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P11405

- T2M1_MORSP

UniProt

P11405 - T2M1_MORSP

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Protein

Type-2 restriction enzyme MspI

Gene

mspIR

Organism
Moraxella sp.
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1277. MspI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MspI (EC:3.1.21.4)
Short name:
R.MspI
Alternative name(s):
Endonuclease MspI
Type II restriction enzyme MspI
Gene namesi
Name:mspIR
OrganismiMoraxella sp.
Taxonomic identifieri479 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Type-2 restriction enzyme MspIPRO_0000077338Add
BLAST

Structurei

Secondary structure

1
262
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 129Combined sources
Helixi15 – 173Combined sources
Helixi23 – 4119Combined sources
Helixi45 – 517Combined sources
Helixi57 – 6913Combined sources
Helixi74 – 763Combined sources
Beta strandi77 – 848Combined sources
Beta strandi100 – 1067Combined sources
Beta strandi111 – 12414Combined sources
Beta strandi126 – 1316Combined sources
Helixi133 – 1408Combined sources
Helixi146 – 15712Combined sources
Helixi165 – 17511Combined sources
Helixi176 – 1783Combined sources
Helixi179 – 18810Combined sources
Beta strandi202 – 2109Combined sources
Beta strandi213 – 2219Combined sources
Helixi222 – 23413Combined sources
Helixi239 – 2424Combined sources
Beta strandi244 – 2496Combined sources
Beta strandi252 – 2554Combined sources
Beta strandi258 – 2625Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SA3X-ray1.95A/B1-262[»]
1YFIX-ray2.70A/B1-262[»]
ProteinModelPortaliP11405.
SMRiP11405. Positions 1-262.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11405.

Family & Domainsi

Family and domain databases

InterProiIPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view]
PfamiPF09208. Endonuc-MspI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P11405-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTELLSKLY DDFGIDQLPH TQHGVTSDRL GKLYEKYILD IFKDIESLKK
60 70 80 90 100
YNTNAFPQEK DISSKLLKAL NLDLDNIIDV SSSDTDLGRT IAGGSPKTDA
110 120 130 140 150
TIRFTFHNQS SRLVPLNIKH SSKKKVSIAE YDVETICTGV GISDGELKEL
160 170 180 190 200
IRKHQNDQSA KLFTPVQKQR LTELLEPYRE RFIRWCVTLR AEKSEGNILH
210 220 230 240 250
PDLLIRFQVI DREYVDVTIK NIDDYVSDRI AEGSKARKPG FGTGLNWTYA
260
SGSKAKKMQF KG
Length:262
Mass (Da):29,829
Last modified:July 1, 1989 - v1
Checksum:iE724275DCE50E6E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14191 Genomic DNA. Translation: CAA32394.1.
PIRiS04187. NDKE2M.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14191 Genomic DNA. Translation: CAA32394.1 .
PIRi S04187. NDKE2M.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SA3 X-ray 1.95 A/B 1-262 [» ]
1YFI X-ray 2.70 A/B 1-262 [» ]
ProteinModelPortali P11405.
SMRi P11405. Positions 1-262.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1277. MspI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P11405.

Family and domain databases

InterProi IPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view ]
Pfami PF09208. Endonuc-MspI. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the genes encoding the MspI restriction modification system."
    Lin P.M., Lee C.H., Roberts R.J.
    Nucleic Acids Res. 17:3001-3011(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49670.

Entry informationi

Entry nameiT2M1_MORSP
AccessioniPrimary (citable) accession number: P11405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 26, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3