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P11405 (T2M1_MORSP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MspI

Short name=R.MspI
EC=3.1.21.4
Alternative name(s):
Endonuclease MspI
Type II restriction enzyme MspI
Gene names
Name:mspIR
OrganismMoraxella sp.
Taxonomic identifier479 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCGG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Type-2 restriction enzyme MspI
PRO_0000077338

Secondary structure

........................................ 262
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P11405 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: E724275DCE50E6E8

FASTA26229,829
        10         20         30         40         50         60 
MRTELLSKLY DDFGIDQLPH TQHGVTSDRL GKLYEKYILD IFKDIESLKK YNTNAFPQEK 

        70         80         90        100        110        120 
DISSKLLKAL NLDLDNIIDV SSSDTDLGRT IAGGSPKTDA TIRFTFHNQS SRLVPLNIKH 

       130        140        150        160        170        180 
SSKKKVSIAE YDVETICTGV GISDGELKEL IRKHQNDQSA KLFTPVQKQR LTELLEPYRE 

       190        200        210        220        230        240 
RFIRWCVTLR AEKSEGNILH PDLLIRFQVI DREYVDVTIK NIDDYVSDRI AEGSKARKPG 

       250        260 
FGTGLNWTYA SGSKAKKMQF KG 

« Hide

References

[1]"Cloning and characterization of the genes encoding the MspI restriction modification system."
Lin P.M., Lee C.H., Roberts R.J.
Nucleic Acids Res. 17:3001-3011(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49670.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14191 Genomic DNA. Translation: CAA32394.1.
PIRNDKE2M. S04187.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SA3X-ray1.95A/B1-262[»]
1YFIX-ray2.70A/B1-262[»]
ProteinModelPortalP11405.
SMRP11405. Positions 1-262.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1277. MspI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011335. Restrct_endonuc-II-like.
IPR015291. Restrct_endonuc_II_MspI.
[Graphical view]
PfamPF09208. Endonuc-MspI. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP11405.

Entry information

Entry nameT2M1_MORSP
AccessionPrimary (citable) accession number: P11405
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references