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Protein

Malate dehydrogenase

Gene

mdh

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NADBy similarity1
Binding sitei82SubstrateBy similarity1
Binding sitei88SubstrateBy similarity1
Binding sitei95NADBy similarity1
Binding sitei120SubstrateBy similarity1
Binding sitei148SubstrateBy similarity1
Active sitei172Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13NADBy similarity6
Nucleotide bindingi118 – 120NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.37. 6160.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseBy similarity (EC:1.1.1.37By similarity)
Gene namesi
Name:mdh
Ordered Locus Names:Saci_0246
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001134922 – 306Malate dehydrogenaseAdd BLAST305

Interactioni

Protein-protein interaction databases

STRINGi330779.Saci_0246.

Structurei

3D structure databases

ProteinModelPortaliP11386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily.Curated

Phylogenomic databases

eggNOGiarCOG00246. Archaea.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00024.
OMAiTIAYSAM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVAFIGVG RVGQTIAYNT IVNGYADEVM LYDVVPELPE KFEHEIRHAL
60 70 80 90 100
AALRVKTELL STNNIDDISG ADIVVITAGK PRKPGMSRRD LFIDNAKIMI
110 120 130 140 150
DLAKKLPKKN KGAMYIMVAN PVDMMASVFM KYSGENTIST GNQVETMRMR
160 170 180 190 200
SYIAKKLNIP AYEVGGYVGG EHGEAAMVLW STVTVKGKPF SESLGVNKAE
210 220 230 240 250
VEDYVKKIAA EIIRVLGGTT WGPGADIEEV IRSVALNEGK VMSVAFPHKY
260 270 280 290 300
EDEIIHISEP VVVGRTVGPA LTSALDENDK ARLSQAIKEV YNVYKSNLKE

LEQVIS
Length:306
Mass (Da):33,563
Last modified:January 23, 2007 - v3
Checksum:iE74C0CB278B3BA89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11R → RG AA sequence (PubMed:2497031).Curated1
Sequence conflicti40 – 42EKF → TKK AA sequence (PubMed:2497031).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79663.1.
PIRiS03958.
RefSeqiWP_011277164.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79663; AAY79663; Saci_0246.
GeneIDi3474728.
KEGGisai:Saci_0246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79663.1.
PIRiS03958.
RefSeqiWP_011277164.1. NC_007181.1.

3D structure databases

ProteinModelPortaliP11386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_0246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY79663; AAY79663; Saci_0246.
GeneIDi3474728.
KEGGisai:Saci_0246.

Phylogenomic databases

eggNOGiarCOG00246. Archaea.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00024.
OMAiTIAYSAM.

Enzyme and pathway databases

BRENDAi1.1.1.37. 6160.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_SULAC
AccessioniPrimary (citable) accession number: P11386
Secondary accession number(s): Q4JC16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.