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P11412 (G6PD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase

Short name=G6PD
EC=1.1.1.49
Gene names
Name:ZWF1
Synonyms:MET19
Ordered Locus Names:YNL241C
ORF Names:N1110
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis By similarity. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Miscellaneous

Present with 15000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7 Ref.9
Chain2 – 505504Glucose-6-phosphate 1-dehydrogenase HAMAP-Rule MF_00966
PRO_0000068107

Regions

Nucleotide binding18 – 258NADP By similarity
Region187 – 1915Substrate binding By similarity

Sites

Active site2491Proton acceptor By similarity
Binding site521NADP By similarity
Binding site1571NADP; via carbonyl oxygen By similarity
Binding site1571Substrate By similarity
Binding site2251Substrate By similarity
Binding site2441Substrate By similarity
Binding site3431Substrate By similarity
Binding site3771Substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine Ref.7 Ref.9
Modified residue1421Phosphoserine Ref.11
Modified residue1451Phosphotyrosine Ref.11

Experimental info

Sequence conflict591Missing AA sequence Ref.7
Sequence conflict801K → N in AAT93017. Ref.6
Sequence conflict1751P → A in AAA34619. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P11412 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 9FC23E6CE599454E

FASTA50557,522
        10         20         30         40         50         60 
MSEGPVKFEK NTVISVFGAS GDLAKKKTFP ALFGLFREGY LDPSTKIFGY ARSKLSMEED 

        70         80         90        100        110        120 
LKSRVLPHLK KPHGEADDSK VEQFFKMVSY ISGNYDTDEG FDELRTQIEK FEKSANVDVP 

       130        140        150        160        170        180 
HRLFYLALPP SVFLTVAKQI KSRVYAENGI TRVIVEKPFG HDLASARELQ KNLGPLFKEE 

       190        200        210        220        230        240 
ELYRIDHYLG KELVKNLLVL RFGNQFLNAS WNRDNIQSVQ ISFKERFGTE GRGGYFDSIG 

       250        260        270        280        290        300 
IIRDVMQNHL LQIMTLLTME RPVSFDPESI RDEKVKVLKA VAPIDTDDVL LGQYGKSEDG 

       310        320        330        340        350        360 
SKPAYVDDDT VDKDSKCVTF AAMTFNIENE RWEGVPIMMR AGKALNESKV EIRLQYKAVA 

       370        380        390        400        410        420 
SGVFKDIPNN ELVIRVQPDA AVYLKFNAKT PGLSNATQVT DLNLTYASRY QDFWIPEAYE 

       430        440        450        460        470        480 
VLIRDALLGD HSNFVRDDEL DISWGIFTPL LKHIERPDGP TPEIYPYGSR GPKGLKEYMQ 

       490        500 
KHKYVMPEKH PYAWPVTKPE DTKDN 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase."
Nogae I., Johnston M.
Gene 96:161-169(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur."
Thomas D., Cherest H., Surdin-Kerjan Y.
EMBO J. 10:547-553(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"Functionally important regions of glucose-6-phosphate dehydrogenase defined by the Saccharomyces cerevisiae enzyme and its differences from the mammalian and insect forms."
Persson B., Joernvall H., Wood I., Jeffery J.
Eur. J. Biochem. 198:485-491(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-505, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
[8]"Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae: characterization of a reactive lysine residue labeled with acetylsalicylic acid."
Jeffery J., Hobbs L., Joernvall H.
Biochemistry 24:666-671(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 185-195.
[9]"Fast atom bombardment mass spectrometry and chemical analysis in determinations of acyl-blocked protein structures."
Egestad B., Estonius M., Danielsson O., Persson B., Cederlund E., Kaiser R., Holmquist B., Vallee B., Pares X., Jefferey J., Joernvall H.
FEBS Lett. 269:194-196(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-7, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND TYR-145, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34709 Genomic DNA. Translation: AAA34619.1.
X57336 Genomic DNA. Translation: CAA40611.1.
Z69381 Genomic DNA. Translation: CAA93357.1.
Z71517 Genomic DNA. Translation: CAA96146.1.
AY692998 Genomic DNA. Translation: AAT93017.1.
BK006947 Genomic DNA. Translation: DAA10318.1.
PIRS13744.
RefSeqNP_014158.1. NM_001183079.1.

3D structure databases

ProteinModelPortalP11412.
SMRP11412. Positions 14-484.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35598. 41 interactions.
DIPDIP-5061N.
IntActP11412. 4 interactions.
MINTMINT-501936.
STRING4932.YNL241C.

Chemistry

ChEMBLCHEMBL1075249.

2D gel databases

SWISS-2DPAGEP11412.

Proteomic databases

MaxQBP11412.
PaxDbP11412.
PeptideAtlasP11412.
PRIDEP11412.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL241C; YNL241C; YNL241C.
GeneID855480.
KEGGsce:YNL241C.

Organism-specific databases

SGDS000005185. ZWF1.

Phylogenomic databases

eggNOGCOG0364.
GeneTreeENSGT00530000063435.
HOGENOMHOG000046192.
KOK00036.
OMAYCVTAVS.
OrthoDBEOG7TXKRP.

Enzyme and pathway databases

BioCycMetaCyc:YNL241C-MONOMER.
YEAST:YNL241C-MONOMER.
SABIO-RKP11412.
UniPathwayUPA00115; UER00408.

Gene expression databases

GenevestigatorP11412.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979445.
PROP11412.

Entry information

Entry nameG6PD_YEAST
AccessionPrimary (citable) accession number: P11412
Secondary accession number(s): D6W0V2, E9P908
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 137 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways