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P11386

- MDH_SULAC

UniProt

P11386 - MDH_SULAC

Protein

Malate dehydrogenase

Gene

mdh

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331NADBy similarity
    Binding sitei82 – 821SubstrateBy similarity
    Binding sitei88 – 881SubstrateBy similarity
    Binding sitei95 – 951NADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei148 – 1481SubstrateBy similarity
    Active sitei172 – 1721Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 136NADBy similarity
    Nucleotide bindingi118 – 1203NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSACI330779:GH9J-244-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:Saci_0246
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001018: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 306305Malate dehydrogenasePRO_0000113492Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi330779.Saci_0246.

    Structurei

    3D structure databases

    ProteinModelPortaliP11386.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213793.
    KOiK00024.
    OMAiHVVKQHL.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11386-1 [UniParc]FASTAAdd to Basket

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    MVKVAFIGVG RVGQTIAYNT IVNGYADEVM LYDVVPELPE KFEHEIRHAL    50
    AALRVKTELL STNNIDDISG ADIVVITAGK PRKPGMSRRD LFIDNAKIMI 100
    DLAKKLPKKN KGAMYIMVAN PVDMMASVFM KYSGENTIST GNQVETMRMR 150
    SYIAKKLNIP AYEVGGYVGG EHGEAAMVLW STVTVKGKPF SESLGVNKAE 200
    VEDYVKKIAA EIIRVLGGTT WGPGADIEEV IRSVALNEGK VMSVAFPHKY 250
    EDEIIHISEP VVVGRTVGPA LTSALDENDK ARLSQAIKEV YNVYKSNLKE 300
    LEQVIS 306
    Length:306
    Mass (Da):33,563
    Last modified:January 23, 2007 - v3
    Checksum:iE74C0CB278B3BA89
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111R → RG AA sequence (PubMed:2497031)Curated
    Sequence conflicti40 – 423EKF → TKK AA sequence (PubMed:2497031)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79663.1.
    PIRiS03958.
    RefSeqiYP_254956.1. NC_007181.1.

    Genome annotation databases

    EnsemblBacteriaiAAY79663; AAY79663; Saci_0246.
    GeneIDi3474728.
    KEGGisai:Saci_0246.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79663.1 .
    PIRi S03958.
    RefSeqi YP_254956.1. NC_007181.1.

    3D structure databases

    ProteinModelPortali P11386.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 330779.Saci_0246.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY79663 ; AAY79663 ; Saci_0246 .
    GeneIDi 3474728.
    KEGGi sai:Saci_0246.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213793.
    KOi K00024.
    OMAi HVVKQHL.

    Enzyme and pathway databases

    BioCyci SACI330779:GH9J-244-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
      Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
      J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
    2. "Archaebacterial malate dehydrogenase: the amino-terminal sequence of the enzyme from Sulfolobus acidocaldarius is homologous to the eubacterial and eukaryotic malate dehydrogenases."
      Gorisch H., Jany K.-D.
      FEBS Lett. 247:259-262(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-42.
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

    Entry informationi

    Entry nameiMDH_SULAC
    AccessioniPrimary (citable) accession number: P11386
    Secondary accession number(s): Q4JC16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3