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Protein

Plasma membrane calcium-transporting ATPase 1

Gene

Atp2b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei475 – 47514-aspartylphosphate intermediate
Metal bindingi797 – 7971MagnesiumBy similarity
Metal bindingi801 – 8011MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • PDZ domain binding Source: RGD

GO - Biological processi

  • aging Source: RGD
  • brain development Source: RGD
  • calcium ion export Source: RGD
  • cellular calcium ion homeostasis Source: GO_Central
  • cellular response to corticosterone stimulus Source: RGD
  • cellular response to vitamin D Source: RGD
  • neural retina development Source: RGD
  • response to cold Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-418359. Reduction of cytosolic Ca++ levels.
R-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 1 (EC:3.6.3.8)
Short name:
PMCA1
Alternative name(s):
Plasma membrane calcium ATPase isoform 1
Plasma membrane calcium pump isoform 1
Gene namesi
Name:Atp2b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621303. Atp2b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9796CytoplasmicSequence analysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Topological domaini119 – 15436ExtracellularSequence analysisAdd
BLAST
Transmembranei155 – 17521HelicalSequence analysisAdd
BLAST
Topological domaini176 – 366191CytoplasmicSequence analysisAdd
BLAST
Transmembranei367 – 38620HelicalSequence analysisAdd
BLAST
Topological domaini387 – 41933ExtracellularSequence analysisAdd
BLAST
Transmembranei420 – 43718HelicalSequence analysisAdd
BLAST
Topological domaini438 – 852415CytoplasmicSequence analysisAdd
BLAST
Transmembranei853 – 87220HelicalSequence analysisAdd
BLAST
Topological domaini873 – 88210ExtracellularSequence analysis
Transmembranei883 – 90321HelicalSequence analysisAdd
BLAST
Topological domaini904 – 92320CytoplasmicSequence analysisAdd
BLAST
Transmembranei924 – 94623HelicalSequence analysisAdd
BLAST
Topological domaini947 – 96418ExtracellularSequence analysisAdd
BLAST
Transmembranei965 – 98622HelicalSequence analysisAdd
BLAST
Topological domaini987 – 100519CytoplasmicSequence analysisAdd
BLAST
Transmembranei1006 – 102722HelicalSequence analysisAdd
BLAST
Topological domaini1028 – 103710ExtracellularSequence analysis
Transmembranei1038 – 105922HelicalSequence analysisAdd
BLAST
Topological domaini1060 – 1258199CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical part of cell Source: RGD
  • apical plasma membrane Source: RGD
  • basolateral plasma membrane Source: RGD
  • cytoplasmic side of plasma membrane Source: RGD
  • dendrite membrane Source: RGD
  • dendritic spine membrane Source: RGD
  • extracellular exosome Source: Ensembl
  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
  • membrane raft Source: RGD
  • neuronal cell body membrane Source: RGD
  • nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 12581257Plasma membrane calcium-transporting ATPase 1PRO_0000046212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei17 – 171PhosphoserineCombined sources
Modified residuei338 – 3381PhosphoserineBy similarity
Modified residuei1116 – 11161Phosphothreonine; by PKCBy similarity
Modified residuei1178 – 11781PhosphoserineCombined sources
Modified residuei1193 – 11931PhosphoserineBy similarity
Modified residuei1203 – 12031PhosphothreonineBy similarity
Modified residuei1215 – 12151Phosphoserine; by PKABy similarity
Modified residuei1216 – 12161PhosphoserineBy similarity
Modified residuei1220 – 12201PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP11505.
PRIDEiP11505.

PTM databases

iPTMnetiP11505.
PhosphoSiteiP11505.
SwissPalmiP11505.

Expressioni

Tissue specificityi

Isoform B is ubiquitously expressed. Isoforms A and E have only been found in brain cortex. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.

Gene expression databases

ExpressionAtlasiP11505. baseline and differential.
GenevisibleiP11505. RN.

Interactioni

Subunit structurei

Interacts with PDZD11. Interacts with SLC35G1 and STIM1.By similarity

GO - Molecular functioni

  • PDZ domain binding Source: RGD

Protein-protein interaction databases

BioGridi248230. 3 interactions.
STRINGi10116.ENSRNOP00000005491.

Structurei

3D structure databases

ProteinModelPortaliP11505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1100 – 111718Calmodulin-binding subdomain ABy similarityAdd
BLAST
Regioni1118 – 112710Calmodulin-binding subdomain BBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi296 – 2994Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiP11505.
KOiK05850.
OrthoDBiEOG7SN8BN.
PhylomeDBiP11505.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 3 hits.
PfamiPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D (identifier: P11505-1) [UniParc]FASTAAdd to basket

Also known as: CIV

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMANNSVA YSGVKNSLKE ANHDGDFGIT LAELRALMEL RSTDALRKIQ
60 70 80 90 100
ESYGDVYGIC TKLKTSPNEG LSGNPADLER REAVFGKNFI PPKKPKTFLQ
110 120 130 140 150
LVWEALQDVT LIILEIAAIV SLGLSFYQPP EGDNALCGEV SVGEEEGEGE
160 170 180 190 200
TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ FRGLQSRIEQ EQKFTVIRGG
210 220 230 240 250
QVIQIPVADI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE SSLTGESDHV
260 270 280 290 300
KKSLDKDPLL LSGTHVMEGS GRMVVTAVGV NSQTGIIFTL LGAGGEEEEK
310 320 330 340 350
KDEKKKEKKN KKQDGAIENR NKAKAQDGAA MEMQPLKSEE GGDGDEKDKK
360 370 380 390 400
KANLPKKEKS VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFVIDTFWVQ
410 420 430 440 450
KRPWLAECTP IYIQYFVKFF IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM
460 470 480 490 500
MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA YINEKHYKKV
510 520 530 540 550
PEPEAIPPNI LSYLVTGISV NCAYTSKILP PEKEGGLPRH VGNKTECALL
560 570 580 590 600
GFLLDLKRDY QDVRNEIPEE ALYKVYTFNS VRKSMSTVLK NSDGSFRIFS
610 620 630 640 650
KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL
660 670 680 690 700
AFRDFPAGEP EPEWDNENDV VTGLTCIAVV GIEDPVRPEV PEAIKKCQRA
710 720 730 740 750
GITVRMVTGD NINTARAIAT KCGILHPGED FLCLEGKDFN RRIRNEKGEI
760 770 780 790 800
EQERIDKIWP KLRVLARSSP TDKHTLVKGI IDSTVSEQRQ VVAVTGDGTN
810 820 830 840 850
DGPALKKADV GFAMGIAGTD VAKEASDIIL TDDNFTSIVK AVMWGRNVYD
860 870 880 890 900
SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL
910 920 930 940 950
ALATEPPTES LLLRKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLFAG
960 970 980 990 1000
EKFFDIDSGR NAPLHAPPSE HYTIVFNTFV LMQLFNEINA RKIHGERNVF
1010 1020 1030 1040 1050
EGIFNNAIFC TIVLGTFVVQ IIIVQFGGKP FSCSELSIEQ WLWSIFLGMG
1060 1070 1080 1090 1100
TLLWGQLIST IPTSRLKFLK EAGHGTQKEE IPEEELAEDV EEIDHAEREL
1110 1120 1130 1140 1150
RRGQILWFRG LNRIQTQMDV VNAFQSGGSI QGALRRQPSI ASQHHDVTNV
1160 1170 1180 1190 1200
STPTHIRVVN AFRSSLYEGL EKPESRSSIH NFMTHPEFRI EDSEPLIPLI
1210 1220 1230 1240 1250
DDTDAEDDAP TKRNSSPPPS PNKNNNAVDS GIHLTIEMNK SATSSSPGSP

LHSLETSL
Length:1,258
Mass (Da):138,719
Last modified:June 1, 2001 - v2
Checksum:iD1F19DC331F76B61
GO
Isoform A (identifier: P11505-2) [UniParc]FASTAAdd to basket

Also known as: CII

The sequence of this isoform differs from the canonical sequence as follows:
     1156-1258: IRVVNAFRSS...SPLHSLETSL → VVFSSSTASTPVGYPSGECIS

Show »
Length:1,176
Mass (Da):129,510
Checksum:i96F8E55209C51F2B
GO
Isoform B (identifier: P11505-3) [UniParc]FASTAAdd to basket

Also known as: CI

The sequence of this isoform differs from the canonical sequence as follows:
     1118-1155: Missing.

Show »
Length:1,220
Mass (Da):134,693
Checksum:i29BD18FC2B024746
GO
Isoform C (identifier: P11505-4) [UniParc]FASTAAdd to basket

Also known as: CIII

The sequence of this isoform differs from the canonical sequence as follows:
     1147-1155: Missing.

Show »
Length:1,249
Mass (Da):137,782
Checksum:i9DFE64B0DE56EAB5
GO
Isoform E (identifier: P11505-5) [UniParc]FASTAAdd to basket

Also known as: CV

The sequence of this isoform differs from the canonical sequence as follows:
     1156-1258: IRVVNAFRSS...SPLHSLETSL → VVFSSSTASTPVGSEW

Show »
Length:1,171
Mass (Da):129,076
Checksum:iB3C355489CBA0FE2
GO
Isoform K (identifier: P11505-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1021-1056: Missing.
     1118-1155: Missing.

Show »
Length:1,184
Mass (Da):130,625
Checksum:iC93FBF49CB01B7D2
GO

Sequence cautioni

The sequence AAA50878.1 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1125 – 11251Q → R in AAD46085 (PubMed:10858669).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1021 – 105636Missing in isoform K. 1 PublicationVSP_000379Add
BLAST
Alternative sequencei1118 – 115538Missing in isoform B and isoform K. 1 PublicationVSP_000380Add
BLAST
Alternative sequencei1147 – 11559Missing in isoform C. CuratedVSP_000381
Alternative sequencei1156 – 1258103IRVVN…LETSL → VVFSSSTASTPVGYPSGECI S in isoform A. 1 PublicationVSP_000382Add
BLAST
Alternative sequencei1156 – 1258103IRVVN…LETSL → VVFSSSTASTPVGSEW in isoform E. CuratedVSP_000383Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03753 mRNA. Translation: AAA73898.1.
L04739 Genomic DNA. Translation: AAA50878.1. Sequence problems.
AF076783 mRNA. Translation: AAD46085.1.
PIRiA28065.
D45213.
RefSeqiNP_445763.1. NM_053311.1. [P11505-2]
UniGeneiRn.228999.
Rn.7208.

Genome annotation databases

EnsembliENSRNOT00000041354; ENSRNOP00000040092; ENSRNOG00000004026. [P11505-2]
ENSRNOT00000066174; ENSRNOP00000059397; ENSRNOG00000004026. [P11505-2]
GeneIDi29598.
KEGGirno:29598.
UCSCiRGD:621303. rat. [P11505-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03753 mRNA. Translation: AAA73898.1.
L04739 Genomic DNA. Translation: AAA50878.1. Sequence problems.
AF076783 mRNA. Translation: AAD46085.1.
PIRiA28065.
D45213.
RefSeqiNP_445763.1. NM_053311.1. [P11505-2]
UniGeneiRn.228999.
Rn.7208.

3D structure databases

ProteinModelPortaliP11505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248230. 3 interactions.
STRINGi10116.ENSRNOP00000005491.

PTM databases

iPTMnetiP11505.
PhosphoSiteiP11505.
SwissPalmiP11505.

Proteomic databases

PaxDbiP11505.
PRIDEiP11505.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041354; ENSRNOP00000040092; ENSRNOG00000004026. [P11505-2]
ENSRNOT00000066174; ENSRNOP00000059397; ENSRNOG00000004026. [P11505-2]
GeneIDi29598.
KEGGirno:29598.
UCSCiRGD:621303. rat. [P11505-1]

Organism-specific databases

CTDi490.
RGDi621303. Atp2b1.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiP11505.
KOiK05850.
OrthoDBiEOG7SN8BN.
PhylomeDBiP11505.

Enzyme and pathway databases

ReactomeiR-RNO-418359. Reduction of cytosolic Ca++ levels.
R-RNO-5578775. Ion homeostasis.
R-RNO-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP11505.

Gene expression databases

ExpressionAtlasiP11505. baseline and differential.
GenevisibleiP11505. RN.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 3 hits.
PfamiPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two isoforms of the plasma membrane Ca2+-transporting ATPase from rat brain. Structural and functional domains exhibit similarity to Na+,K+- and other cation transport ATPases."
    Shull G.E., Greeb J.
    J. Biol. Chem. 263:8646-8657(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
  2. "Expression of multiple plasma membrane Ca(2+)-ATPases in rat pancreatic islet cells."
    Kamagate A., Herchuelz A., Bollen A., Van Eylen F.
    Cell Calcium 27:231-246(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 926-1164 (ISOFORM K).
    Tissue: Pancreatic islet.
  3. "Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
    Keeton T.P., Burk S.E., Shull G.E.
    J. Biol. Chem. 268:2740-2748(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1057-1232, ALTERNATIVE SPLICING (ISOFORMS A; B; C; D AND E).
    Strain: Sprague-Dawley.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17 AND SER-1178, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAT2B1_RAT
AccessioniPrimary (citable) accession number: P11505
Secondary accession number(s): Q63442, Q9R1L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.