Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

ZWF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Miscellaneous

Present with 15000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (ZWF1)
  2. 6-phosphogluconolactonase 3 (SOL3), 6-phosphogluconolactonase 4 (SOL4)
  3. 6-phosphogluconate dehydrogenase, decarboxylating 2 (GND2), 6-phosphogluconate dehydrogenase, decarboxylating 1 (GND1)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52NADPBy similarity1
Binding sitei157NADP; via carbonyl oxygenBy similarity1
Binding sitei157SubstrateBy similarity1
Binding sitei225SubstrateBy similarity1
Binding sitei244SubstrateBy similarity1
Active sitei249Proton acceptorBy similarity1
Binding sitei343SubstrateBy similarity1
Binding sitei377SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 25NADPBy similarity8

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: SGD
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: UniProtKB-KW
  • NADPH regeneration Source: SGD
  • pentose-phosphate shunt, oxidative branch Source: SGD
  • response to hydrogen peroxide Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:YNL241C-MONOMER
YEAST:YNL241C-MONOMER
ReactomeiR-SCE-5628897 TP53 Regulates Metabolic Genes
R-SCE-71336 Pentose phosphate pathway (hexose monophosphate shunt)
SABIO-RKP11412
UniPathwayiUPA00115; UER00408

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:ZWF1
Synonyms:MET19
Ordered Locus Names:YNL241C
ORF Names:N1110
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL241C
SGDiS000005185 ZWF1

Pathology & Biotechi

Disruption phenotypei

Shows growth defects in acetate-supplemented medium; the phenotype is enhanced by addition of hydrogen peroxide (increased oxidative stress) and/or double knockout of ZWF1 and YHM2. The cytosolic NADPH/NADP+ ratio is decreased compared to wild type; this effect is enhanced in the presence of hydrogen peroxide. Double knockouts of ZWF1 and YHM2 show an increased mitochondrial NADPH/NADP+ ratio, but only in the presence of hydrogen peroxide. Levels of cytosolic citrate are reduced, but only in the presence of hydrogen peroxide. Levels of cytosolic oxoglutarate are reduced, but only in the presence of hydrogen peroxide. Cells show a moderate increase in levels of reactive oxygen species (ROS) following hydrogen peroxide treatment; in double knockouts of YHM2 and ZWF1 there is a larger increase in ROS.1 Publication

Chemistry databases

ChEMBLiCHEMBL1075249

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000681072 – 505Glucose-6-phosphate 1-dehydrogenaseAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine2 Publications1
Modified residuei142PhosphoserineCombined sources1
Modified residuei145PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP11412
PaxDbiP11412
PRIDEiP11412

2D gel databases

SWISS-2DPAGEP11412

PTM databases

iPTMnetiP11412

Interactioni

Protein-protein interaction databases

BioGridi35598, 238 interactors
DIPiDIP-5061N
IntActiP11412, 4 interactors
MINTiP11412
STRINGi4932.YNL241C

Chemistry databases

BindingDBiP11412

Structurei

3D structure databases

ProteinModelPortaliP11412
SMRiP11412
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 191Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063435
HOGENOMiHOG000046192
InParanoidiP11412
KOiK00036
OMAiVEICVYE
OrthoDBiEOG092C2MOH

Family and domain databases

HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PIRSFiPIRSF000110 G6PD, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00871 zwf, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11412-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEGPVKFEK NTVISVFGAS GDLAKKKTFP ALFGLFREGY LDPSTKIFGY
60 70 80 90 100
ARSKLSMEED LKSRVLPHLK KPHGEADDSK VEQFFKMVSY ISGNYDTDEG
110 120 130 140 150
FDELRTQIEK FEKSANVDVP HRLFYLALPP SVFLTVAKQI KSRVYAENGI
160 170 180 190 200
TRVIVEKPFG HDLASARELQ KNLGPLFKEE ELYRIDHYLG KELVKNLLVL
210 220 230 240 250
RFGNQFLNAS WNRDNIQSVQ ISFKERFGTE GRGGYFDSIG IIRDVMQNHL
260 270 280 290 300
LQIMTLLTME RPVSFDPESI RDEKVKVLKA VAPIDTDDVL LGQYGKSEDG
310 320 330 340 350
SKPAYVDDDT VDKDSKCVTF AAMTFNIENE RWEGVPIMMR AGKALNESKV
360 370 380 390 400
EIRLQYKAVA SGVFKDIPNN ELVIRVQPDA AVYLKFNAKT PGLSNATQVT
410 420 430 440 450
DLNLTYASRY QDFWIPEAYE VLIRDALLGD HSNFVRDDEL DISWGIFTPL
460 470 480 490 500
LKHIERPDGP TPEIYPYGSR GPKGLKEYMQ KHKYVMPEKH PYAWPVTKPE

DTKDN
Length:505
Mass (Da):57,522
Last modified:January 23, 2007 - v4
Checksum:i9FC23E6CE599454E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59Missing AA sequence (PubMed:2040308).Curated1
Sequence conflicti80K → N in AAT93017 (PubMed:17322287).Curated1
Sequence conflicti175P → A in AAA34619 (PubMed:2269430).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34709 Genomic DNA Translation: AAA34619.1
X57336 Genomic DNA Translation: CAA40611.1
Z69381 Genomic DNA Translation: CAA93357.1
Z71517 Genomic DNA Translation: CAA96146.1
AY692998 Genomic DNA Translation: AAT93017.1
BK006947 Genomic DNA Translation: DAA10318.1
PIRiS13744
RefSeqiNP_014158.1, NM_001183079.1

Genome annotation databases

EnsemblFungiiYNL241C; YNL241C; YNL241C
GeneIDi855480
KEGGisce:YNL241C

Similar proteinsi

Entry informationi

Entry nameiG6PD_YEAST
AccessioniPrimary (citable) accession number: P11412
Secondary accession number(s): D6W0V2, E9P908
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 169 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health