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Protein

Alpha-defensin 1

Gene

Defa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

GO - Biological processi

  • cellular response to lipopolysaccharide Source: MGI
  • defense response to bacterium Source: MGI
  • defense response to Gram-negative bacterium Source: MGI
  • defense response to Gram-positive bacterium Source: MGI
  • response to drug Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-defensin 1
Alternative name(s):
Cryptdin-1
Defensin-related cryptdin peptide
Gene namesi
Name:Defa1
Synonyms:Defcr, Defcr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:94880. Defa1.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Propeptidei20 – 5839Sequence analysisPRO_0000006817Add
BLAST
Peptidei59 – 9335Alpha-defensin 1PRO_0000006818Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 92By similarity
Disulfide bondi66 ↔ 81By similarity
Disulfide bondi71 ↔ 91By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PeptideAtlasiP11477.
PRIDEiP11477.

Expressioni

Tissue specificityi

Paneth cells of the small bowel.

Developmental stagei

Accumulates to high levels in mouse intestinal crypt epithelium during postnatal development.

Gene expression databases

CleanExiMM_DEFA1.

Structurei

3D structure databases

ProteinModelPortaliP11477.
SMRiP11477. Positions 62-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000233351.
HOVERGENiHBG011703.
InParanoidiP11477.
KOiK05230.
PhylomeDBiP11477.

Family and domain databases

InterProiIPR006081. Alpha-defensin.
IPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLVLLFAL VLLGFQVQAD SIQNTDEETK TEEQPGEEDQ AVSVSFGDPE
60 70 80 90
GTSLQEESLR DLVCYCRSRG CKGRERMNGT CRKGHLLYTL CCR
Length:93
Mass (Da):10,461
Last modified:August 1, 1990 - v2
Checksum:i3CD0B6FB9644A22D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31K → T in AAB60675 (PubMed:8188287).Curated
Sequence conflicti8 – 81F → S in AAB60675 (PubMed:8188287).Curated
Sequence conflicti14 – 141G → A in AAB60675 (PubMed:8188287).Curated
Sequence conflicti21 – 211S → P in AAB60675 (PubMed:8188287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33225 mRNA. Translation: AAA63296.1.
U02995, U02994 Genomic DNA. Translation: AAB60675.1.
X15617 mRNA. Translation: CAA33636.1.
PIRiA36438. A43279.
RefSeqiNP_034161.2. NM_010031.2.
XP_011236417.1. XM_011238115.1.
UniGeneiMm.425522.

Genome annotation databases

GeneIDi105242466.
13216.
KEGGimmu:105242466.
mmu:13216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33225 mRNA. Translation: AAA63296.1.
U02995, U02994 Genomic DNA. Translation: AAB60675.1.
X15617 mRNA. Translation: CAA33636.1.
PIRiA36438. A43279.
RefSeqiNP_034161.2. NM_010031.2.
XP_011236417.1. XM_011238115.1.
UniGeneiMm.425522.

3D structure databases

ProteinModelPortaliP11477.
SMRiP11477. Positions 62-93.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PeptideAtlasiP11477.
PRIDEiP11477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi105242466.
13216.
KEGGimmu:105242466.
mmu:13216.

Organism-specific databases

CTDi1667.
MGIiMGI:94880. Defa1.

Phylogenomic databases

HOGENOMiHOG000233351.
HOVERGENiHBG011703.
InParanoidiP11477.
KOiK05230.
PhylomeDBiP11477.

Miscellaneous databases

PROiP11477.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DEFA1.

Family and domain databases

InterProiIPR006081. Alpha-defensin.
IPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel mouse gene family coding for cationic, cysteine-rich peptides. Regulation in small intestine and cells of myeloid origin."
    Ouellette A.J., Lauldi J.C.
    J. Biol. Chem. 265:9831-9837(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss albino.
    Tissue: Small intestine.
  2. "Structure and diversity of the murine cryptdin gene family."
    Huttner K.M., Selsted M.E., Ouellette A.J.
    Genomics 19:448-453(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. "Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium."
    Ouellette A.J., Greco R.M., James M., Frederick D., Naftilan J., Fallon J.T.
    J. Cell Biol. 108:1687-1695(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Enteric defensins: antibiotic peptide components of intestinal host defense."
    Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J.
    J. Cell Biol. 118:929-936(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 59-93.
    Strain: SWR/J.
    Tissue: Small intestine.
  5. "Purification and primary structure of murine cryptdin-1, a Paneth cell defensin."
    Ouellette A.J., Miller S.I., Henschen A.H., Selsted M.E.
    FEBS Lett. 304:146-148(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 59-93.
    Strain: SWR/J.
    Tissue: Intestinal crypt.

Entry informationi

Entry nameiDEFA1_MOUSE
AccessioniPrimary (citable) accession number: P11477
Secondary accession number(s): Q61448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1990
Last modified: July 6, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.