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Protein

Modification methylase MspI

Gene

mspIM

Organism
Moraxella sp.
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei174PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MspI (EC:2.1.1.37)
Short name:
M.MspI
Alternative name(s):
Cytosine-specific methyltransferase MspI
Gene namesi
Name:mspIM
OrganismiMoraxella sp.
Taxonomic identifieri479 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878951 – 418Modification methylase MspIAdd BLAST418

Structurei

3D structure databases

ProteinModelPortaliP11408
SMRiP11408
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini105 – 404SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR001387 Cro/C1-type_HTH
IPR010982 Lambda_DNA-bd_dom_sf
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF47413 SSF47413, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P11408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPEILKLIR SKLDLTQKQA SEIIEVSDKT WQQWESGKTE MHPAYYSFLQ
60 70 80 90 100
EKLKDKINFE ELSAQKTLQK KIFDKYNQNQ ITKNAEELAE ITHIEERKDA
110 120 130 140 150
YSSDFKFIDL FSGIGGIRQS FEVNGGKCVF SSEIDPFAKF TYYTNFGVVP
160 170 180 190 200
FGDITKVEAT TIPQHDILCA GFPCQPFSHI GKREGFEHPT QGTMFHEIVR
210 220 230 240 250
IIETKKTPVL FLENVPGLIN HDDGNTLKVI IETLEDMGYK VHHTVLDASH
260 270 280 290 300
FGIPQKRKRF YLVAFLNQNI HFEFPKPPMI SKDIGEVLES DVTGYSISEH
310 320 330 340 350
LQKSYLFKKD DGKPSLIDKN TTGAVKTLVS TYHKIQRLTG TFVKDGETGI
360 370 380 390 400
RLLTTNECKA IMGFPKDFVI PVSRTQMYRQ MGNSVVVPVV TKIAEQISLA
410
LKTVNQQSPQ ENFELELV
Length:418
Mass (Da):47,657
Last modified:July 1, 1989 - v1
Checksum:i7F8922F9CCAD926E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14191 Genomic DNA Translation: CAA32393.1
PIRiS04188 CTKEMM

Similar proteinsi

Entry informationi

Entry nameiMTM1_MORSP
AccessioniPrimary (citable) accession number: P11408
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health