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P11505 (AT2B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasma membrane calcium-transporting ATPase 1
Short name=PMCA1
EC=3.6.3.8
Alternative name(s):
Plasma membrane calcium ATPase isoform 1
Plasma membrane calcium pump isoform 1
Gene names
Name:Atp2b1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1258 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activity

ATP + H2O + Ca2+[side 1] = ADP + phosphate + Ca2+[side 2].

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Isoform B is ubiquitously expressed. Isoforms A and E have only been found in brain cortex. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Sequence caution

The sequence AAA50878.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Calmodulin-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processATP biosynthetic process

Inferred from electronic annotation. Source: InterPro

aging

Inferred from expression pattern. Source: RGD

brain development

Inferred from expression pattern. Source: RGD

cellular response to corticosterone stimulus

Inferred from expression pattern. Source: RGD

cellular response to vitamin D

Inferred from expression pattern. Source: RGD

negative regulation of smooth muscle cell proliferation

Inferred from mutant phenotype. Source: RGD

neural retina development

Inferred from expression pattern. Source: RGD

response to cold

Inferred from expression pattern. Source: RGD

   Cellular componentapical plasma membrane

Inferred from direct assay. Source: RGD

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane raft

Inferred from direct assay. Source: RGD

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

PDZ domain binding

Inferred from physical interaction. Source: RGD

calcium-transporting ATPase activity

Inferred from electronic annotation. Source: EC

calmodulin binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform D (identifier: P11505-1)

Also known as: CIV;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: P11505-2)

Also known as: CII;

The sequence of this isoform differs from the canonical sequence as follows:
     1156-1258: IRVVNAFRSS...SPLHSLETSL → VVFSSSTASTPVGYPSGECIS
Isoform B (identifier: P11505-3)

Also known as: CI;

The sequence of this isoform differs from the canonical sequence as follows:
     1118-1155: Missing.
Isoform C (identifier: P11505-4)

Also known as: CIII;

The sequence of this isoform differs from the canonical sequence as follows:
     1147-1155: Missing.
Isoform E (identifier: P11505-5)

Also known as: CV;

The sequence of this isoform differs from the canonical sequence as follows:
     1156-1258: IRVVNAFRSS...SPLHSLETSL → VVFSSSTASTPVGSEW
Isoform K (identifier: P11505-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1021-1056: Missing.
     1118-1155: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12581258Plasma membrane calcium-transporting ATPase 1
PRO_0000046212

Regions

Topological domain1 – 9797Cytoplasmic Potential
Transmembrane98 – 11821Helical; Potential
Topological domain119 – 15436Extracellular Potential
Transmembrane155 – 17521Helical; Potential
Topological domain176 – 366191Cytoplasmic Potential
Transmembrane367 – 38620Helical; Potential
Topological domain387 – 41933Extracellular Potential
Transmembrane420 – 43718Helical; Potential
Topological domain438 – 852415Cytoplasmic Potential
Transmembrane853 – 87220Helical; Potential
Topological domain873 – 88210Extracellular Potential
Transmembrane883 – 90321Helical; Potential
Topological domain904 – 92320Cytoplasmic Potential
Transmembrane924 – 94623Helical; Potential
Topological domain947 – 96418Extracellular Potential
Transmembrane965 – 98622Helical; Potential
Topological domain987 – 100519Cytoplasmic Potential
Transmembrane1006 – 102722Helical; Potential
Topological domain1028 – 103710Extracellular Potential
Transmembrane1038 – 105922Helical; Potential
Topological domain1060 – 1258199Cytoplasmic Potential
Region1100 – 111718Calmodulin-binding subdomain A By similarity
Region1118 – 112710Calmodulin-binding subdomain B By similarity
Compositional bias296 – 2994Poly-Glu

Sites

Active site47514-aspartylphosphate intermediate
Metal binding7971Magnesium By similarity
Metal binding8011Magnesium By similarity

