Reviewed, UniProtKB/Swiss-Prot P11408 (MTM1_MORSP)

Names and origin

Protein names

Recommended name:
    Modification methylase MspI
      Short name=M.MspI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase MspI

Gene names

Name:

mspIM

Organism

Moraxella sp.

Taxonomic identifier

479 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Moraxella

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Modification methylase MspI		PRO_0000087895
Sites
	Active site	174	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11408-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 7F8922F9CCAD926E	FASTA	418	47,657
	10 20 30 40 50 60 MKPEILKLIR SKLDLTQKQA SEIIEVSDKT WQQWESGKTE MHPAYYSFLQ EKLKDKINFE 70 80 90 100 110 120 ELSAQKTLQK KIFDKYNQNQ ITKNAEELAE ITHIEERKDA YSSDFKFIDL FSGIGGIRQS 130 140 150 160 170 180 FEVNGGKCVF SSEIDPFAKF TYYTNFGVVP FGDITKVEAT TIPQHDILCA GFPCQPFSHI 190 200 210 220 230 240 GKREGFEHPT QGTMFHEIVR IIETKKTPVL FLENVPGLIN HDDGNTLKVI IETLEDMGYK 250 260 270 280 290 300 VHHTVLDASH FGIPQKRKRF YLVAFLNQNI HFEFPKPPMI SKDIGEVLES DVTGYSISEH 310 320 330 340 350 360 LQKSYLFKKD DGKPSLIDKN TTGAVKTLVS TYHKIQRLTG TFVKDGETGI RLLTTNECKA 370 380 390 400 410 IMGFPKDFVI PVSRTQMYRQ MGNSVVVPVV TKIAEQISLA LKTVNQQSPQ ENFELELV

References

[1]	"Cloning and characterization of the genes encoding the MspI restriction modification system." Lin P.M., Lee C.H., Roberts R.J. Nucleic Acids Res. 17:3001-3011(1989) [PubMed: 2471145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49670.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14191 Genomic DNA. Translation: CAA32393.1.
PIR	CTKEMM. S04188.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3447. M.MspI.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR010982. Lambda_DNA-bd. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM1_MORSP

Accession

Primary (citable) accession number: P11408

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)