNCF GEGLARMELF 450
    LFFTTVMQNF RLKSSQSPKD IDVSPKHVGF ATIPRNYTMS FLPR 494
    Length:494
    Mass (Da):56,501
    Last modified:October 5, 2010 - v3
    Checksum:i8C05CBF0EFC698AF
    GO

    Sequence cautioni

    The sequence AAA52147.1 differs from that shown. Reason: Numerous conflicts and frameshifts.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 75Missing in CAA32097. (PubMed:2726448)Curated
    Sequence conflicti255 – 2551N → K in CAA32097. (PubMed:2726448)Curated
    Sequence conflicti326 – 3261K → Q in CAA32097. (PubMed:2726448)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti5 – 51G → R in allele CYP2A6*13. 2 Publications
    Corresponds to variant rs28399434 [ dbSNP | Ensembl ].
    VAR_018330
    Natural varianti29 – 291S → N in allele CYP2A6*14. 3 Publications
    Corresponds to variant rs28399435 [ dbSNP | Ensembl ].
    VAR_018331
    Natural varianti110 – 1101V → L in allele CYP2A6*24, increases phenacetin O-deethylation activity 4 fold. 1 Publication
    Corresponds to variant rs72549435 [ dbSNP | Ensembl ].
    VAR_055035
    Natural varianti118 – 1181F → L in allele CYP2A6*25 and allele CYP2A6*26. 2 Publications
    Corresponds to variant rs28399440 [ dbSNP | Ensembl ].
    VAR_024711
    Natural varianti128 – 1281R → L in allele CYP2A6*26. 1 Publication
    VAR_055036
    Natural varianti128 – 1281R → Q in allele CYP2A6*6; loss of activity. 2 Publications
    Corresponds to variant rs4986891 [ dbSNP | Ensembl ].
    VAR_011577
    Natural varianti131 – 1311S → A in allele CYP2A6*26. 1 Publication
    Corresponds to variant rs59552350 [ dbSNP | Ensembl ].
    VAR_055037
    Natural varianti160 – 1601L → H in allele CYP2A6*2; unable to catalyze 7-hydroxylation of coumarin; causes switching from coumarin 7-hydroxylation to 3-hydroxylation. 4 Publications
    Corresponds to variant rs1801272 [ dbSNP | Ensembl ].
    VAR_001249
    Natural varianti194 – 1941K → E in allele CYP2A6*15. 1 Publication
    VAR_018332
    Natural varianti203 – 2031R → C in allele CYP2A6*23; greatly reduced activity toward nicotine C-oxidation as well as reduced coumarin 7-hydroxylation. 2 Publications
    Corresponds to variant rs56256500 [ dbSNP | Ensembl ].
    VAR_055034
    Natural varianti203 – 2031R → S in allele CYP2A6*16. 2 Publications
    Corresponds to variant rs56256500 [ dbSNP | Ensembl ].
    VAR_018333
    Natural varianti224 – 2241S → P.1 Publication
    Corresponds to variant rs111033610 [ dbSNP | Ensembl ].
    VAR_024712
    Natural varianti292 – 2921V → M.
    Corresponds to variant rs2644906 [ dbSNP | Ensembl ].
    VAR_059149
    Natural varianti294 – 2941T → S.
    Corresponds to variant rs4997557 [ dbSNP | Ensembl ].
    VAR_048448
    Natural varianti365 – 3651V → M in allele CYP2A6*17, increases phenacetin O-deethylation activity 2 fold. 3 Publications
    Corresponds to variant rs28399454 [ dbSNP | Ensembl ].
    VAR_024713
    Natural varianti392 – 3921F → Y.9 Publications
    Corresponds to variant rs1809810 [ dbSNP | Ensembl ].
    VAR_055033
    Natural varianti418 – 4181N → D in allele CYP2A6*28. 3 Publications
    Corresponds to variant rs28399463 [ dbSNP | Ensembl ].
    VAR_024714
    Natural varianti419 – 4191E → D in allele CYP2A6*28. 4 Publications
    Corresponds to variant rs8192730 [ dbSNP | Ensembl ].
    VAR_018375
    Natural varianti438 – 4381N → Y in allele CYP2A6*24. 1 Publication
    VAR_055038
    Natural varianti471 – 4711I → T in allele CYP2A6*7. 3 Publications
    Corresponds to variant rs5031016 [ dbSNP | Ensembl ].
    VAR_011578
    Natural varianti476 – 4761K → R.2 Publications
    Corresponds to variant rs6413474 [ dbSNP | Ensembl ].
    VAR_024715
    Natural varianti479 – 4791G → V in allele CYP2A6*5; loss of activity. 1 Publication
    Corresponds to variant rs5031017 [ dbSNP | Ensembl ].
    VAR_008356
    Natural varianti485 – 4851R → L in allele CYP2A6*8. 2 Publications
    Corresponds to variant rs28399468 [ dbSNP | Ensembl ].
    VAR_011579

