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P11408

- MTM1_MORSP

UniProt

P11408 - MTM1_MORSP

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Protein

Modification methylase MspI

Gene

mspIM

Organism
Moraxella sp.
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei174 – 1741PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3447. M.MspI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MspI (EC:2.1.1.37)
Short name:
M.MspI
Alternative name(s):
Cytosine-specific methyltransferase MspI
Gene namesi
Name:mspIM
OrganismiMoraxella sp.
Taxonomic identifieri479 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Modification methylase MspIPRO_0000087895Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP11408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 404300SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11408-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKPEILKLIR SKLDLTQKQA SEIIEVSDKT WQQWESGKTE MHPAYYSFLQ
60 70 80 90 100
EKLKDKINFE ELSAQKTLQK KIFDKYNQNQ ITKNAEELAE ITHIEERKDA
110 120 130 140 150
YSSDFKFIDL FSGIGGIRQS FEVNGGKCVF SSEIDPFAKF TYYTNFGVVP
160 170 180 190 200
FGDITKVEAT TIPQHDILCA GFPCQPFSHI GKREGFEHPT QGTMFHEIVR
210 220 230 240 250
IIETKKTPVL FLENVPGLIN HDDGNTLKVI IETLEDMGYK VHHTVLDASH
260 270 280 290 300
FGIPQKRKRF YLVAFLNQNI HFEFPKPPMI SKDIGEVLES DVTGYSISEH
310 320 330 340 350
LQKSYLFKKD DGKPSLIDKN TTGAVKTLVS TYHKIQRLTG TFVKDGETGI
360 370 380 390 400
RLLTTNECKA IMGFPKDFVI PVSRTQMYRQ MGNSVVVPVV TKIAEQISLA
410
LKTVNQQSPQ ENFELELV
Length:418
Mass (Da):47,657
Last modified:July 1, 1989 - v1
Checksum:i7F8922F9CCAD926E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14191 Genomic DNA. Translation: CAA32393.1.
PIRiS04188. CTKEMM.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14191 Genomic DNA. Translation: CAA32393.1 .
PIRi S04188. CTKEMM.

3D structure databases

ProteinModelPortali P11408.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3447. M.MspI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the genes encoding the MspI restriction modification system."
    Lin P.M., Lee C.H., Roberts R.J.
    Nucleic Acids Res. 17:3001-3011(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49670.

Entry informationi

Entry nameiMTM1_MORSP
AccessioniPrimary (citable) accession number: P11408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 1, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3