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P11452

- CLCB_PSEPU

UniProt

P11452 - CLCB_PSEPU

Protein

Chloromuconate cycloisomerase

Gene

clcB

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

    Catalytic activityi

    2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei165 – 1651Proton acceptorBy similarity
    Metal bindingi194 – 1941ManganeseBy similarity
    Metal bindingi220 – 2201ManganeseBy similarity
    Metal bindingi245 – 2451ManganeseBy similarity
    Active sitei323 – 3231Proton donorBy similarity

    GO - Molecular functioni

    1. chloromuconate cycloisomerase activity Source: UniProtKB-EC
    2. manganese ion binding Source: InterPro
    3. muconate cycloisomerase activity Source: InterPro

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW
    2. cellular amino acid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00083.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chloromuconate cycloisomerase (EC:5.5.1.7)
    Alternative name(s):
    Muconate cycloisomerase II
    Gene namesi
    Name:clcB
    Encoded oniPlasmid pAC270 Publication
    OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
    Taxonomic identifieri303 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Chloromuconate cycloisomerasePRO_0000171253Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP11452.
    SMRiP11452. Positions 1-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR013370. Chloromuconate_cycloisomerase.
    IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
    PROSITEiPS00908. MR_MLE_1. 1 hit.
    PS00909. MR_MLE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11452-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIEAIDVTL VDVPASRPIQ MSFTTVQKQS YAIVQIRAGG LVGIGEGSSV    50
    GGPTWSSECA ETIKVIIETY LAPLLIGKDA TNLRELQHLM ERAVTGNYSA 100
    KAAIDVALHD LKARSLNLPL SDLIGGAIQQ GIPIAWTLAS GDTQRDIAIA 150
    EEMIERRRHN RFKIKLGVRS PADDLRHIEK IIERVGDRAA VRVDINQAWD 200
    ENTASVWIPR LEAAGVELVE QPVARSNFDA LRRLSADNGV AILADESLSS 250
    LASAFELARH HCVDAFSLKL CNMGGVANTL KVAAIAEASG IASYGGTMLD 300
    SSIGTAAALH VYATLPTMPF ECELLGPWVL ADTLTQTQLE IKDFEIRLPS 350
    GPGLGVDIDP DKLRHFTRAG 370
    Length:370
    Mass (Da):39,847
    Last modified:August 1, 1990 - v2
    Checksum:i9279DD393F42BBCD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti42 – 421V → C(PubMed:3299368)Curated
    Sequence conflicti42 – 421V → C(PubMed:3609743)Curated
    Sequence conflicti114 – 1141R → H(PubMed:3299368)Curated
    Sequence conflicti114 – 1141R → H(PubMed:3609743)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16964 Genomic DNA. Translation: AAA98282.1.
    M31457 Genomic DNA. Translation: AAA98260.1.
    PIRiJQ0176.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16964 Genomic DNA. Translation: AAA98282.1 .
    M31457 Genomic DNA. Translation: AAA98260.1 .
    PIRi JQ0176.

    3D structure databases

    ProteinModelPortali P11452.
    SMRi P11452. Positions 1-369.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00083 .

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProi IPR013370. Chloromuconate_cycloisomerase.
    IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view ]
    PANTHERi PTHR13794. PTHR13794. 1 hit.
    Pfami PF02746. MR_MLE_N. 1 hit.
    [Graphical view ]
    SMARTi SM00922. MR_MLE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR02534. mucon_cyclo. 1 hit.
    PROSITEi PS00908. MR_MLE_1. 1 hit.
    PS00909. MR_MLE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation."
      Frantz B., Chakrabarty A.M.
      Proc. Natl. Acad. Sci. U.S.A. 84:4460-4464(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning and complete nucleotide sequence determination of the catB gene encoding cis,cis-muconate lactonizing enzyme."
      Aldrich T.L., Frantz B., Gill J.F., Kilbane J.J., Chakrabarty A.M.
      Gene 52:185-195(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27."
      Ghosal D., You I.-S.
      Gene 83:225-232(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AC867.

    Entry informationi

    Entry nameiCLCB_PSEPU
    AccessioniPrimary (citable) accession number: P11452
    Secondary accession number(s): P15741
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3