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P11452 (CLCB_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloromuconate cycloisomerase

EC=5.5.1.7
Alternative name(s):
Muconate cycloisomerase II
Gene names
Name:clcB
Encoded onPlasmid pAC27
OrganismPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifier303 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Catalytic activity

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactor

Manganese By similarity.

Pathway

Aromatic compound metabolism; 3-chlorocatechol degradation.

Sequence similarities

Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Chloromuconate cycloisomerase
PRO_0000171253

Sites

Active site1651Proton acceptor By similarity
Active site3231Proton donor By similarity
Metal binding1941Manganese By similarity
Metal binding2201Manganese By similarity
Metal binding2451Manganese By similarity

Experimental info

Sequence conflict421V → C Ref.1
Sequence conflict421V → C Ref.2
Sequence conflict1141R → H Ref.1
Sequence conflict1141R → H Ref.2

Sequences

Sequence LengthMass (Da)Tools
P11452 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: 9279DD393F42BBCD

FASTA37039,847
        10         20         30         40         50         60 
MKIEAIDVTL VDVPASRPIQ MSFTTVQKQS YAIVQIRAGG LVGIGEGSSV GGPTWSSECA 

        70         80         90        100        110        120 
ETIKVIIETY LAPLLIGKDA TNLRELQHLM ERAVTGNYSA KAAIDVALHD LKARSLNLPL 

       130        140        150        160        170        180 
SDLIGGAIQQ GIPIAWTLAS GDTQRDIAIA EEMIERRRHN RFKIKLGVRS PADDLRHIEK 

       190        200        210        220        230        240 
IIERVGDRAA VRVDINQAWD ENTASVWIPR LEAAGVELVE QPVARSNFDA LRRLSADNGV 

       250        260        270        280        290        300 
AILADESLSS LASAFELARH HCVDAFSLKL CNMGGVANTL KVAAIAEASG IASYGGTMLD 

       310        320        330        340        350        360 
SSIGTAAALH VYATLPTMPF ECELLGPWVL ADTLTQTQLE IKDFEIRLPS GPGLGVDIDP 

       370 
DKLRHFTRAG 

« Hide

References

[1]"Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation."
Frantz B., Chakrabarty A.M.
Proc. Natl. Acad. Sci. U.S.A. 84:4460-4464(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and complete nucleotide sequence determination of the catB gene encoding cis,cis-muconate lactonizing enzyme."
Aldrich T.L., Frantz B., Gill J.F., Kilbane J.J., Chakrabarty A.M.
Gene 52:185-195(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27."
Ghosal D., You I.-S.
Gene 83:225-232(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AC867.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16964 Genomic DNA. Translation: AAA98282.1.
M31457 Genomic DNA. Translation: AAA98260.1.
PIRJQ0176.

3D structure databases

ProteinModelPortalP11452.
SMRP11452. Positions 1-369.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00083.

Family and domain databases

InterProIPR013370. Chloromuconate_cycloisomerase.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERPTHR13794. PTHR13794. 1 hit.
PTHR13794:SF12. PTHR13794:SF12. 1 hit.
PfamPF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTSM00922. MR_MLE. 1 hit.
[Graphical view]
TIGRFAMsTIGR02534. mucon_cyclo. 1 hit.
PROSITEPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCLCB_PSEPU
AccessionPrimary (citable) accession number: P11452
Secondary accession number(s): P15741
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1990
Last modified: October 16, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways