Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-like protein 4A

Gene

UBL4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303).4 Publications

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL
  • ubiquitin-like protein transferase activity Source: ProtInc

GO - Biological processi

  • cellular protein modification process Source: ProtInc
  • tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
  • transport Source: UniProtKB-KW

Keywordsi

Biological processTransport

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein 4A
Alternative name(s):
Ubiquitin-like protein GDX
Gene namesi
Name:UBL4A
Synonyms:DXS254E, GDX, UBL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000102178.12.
HGNCiHGNC:12505. UBL4A.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48K → R: Loss of polyubiquitination by AMFR. 1 Publication1

Organism-specific databases

DisGeNETi8266.
OpenTargetsiENSG00000102178.
PharmGKBiPA37152.

Polymorphism and mutation databases

DMDMi136662.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001148641 – 157Ubiquitin-like protein 4AAdd BLAST157

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei90PhosphoserineCombined sources1

Post-translational modificationi

Polyubiquitinated. Ubiquitination by AMFR and deubiquitination by USP13 may regulate the interaction between the BAG6/BAT3 complex and SGTA and therefore may regulate client proteins fate.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP11441.
MaxQBiP11441.
PaxDbiP11441.
PeptideAtlasiP11441.
PRIDEiP11441.

PTM databases

iPTMnetiP11441.
PhosphoSitePlusiP11441.

Expressioni

Gene expression databases

BgeeiENSG00000102178.
CleanExiHS_UBL4A.
ExpressionAtlasiP11441. baseline and differential.
GenevisibleiP11441. HS.

Organism-specific databases

HPAiHPA003617.

Interactioni

Subunit structurei

Component of the BAG6/BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35 (PubMed:20676083). Interacts with USP13; may be indirect via BAG6 (PubMed:24424410).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi113885. 308 interactors.
IntActiP11441. 50 interactors.
MINTiMINT-1147351.
STRINGi9606.ENSP00000358674.

Structurei

Secondary structure

1157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 7Combined sources7
Beta strandi12 – 17Combined sources6
Helixi23 – 33Combined sources11
Turni38 – 40Combined sources3
Beta strandi42 – 45Combined sources4
Helixi56 – 59Combined sources4
Beta strandi63 – 65Combined sources3
Beta strandi68 – 70Combined sources3
Helixi95 – 106Combined sources12
Helixi109 – 129Combined sources21
Helixi132 – 142Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DZINMR-A1-74[»]
4WWRX-ray2.00B/D/F/H93-139[»]
4X86X-ray1.85A95-147[»]
ProteinModelPortaliP11441.
SMRiP11441.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11441.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76

Phylogenomic databases

eggNOGiKOG0001. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00730000111022.
HOGENOMiHOG000006827.
HOVERGENiHBG072205.
InParanoidiP11441.
OMAiYPGIKDG.
PhylomeDBiP11441.
TreeFamiTF354228.

Family and domain databases

InterProiView protein in InterPro
IPR019956. Ubiquitin.
IPR029071. Ubiquitin-like_domsf.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
PfamiView protein in Pfam
PF00240. ubiquitin. 1 hit.
PRINTSiPR00348. UBIQUITIN.
SMARTiView protein in SMART
SM00213. UBQ. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P11441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLTVKALQG RECSLQVPED ELVSTLKQLV SEKLNVPVRQ QRLLFKGKAL
60 70 80 90 100
ADGKRLSDYS IGPNSKLNLV VKPLEKVLLE EGEAQRLADS PPPQVWQLIS
110 120 130 140 150
KVLARHFSAA DASRVLEQLQ RDYERSLSRL TLDDIERLAS RFLHPEVTET

MEKGFSK
Length:157
Mass (Da):17,777
Last modified:October 1, 1989 - v1
Checksum:i9D6EE2D20D2C4C60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03589 Genomic DNA. Translation: AAA36790.1.
L44140 Genomic DNA. Translation: AAA92650.1.
BX664739 Genomic DNA. No translation available.
CH471172 Genomic DNA. Translation: EAW72700.1.
BC043346 mRNA. Translation: AAH43346.1.
BC053589 mRNA. Translation: AAH53589.1.
CCDSiCCDS14754.1.
PIRiA31084.
RefSeqiNP_055050.1. NM_014235.4.
UniGeneiHs.76480.

Genome annotation databases

EnsembliENST00000369660; ENSP00000358674; ENSG00000102178.
GeneIDi8266.
KEGGihsa:8266.
UCSCiuc004flo.4. human.

Similar proteinsi

Entry informationi

Entry nameiUBL4A_HUMAN
AccessioniPrimary (citable) accession number: P11441
Secondary accession number(s): Q5HY80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 22, 2017
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references