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P11405

- T2M1_MORSP

UniProt

P11405 - T2M1_MORSP

Protein

Type-2 restriction enzyme MspI

Gene

mspIR

Organism
Moraxella sp.
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (16 Apr 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CCGG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1277. MspI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MspI (EC:3.1.21.4)
    Short name:
    R.MspI
    Alternative name(s):
    Endonuclease MspI
    Type II restriction enzyme MspI
    Gene namesi
    Name:mspIR
    OrganismiMoraxella sp.
    Taxonomic identifieri479 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Type-2 restriction enzyme MspIPRO_0000077338Add
    BLAST

    Structurei

    Secondary structure

    1
    262
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 129
    Helixi15 – 173
    Helixi23 – 4119
    Helixi45 – 517
    Helixi57 – 6913
    Helixi74 – 763
    Beta strandi77 – 848
    Beta strandi100 – 1067
    Beta strandi111 – 12414
    Beta strandi126 – 1316
    Helixi133 – 1408
    Helixi146 – 15712
    Helixi165 – 17511
    Helixi176 – 1783
    Helixi179 – 18810
    Beta strandi202 – 2109
    Beta strandi213 – 2219
    Helixi222 – 23413
    Helixi239 – 2424
    Beta strandi244 – 2496
    Beta strandi252 – 2554
    Beta strandi258 – 2625

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SA3X-ray1.95A/B1-262[»]
    1YFIX-ray2.70A/B1-262[»]
    ProteinModelPortaliP11405.
    SMRiP11405. Positions 1-262.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11405.

    Family & Domainsi

    Family and domain databases

    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR015291. Restrct_endonuc_II_MspI.
    [Graphical view]
    PfamiPF09208. Endonuc-MspI. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P11405-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTELLSKLY DDFGIDQLPH TQHGVTSDRL GKLYEKYILD IFKDIESLKK    50
    YNTNAFPQEK DISSKLLKAL NLDLDNIIDV SSSDTDLGRT IAGGSPKTDA 100
    TIRFTFHNQS SRLVPLNIKH SSKKKVSIAE YDVETICTGV GISDGELKEL 150
    IRKHQNDQSA KLFTPVQKQR LTELLEPYRE RFIRWCVTLR AEKSEGNILH 200
    PDLLIRFQVI DREYVDVTIK NIDDYVSDRI AEGSKARKPG FGTGLNWTYA 250
    SGSKAKKMQF KG 262
    Length:262
    Mass (Da):29,829
    Last modified:July 1, 1989 - v1
    Checksum:iE724275DCE50E6E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14191 Genomic DNA. Translation: CAA32394.1.
    PIRiS04187. NDKE2M.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14191 Genomic DNA. Translation: CAA32394.1 .
    PIRi S04187. NDKE2M.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SA3 X-ray 1.95 A/B 1-262 [» ]
    1YFI X-ray 2.70 A/B 1-262 [» ]
    ProteinModelPortali P11405.
    SMRi P11405. Positions 1-262.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1277. MspI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P11405.

    Family and domain databases

    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR015291. Restrct_endonuc_II_MspI.
    [Graphical view ]
    Pfami PF09208. Endonuc-MspI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the genes encoding the MspI restriction modification system."
      Lin P.M., Lee C.H., Roberts R.J.
      Nucleic Acids Res. 17:3001-3011(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49670.

    Entry informationi

    Entry nameiT2M1_MORSP
    AccessioniPrimary (citable) accession number: P11405
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: April 16, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3