Reviewed, UniProtKB/Swiss-Prot P11405 (T2M1_MORSP)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme MspI
      Short name=R.MspI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme MspI
    Endonuclease MspI

Gene names

Name:

mspIR

Organism

Moraxella sp.

Taxonomic identifier

479 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Moraxella

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCGG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 262

262

Type-2 restriction enzyme MspI

PRO_0000077338

Secondary structure

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262

Helix Strand Turn

Details...

Helix

4 – 12

9

Helix

15 – 17

3

Helix

23 – 41

19

Helix

45 – 51

7

Helix

57 – 69

13

Helix

74 – 76

3

Beta strand

77 – 84

8

Beta strand

100 – 106

7

Beta strand

111 – 124

14

Beta strand

126 – 131

6

Helix

133 – 140

8

Helix

146 – 157

12

Helix

165 – 175

11

Helix

176 – 178

3

Helix

179 – 188

10

Beta strand

202 – 210

9

Beta strand

213 – 221

9

Helix

222 – 234

13

Helix

239 – 242

4

Beta strand

244 – 249

6

Beta strand

252 – 255

4

Sequences

	Sequence		Length	Mass (Da)
	P11405-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: E724275DCE50E6E8	FASTA	262	29,829
	10 20 30 40 50 60 MRTELLSKLY DDFGIDQLPH TQHGVTSDRL GKLYEKYILD IFKDIESLKK YNTNAFPQEK 70 80 90 100 110 120 DISSKLLKAL NLDLDNIIDV SSSDTDLGRT IAGGSPKTDA TIRFTFHNQS SRLVPLNIKH 130 140 150 160 170 180 SSKKKVSIAE YDVETICTGV GISDGELKEL IRKHQNDQSA KLFTPVQKQR LTELLEPYRE 190 200 210 220 230 240 RFIRWCVTLR AEKSEGNILH PDLLIRFQVI DREYVDVTIK NIDDYVSDRI AEGSKARKPG 250 260 FGTGLNWTYA SGSKAKKMQF KG

References

[1]	"Cloning and characterization of the genes encoding the MspI restriction modification system." Lin P.M., Lee C.H., Roberts R.J. Nucleic Acids Res. 17:3001-3011(1989) [PubMed: 2471145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49670.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X14191 Genomic DNA. Translation: CAA32394.1.

PIR

NDKE2M. S04187.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SA3	X-ray	1.95	A/B	1-262	[»]
1YFI	X-ray	2.70	A/B	1-262	[»]

ModBase

Search...

Protein family/group databases

REBASE

1277. MspI.

Family and domain databases

InterPro

IPR015291. Restrict_endonuc_II_MspI.
[Graphical view]

Pfam

PF09208. Endonuc-MspI. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

T2M1_MORSP

Accession

Primary (citable) accession number: P11405

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)