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Protein

Peroxisomal carnitine O-octanoyltransferase

Gene

Crot

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate.

Catalytic activityi

Octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei327 – 3271Proton acceptorBy similarity
Binding sitei406 – 4061Coenzyme ABy similarity
Binding sitei439 – 4391CarnitineBy similarity
Binding sitei441 – 4411Carnitine; via carbonyl oxygenBy similarity
Binding sitei452 – 4521CarnitineBy similarity

GO - Molecular functioni

  • carnitine O-octanoyltransferase activity Source: RGD

GO - Biological processi

  • carnitine metabolic process Source: UniProtKB
  • coenzyme A metabolic process Source: UniProtKB
  • fatty acid beta-oxidation Source: UniProtKB-UniPathway
  • fatty acid metabolic process Source: UniProtKB
  • generation of precursor metabolites and energy Source: UniProtKB
  • long-chain fatty acid transport Source: RGD
  • medium-chain fatty acid metabolic process Source: UniProtKB
  • medium-chain fatty acid transport Source: RGD
  • response to drug Source: RGD
  • response to organonitrogen compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism, Transport

Enzyme and pathway databases

BRENDAi2.3.1.137. 5301.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal carnitine O-octanoyltransferase (EC:2.3.1.137)
Short name:
COT
Gene namesi
Name:Crot
Synonyms:Cot
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70908. Crot.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi238 – 2381A → D: Reduces activity by 80%. No effect on inhibition by malonyl-coenzyme A. 1 Publication
Mutagenesisi327 – 3271H → A: Loss of activity. 1 Publication
Mutagenesisi553 – 5531G → M: Lowers activity towards medium and long chain fatty acids. Increases activity towards short chain fatty acids. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Peroxisomal carnitine O-octanoyltransferasePRO_0000210171Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei40 – 401N6-succinyllysineBy similarity
Modified residuei57 – 571N6-succinyllysineBy similarity
Modified residuei406 – 4061N6-acetyllysine; alternateBy similarity
Modified residuei406 – 4061N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP11466.
PRIDEiP11466.

PTM databases

iPTMnetiP11466.
PhosphoSiteiP11466.

Expressioni

Tissue specificityi

Liver.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063856.

Structurei

3D structure databases

ProteinModelPortaliP11466.
SMRiP11466. Positions 11-610.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni410 – 4178Coenzyme A bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi610 – 6123Microbody targeting signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi534 – 5374Poly-Gly

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3718. Eukaryota.
ENOG410ZE4S. LUCA.
HOGENOMiHOG000233845.
HOVERGENiHBG104403.
InParanoidiP11466.
KOiK05940.
PhylomeDBiP11466.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENQLAKSIE ERTFQYQDSL PPLPVPSLEE SLKKYLESVK PFANEDEYKK
60 70 80 90 100
TEEIVQKFQD GVGKTLHQKL LERAKGKRNW LEEWWLNVAY LDVRIPSQLN
110 120 130 140 150
VNFVGPSPHF EHYWPAREGT QLERGSILLW HNLNYWQLLR REKLPVHKSG
160 170 180 190 200
NTPLDMNQFR MLFSTCKVPG ITRDSIMNYF KTESEGHCPT HIAVLCRGRA
210 220 230 240 250
FVFDVLHDGC LITPPELLRQ LTYIYQKCWN EPVGPSIAAL TSEERTRWAK
260 270 280 290 300
AREYLIGLDP ENLTLLEKIQ SSLFVYSIED TSPHATPENF SQVFEMLLGG
310 320 330 340 350
DPAVRWGDKS YNLISFANGI FGCSCDHAPY DAMLMVNIAH YVDEKLLETE
360 370 380 390 400
GRWKGSEKVR DIPLPEELAF TVDEKILNDV YQAKAQHLKA ASDLQIAAST
410 420 430 440 450
FTSFGKKLTK KEALHPDTFI QLALQLAYYR LHGRPGCCYE TAMTRYFYHG
460 470 480 490 500
RTETVRSCTV EAVRWCQSMQ DPSASLLERQ QKMLDAFAKH NKMMRDCSHG
510 520 530 540 550
KGFDRHLLGL LLIAKEEGLP VPELFEDPLF SRSGGGGNFV LSTSLVGYLR
560 570 580 590 600
IQGVVVPMVH NGYGFFYHIR DDRFVVTCSS WRSCLETDAE KLVEMIFHAF
610
HDMIHLMNTA HL
Length:612
Mass (Da):70,302
Last modified:February 1, 1996 - v3
Checksum:i41B2F3474C8838D1
GO