Amino acid modifications

Modified residue171Phosphoserine By similarity
Modified residue11161Phosphothreonine; by PKC By similarity
Modified residue11931Phosphoserine By similarity
Modified residue12031Phosphothreonine By similarity
Modified residue12151Phosphoserine; by PKA By similarity
Modified residue12201Phosphoserine By similarity
Modified residue12461Phosphoserine By similarity
Modified residue12491Phosphoserine By similarity
Modified residue12531Phosphoserine By similarity
Modified residue12561Phosphothreonine By similarity
Modified residue12571Phosphoserine By similarity

Natural variations

Alternative sequence1021 – 105636Missing in isoform K.
VSP_000379
Alternative sequence1118 – 115538Missing in isoform B and isoform K.
VSP_000380
Alternative sequence1147 – 11559Missing in isoform C.
VSP_000381
Alternative sequence1156 – 1258103IRVVN…LETSL → VVFSSSTASTPVGYPSGECI S in isoform A.
VSP_000382
Alternative sequence1156 – 1258103IRVVN…LETSL → VVFSSSTASTPVGSEW in isoform E.
VSP_000383

Experimental info

Sequence conflict11251Q → R in AAD46085. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform D (CIV) [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: D1F19DC331F76B61

FASTA1,258138,719
        10         20         30         40         50         60 
MGDMANNSVA YSGVKNSLKE ANHDGDFGIT LAELRALMEL RSTDALRKIQ ESYGDVYGIC 

        70         80         90        100        110        120 
TKLKTSPNEG LSGNPADLER REAVFGKNFI PPKKPKTFLQ LVWEALQDVT LIILEIAAIV 

       130        140        150        160        170        180 
SLGLSFYQPP EGDNALCGEV SVGEEEGEGE TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ 

       190        200        210        220        230        240 
FRGLQSRIEQ EQKFTVIRGG QVIQIPVADI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE 

       250        260        270        280        290        300 
SSLTGESDHV KKSLDKDPLL LSGTHVMEGS GRMVVTAVGV NSQTGIIFTL LGAGGEEEEK 

       310        320        330        340        350        360 
KDEKKKEKKN KKQDGAIENR NKAKAQDGAA MEMQPLKSEE GGDGDEKDKK KANLPKKEKS 

       370        380        390        400        410        420 
VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFVIDTFWVQ KRPWLAECTP IYIQYFVKFF 

       430        440        450        460        470        480 
IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL 

       490        500        510        520        530        540 
TMNRMTVVQA YINEKHYKKV PEPEAIPPNI LSYLVTGISV NCAYTSKILP PEKEGGLPRH 

       550        560        570        580        590        600 
VGNKTECALL GFLLDLKRDY QDVRNEIPEE ALYKVYTFNS VRKSMSTVLK NSDGSFRIFS 

       610        620        630        640        650        660 
KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL AFRDFPAGEP 

       670        680        690        700        710        720 
EPEWDNENDV VTGLTCIAVV GIEDPVRPEV PEAIKKCQRA GITVRMVTGD NINTARAIAT 

       730        740        750        760        770        780 
KCGILHPGED FLCLEGKDFN RRIRNEKGEI EQERIDKIWP KLRVLARSSP TDKHTLVKGI 

       790        800        810        820        830        840 
IDSTVSEQRQ VVAVTGDGTN DGPALKKADV GFAMGIAGTD VAKEASDIIL TDDNFTSIVK 

       850        860        870        880        890        900 
AVMWGRNVYD SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL 

       910        920        930        940        950        960 
ALATEPPTES LLLRKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLFAG EKFFDIDSGR 

       970        980        990       1000       1010       1020 
NAPLHAPPSE HYTIVFNTFV LMQLFNEINA RKIHGERNVF EGIFNNAIFC TIVLGTFVVQ 

      1030       1040       1050       1060       1070       1080 
IIIVQFGGKP FSCSELSIEQ WLWSIFLGMG TLLWGQLIST IPTSRLKFLK EAGHGTQKEE 

      1090       1100       1110       1120       1130       1140 
IPEEELAEDV EEIDHAEREL RRGQILWFRG LNRIQTQMDV VNAFQSGGSI QGALRRQPSI 

      1150       1160       1170       1180       1190       1200 
ASQHHDVTNV STPTHIRVVN AFRSSLYEGL EKPESRSSIH NFMTHPEFRI EDSEPLIPLI 

      1210       1220       1230       1240       1250 
DDTDAEDDAP TKRNSSPPPS PNKNNNAVDS GIHLTIEMNK SATSSSPGSP LHSLETSL

« Hide

Isoform A (CII) [UniParc].

Checksum: 96F8E55209C51F2B
Show »

FASTA1,176129,510
Isoform B (CI) [UniParc].

Checksum: 29BD18FC2B024746
Show »

FASTA1,220134,693
Isoform C (CIII) [UniParc].

Checksum: 9DFE64B0DE56EAB5
Show »

FASTA1,249137,782
Isoform E (CV) [UniParc].

Checksum: B3C355489CBA0FE2
Show »

FASTA1,171129,076
Isoform K [UniParc].

Checksum: C93FBF49CB01B7D2
Show »

FASTA1,184130,625

References

[1]"Molecular cloning of two isoforms of the plasma membrane Ca2+-transporting ATPase from rat brain. Structural and functional domains exhibit similarity to Na+,K+- and other cation transport ATPases."
Shull G.E., Greeb J.
J. Biol. Chem. 263:8646-8657(1988) [PubMed: 2837461] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]"Expression of multiple plasma membrane Ca(2+)-ATPases in rat pancreatic islet cells."
Kamagate A., Herchuelz A., Bollen A., Van Eylen F.
Cell Calcium 27:231-246(2000) [PubMed: 10858669] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 926-1164 (ISOFORM K).
Tissue: Pancreatic islet.
[3]"Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4."
Keeton T.P., Burk S.E., Shull G.E.
J. Biol. Chem. 268:2740-2748(1993) [PubMed: 8428948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1057-1232, ALTERNATIVE SPLICING (ISOFORMS A; B; C; D AND E).
Strain: Sprague-Dawley.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J03753 mRNA. Translation: AAA73898.1.
L04739 Genomic DNA. Translation: AAA50878.1. Sequence problems.
AF076783 mRNA. Translation: AAD46085.1.
IPIIPI00194873.
IPI00231267.
IPI00231268.
IPI00231269.
IPI00231270.
IPI00231271.
PIRA28065.
D45213.
RefSeqNP_445763.1. NM_053311.1.
UniGeneRn.7208.

3D structure databases

ProteinModelPortalP11505.
ModBaseSearch...

Protein-protein interaction databases

STRINGP11505.

PTM databases

PhosphoSiteP11505.

Proteomic databases

PRIDEP11505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000041354; ENSRNOP00000040092; ENSRNOG00000004026.
GeneID29598.
KEGGrno:29598.
UCSCNM_053311. rat.

Organism-specific databases

CTD490.
RGD621303. Atp2b1.

Phylogenomic databases

eggNOGroNOG14596.
GeneTreeENSGT00510000046331.
HOVERGENHBG061286.
InParanoidP11505.
OrthoDBEOG4KPT93.
PhylomeDBP11505.

Gene expression databases

ArrayExpressP11505.
GenevestigatorP11505.
GermOnlineENSRNOG00000004026. Rattus norvegicus.

Family and domain databases

InterProIPR022141. ATP_Ca_trans_C.
IPR023306. ATPase_cation_domN.
IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR006408. ATPase_P-typ_Ca-transp_PMCA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 1 hit.
KOK05850.
PfamPF12424. ATP_Ca_trans_C. 2 hits.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF81660. ATPase_cation_domN. 1 hit.
SSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609750.

Entry information

Entry nameAT2B1_RAT
AccessionPrimary (citable) accession number: P11505
Secondary accession number(s): Q63442, Q9R1L7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 1, 2001
Last modified: January 25, 2012
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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