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P11456

- MPRD_BOVIN

UniProt

P11456 - MPRD_BOVIN

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Protein
Cation-dependent mannose-6-phosphate receptor
Gene
M6PR
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

GO - Molecular functioni

  1. mannose binding Source: InterPro
  2. mannose transmembrane transporter activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_205891. Lysosome Vesicle Biogenesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cation-dependent mannose-6-phosphate receptor
    Short name:
    CD Man-6-P receptor
    Short name:
    CD-MPR
    Alternative name(s):
    46 kDa mannose 6-phosphate receptor
    Short name:
    MPR 46
    Gene namesi
    Name:M6PR
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Lysosome membrane; Single-pass type I membrane protein 1 Publication

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini29 – 187159Lumenal Inferred
    Add
    BLAST
    Transmembranei188 – 21326Helical; Inferred
    Add
    BLAST
    Topological domaini214 – 27966Cytoplasmic Inferred
    Add
    BLAST

    GO - Cellular componenti

    1. Golgi apparatus Source: AgBase
    2. endosome Source: AgBase
    3. integral component of membrane Source: UniProtKB-KW
    4. late endosome Source: Ensembl
    5. lysosomal membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Lysosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi246 – 2516CRSKPR → AAAAAA: Leads to rapid lysosomal degradation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828
    Add
    BLAST
    Chaini29 – 279251Cation-dependent mannose-6-phosphate receptor
    PRO_0000019225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi59 – 591N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi85 – 851N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi96 – 961N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi109 – 1091N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi115 – 1151N-linked (GlcNAc...) Reviewed prediction
    Modified residuei269 – 2691Phosphoserine By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP11456.
    PRIDEiP11456.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with GGA1, GGA2 and GGA3 By similarity.1 Publication

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000024233.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi37 – 393
    Turni40 – 423
    Helixi45 – 539
    Helixi55 – 573
    Beta strandi62 – 665
    Beta strandi73 – 775
    Beta strandi79 – 813
    Beta strandi84 – 885
    Beta strandi90 – 967
    Turni97 – 993
    Beta strandi102 – 11514
    Beta strandi117 – 12610
    Beta strandi131 – 1333
    Helixi134 – 1363
    Beta strandi141 – 1477
    Beta strandi152 – 16211
    Beta strandi165 – 1673
    Beta strandi170 – 1778
    Helixi178 – 1803

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1C39X-ray1.85A/B31-182[»]
    1KEOX-ray2.20A/B29-182[»]
    1M6PX-ray1.80A/B31-182[»]
    2RL7X-ray2.00A/B/C/D29-182[»]
    2RL8X-ray1.45A/B29-182[»]
    2RL9X-ray2.40A/B29-182[»]
    2RLBX-ray1.75A/B29-182[»]
    3CY4X-ray1.51A/B29-182[»]
    3K41X-ray1.90A/B29-182[»]
    3K42X-ray2.30A/B29-182[»]
    3K43X-ray2.00A/B29-182[»]
    ProteinModelPortaliP11456.
    SMRiP11456. Positions 29-182.

    Miscellaneous databases

    EvolutionaryTraceiP11456.

    Family & Domainsi

    Domaini

    The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG45234.
    GeneTreeiENSGT00390000002109.
    HOGENOMiHOG000013085.
    HOVERGENiHBG006395.
    InParanoidiP11456.
    KOiK10089.
    OMAiSDWIMLI.
    OrthoDBiEOG7PP576.
    TreeFamiTF328910.

    Family and domain databases

    Gene3Di2.70.130.10. 1 hit.
    InterProiIPR028927. Man-6-P_rcpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR000296. Man_6_P_rcpt.
    [Graphical view]
    PfamiPF02157. Man-6-P_recep. 1 hit.
    [Graphical view]
    PRINTSiPR00715. MAN6PRECEPTR.
    SUPFAMiSSF50911. SSF50911. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11456-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMSPLHSSWR TGLLLLLLFS VAVRESWQTE EKTCDLVGEK GKESEKELAL    50
    LKRLTPLFNK SFESTVGQSP DMYSYVFRVC REAGNHSSGA GLVQINKSNG 100
    KETVVGRFNE TQIFNGSNWI MLIYKGGDEY DNHCGREQRR AVVMISCNRH 150
    TLADNFNPVS EERGKVQDCF YLFEMDSSLA CSPEISHLSV GSILLVTLAS 200
    LVAVYIIGGF LYQRLVVGAK GMEQFPHLAF WQDLGNLVAD GCDFVCRSKP 250
    RNVPAAYRGV GDDQLGEESE ERDDHLLPM 279
    Length:279
    Mass (Da):31,201
    Last modified:October 1, 1989 - v1
    Checksum:i7351C85A32F39A70
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17025 mRNA. Translation: AAA30634.1.
    BT029887 mRNA. Translation: ABM06137.1.
    BC120081 mRNA. Translation: AAI20082.1.
    PIRiA27068.
    RefSeqiNP_786973.1. NM_175779.3.
    UniGeneiBt.5032.

    Genome annotation databases

    EnsembliENSBTAT00000024233; ENSBTAP00000024233; ENSBTAG00000018207.
    GeneIDi281291.
    KEGGibta:281291.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M17025 mRNA. Translation: AAA30634.1 .
    BT029887 mRNA. Translation: ABM06137.1 .
    BC120081 mRNA. Translation: AAI20082.1 .
    PIRi A27068.
    RefSeqi NP_786973.1. NM_175779.3.
    UniGenei Bt.5032.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1C39 X-ray 1.85 A/B 31-182 [» ]
    1KEO X-ray 2.20 A/B 29-182 [» ]
    1M6P X-ray 1.80 A/B 31-182 [» ]
    2RL7 X-ray 2.00 A/B/C/D 29-182 [» ]
    2RL8 X-ray 1.45 A/B 29-182 [» ]
    2RL9 X-ray 2.40 A/B 29-182 [» ]
    2RLB X-ray 1.75 A/B 29-182 [» ]
    3CY4 X-ray 1.51 A/B 29-182 [» ]
    3K41 X-ray 1.90 A/B 29-182 [» ]
    3K42 X-ray 2.30 A/B 29-182 [» ]
    3K43 X-ray 2.00 A/B 29-182 [» ]
    ProteinModelPortali P11456.
    SMRi P11456. Positions 29-182.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000024233.

    Proteomic databases

    PaxDbi P11456.
    PRIDEi P11456.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000024233 ; ENSBTAP00000024233 ; ENSBTAG00000018207 .
    GeneIDi 281291.
    KEGGi bta:281291.

    Organism-specific databases

    CTDi 4074.

    Phylogenomic databases

    eggNOGi NOG45234.
    GeneTreei ENSGT00390000002109.
    HOGENOMi HOG000013085.
    HOVERGENi HBG006395.
    InParanoidi P11456.
    KOi K10089.
    OMAi SDWIMLI.
    OrthoDBi EOG7PP576.
    TreeFami TF328910.

    Enzyme and pathway databases

    Reactomei REACT_205891. Lysosome Vesicle Biogenesis.

    Miscellaneous databases

    EvolutionaryTracei P11456.
    NextBioi 20805321.

    Family and domain databases

    Gene3Di 2.70.130.10. 1 hit.
    InterProi IPR028927. Man-6-P_rcpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR000296. Man_6_P_rcpt.
    [Graphical view ]
    Pfami PF02157. Man-6-P_recep. 1 hit.
    [Graphical view ]
    PRINTSi PR00715. MAN6PRECEPTR.
    SUPFAMi SSF50911. SSF50911. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "46 kd mannose 6-phosphate receptor: cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor."
      Dahms N.M., Lobel P., Breitmeyer J., Chirgwin J.M., Kornfeld S.
      Cell 50:181-192(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal cerebellum.
    4. "The cation-dependent mannose 6-phosphate receptor. Structural requirements for mannose 6-phosphate binding and oligomerization."
      Dahms N.M., Kornfeld S.
      J. Biol. Chem. 264:11458-11467(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: HOMODIMERIZATION.
    5. "A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes."
      Rohrer J., Schweizer A., Johnson K.F., Kornfeld S.
      J. Cell Biol. 130:1297-1306(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF 246-CYS--ARG-251, SUBCELLULAR LOCATION.
    6. "Molecular basis of lysosomal enzyme recognition: three-dimensional structure of the cation-dependent mannose 6-phosphate receptor."
      Roberts D.L., Weix D.J., Dahms N.M., Kim J.-J.P.
      Cell 93:639-648(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 29-182.

    Entry informationi

    Entry nameiMPRD_BOVIN
    AccessioniPrimary (citable) accession number: P11456
    Secondary accession number(s): A1L5A0, Q0VCN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: September 3, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This receptor has optimal binding in the presence of divalent cations.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3

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