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13897 mRNA. Translation: CAA32097.1.
    X13929 mRNA. Translation: CAA32117.1.
    X13930 mRNA. Translation: CAA32118.1.
    M33318 mRNA. Translation: AAA52067.1.
    AF182275 mRNA. Translation: AAF13600.1.
    AK312964 mRNA. Translation: BAG35803.1.
    EU135979 Genomic DNA. Translation: ABV02584.1.
    FJ440681 Genomic DNA. Translation: ACK44068.1.
    AC008537 Genomic DNA. No translation available.
    CH471126 Genomic DNA. Translation: EAW57012.1.
    BC096253 mRNA. Translation: AAH96253.3.
    BC096254 mRNA. Translation: AAH96254.1.
    BC096255 mRNA. Translation: AAH96255.1.
    BC096256 mRNA. Translation: AAH96256.1.
    AF326721 Genomic DNA. Translation: AAG45229.1.
    K03192 mRNA. Translation: AAA52147.1. Sequence problems.
    CCDSiCCDS12568.1.
    PIRiA00190. O4HUPB.
    S04698. O4HUA6.
    RefSeqiNP_000753.3. NM_000762.5.
    UniGeneiHs.250615.

    Genome annotation databases

    EnsembliENST00000301141; ENSP00000301141; ENSG00000255974.
    GeneIDi1548.
    KEGGihsa:1548.
    UCSCiuc002opl.4. human.

    Polymorphism databases

    DMDMi308153612.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Cytochrome P450 Allele Nomenclature Committee

    CYP2A6 alleles

    NIEHS-SNPs
    Wikipedia

    CYP2A6 entry

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13897 mRNA. Translation: CAA32097.1 .
    X13929 mRNA. Translation: CAA32117.1 .
    X13930 mRNA. Translation: CAA32118.1 .
    M33318 mRNA. Translation: AAA52067.1 .
    AF182275 mRNA. Translation: AAF13600.1 .
    AK312964 mRNA. Translation: BAG35803.1 .
    EU135979 Genomic DNA. Translation: ABV02584.1 .
    FJ440681 Genomic DNA. Translation: ACK44068.1 .
    AC008537 Genomic DNA. No translation available.
    CH471126 Genomic DNA. Translation: EAW57012.1 .
    BC096253 mRNA. Translation: AAH96253.3 .
    BC096254 mRNA. Translation: AAH96254.1 .
    BC096255 mRNA. Translation: AAH96255.1 .
    BC096256 mRNA. Translation: AAH96256.1 .
    AF326721 Genomic DNA. Translation: AAG45229.1 .
    K03192 mRNA. Translation: AAA52147.1 . Sequence problems.
    CCDSi CCDS12568.1.
    PIRi A00190. O4HUPB.
    S04698. O4HUA6.
    RefSeqi NP_000753.3. NM_000762.5.
    UniGenei Hs.250615.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Z10 X-ray 1.90 A/B/C/D 29-494 [» ]
    1Z11 X-ray 2.05 A/B/C/D 29-494 [» ]
    2FDU X-ray 1.85 A/B/C/D 29-494 [» ]
    2FDV X-ray 1.65 A/B/C/D 29-494 [» ]
    2FDW X-ray 2.05 A/B/C/D 29-494 [» ]
    2FDY X-ray 1.95 A/B/C/D 29-494 [» ]
    3EBS X-ray 2.15 A/B/C/D 29-494 [» ]
    3T3Q X-ray 2.10 A/B/C/D 29-494 [» ]
    3T3R X-ray 2.40 A/B/C/D 29-494 [» ]
    4EJJ X-ray 2.30 A/B/C/D 29-494 [» ]
    ProteinModelPortali P11509.
    SMRi P11509. Positions 30-494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107927. 2 interactions.

    Chemistry

    BindingDBi P11509.
    ChEMBLi CHEMBL5282.
    DrugBanki DB00356. Chlorzoxazone.
    DB00255. Diethylstilbestrol.
    DB00783. Estradiol.
    DB00977. Ethinyl Estradiol.
    DB00983. Formoterol.
    DB01159. Halothane.
    DB01006. Letrozole.
    DB00553. Methoxsalen.
    DB01011. Metyrapone.
    DB00184. Nicotine.
    DB01085. Pilocarpine.
    DB01124. Tolbutamide.
    DB00752. Tranylcypromine.

    PTM databases

    PhosphoSitei P11509.

    Polymorphism databases

    DMDMi 308153612.

    Proteomic databases

    PaxDbi P11509.
    PRIDEi P11509.

    Protocols and materials databases

    DNASUi 1548.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000301141 ; ENSP00000301141 ; ENSG00000255974 .
    GeneIDi 1548.
    KEGGi hsa:1548.
    UCSCi uc002opl.4. human.

    Organism-specific databases

    CTDi 1548.
    GeneCardsi GC19M041352.
    HGNCi HGNC:2610. CYP2A6.
    HPAi HPA046713.
    HPA047262.
    MIMi 122720. gene+phenotype.
    neXtProti NX_P11509.
    PharmGKBi PA121.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2124.
    HOVERGENi HBG015789.
    InParanoidi P11509.
    KOi K17683.
    OMAi DTHGANI.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P11509.
    TreeFami TF352043.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS10343-MONOMER.
    Reactomei REACT_13543. Xenobiotics.
    REACT_13797. CYP2E1 reactions.
    SABIO-RK P11509.

    Miscellaneous databases

    ChiTaRSi CYP2A6. human.
    EvolutionaryTracei P11509.
    GeneWikii CYP2A6.
    GenomeRNAii 1548.
    NextBioi 6401.
    PROi P11509.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P11509.
    Bgeei P11509.
    Genevestigatori P11509.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Close linkage of the human cytochrome P450IIA and P450IIB gene subfamilies: implications for the assignment of substrate specificity."
      Miles J.S., Bickmore W., Brook J.D., McLaren A.W., Meehan R., Wolf C.R.
      Nucleic Acids Res. 17:2907-2917(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASN-29 AND TYR-392.
      Tissue: Liver.
    2. "cDNA and deduced amino acid sequences of human P450 IIA3 (CYP2A3)."
      Yamano S., Nagata K., Yamazoe Y., Kato R., Gelboin H.V., Gonzalez F.J.
      Nucleic Acids Res. 17:4888-4888(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS HIS-160 AND TYR-392.
      Tissue: Hepatocyte.
    3. "The CYP2A3 gene product catalyzes coumarin 7-hydroxylation in human liver microsomes."
      Yamano S., Tatsuno J., Gonzalez F.J.
      Biochemistry 29:1322-1329(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS HIS-160 AND TYR-392, CHARACTERIZATION OF VARIANT HIS-160.
      Tissue: Hepatocyte.
    4. "Sequence of a new human cytochrome P450-2A6 cDNA."
      Zhuge J., Qian Y., Xie H., Yu Y.
      Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT TYR-392.
      Tissue: Liver.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT TYR-392.
      Tissue: Mammary gland.
    6. NIEHS SNPs program
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-160; CYS-203; MET-365; TYR-392; ASP-418 AND ASP-419.
    7. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT TYR-392.
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS HIS-160; TYR-392 AND ARG-476.
    10. "Isolation and characterization of a cytochrome P450 of the IIA subfamily from human liver microsomes."
      Maurice M., Emiliani S., Dalet-Beluche I., Derancourt J., Lange R.
      Eur. J. Biochem. 200:511-517(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20, FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
    11. "Purification and characterization of human liver microsomal cytochrome P-450 2A6."
      Yun C.H., Shimada T., Guengerich F.P.
      Mol. Pharmacol. 40:679-685(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-13, FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
    12. "CYP2A6*6, a novel polymorphism in cytochrome p450 2A6, has a single amino acid substitution (R128Q) that inactivates enzymatic activity."
      Kitagawa K., Kunugita N., Kitagawa M., Kawamoto T.
      J. Biol. Chem. 276:17830-17835(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-164, VARIANT CYP2A6*6 GLN-128.
    13. "Isolation and sequence of a human cytochrome P-450 cDNA clone."
      Phillips I.R., Shephard E.A., Ashworth A., Rabin B.R.
      Proc. Natl. Acad. Sci. U.S.A. 82:983-987(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 163-494, VARIANT TYR-392.
    14. "Roles of cytochrome P450 3A enzymes in the 2-hydroxylation of 1,4-cineole, a monoterpene cyclic ether, by rat and human liver microsomes."
      Miyazawa M., Shindo M., Shimada T.
      Xenobiotica 31:713-723(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION AS 1,4-CINEOLE 2-EXO-MONOOXYGENASE, BIOPHYSICOCHEMICAL PROPERTIES.
    15. "Structures of human microsomal cytochrome P450 2A6 complexed with coumarin and methoxsalen."
      Yano J.K., Hsu M.H., Griffin K.J., Stout C.D., Johnson E.F.
      Nat. Struct. Mol. Biol. 12:822-823(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 29-494 IN COMPLEX WITH COUMARIN; HEME AND THE INHIBITOR METHOXSALEN, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    16. "Synthetic inhibitors of cytochrome P-450 2A6: inhibitory activity, difference spectra, mechanism of inhibition, and protein cocrystallization."
      Yano J.K., Denton T.T., Cerny M.A., Zhang X., Johnson E.F., Cashman J.R.
      J. Med. Chem. 49:6987-7001(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 29-494 IN COMPLEX WITH PHENACETIN AND HEME, FUNCTION.
    17. "Key residues controlling phenacetin metabolism by human cytochrome P450 2A enzymes."
      DeVore N.M., Smith B.D., Urban M.J., Scott E.E.
      Drug Metab. Dispos. 36:2582-2590(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 29-494 IN COMPLEX WITH PHENACETIN AND HEME, FUNCTION, CHARACTERIZATION OF VARIANTS LEU-110 AND MET-365, MUTAGENESIS OF ILE-208; SER-213; ILE-300; GLY-301; SER-369 AND ARG-372.
    18. "A single amino acid substitution (Leu160His) in cytochrome P450 CYP2A6 causes switching from 7-hydroxylation to 3-hydroxylation of coumarin."
      Hadidi H., Zahlsen K., Idle J.R., Cholerton S.
      Food Chem. Toxicol. 35:903-907(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF VARIANT CYP2A6*2 HIS-160.
    19. "Identification and characterisation of novel polymorphisms in the CYP2A locus: implications for nicotine metabolism."
      Oscarson M., McLellan R.A., Gullsten H., Agundez J.A., Benitez J., Rautio A., Raunio H., Pelkonen O., Ingelman-Sundberg M.
      FEBS Lett. 460:321-327(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT CYP2A6*5 VAL-479.
    20. "A novel single nucleotide polymorphism altering stability and activity of CYP2a6."
      Ariyoshi N., Sawamura Y., Kamataki T.
      Biochem. Biophys. Res. Commun. 281:810-814(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS CYP2A6*7 THR-471 AND CYP2A6*8 LEU-485.
    21. "Twenty one novel single nucleotide polymorphisms (SNPs) of the CYP2A6 gene in Japanese and Caucasians."
      Kiyotani K., Fujieda M., Yamazaki H., Shimada T., Guengerich F.P., Parkinson A., Nakagawa K., Ishizaki T., Kamataki T.
      Drug Metab. Pharmacokinet. 17:482-487(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS CYP2A6*13 ARG-5; CYP2A6*14 ASN-29; CYP2A6*15 GLU-194 AND CYP2A6*16 SER-203.
    22. "Catalog of 680 variations among eight cytochrome p450 (CYP) genes, nine esterase genes, and two other genes in the Japanese population."
      Saito S., Iida A., Sekine A., Kawauchi S., Higuchi S., Ogawa C., Nakamura Y.
      J. Hum. Genet. 48:249-270(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS ASP-419 AND THR-471.
    23. "Genetic variation in eleven phase I drug metabolism genes in an ethnically diverse population."
      Solus J.F., Arietta B.J., Harris J.R., Sexton D.P., Steward J.Q., McMunn C., Ihrie P., Mehall J.M., Edwards T.L., Dawson E.P.
      Pharmacogenomics 5:895-931(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS ARG-5; ASN-29; LEU-118; GLN-128; PRO-224; MET-365; ASP-418; ASP-419; THR-471; ARG-476 AND LEU-485.
    24. "Novel and established CYP2A6 alleles impair in vivo nicotine metabolism in a population of Black African descent."
      Mwenifumbo J.C., Al Koudsi N., Ho M.K., Zhou Q., Hoffmann E.B., Sellers E.M., Tyndale R.F.
      Hum. Mutat. 29:679-688(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS LEU-110; LEU-118; LEU-128; ALA-131; ASP-418; ASP-419 AND TYR-438.
    25. "A novel CYP2A6 allele, CYP2A6*23, impairs enzyme function in vitro and in vivo and decreases smoking in a population of Black-African descent."
      Ho M.K., Mwenifumbo J.C., Zhao B., Gillam E.M., Tyndale R.F.
      Pharmacogenet. Genomics 18:67-75(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS CYS-203; SER-203 AND MET-365, CHARACTERIZATION OF VARIANT CYS-203.

    Entry informationi

    Entry nameiCP2A6_HUMAN
    AccessioniPrimary (citable) accession number: P11509
    Secondary accession number(s): A7YAE5
    , B2R7F6, P00190, P10890, Q16803, Q4VAT9, Q4VAU0, Q4VAU1, Q9H1Z7, Q9UCU0, Q9UK48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 174 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3