P11412 (G6PD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphate 1-dehydrogenase
Short name=G6PD
EC=1.1.1.49

Gene names

Name:	ZWF1
Synonyms:	MET19
Ordered Locus Names:	YNL241C
ORF Names:	N1110

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Miscellaneous	Present with 15000 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	pentose-phosphate shunt, oxidative branch Inferred from mutant phenotype. Source: SGD response to hydrogen peroxide Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from mutant phenotype Ref.2Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-7233,EBI-7233

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7 Ref.9
	Chain	2 – 505	504	Glucose-6-phosphate 1-dehydrogenase		PRO_0000068107
Sites
	Active site	249	1	Proton acceptor By similarity
	Binding site	20	1	NADP By similarity
	Binding site	52	1	NADP By similarity
	Binding site	187	1	Substrate By similarity
	Binding site	191	1	Substrate
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.7
	Modified residue	142	1	Phosphoserine Ref.11
	Modified residue	145	1	Phosphotyrosine Ref.11
Experimental info
	Sequence conflict	59	1	Missing AA sequence Ref.7
	Sequence conflict	80	1	K → N in AAT93017. Ref.6
	Sequence conflict	175	1	P → A in AAA34619. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P11412 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 9FC23E6CE599454E	FASTA	505	57,522
	10 20 30 40 50 60 MSEGPVKFEK NTVISVFGAS GDLAKKKTFP ALFGLFREGY LDPSTKIFGY ARSKLSMEED 70 80 90 100 110 120 LKSRVLPHLK KPHGEADDSK VEQFFKMVSY ISGNYDTDEG FDELRTQIEK FEKSANVDVP 130 140 150 160 170 180 HRLFYLALPP SVFLTVAKQI KSRVYAENGI TRVIVEKPFG HDLASARELQ KNLGPLFKEE 190 200 210 220 230 240 ELYRIDHYLG KELVKNLLVL RFGNQFLNAS WNRDNIQSVQ ISFKERFGTE GRGGYFDSIG 250 260 270 280 290 300 IIRDVMQNHL LQIMTLLTME RPVSFDPESI RDEKVKVLKA VAPIDTDDVL LGQYGKSEDG 310 320 330 340 350 360 SKPAYVDDDT VDKDSKCVTF AAMTFNIENE RWEGVPIMMR AGKALNESKV EIRLQYKAVA 370 380 390 400 410 420 SGVFKDIPNN ELVIRVQPDA AVYLKFNAKT PGLSNATQVT DLNLTYASRY QDFWIPEAYE 430 440 450 460 470 480 VLIRDALLGD HSNFVRDDEL DISWGIFTPL LKHIERPDGP TPEIYPYGSR GPKGLKEYMQ 490 500 KHKYVMPEKH PYAWPVTKPE DTKDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase." Nogae I., Johnston M. Gene 96:161-169(1990) [PubMed: 2269430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur." Thomas D., Cherest H., Surdin-Kerjan Y. EMBO J. 10:547-553(1991) [PubMed: 2001672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain." Pandolfo D., de Antoni A., Lanfranchi G., Valle G. Yeast 12:1071-1076(1996) [PubMed: 8896273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Functionally important regions of glucose-6-phosphate dehydrogenase defined by the Saccharomyces cerevisiae enzyme and its differences from the mammalian and insect forms." Persson B., Joernvall H., Wood I., Jeffery J. Eur. J. Biochem. 198:485-491(1991) [PubMed: 2040308] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-505.
[8]	"Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae: characterization of a reactive lysine residue labeled with acetylsalicylic acid." Jeffery J., Hobbs L., Joernvall H. Biochemistry 24:666-671(1985) [PubMed: 3922403] [Abstract] Cited for: PROTEIN SEQUENCE OF 185-195.
[9]	"Fast atom bombardment mass spectrometry and chemical analysis in determinations of acyl-blocked protein structures." Egestad B., Estonius M., Danielsson O., Persson B., Cederlund E., Kaiser R., Holmquist B., Vallee B., Pares X., Jefferey J., Joernvall H. FEBS Lett. 269:194-196(1990) [PubMed: 2387402] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-7.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND TYR-145, MASS SPECTROMETRY. Strain: ADR376.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34709 Genomic DNA. Translation: AAA34619.1. X57336 Genomic DNA. Translation: CAA40611.1. Z69381 Genomic DNA. Translation: CAA93357.1. Z71517 Genomic DNA. Translation: CAA96146.1. AY692998 Genomic DNA. Translation: AAT93017.1. BK006947 Genomic DNA. Translation: DAA10318.1.
PIR	S13744.
RefSeq	NP_014158.1. NM_001183079.1.
3D structure databases
ProteinModelPortal	P11412.
SMR	P11412. Positions 2-493.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5061N.
IntAct	P11412. 6 interactions.
MINT	MINT-501936.
STRING	P11412.
2D gel databases
SWISS-2DPAGE	P11412.
Proteomic databases
PeptideAtlas	P11412.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL241C; YNL241C; YNL241C.
GeneID	855480.
KEGG	sce:YNL241C.
NMPDR	fig|4932.3.peg.5223.
Organism-specific databases
SGD	S000005185. ZWF1.
Phylogenomic databases
eggNOG	fuNOG04834.
HOGENOM	HBG322449.
OMA	PNWKEEE.
OrthoDB	EOG4RBTSW.
Gene expression databases
ArrayExpress	P11412.
Genevestigator	P11412.
GermOnline	YNL241C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001282. G6P_DH. IPR019796. G6P_DH_AS. IPR022675. G6P_DH_C. IPR022674. G6P_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00036.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. Zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979445.

Entry information

Entry name

G6PD_YEAST

Accession

Primary (citable) accession number: P11412
Secondary accession number(s): D6W0V2, E9P908

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 114 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program