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P11412

- G6PD_YEAST

UniProt

P11412 - G6PD_YEAST

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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

ZWF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521NADPBy similarity
Binding sitei157 – 1571NADP; via carbonyl oxygenBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Binding sitei225 – 2251SubstrateBy similarity
Binding sitei244 – 2441SubstrateBy similarity
Active sitei249 – 2491Proton acceptorBy similarity
Binding sitei343 – 3431SubstrateBy similarity
Binding sitei377 – 3771SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 258NADPBy similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: SGD
  2. NADP binding Source: InterPro

GO - Biological processi

  1. NADPH regeneration Source: SGD
  2. pentose-phosphate shunt, oxidative branch Source: SGD
  3. response to hydrogen peroxide Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:YNL241C-MONOMER.
YEAST:YNL241C-MONOMER.
SABIO-RKP11412.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:ZWF1
Synonyms:MET19
Ordered Locus Names:YNL241C
ORF Names:N1110
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

SGDiS000005185. ZWF1.

Pathology & Biotechi

Disruption phenotypei

Shows growth defects in acetate-supplemented medium; the phenotype is enhanced by addition of hydrogen peroxide (increased oxidative stress) and/or double knockout of ZWF1 and YHM2. The cytosolic NADPH/NADP+ ratio is decreased compared to wild type; this effect is enhanced in the presence of hydrogen peroxide. Double knockouts of ZWF1 and YHM2 show an increased mitochondrial NADPH/NADP+ ratio, but only in the presence of hydrogen peroxide. Levels of cytosolic citrate are reduced, but only in the presence of hydrogen peroxide. Levels of cytosolic oxoglutarate are reduced, but only in the presence of hydrogen peroxide. Cells show a moderate increase in levels of reactive oxygen species (ROS) following hydrogen peroxide treatment; in double knockouts of YHM2 and ZWF1 there is a larger increase in ROS.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 505504Glucose-6-phosphate 1-dehydrogenasePRO_0000068107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei142 – 1421Phosphoserine1 Publication
Modified residuei145 – 1451Phosphotyrosine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP11412.
PaxDbiP11412.
PeptideAtlasiP11412.
PRIDEiP11412.

2D gel databases

SWISS-2DPAGEP11412.

Expressioni

Gene expression databases

GenevestigatoriP11412.

Interactioni

Protein-protein interaction databases

BioGridi35598. 43 interactions.
DIPiDIP-5061N.
IntActiP11412. 4 interactions.
MINTiMINT-501936.
STRINGi4932.YNL241C.

Structurei

3D structure databases

ProteinModelPortaliP11412.
SMRiP11412. Positions 14-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni187 – 1915Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0364.
GeneTreeiENSGT00530000063435.
HOGENOMiHOG000046192.
InParanoidiP11412.
KOiK00036.
OMAiYCVTAVS.
OrthoDBiEOG7TXKRP.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11412-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEGPVKFEK NTVISVFGAS GDLAKKKTFP ALFGLFREGY LDPSTKIFGY
60 70 80 90 100
ARSKLSMEED LKSRVLPHLK KPHGEADDSK VEQFFKMVSY ISGNYDTDEG
110 120 130 140 150
FDELRTQIEK FEKSANVDVP HRLFYLALPP SVFLTVAKQI KSRVYAENGI
160 170 180 190 200
TRVIVEKPFG HDLASARELQ KNLGPLFKEE ELYRIDHYLG KELVKNLLVL
210 220 230 240 250
RFGNQFLNAS WNRDNIQSVQ ISFKERFGTE GRGGYFDSIG IIRDVMQNHL
260 270 280 290 300
LQIMTLLTME RPVSFDPESI RDEKVKVLKA VAPIDTDDVL LGQYGKSEDG
310 320 330 340 350
SKPAYVDDDT VDKDSKCVTF AAMTFNIENE RWEGVPIMMR AGKALNESKV
360 370 380 390 400
EIRLQYKAVA SGVFKDIPNN ELVIRVQPDA AVYLKFNAKT PGLSNATQVT
410 420 430 440 450
DLNLTYASRY QDFWIPEAYE VLIRDALLGD HSNFVRDDEL DISWGIFTPL
460 470 480 490 500
LKHIERPDGP TPEIYPYGSR GPKGLKEYMQ KHKYVMPEKH PYAWPVTKPE

DTKDN
Length:505
Mass (Da):57,522
Last modified:January 23, 2007 - v4
Checksum:i9FC23E6CE599454E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591Missing AA sequence (PubMed:2040308)Curated
Sequence conflicti80 – 801K → N in AAT93017. (PubMed:17322287)Curated
Sequence conflicti175 – 1751P → A in AAA34619. (PubMed:2269430)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34709 Genomic DNA. Translation: AAA34619.1.
X57336 Genomic DNA. Translation: CAA40611.1.
Z69381 Genomic DNA. Translation: CAA93357.1.
Z71517 Genomic DNA. Translation: CAA96146.1.
AY692998 Genomic DNA. Translation: AAT93017.1.
BK006947 Genomic DNA. Translation: DAA10318.1.
PIRiS13744.
RefSeqiNP_014158.1. NM_001183079.1.

Genome annotation databases

EnsemblFungiiYNL241C; YNL241C; YNL241C.
GeneIDi855480.
KEGGisce:YNL241C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M34709 Genomic DNA. Translation: AAA34619.1 .
X57336 Genomic DNA. Translation: CAA40611.1 .
Z69381 Genomic DNA. Translation: CAA93357.1 .
Z71517 Genomic DNA. Translation: CAA96146.1 .
AY692998 Genomic DNA. Translation: AAT93017.1 .
BK006947 Genomic DNA. Translation: DAA10318.1 .
PIRi S13744.
RefSeqi NP_014158.1. NM_001183079.1.

3D structure databases

ProteinModelPortali P11412.
SMRi P11412. Positions 14-484.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35598. 43 interactions.
DIPi DIP-5061N.
IntActi P11412. 4 interactions.
MINTi MINT-501936.
STRINGi 4932.YNL241C.

Chemistry

ChEMBLi CHEMBL1075249.

2D gel databases

SWISS-2DPAGE P11412.

Proteomic databases

MaxQBi P11412.
PaxDbi P11412.
PeptideAtlasi P11412.
PRIDEi P11412.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL241C ; YNL241C ; YNL241C .
GeneIDi 855480.
KEGGi sce:YNL241C.

Organism-specific databases

SGDi S000005185. ZWF1.

Phylogenomic databases

eggNOGi COG0364.
GeneTreei ENSGT00530000063435.
HOGENOMi HOG000046192.
InParanoidi P11412.
KOi K00036.
OMAi YCVTAVS.
OrthoDBi EOG7TXKRP.

Enzyme and pathway databases

UniPathwayi UPA00115 ; UER00408 .
BioCyci MetaCyc:YNL241C-MONOMER.
YEAST:YNL241C-MONOMER.
SABIO-RK P11412.

Miscellaneous databases

NextBioi 979445.
PROi P11412.

Gene expression databases

Genevestigatori P11412.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00966. G6PD.
InterProi IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23429. PTHR23429. 1 hit.
Pfami PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000110. G6PD. 1 hit.
PRINTSi PR00079. G6PDHDRGNASE.
TIGRFAMsi TIGR00871. zwf. 1 hit.
PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase."
    Nogae I., Johnston M.
    Gene 96:161-169(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur."
    Thomas D., Cherest H., Surdin-Kerjan Y.
    EMBO J. 10:547-553(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
    Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
    Yeast 12:1071-1076(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Functionally important regions of glucose-6-phosphate dehydrogenase defined by the Saccharomyces cerevisiae enzyme and its differences from the mammalian and insect forms."
    Persson B., Joernvall H., Wood I., Jeffery J.
    Eur. J. Biochem. 198:485-491(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-505, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
  8. "Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae: characterization of a reactive lysine residue labeled with acetylsalicylic acid."
    Jeffery J., Hobbs L., Joernvall H.
    Biochemistry 24:666-671(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 185-195.
  9. "Fast atom bombardment mass spectrometry and chemical analysis in determinations of acyl-blocked protein structures."
    Egestad B., Estonius M., Danielsson O., Persson B., Cederlund E., Kaiser R., Holmquist B., Vallee B., Pares X., Jefferey J., Joernvall H.
    FEBS Lett. 269:194-196(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-7, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142 AND TYR-145, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "Identification and functional characterization of a novel mitochondrial carrier for citrate and oxoglutarate in Saccharomyces cerevisiae."
    Castegna A., Scarcia P., Agrimi G., Palmieri L., Rottensteiner H., Spera I., Germinario L., Palmieri F.
    J. Biol. Chem. 285:17359-17370(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiG6PD_YEAST
AccessioniPrimary (citable) accession number: P11412
Secondary accession number(s): D6W0V2, E9P908
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3