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P11408

- MTM1_MORSP

UniProt

P11408 - MTM1_MORSP

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Protein
Modification methylase MspI
Gene
mspIM
Organism
Moraxella sp.
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei174 – 1741 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3447. M.MspI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MspI (EC:2.1.1.37)
Short name:
M.MspI
Alternative name(s):
Cytosine-specific methyltransferase MspI
Gene namesi
Name:mspIM
OrganismiMoraxella sp.
Taxonomic identifieri479 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Modification methylase MspI
PRO_0000087895Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP11408.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 404300SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11408-1 [UniParc]FASTAAdd to Basket

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MKPEILKLIR SKLDLTQKQA SEIIEVSDKT WQQWESGKTE MHPAYYSFLQ    50
EKLKDKINFE ELSAQKTLQK KIFDKYNQNQ ITKNAEELAE ITHIEERKDA 100
YSSDFKFIDL FSGIGGIRQS FEVNGGKCVF SSEIDPFAKF TYYTNFGVVP 150
FGDITKVEAT TIPQHDILCA GFPCQPFSHI GKREGFEHPT QGTMFHEIVR 200
IIETKKTPVL FLENVPGLIN HDDGNTLKVI IETLEDMGYK VHHTVLDASH 250
FGIPQKRKRF YLVAFLNQNI HFEFPKPPMI SKDIGEVLES DVTGYSISEH 300
LQKSYLFKKD DGKPSLIDKN TTGAVKTLVS TYHKIQRLTG TFVKDGETGI 350
RLLTTNECKA IMGFPKDFVI PVSRTQMYRQ MGNSVVVPVV TKIAEQISLA 400
LKTVNQQSPQ ENFELELV 418
Length:418
Mass (Da):47,657
Last modified:July 1, 1989 - v1
Checksum:i7F8922F9CCAD926E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14191 Genomic DNA. Translation: CAA32393.1.
PIRiS04188. CTKEMM.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14191 Genomic DNA. Translation: CAA32393.1 .
PIRi S04188. CTKEMM.

3D structure databases

ProteinModelPortali P11408.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3447. M.MspI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the genes encoding the MspI restriction modification system."
    Lin P.M., Lee C.H., Roberts R.J.
    Nucleic Acids Res. 17:3001-3011(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49670.

Entry informationi

Entry nameiMTM1_MORSP
AccessioniPrimary (citable) accession number: P11408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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