P11470 (GERE_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spore germination protein GerE

Gene names

Name:	gerE
Ordered Locus Names:	BSU28410

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	74 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of spore formation. Directs the transcription of several genes that encode structural components of the protein coat that encases the mature spore (CotB, CotC, CotG, CotS, CotV, CotW, CotX, CotY and CotZ). Controls also the cgeAB and cgeCDE operons.
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Germination Transcription Transcription regulation
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 74

74

Spore germination protein GerE

PRO_0000184159

Regions

Domain

5 – 70

66

HTH luxR-type

DNA binding

29 – 48

20

H-T-H motif By similarity

Experimental info

Sequence conflict

11

1

L → S in AAA22473. Ref.1

Sequence conflict

11

1

L → S in CAA99550. Ref.2

Sequence conflict

11

1

L → S Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

74

Helix Strand Turn

Details...

Helix

14 – 23

10

Turn

24 – 26

3

Helix

29 – 36

8

Helix

40 – 54

15

Helix

59 – 68

10

Sequences

	Sequence		Length	Mass (Da)
	P11470 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 8EF9C6194D39556B	FASTA	74	8,588
	10 20 30 40 50 60 MKEKEFQSKP LLTKREREVF ELLVQDKTTK EIASELFISE KTVRNHISNA MQKLGVKGRS 70 QAVVELLRMG ELEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence and the transcription during sporulation of the gerE gene of Bacillus subtilis." Cutting S.M., Mandelstam J. J. Gen. Microbiol. 132:3013-3024(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"Crystallisation and preliminary X-ray diffraction analysis of the sporulation regulatory protein GerE from Bacillus subtilis." Ducros V., Brannigan J.A., Lewis R.J., Wilkinson A.J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / IG20.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 11.
[6]	"Regulatory studies on the promoter for a gene governing synthesis and assembly of the spore coat in Bacillus subtilis." Cutting S.M., Panzer S., Losick R. J. Mol. Biol. 207:393-404(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
[7]	"Crystal structure of GerE, the ultimate transcriptional regulator of spore formation in Bacillus subtilis." Ducros V.M.-A., Lewis R.J., Verma C.S., Dodson E.J., Leonard G., Turkenburg J.P., Murshudov G.N., Wilkinson A.J., Brannigan J.A. J. Mol. Biol. 306:759-771(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17642 Genomic DNA. Translation: AAA22473.1.
Z75208 Genomic DNA. Translation: CAA99550.1.
AJ223964 Genomic DNA. Translation: CAA11701.1.
AL009126 Genomic DNA. Translation: CAB14801.2.

PIR

C27893.

RefSeq

NP_390719.2. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FSE	X-ray	2.05	A/B/C/D/E/F	1-74	[»]

ProteinModelPortal

P11470.

SMR

P11470. Positions 5-74.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU28410.

Proteomic databases

PaxDb

P11470.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB14801; CAB14801; BSU28410.

GeneID

937462.

KEGG

bsu:BSU28410.

PATRIC

18977566. VBIBacSub10457_2971.

Organism-specific databases

GenoList

BSU28410. [Micado]

Phylogenomic databases

eggNOG

COG2771.

HOGENOM

HOG000097463.

KO

K01994.

ProtClustDB

CLSK873497.

Enzyme and pathway databases

BioCyc

BSUB:BSU28410-MONOMER.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00196. GerE. 1 hit.
[Graphical view]

PRINTS

PR00038. HTHLUXR.

SMART

SM00421. HTH_LUXR. 1 hit.
[Graphical view]

SUPFAM

SSF46894. Bipartite_resp_reg_C-effector. 1 hit.

PROSITE

PS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P11470.

Entry information

Entry name

GERE_BACSU

Accession

Primary (citable) accession number: P11470

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program