P11494 (BDS1_ANESU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Antihypertensive protein BDS-1 Alternative name(s): Blood depressing substance I Short name=BDS-I
Organism	Anemonia sulcata (Mediterranean snakelocks sea anemone) (Anemonia viridis)
Taxonomic identifier	6108 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Actiniaria › Nynantheae › Actiniidae › Anemonia

Protein attributes

Sequence length	43 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Blocks specifically the Kv3.4/KCNC4 potassium channel. Reduces blood pressure. Ref.2
Subcellular location	Secreted. Nematocyst.
Sequence similarities	Belongs to the sea anemone type 3 (BDS) potassium channel toxin family.

Ontologies

Keywords
Cellular component	Nematocyst Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nematocyst Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 43

43

Antihypertensive protein BDS-1

PRO_0000221541

Amino acid modifications

Disulfide bond

4 ↔ 39

Ref.3 Ref.4

Disulfide bond

6 ↔ 32

Ref.3 Ref.4

Disulfide bond

22 ↔ 40

Ref.3 Ref.4

Natural variations

Natural variant

18

1

L → F.

Secondary structure

1

.

.

.

.

.

.

.

.

.

43

Helix Strand Turn

Details...

Beta strand

4 – 6

3

Beta strand

14 – 16

3

Beta strand

31 – 34

4

Beta strand

37 – 40

4

Sequences

	Sequence		Length	Mass (Da)
	P11494 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 7C17846E88E2F1D8	FASTA	43	4,714
	10 20 30 40 AAPCFCSGKP GRGDLWILRG TCPGGYGYTS NCYKWPNICC YPH

References

[1]	"Polypeptides, process for their preparation, and their use as hypotensive active compounds." Doppelfeld I.-S., Henschen-Edman A., Graf E., Zwick J., Beress L., Etschenberg E. Patent number DE3324689, 17-JAN-1985 Cited for: PROTEIN SEQUENCE.
[2]	"Sea anemone peptides with a specific blocking activity against the fast inactivating potassium channel Kv3.4." Diochot S., Schweitz H., Beress L., Lazdunski M. J. Biol. Chem. 273:6744-6749(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION.
[3]	"A proton nuclear magnetic resonance study of the antihypertensive and antiviral protein BDS-I from the sea anemone Anemonia sulcata: sequential and stereospecific resonance assignment and secondary structure." Driscoll P.C., Clore G.M., Beress L., Gronenborn A.M. Biochemistry 28:2178-2187(1989) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.
[4]	"Determination of the three-dimensional solution structure of the antihypertensive and antiviral protein BDS-I from the sea anemone Anemonia sulcata: a study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing." Driscoll P.C., Gronenborn A.M., Beress L., Clore G.M. Biochemistry 28:2188-2198(1989) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

Sequence databases

PIR

A33041.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BDS	NMR	-	A	1-43	[»]
2BDS	NMR	-	A	1-43	[»]

ProteinModelPortal

P11494.

SMR

P11494. Positions 1-43.

ModBase

Search...

Protein family/group databases

TCDB

8.B.11.1.3. sea anemone peptide toxin (APETx) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.20.20.10. 1 hit.

InterPro

IPR012414. BDS_K_chnl_tox.
IPR023355. Myo_neuro_toxin.
[Graphical view]

Pfam

PF07936. Defensin_4. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P11494.

Entry information

Entry name

BDS1_ANESU

Accession

Primary (citable) accession number: P11494

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program