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P11408 (MTM1_MORSP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase MspI

Short name=M.MspI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase MspI
Gene names
Name:mspIM
OrganismMoraxella sp.
Taxonomic identifier479 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Modification methylase MspI
PRO_0000087895

Regions

Domain105 – 404300SAM-dependent MTase C5-type

Sites

Active site1741 By similarity

Sequences

Sequence LengthMass (Da)Tools
P11408 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 7F8922F9CCAD926E

FASTA41847,657
        10         20         30         40         50         60 
MKPEILKLIR SKLDLTQKQA SEIIEVSDKT WQQWESGKTE MHPAYYSFLQ EKLKDKINFE 

        70         80         90        100        110        120 
ELSAQKTLQK KIFDKYNQNQ ITKNAEELAE ITHIEERKDA YSSDFKFIDL FSGIGGIRQS 

       130        140        150        160        170        180 
FEVNGGKCVF SSEIDPFAKF TYYTNFGVVP FGDITKVEAT TIPQHDILCA GFPCQPFSHI 

       190        200        210        220        230        240 
GKREGFEHPT QGTMFHEIVR IIETKKTPVL FLENVPGLIN HDDGNTLKVI IETLEDMGYK 

       250        260        270        280        290        300 
VHHTVLDASH FGIPQKRKRF YLVAFLNQNI HFEFPKPPMI SKDIGEVLES DVTGYSISEH 

       310        320        330        340        350        360 
LQKSYLFKKD DGKPSLIDKN TTGAVKTLVS TYHKIQRLTG TFVKDGETGI RLLTTNECKA 

       370        380        390        400        410 
IMGFPKDFVI PVSRTQMYRQ MGNSVVVPVV TKIAEQISLA LKTVNQQSPQ ENFELELV 

« Hide

References

[1]"Cloning and characterization of the genes encoding the MspI restriction modification system."
Lin P.M., Lee C.H., Roberts R.J.
Nucleic Acids Res. 17:3001-3011(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49670.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14191 Genomic DNA. Translation: CAA32393.1.
PIRCTKEMM. S04188.

3D structure databases

ProteinModelPortalP11408.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3447. M.MspI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.260.40. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF47413. SSF47413. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTM1_MORSP
AccessionPrimary (citable) accession number: P11408
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries