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P11456 (MPRD_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cation-dependent mannose-6-phosphate receptor

Short name=CD Man-6-P receptor
Short name=CD-MPR
Alternative name(s):
46 kDa mannose 6-phosphate receptor
Short name=MPR 46
Gene names
Name:M6PR
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

Subunit structure

Homodimer. Interacts with GGA1, GGA2 and GGA3 By similarity. Ref.4

Subcellular location

Lysosome membrane; Single-pass type I membrane protein Ref.5.

Domain

The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.

Miscellaneous

This receptor has optimal binding in the presence of divalent cations.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828
Chain29 – 279251Cation-dependent mannose-6-phosphate receptor
PRO_0000019225

Regions

Topological domain29 – 187159Lumenal Probable
Transmembrane188 – 21326Helical; Probable
Topological domain214 – 27966Cytoplasmic Probable

Amino acid modifications

Modified residue2691Phosphoserine By similarity
Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation1151N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis246 – 2516CRSKPR → AAAAAA: Leads to rapid lysosomal degradation. Ref.5

Secondary structure

................................... 279
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P11456 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 7351C85A32F39A70

FASTA27931,201
        10         20         30         40         50         60 
MMSPLHSSWR TGLLLLLLFS VAVRESWQTE EKTCDLVGEK GKESEKELAL LKRLTPLFNK 

        70         80         90        100        110        120 
SFESTVGQSP DMYSYVFRVC REAGNHSSGA GLVQINKSNG KETVVGRFNE TQIFNGSNWI 

       130        140        150        160        170        180 
MLIYKGGDEY DNHCGREQRR AVVMISCNRH TLADNFNPVS EERGKVQDCF YLFEMDSSLA 

       190        200        210        220        230        240 
CSPEISHLSV GSILLVTLAS LVAVYIIGGF LYQRLVVGAK GMEQFPHLAF WQDLGNLVAD 

       250        260        270 
GCDFVCRSKP RNVPAAYRGV GDDQLGEESE ERDDHLLPM 

« Hide

References

« Hide 'large scale' references
[1]"46 kd mannose 6-phosphate receptor: cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor."
Dahms N.M., Lobel P., Breitmeyer J., Chirgwin J.M., Kornfeld S.
Cell 50:181-192(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal cerebellum.
[4]"The cation-dependent mannose 6-phosphate receptor. Structural requirements for mannose 6-phosphate binding and oligomerization."
Dahms N.M., Kornfeld S.
J. Biol. Chem. 264:11458-11467(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION.
[5]"A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes."
Rohrer J., Schweizer A., Johnson K.F., Kornfeld S.
J. Cell Biol. 130:1297-1306(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF 246-CYS--ARG-251, SUBCELLULAR LOCATION.
[6]"Molecular basis of lysosomal enzyme recognition: three-dimensional structure of the cation-dependent mannose 6-phosphate receptor."
Roberts D.L., Weix D.J., Dahms N.M., Kim J.-J.P.
Cell 93:639-648(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 29-182.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M17025 mRNA. Translation: AAA30634.1.
BT029887 mRNA. Translation: ABM06137.1.
BC120081 mRNA. Translation: AAI20082.1.
PIRA27068.
RefSeqNP_786973.1. NM_175779.3.
UniGeneBt.5032.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1C39X-ray1.85A/B31-182[»]
1KEOX-ray2.20A/B29-182[»]
1M6PX-ray1.80A/B31-182[»]
2RL7X-ray2.00A/B/C/D29-182[»]
2RL8X-ray1.45A/B29-182[»]
2RL9X-ray2.40A/B29-182[»]
2RLBX-ray1.75A/B29-182[»]
3CY4X-ray1.51A/B29-182[»]
3K41X-ray1.90A/B29-182[»]
3K42X-ray2.30A/B29-182[»]
3K43X-ray2.00A/B29-182[»]
ProteinModelPortalP11456.
SMRP11456. Positions 29-182.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000024233.

Proteomic databases

PaxDbP11456.
PRIDEP11456.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000024233; ENSBTAP00000024233; ENSBTAG00000018207.
GeneID281291.
KEGGbta:281291.

Organism-specific databases

CTD4074.

Phylogenomic databases

eggNOGNOG45234.
GeneTreeENSGT00390000002109.
HOGENOMHOG000013085.
HOVERGENHBG006395.
InParanoidP11456.
KOK10089.
OMASDWIMLI.
OrthoDBEOG7PP576.
TreeFamTF328910.

Family and domain databases

Gene3D2.70.130.10. 1 hit.
InterProIPR028927. Man-6-P_rcpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR000296. Man_6_P_rcpt.
[Graphical view]
PfamPF02157. Man-6-P_recep. 1 hit.
[Graphical view]
PRINTSPR00715. MAN6PRECEPTR.
SUPFAMSSF50911. SSF50911. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP11456.
NextBio20805321.

Entry information

Entry nameMPRD_BOVIN
AccessionPrimary (citable) accession number: P11456
Secondary accession number(s): A1L5A0, Q0VCN7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references