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P11452

- CLCB_PSEPU

UniProt

P11452 - CLCB_PSEPU

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Protein

Chloromuconate cycloisomerase

Gene

clcB

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Catalytic activityi

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactori

Manganese.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651Proton acceptorBy similarity
Metal bindingi194 – 1941ManganeseBy similarity
Metal bindingi220 – 2201ManganeseBy similarity
Metal bindingi245 – 2451ManganeseBy similarity
Active sitei323 – 3231Proton donorBy similarity

GO - Molecular functioni

  1. chloromuconate cycloisomerase activity Source: UniProtKB-EC
  2. manganese ion binding Source: InterPro
  3. muconate cycloisomerase activity Source: InterPro

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
  2. cellular amino acid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00083.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloromuconate cycloisomerase (EC:5.5.1.7)
Alternative name(s):
Muconate cycloisomerase II
Gene namesi
Name:clcB
Encoded oniPlasmid pAC270 Publication
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Chloromuconate cycloisomerasePRO_0000171253Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP11452.
SMRiP11452. Positions 1-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11452-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIEAIDVTL VDVPASRPIQ MSFTTVQKQS YAIVQIRAGG LVGIGEGSSV
60 70 80 90 100
GGPTWSSECA ETIKVIIETY LAPLLIGKDA TNLRELQHLM ERAVTGNYSA
110 120 130 140 150
KAAIDVALHD LKARSLNLPL SDLIGGAIQQ GIPIAWTLAS GDTQRDIAIA
160 170 180 190 200
EEMIERRRHN RFKIKLGVRS PADDLRHIEK IIERVGDRAA VRVDINQAWD
210 220 230 240 250
ENTASVWIPR LEAAGVELVE QPVARSNFDA LRRLSADNGV AILADESLSS
260 270 280 290 300
LASAFELARH HCVDAFSLKL CNMGGVANTL KVAAIAEASG IASYGGTMLD
310 320 330 340 350
SSIGTAAALH VYATLPTMPF ECELLGPWVL ADTLTQTQLE IKDFEIRLPS
360 370
GPGLGVDIDP DKLRHFTRAG
Length:370
Mass (Da):39,847
Last modified:August 1, 1990 - v2
Checksum:i9279DD393F42BBCD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421V → C(PubMed:3299368)Curated
Sequence conflicti42 – 421V → C(PubMed:3609743)Curated
Sequence conflicti114 – 1141R → H(PubMed:3299368)Curated
Sequence conflicti114 – 1141R → H(PubMed:3609743)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M16964 Genomic DNA. Translation: AAA98282.1.
M31457 Genomic DNA. Translation: AAA98260.1.
PIRiJQ0176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M16964 Genomic DNA. Translation: AAA98282.1 .
M31457 Genomic DNA. Translation: AAA98260.1 .
PIRi JQ0176.

3D structure databases

ProteinModelPortali P11452.
SMRi P11452. Positions 1-369.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00083 .

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProi IPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view ]
PANTHERi PTHR13794. PTHR13794. 1 hit.
Pfami PF02746. MR_MLE_N. 1 hit.
[Graphical view ]
SMARTi SM00922. MR_MLE. 1 hit.
[Graphical view ]
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsi TIGR02534. mucon_cyclo. 1 hit.
PROSITEi PS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation."
    Frantz B., Chakrabarty A.M.
    Proc. Natl. Acad. Sci. U.S.A. 84:4460-4464(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and complete nucleotide sequence determination of the catB gene encoding cis,cis-muconate lactonizing enzyme."
    Aldrich T.L., Frantz B., Gill J.F., Kilbane J.J., Chakrabarty A.M.
    Gene 52:185-195(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27."
    Ghosal D., You I.-S.
    Gene 83:225-232(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AC867.

Entry informationi

Entry nameiCLCB_PSEPU
AccessioniPrimary (citable) accession number: P11452
Secondary accession number(s): P15741
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3