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P11408

- MTM1_MORSP

UniProt

P11408 - MTM1_MORSP

Protein

Modification methylase MspI

Gene

mspIM

Organism
Moraxella sp.
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei174 – 1741PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3447. M.MspI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase MspI (EC:2.1.1.37)
    Short name:
    M.MspI
    Alternative name(s):
    Cytosine-specific methyltransferase MspI
    Gene namesi
    Name:mspIM
    OrganismiMoraxella sp.
    Taxonomic identifieri479 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 418418Modification methylase MspIPRO_0000087895Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP11408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini105 – 404300SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11408-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPEILKLIR SKLDLTQKQA SEIIEVSDKT WQQWESGKTE MHPAYYSFLQ    50
    EKLKDKINFE ELSAQKTLQK KIFDKYNQNQ ITKNAEELAE ITHIEERKDA 100
    YSSDFKFIDL FSGIGGIRQS FEVNGGKCVF SSEIDPFAKF TYYTNFGVVP 150
    FGDITKVEAT TIPQHDILCA GFPCQPFSHI GKREGFEHPT QGTMFHEIVR 200
    IIETKKTPVL FLENVPGLIN HDDGNTLKVI IETLEDMGYK VHHTVLDASH 250
    FGIPQKRKRF YLVAFLNQNI HFEFPKPPMI SKDIGEVLES DVTGYSISEH 300
    LQKSYLFKKD DGKPSLIDKN TTGAVKTLVS TYHKIQRLTG TFVKDGETGI 350
    RLLTTNECKA IMGFPKDFVI PVSRTQMYRQ MGNSVVVPVV TKIAEQISLA 400
    LKTVNQQSPQ ENFELELV 418
    Length:418
    Mass (Da):47,657
    Last modified:July 1, 1989 - v1
    Checksum:i7F8922F9CCAD926E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14191 Genomic DNA. Translation: CAA32393.1.
    PIRiS04188. CTKEMM.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14191 Genomic DNA. Translation: CAA32393.1 .
    PIRi S04188. CTKEMM.

    3D structure databases

    ProteinModelPortali P11408.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3447. M.MspI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the genes encoding the MspI restriction modification system."
      Lin P.M., Lee C.H., Roberts R.J.
      Nucleic Acids Res. 17:3001-3011(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49670.

    Entry informationi

    Entry nameiMTM1_MORSP
    AccessioniPrimary (citable) accession number: P11408
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3