P11504 (CAT_CLODI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloramphenicol acetyltransferase
Short name=CAT
EC=2.3.1.28

Gene names

Name:

catD

Organism

Clostridium difficile

Taxonomic identifier

1496 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Peptostreptococcaceae

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is an effector of chloramphenicol resistance in bacteria.
Catalytic activity	Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.
Subunit structure	Homotrimer.
Sequence similarities	Belongs to the chloramphenicol acetyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chloramphenicol O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Chloramphenicol acetyltransferase		PRO_0000165860
Sites
	Active site	186	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11504 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 790F8448030E8B55	FASTA	212	24,836
	10 20 30 40 50 60 MVFEKIDKNS WNRKEYFDHY FASVPCTYSM TVKVDITQIK EKGMKLYPAM LYYIAMIVNR 70 80 90 100 110 120 HSEFRTAINQ DGELGIYDEM IPSYTIFHND TETFSSLWTE CKSDFKSFLA DYESDTQRYG 130 140 150 160 170 180 NNHRMEGKPN APENIFNVSM IPWSTFDGFN LNLQKGYDYL IPIFTMGKII KKDNKIILPL 190 200 210 AIQVHHAVCD GFHICRFVNE LQELIIVTQV CL

References

[1]	"Nucleotide sequence of a chloramphenicol acetyl transferase gene from Clostridium difficile." Wren B.W., Mullany P., Clayton C., Tabaqchali S. Nucleic Acids Res. 17:4877-4877(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Type W / Isolate W1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15100 Genomic DNA. Translation: CAA33203.1.
PIR	S04711.
3D structure databases
ProteinModelPortal	P11504.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.559.10. 1 hit.
InterPro	IPR023213. CAT-like_dom. IPR018372. Chloramphenicol_AcTrfase_AS. IPR001707. Cmp_AcTrfase. [Graphical view]
Pfam	PF00302. CAT. 1 hit. [Graphical view]
PIRSF	PIRSF000440. CAT. 1 hit.
ProDom	PD002660. Cmp_AcTrfase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM01059. CAT. 1 hit. [Graphical view]
PROSITE	PS00100. CAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAT_CLODI

Accession

Primary (citable) accession number: P11504

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program