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Protein

Type-2 restriction enzyme MspI

Gene

mspIR

Organism
Moraxella sp.
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1277. MspI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MspI (EC:3.1.21.4)
Short name:
R.MspI
Alternative name(s):
Endonuclease MspI
Type II restriction enzyme MspI
Gene namesi
Name:mspIR
OrganismiMoraxella sp.
Taxonomic identifieri479 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773381 – 262Type-2 restriction enzyme MspIAdd BLAST262

Structurei

Secondary structure

1262
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 12Combined sources9
Helixi15 – 17Combined sources3
Helixi23 – 41Combined sources19
Helixi45 – 51Combined sources7
Helixi57 – 69Combined sources13
Helixi74 – 76Combined sources3
Beta strandi77 – 84Combined sources8
Beta strandi100 – 106Combined sources7
Beta strandi111 – 124Combined sources14
Beta strandi126 – 131Combined sources6
Helixi133 – 140Combined sources8
Helixi146 – 157Combined sources12
Helixi165 – 175Combined sources11
Helixi176 – 178Combined sources3
Helixi179 – 188Combined sources10
Beta strandi202 – 210Combined sources9
Beta strandi213 – 221Combined sources9
Helixi222 – 234Combined sources13
Helixi239 – 242Combined sources4
Beta strandi244 – 249Combined sources6
Beta strandi252 – 255Combined sources4
Beta strandi258 – 262Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SA3X-ray1.95A/B1-262[»]
1YFIX-ray2.70A/B1-262[»]
ProteinModelPortaliP11405.
SMRiP11405.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11405.

Family & Domainsi

Family and domain databases

InterProiIPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view]
PfamiPF09208. Endonuc-MspI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P11405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTELLSKLY DDFGIDQLPH TQHGVTSDRL GKLYEKYILD IFKDIESLKK
60 70 80 90 100
YNTNAFPQEK DISSKLLKAL NLDLDNIIDV SSSDTDLGRT IAGGSPKTDA
110 120 130 140 150
TIRFTFHNQS SRLVPLNIKH SSKKKVSIAE YDVETICTGV GISDGELKEL
160 170 180 190 200
IRKHQNDQSA KLFTPVQKQR LTELLEPYRE RFIRWCVTLR AEKSEGNILH
210 220 230 240 250
PDLLIRFQVI DREYVDVTIK NIDDYVSDRI AEGSKARKPG FGTGLNWTYA
260
SGSKAKKMQF KG
Length:262
Mass (Da):29,829
Last modified:July 1, 1989 - v1
Checksum:iE724275DCE50E6E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14191 Genomic DNA. Translation: CAA32394.1.
PIRiS04187. NDKE2M.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14191 Genomic DNA. Translation: CAA32394.1.
PIRiS04187. NDKE2M.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SA3X-ray1.95A/B1-262[»]
1YFIX-ray2.70A/B1-262[»]
ProteinModelPortaliP11405.
SMRiP11405.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1277. MspI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP11405.

Family and domain databases

InterProiIPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view]
PfamiPF09208. Endonuc-MspI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2M1_MORSP
AccessioniPrimary (citable) accession number: P11405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.