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Protein

Repressible alkaline phosphatase

Gene

PHO8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate. Acts as a fructose-2,6-bisphosphate 6-phosphatase (PubMed:1848184).1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation1 Publication
(2E,6E)-farnesyl diphosphate + H2O = (2E,6E)-farnesol + diphosphate.1 Publication
Beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructofuranose 2-phosphate + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

pH dependencei

Optimum pH is 7.0 for farnesyl diphosphatase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi75MagnesiumBy similarity1
Metal bindingi75Zinc 2By similarity1
Active sitei123Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi176MagnesiumBy similarity1
Metal bindingi325MagnesiumBy similarity1
Metal bindingi330Zinc 1By similarity1
Metal bindingi334Zinc 1By similarity1
Metal bindingi373Zinc 2By similarity1
Metal bindingi374Zinc 2By similarity1
Metal bindingi484Zinc 1By similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: SGD
  • fructose-2,6-bisphosphate 6-phosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • nicotinamide nucleotide metabolic process Source: SGD
  • protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YDR481C-MONOMER.
YEAST:YDR481C-MONOMER.
ReactomeiR-SCE-1483166. Synthesis of PA.
R-SCE-8935690. Miscellaneous digestion events.

Names & Taxonomyi

Protein namesi
Recommended name:
Repressible alkaline phosphatase (EC:3.1.3.1)
Alternative name(s):
Fructose-2,6-bisphosphate 6-phosphatase (EC:3.1.3.54)
Membrane-bound repressible alkaline phosphatase
Cleaved into the following chain:
Alternative name(s):
Farnesyl diphosphatase
Gene namesi
Name:PHO8
Ordered Locus Names:YDR481C
ORF Names:D8035.24
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR481C.
SGDiS000002889. PHO8.

Subcellular locationi

Repressible alkaline phosphatase :
Soluble alkaline phosphatase :
  • Cytoplasm

  • Note: The truncated version of the protein is soluble.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33Cytoplasmic1 PublicationAdd BLAST33
Transmembranei34 – 59HelicalSequence analysisAdd BLAST26
Topological domaini60 – ?Vacuolar1 Publication

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000024018? – 566Removed in mature form
ChainiPRO_00000240171 – ?Repressible alkaline phosphatase
ChainiPRO_000040119863 – ?Soluble alkaline phosphatase

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei123PhosphoserineCombined sources1
Glycosylationi268N-linked (GlcNAc...)1
Glycosylationi401N-linked (GlcNAc...)1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP11491.
PRIDEiP11491.

PTM databases

iPTMnetiP11491.

Interactioni

Protein-protein interaction databases

BioGridi32533. 20 interactors.
IntActiP11491. 2 interactors.
MINTiMINT-4482897.

Structurei

3D structure databases

ProteinModelPortaliP11491.
SMRiP11491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099116.
InParanoidiP11491.
KOiK01077.
OMAiLKSYNGA.
OrthoDBiEOG092C1UXR.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 2 hits.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTHTLPSEQ TRLVPGSDSS SRPKKRRISK RSKIIVSTVV CIGLLLVLVQ
60 70 80 90 100
LAFPSSFALR SASHKKKNVI FFVTDGMGPA SLSMARSFNQ HVNDLPIDDI
110 120 130 140 150
LTLDEHFIGS SRTRSSDSLV TDSAAGATAF ACALKSYNGA IGVDPHHRPC
160 170 180 190 200
GTVLEAAKLA GYLTGLVVTT RITDATPASF SSHVDYRWQE DLIATHQLGE
210 220 230 240 250
YPLGRVVDLL MGGGRSHFYP QGEKASPYGH HGARKDGRDL IDEAQSNGWQ
260 270 280 290 300
YVGDRKNFDS LLKSHGENVT LPFLGLFADN DIPFEIDRDE KEYPSLKEQV
310 320 330 340 350
KVALGALEKA SNEDKDSNGF FLMVEGSRID HAGHQNDPAS QVREVLAFDE
360 370 380 390 400
AFQYVLEFAE NSDTETVLVS TSDHETGGLV TSRQVTASYP QYVWYPQVLA
410 420 430 440 450
NATHSGEFLK RKLVDFVHEH KGASSKIENF IKHEILEKDL GIYDYTDSDL
460 470 480 490 500
ETLIHLDDNA NAIQDKLNDM VSFRAQIGWT THGHSAVDVN IYAYANKKAT
510 520 530 540 550
WSYVLNNLQG NHENTEVGQF LENFLELNLN EVTDLIRDTK HTSDFDATEI
560
ASEVQHYDEY YHELTN
Length:566
Mass (Da):63,004
Last modified:July 15, 1998 - v2
Checksum:i9FA2E87B068FF0DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5T → R in AAA34871 (PubMed:3319783).Curated1
Sequence conflicti55S → T in AAA34871 (PubMed:3319783).Curated1
Sequence conflicti59L → I in AAA34871 (PubMed:3319783).Curated1
Sequence conflicti132C → S in AAA34871 (PubMed:3319783).Curated1
Sequence conflicti271L → F in AAA34871 (PubMed:3319783).Curated1
Sequence conflicti328R → G in AAU09711 (PubMed:17322287).Curated1
Sequence conflicti447D → E in AAA34871 (PubMed:3319783).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21134 Genomic DNA. Translation: AAA34871.1.
U33050 Genomic DNA. Translation: AAB64930.1.
AY723794 Genomic DNA. Translation: AAU09711.1.
BK006938 Genomic DNA. Translation: DAA12314.1.
PIRiS69648.
RefSeqiNP_010769.3. NM_001180789.3.

Genome annotation databases

EnsemblFungiiYDR481C; YDR481C; YDR481C.
GeneIDi852092.
KEGGisce:YDR481C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21134 Genomic DNA. Translation: AAA34871.1.
U33050 Genomic DNA. Translation: AAB64930.1.
AY723794 Genomic DNA. Translation: AAU09711.1.
BK006938 Genomic DNA. Translation: DAA12314.1.
PIRiS69648.
RefSeqiNP_010769.3. NM_001180789.3.

3D structure databases

ProteinModelPortaliP11491.
SMRiP11491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32533. 20 interactors.
IntActiP11491. 2 interactors.
MINTiMINT-4482897.

PTM databases

iPTMnetiP11491.

Proteomic databases

MaxQBiP11491.
PRIDEiP11491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR481C; YDR481C; YDR481C.
GeneIDi852092.
KEGGisce:YDR481C.

Organism-specific databases

EuPathDBiFungiDB:YDR481C.
SGDiS000002889. PHO8.

Phylogenomic databases

GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099116.
InParanoidiP11491.
KOiK01077.
OMAiLKSYNGA.
OrthoDBiEOG092C1UXR.

Enzyme and pathway databases

BioCyciMetaCyc:YDR481C-MONOMER.
YEAST:YDR481C-MONOMER.
ReactomeiR-SCE-1483166. Synthesis of PA.
R-SCE-8935690. Miscellaneous digestion events.

Miscellaneous databases

PROiP11491.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 2 hits.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB_YEAST
AccessioniPrimary (citable) accession number: P11491
Secondary accession number(s): D6VTA4, E9P949, Q03374
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.