Sequence cautioni

The sequence AAA40948.1 differs from that shown.Curated
The sequence AAA40948.1 differs from that shown. Reason: Frameshift at position 464. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti334 – 3341L → F in AAA40948 (PubMed:3233218).Curated
Sequence conflicti463 – 4642VR → RQ in AAA40948 (PubMed:3233218).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26033 mRNA. Translation: AAC52317.1.
J02844 mRNA. Translation: AAA40948.1. Sequence problems.
PIRiA31948.
S60025.
RefSeqiNP_114193.1. NM_031987.1.
UniGeneiRn.4896.

Genome annotation databases

GeneIDi83842.
KEGGirno:83842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U26033 mRNA. Translation: AAC52317.1.
J02844 mRNA. Translation: AAA40948.1. Sequence problems.
PIRiA31948.
S60025.
RefSeqiNP_114193.1. NM_031987.1.
UniGeneiRn.4896.

3D structure databases

ProteinModelPortaliP11466.
SMRiP11466. Positions 11-610.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063856.

PTM databases

iPTMnetiP11466.
PhosphoSiteiP11466.

Proteomic databases

PaxDbiP11466.
PRIDEiP11466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83842.
KEGGirno:83842.

Organism-specific databases

CTDi54677.
RGDi70908. Crot.

Phylogenomic databases

eggNOGiKOG3718. Eukaryota.
ENOG410ZE4S. LUCA.
HOGENOMiHOG000233845.
HOVERGENiHBG104403.
InParanoidiP11466.
KOiK05940.
PhylomeDBiP11466.

Enzyme and pathway databases

UniPathwayiUPA00659.
BRENDAi2.3.1.137. 5301.

Miscellaneous databases

PROiP11466.

Family and domain databases

InterProiIPR000542. Carn_acyl_trans.
[Graphical view]
PANTHERiPTHR22589. PTHR22589. 1 hit.
PfamiPF00755. Carn_acyltransf. 1 hit.
[Graphical view]
PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
PS00440. ACYLTRANSF_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and sequence analysis of the rat liver carnitine octanoyltransferase cDNA, its natural gene and the gene promoter."
    Choi S.J., Oh D.H., Song C.S., Roy A.K., Chatterjee B.
    Biochim. Biophys. Acta 1264:215-222(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Cloning, sequencing, and regulation of rat liver carnitine octanoyltransferase: transcriptional stimulation of the enzyme during peroxisome proliferation."
    Chatterjee B., Song C.S., Kim J.-M., Roy A.K.
    Biochemistry 27:9000-9006(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-547.
    Tissue: Liver.
  3. "Structural model of the catalytic core of carnitine palmitoyltransferase I and carnitine octanoyltransferase (COT): mutation of CPT I histidine 473 and alanine 381 and COT alanine 238 impairs the catalytic activity."
    Morillas M., Gomez-Puertas P., Roca R., Serra D., Asins G., Valencia A., Hegardt F.G.
    J. Biol. Chem. 276:45001-45008(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ALA-238 AND HIS-327, 3D-STRUCTURE MODELING.
  4. Cited for: MUTAGENESIS OF GLY-553, 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiOCTC_RAT
AccessioniPrimary (citable) accession number: P11466
Secondary accession number(s): P48033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.