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Protein

Quinolinate synthase A

Gene

nadA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.3 Publications

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.

Cofactori

[4Fe-4S] cluster2 PublicationsNote: Binds 1 [4Fe-4S] cluster per subunit.2 Publications

Enzyme regulationi

Activity is greater under oxic conditions and is regulated by a redox-active disulfide bond.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47IminoaspartateBy similarity1
Binding sitei68Iminoaspartate; via amide nitrogenBy similarity1
Binding sitei139IminoaspartateBy similarity1
Binding sitei156IminoaspartateBy similarity1
Binding sitei226IminoaspartateBy similarity1
Binding sitei243IminoaspartateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processPyridine nucleotide biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:QUINOLINATE-SYNTHA-MONOMER
MetaCyc:QUINOLINATE-SYNTHA-MONOMER
UniPathwayiUPA00253; UER00327

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase A (EC:2.5.1.72)
Gene namesi
Name:nadA
Synonyms:nicA
Ordered Locus Names:b0750, JW0733
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10630 nadA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi291C → S: Activity is 3.3-fold lower under oxic conditions than under anoxic conditions. 1 Publication1
Mutagenesisi294C → S: Activity is 13-fold lower under oxic conditions than under anoxic conditions. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001557601 – 347Quinolinate synthase AAdd BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi291 ↔ 294Redox-active

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP11458
PRIDEiP11458

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4263028, 14 interactors
DIPiDIP-1027N
IntActiP11458, 6 interactors
STRINGi316385.ECDH10B_0817

Structurei

3D structure databases

ProteinModelPortaliP11458
SMRiP11458
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105D0I Bacteria
COG0379 LUCA
HOGENOMiHOG000222769
InParanoidiP11458
KOiK03517
OMAiLWAPDRH
PhylomeDBiP11458

Family and domain databases

HAMAPiMF_00567 NadA_type1, 1 hit
InterProiView protein in InterPro
IPR003473 NadA
IPR036094 NadA_sf
IPR023513 Quinolinate_synth_A_type1
PANTHERiPTHR30573 PTHR30573, 1 hit
PfamiView protein in Pfam
PF02445 NadA, 1 hit
SUPFAMiSSF142754 SSF142754, 1 hit
TIGRFAMsiTIGR00550 nadA, 1 hit

Sequencei

Sequence statusi: Complete.

P11458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVMFDPDTA IYPFPPKPTP LSIDEKAYYR EKIKRLLKER NAVMVAHYYT
60 70 80 90 100
DPEIQQLAEE TGGCISDSLE MARFGAKHPA STLLVAGVRF MGETAKILSP
110 120 130 140 150
EKTILMPTLQ AECSLDLGCP VEEFNAFCDA HPDRTVVVYA NTSAAVKARA
160 170 180 190 200
DWVVTSSIAV ELIDHLDSLG EKIIWAPDKH LGRYVQKQTG GDILCWQGAC
210 220 230 240 250
IVHDEFKTQA LTRLQEEYPD AAILVHPESP QAIVDMADAV GSTSQLIAAA
260 270 280 290 300
KTLPHQRLIV ATDRGIFYKM QQAVPDKELL EAPTAGEGAT CRSCAHCPWM
310 320 330 340
AMNGLQAIAE ALEQEGSNHE VHVDERLRER ALVPLNRMLD FAATLRG
Length:347
Mass (Da):38,241
Last modified:November 1, 1997 - v3
Checksum:i17014F7621E5EC6B
GO

Sequence cautioni

The sequence CAA31216 differs from that shown. Reason: Frameshift at positions 12 and 304.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 36RL → LR in CAA31216 (PubMed:2841129).Curated2
Sequence conflicti153V → G in CAA31216 (PubMed:2841129).Curated1
Sequence conflicti170G → C in CAA31216 (PubMed:2841129).Curated1
Sequence conflicti329E → R in CAA31216 (PubMed:2841129).Curated1

Mass spectrometryi

Molecular mass is 38246 Da from positions 1 - 347. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12713 Genomic DNA Translation: CAA31216.1 Frameshift.
U00096 Genomic DNA Translation: AAC73837.1
AP009048 Genomic DNA Translation: BAA35409.1
PIRiF64810 SYECQA
RefSeqiNP_415271.1, NC_000913.3
WP_000115290.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73837; AAC73837; b0750
BAA35409; BAA35409; BAA35409
GeneIDi945351
KEGGiecj:JW0733
eco:b0750
PATRICifig|1411691.4.peg.1529

Similar proteinsi

Entry informationi

Entry nameiNADA_ECOLI
AccessioniPrimary (citable) accession number: P11458
Secondary accession number(s): P77373
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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