Reviewed, UniProtKB/Swiss-Prot P11446 (ARGC_ECOLI)

Names and origin

Protein names

Recommended name:
    N-acetyl-gamma-glutamyl-phosphate reductase
      Short name=AGPR
    EC=1.2.1.38
Alternative name(s):
    N-acetyl-glutamate semialdehyde dehydrogenase
      Short name=NAGSA dehydrogenase

Gene names

Name:	argC
Ordered Locus Names:	b3958, JW3930

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH. HAMAP MF_00150
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4. HAMAP MF_00150
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	N-acetyl-gamma-glutamyl-phosphate reductase HAMAP MF_00150		PRO_0000112401
Sites
	Active site	154	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11446-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 67AC195ECE1C4789	FASTA	334	35,952
	10 20 30 40 50 60 MLNTLIVGAS GYAGAELVTY VNRHPHMNIT ALTVSAQSND AGKLISDLHP QLKGIVDLPL 70 80 90 100 110 120 QPMSDISEFS PGVDVVFLAT AHEVSHDLAP QFLEAGCVVF DLSGAFRVND ATFYEKYYGF 130 140 150 160 170 180 THQYPELLEQ AAYGLAEWCG NKLKEANLIA VPGCYPTAAQ LALKPLIDAD LLDLNQWPVI 190 200 210 220 230 240 NATSGVSGAG RKAAISNSFC EVSLQPYGVF THRHQPEIAT HLGADVIFTP HLGNFPRGIL 250 260 270 280 290 300 ETITCRLKSG VTQAQVAQVL QQAYAHKPLV RLYDKGVPAL KNVVGLPFCD IGFAVQGEHL 310 320 330 IIVATEDNLL KGAAAQAVQC ANIRFGYAET QSLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of Escherichia coli argB and argC genes: comparison of N-acetylglutamate kinase and N-acetylglutamate-gamma-semialdehyde dehydrogenase with homologous and analogous enzymes." Parsot C., Boyen A., Cohen G.N., Glansdorff N. Gene 68:275-283(1988) [PubMed: 2851495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The regulatory region of the divergent argECBH operon in Escherichia coli K-12." Piette J., Cunin R., Boyen A., Charlier D.R.M., Crabeel M., van Vliet F., Glansdorff N., Squires C., Squires C.L. Nucleic Acids Res. 10:8031-8048(1982) [PubMed: 6761650] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48. Strain: K12.
[6]	"Structural and biochemical characterization of the Escherichia coli argE gene product." Meinnel T., Schmitt E., Mechulam Y., Blanquet S. J. Bacteriol. 174:2323-2331(1992) [PubMed: 1551850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M21446 Genomic DNA. Translation: AAA23477.1. U00006 Genomic DNA. Translation: AAC43064.1. U00096 Genomic DNA. Translation: AAC76940.1. AP009048 Genomic DNA. Translation: BAE77353.1. J01587 Genomic DNA. Translation: AAB59146.1. X55417 Genomic DNA. No translation available.
PIR	RDECEP. JT0332.
RefSeq	AP_003852.1. NP_418393.1.
3D structure databases
SMR	P11446. Positions 1-334.
ModBase	Search...
Genome annotation databases
GeneID	948455.
GenomeReviews	Gene locus JW3930 in contig AP009048_GR. Gene locus b3958 in contig U00096_GR.
KEGG	ecj:JW3930. eco:b3958.
Organism-specific databases
EchoBASE	EB0063.
EcoGene	EG10065. argC.
CMR	Search...
Phylogenomic databases
HOGENOM	P11446.
OMA	P11446. VCRIAVH.
Enzyme and pathway databases
BioCyc	EcoCyc:N-ACETYLGLUTPREDUCT-MON. MetaCyc:N-ACETYLGLUTPREDUCT-MON.
Family and domain databases
HAMAP	MF_00150. [Tree]
InterPro	IPR000706. AGPR_act_site. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
ProDom	PD003765. AGPR_act_site. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01850. argC. 1 hit.
PROSITE	PS01224. ARGC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGC_ECOLI

Accession

Primary (citable) accession number: P11446
Secondary accession number(s): Q2M8Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)