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P11412

- G6PD_YEAST

UniProt

P11412 - G6PD_YEAST

Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

ZWF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis By similarity.By similarity

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521NADPBy similarity
    Binding sitei157 – 1571NADP; via carbonyl oxygenBy similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Binding sitei225 – 2251SubstrateBy similarity
    Binding sitei244 – 2441SubstrateBy similarity
    Active sitei249 – 2491Proton acceptorBy similarity
    Binding sitei343 – 3431SubstrateBy similarity
    Binding sitei377 – 3771SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 258NADPBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: SGD
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. NADPH regeneration Source: SGD
    2. pentose-phosphate shunt, oxidative branch Source: SGD
    3. response to hydrogen peroxide Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:YNL241C-MONOMER.
    YEAST:YNL241C-MONOMER.
    SABIO-RKP11412.
    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
    Short name:
    G6PD
    Gene namesi
    Name:ZWF1
    Synonyms:MET19
    Ordered Locus Names:YNL241C
    ORF Names:N1110
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    SGDiS000005185. ZWF1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 505504Glucose-6-phosphate 1-dehydrogenasePRO_0000068107Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei142 – 1421Phosphoserine1 Publication
    Modified residuei145 – 1451Phosphotyrosine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP11412.
    PaxDbiP11412.
    PeptideAtlasiP11412.
    PRIDEiP11412.

    2D gel databases

    SWISS-2DPAGEP11412.

    Expressioni

    Gene expression databases

    GenevestigatoriP11412.

    Interactioni

    Protein-protein interaction databases

    BioGridi35598. 41 interactions.
    DIPiDIP-5061N.
    IntActiP11412. 4 interactions.
    MINTiMINT-501936.
    STRINGi4932.YNL241C.

    Structurei

    3D structure databases

    ProteinModelPortaliP11412.
    SMRiP11412. Positions 14-484.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni187 – 1915Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0364.
    GeneTreeiENSGT00530000063435.
    HOGENOMiHOG000046192.
    KOiK00036.
    OMAiYCVTAVS.
    OrthoDBiEOG7TXKRP.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11412-1 [UniParc]FASTAAdd to Basket

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    MSEGPVKFEK NTVISVFGAS GDLAKKKTFP ALFGLFREGY LDPSTKIFGY    50
    ARSKLSMEED LKSRVLPHLK KPHGEADDSK VEQFFKMVSY ISGNYDTDEG 100
    FDELRTQIEK FEKSANVDVP HRLFYLALPP SVFLTVAKQI KSRVYAENGI 150
    TRVIVEKPFG HDLASARELQ KNLGPLFKEE ELYRIDHYLG KELVKNLLVL 200
    RFGNQFLNAS WNRDNIQSVQ ISFKERFGTE GRGGYFDSIG IIRDVMQNHL 250
    LQIMTLLTME RPVSFDPESI RDEKVKVLKA VAPIDTDDVL LGQYGKSEDG 300
    SKPAYVDDDT VDKDSKCVTF AAMTFNIENE RWEGVPIMMR AGKALNESKV 350
    EIRLQYKAVA SGVFKDIPNN ELVIRVQPDA AVYLKFNAKT PGLSNATQVT 400
    DLNLTYASRY QDFWIPEAYE VLIRDALLGD HSNFVRDDEL DISWGIFTPL 450
    LKHIERPDGP TPEIYPYGSR GPKGLKEYMQ KHKYVMPEKH PYAWPVTKPE 500
    DTKDN 505
    Length:505
    Mass (Da):57,522
    Last modified:January 23, 2007 - v4
    Checksum:i9FC23E6CE599454E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti59 – 591Missing AA sequence (PubMed:2040308)Curated
    Sequence conflicti80 – 801K → N in AAT93017. (PubMed:17322287)Curated
    Sequence conflicti175 – 1751P → A in AAA34619. (PubMed:2269430)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34709 Genomic DNA. Translation: AAA34619.1.
    X57336 Genomic DNA. Translation: CAA40611.1.
    Z69381 Genomic DNA. Translation: CAA93357.1.
    Z71517 Genomic DNA. Translation: CAA96146.1.
    AY692998 Genomic DNA. Translation: AAT93017.1.
    BK006947 Genomic DNA. Translation: DAA10318.1.
    PIRiS13744.
    RefSeqiNP_014158.1. NM_001183079.1.

    Genome annotation databases

    EnsemblFungiiYNL241C; YNL241C; YNL241C.
    GeneIDi855480.
    KEGGisce:YNL241C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34709 Genomic DNA. Translation: AAA34619.1 .
    X57336 Genomic DNA. Translation: CAA40611.1 .
    Z69381 Genomic DNA. Translation: CAA93357.1 .
    Z71517 Genomic DNA. Translation: CAA96146.1 .
    AY692998 Genomic DNA. Translation: AAT93017.1 .
    BK006947 Genomic DNA. Translation: DAA10318.1 .
    PIRi S13744.
    RefSeqi NP_014158.1. NM_001183079.1.

    3D structure databases

    ProteinModelPortali P11412.
    SMRi P11412. Positions 14-484.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35598. 41 interactions.
    DIPi DIP-5061N.
    IntActi P11412. 4 interactions.
    MINTi MINT-501936.
    STRINGi 4932.YNL241C.

    Chemistry

    ChEMBLi CHEMBL1075249.

    2D gel databases

    SWISS-2DPAGE P11412.

    Proteomic databases

    MaxQBi P11412.
    PaxDbi P11412.
    PeptideAtlasi P11412.
    PRIDEi P11412.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL241C ; YNL241C ; YNL241C .
    GeneIDi 855480.
    KEGGi sce:YNL241C.

    Organism-specific databases

    SGDi S000005185. ZWF1.

    Phylogenomic databases

    eggNOGi COG0364.
    GeneTreei ENSGT00530000063435.
    HOGENOMi HOG000046192.
    KOi K00036.
    OMAi YCVTAVS.
    OrthoDBi EOG7TXKRP.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .
    BioCyci MetaCyc:YNL241C-MONOMER.
    YEAST:YNL241C-MONOMER.
    SABIO-RK P11412.

    Miscellaneous databases

    NextBioi 979445.
    PROi P11412.

    Gene expression databases

    Genevestigatori P11412.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase."
      Nogae I., Johnston M.
      Gene 96:161-169(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur."
      Thomas D., Cherest H., Surdin-Kerjan Y.
      EMBO J. 10:547-553(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
      Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
      Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. "Functionally important regions of glucose-6-phosphate dehydrogenase defined by the Saccharomyces cerevisiae enzyme and its differences from the mammalian and insect forms."
      Persson B., Joernvall H., Wood I., Jeffery J.
      Eur. J. Biochem. 198:485-491(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-505, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
    8. "Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae: characterization of a reactive lysine residue labeled with acetylsalicylic acid."
      Jeffery J., Hobbs L., Joernvall H.
      Biochemistry 24:666-671(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 185-195.
    9. "Fast atom bombardment mass spectrometry and chemical analysis in determinations of acyl-blocked protein structures."
      Egestad B., Estonius M., Danielsson O., Persson B., Cederlund E., Kaiser R., Holmquist B., Vallee B., Pares X., Jefferey J., Joernvall H.
      FEBS Lett. 269:194-196(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-7, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND TYR-145, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.

    Entry informationi

    Entry nameiG6PD_YEAST
    AccessioniPrimary (citable) accession number: P11412
    Secondary accession number(s): D6W0V2, E9P908
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 138 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 15000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3