P11502 (PTLA_LACCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactose-specific phosphotransferase enzyme IIA component
EC=2.7.1.-

Alternative name(s):
EIIA-Lac
EIII-Lac
PTS system lactose-specific EIIA component

Gene names

Name:

lacF

Organism

Lactobacillus casei

Taxonomic identifier

1582 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus casei group

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Cofactor	Binds 1 magnesium ion per trimer By similarity.
Enzyme regulation	Induced by lactose and inhibited by glucose.
Subunit structure	Homotrimer.
Subcellular location	Cytoplasm By similarity.
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-3 domain.
Sequence caution	The sequence CAB02558.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 112	112	Lactose-specific phosphotransferase enzyme IIA component		PRO_0000186596
Regions
	Domain	6 – 104	99	PTS EIIA type-3
Sites
	Active site	80	1	Tele-phosphohistidine intermediate; by HPr By similarity
	Metal binding	83	1	Magnesium; shared with all trimeric partners By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11502 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 648B7E9D70D79E07	FASTA	112	12,496
	10 20 30 40 50 60 MMATKEEISM VGFALVAYAG DARTAAVHAL DAAEAGDFDK ANELVEKAQQ DINEAHNQQT 70 80 90 100 110 QLLSQEAGGA EMDVTFIMVH GQDTLMTTML LIDETRYMIR MFKRIKELEN KQ

References

[1]	"Molecular cloning and nucleotide sequence of the factor IIIlac gene of Lactobacillus casei." Alpert C.-A., Chassy B.M. Gene 62:277-288(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 64H.
[2]	"Establishing a model to study the regulation of the lactose operon in Lactobacillus casei." Gosalbes M.J., Monedero V., Alpert C.-A., Perez-Martinez G. FEMS Microbiol. Lett. 148:83-89(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 393 / DSM 20011 / JCM 1134 / NBRC 15883 / NCIMB 11970 / NCDO 161.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20150 Genomic DNA. Translation: AAA25239.1. Z80834 Genomic DNA. Translation: CAB02558.1. Different initiation.
PIR	B29898.
3D structure databases
ProteinModelPortal	P11502.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.58.80. 1 hit.
InterPro	IPR003188. PTS_IIA_lac. [Graphical view]
Pfam	PF02255. PTS_IIA. 1 hit. [Graphical view]
PIRSF	PIRSF000699. PTS_IILac_III. 1 hit.
SUPFAM	SSF46973. Ptrans_IIA. 1 hit.
TIGRFAMs	TIGR00823. EIIA-LAC. 1 hit.
PROSITE	PS51095. PTS_EIIA_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTLA_LACCA

Accession

Primary (citable) accession number: P11502

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program