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Reviewed, UniProtKB/Swiss-Prot P11447 (ARLY_ECOLI)

Last modified November 3, 2009. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Argininosuccinate lyase
      Short name=ASAL
    EC=4.3.2.1
Alternative name(s):
    Arginosuccinase
Gene names
Name: argH
Ordered Locus Names: b3960, JW3932
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

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Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process via ornithine

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionargininosuccinate lyase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457Argininosuccinate lyase HAMAP MF_00006
PRO_0000137768

Experimental info

Sequence conflict281R → G in AAB59147. Ref.5
Sequence conflict401W → R in AAB59147. Ref.5

Secondary structure

1 .............................................................. 457
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P11447-1 [UniParc].

Last modified October 1, 1993. Version 3.
Checksum: 0109EF31675B9717

FASTA45750,318
        10         20         30         40         50         60 
MALWGGRFTQ AADQRFKQFN DSLRFDYRLA EQDIVGSVAW SKALVTVGVL TAEEQAQLEE 

        70         80         90        100        110        120 
ALNVLLEDVR ARPQQILESD AEDIHSWVEG KLIDKVGQLG KKLHTGRSRN DQVATDLKLW 

       130        140        150        160        170        180 
CKDTVSELLT ANRQLQSALV ETAQNNQDAV MPGYTHLQRA QPVTFAHWCL AYVEMLARDE 

       190        200        210        220        230        240 
SRLQDALKRL DVSPLGCGAL AGTAYEIDRE QLAGWLGFAS ATRNSLDSVS DRDHVLELLS 

       250        260        270        280        290        300 
AAAIGMVHLS RFAEDLIFFN TGEAGFVELS DRVTSGSSLM PQKKNPDALE LIRGKCGRVQ 

       310        320        330        340        350        360 
GALTGMMMTL KGLPLAYNKD MQEDKEGLFD ALDTWLDCLH MAALVLDGIQ VKRPRCQEAA 

       370        380        390        400        410        420 
QQGYANATEL ADYLVAKGVP FREAHHIVGE AVVEAIRQGK PLEDLPLSEL QKFSQVIDED 

       430        440        450 
VYPILSLQSC LDKRAAKGGV SPQQVAQAIA FAQARLG

« Hide

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References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Nucleotide sequence of Escherichia coli argB and argC genes: comparison of N-acetylglutamate kinase and N-acetylglutamate-gamma-semialdehyde dehydrogenase with homologous and analogous enzymes."
Parsot C., Boyen A., Cohen G.N., Glansdorff N.
Gene 68:275-283(1988) [PubMed: 2851495] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51.
[5]"IS3 can function as a mobile promoter in E. coli."
Charlier D.R.M., Piette J., Glansdorff N.
Nucleic Acids Res. 10:5935-5948(1982) [PubMed: 6292860] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44.
Strain: K12.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U00006 Genomic DNA. Translation: AAC43066.1.
U00096 Genomic DNA. Translation: AAC76942.1.
AP009048 Genomic DNA. Translation: BAE77351.1.
M21446 Genomic DNA. Translation: AAA23479.1.
J01590 Genomic DNA. Translation: AAB59147.1.
PIRC65203.
RefSeqAP_003850.1.
NP_418395.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1TJ7X-ray2.44A/B1-457[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:9142N.
STRINGP11447.

Genome annotation databases

GeneID948463.
GenomeReviewsGene locus JW3932 in contig AP009048_GR.
Gene locus b3960 in contig U00096_GR.
KEGGecj:JW3932.
eco:b3960.

Organism-specific databases

EchoBASEEB1205.
EcoGeneEG11223. argH.
CMRSearch...

Phylogenomic databases

HOGENOMP11447.
OMAMAEDLIF.

Enzyme and pathway databases

BioCycEcoCyc:ARGSUCCINLYA-MON.
MetaCyc:ARGSUCCINLYA-MON.

Gene expression databases

GenevestigatorP11447.

Family and domain databases

HAMAPMF_00006.
[Tree]
InterProIPR009049. Argininosuccinate_lyase.
IPR003031. D_crystallin.
IPR000362. Fumarate_lyase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PANTHERPTHR11444:SF3. argH. 1 hit.
PfamPF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00145. ARGSUCLYASE.
PR00149. FUMRATELYASE.
TIGRFAMsTIGR00838. argH. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameARLY_ECOLI
AccessionPrimary (citable) accession number: P11447
Secondary accession number(s): Q2M8Q5, Q47060
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1993
Last modified: November 3, 2009
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents