P11386 (MDH_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Saci_0246

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus acidocaldarius

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 306	305	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113492
Regions
	Nucleotide binding	8 – 13	6	NAD By similarity
	Nucleotide binding	118 – 120	3	NAD By similarity
Sites
	Active site	172	1	Proton acceptor By similarity
	Binding site	33	1	NAD By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	95	1	NAD By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
Experimental info
	Sequence conflict	11	1	R → RG AA sequence Ref.2
	Sequence conflict	40 – 42	3	EKF → TKK AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P11386 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E74C0CB278B3BA89	FASTA	306	33,563
	10 20 30 40 50 60 MVKVAFIGVG RVGQTIAYNT IVNGYADEVM LYDVVPELPE KFEHEIRHAL AALRVKTELL 70 80 90 100 110 120 STNNIDDISG ADIVVITAGK PRKPGMSRRD LFIDNAKIMI DLAKKLPKKN KGAMYIMVAN 130 140 150 160 170 180 PVDMMASVFM KYSGENTIST GNQVETMRMR SYIAKKLNIP AYEVGGYVGG EHGEAAMVLW 190 200 210 220 230 240 STVTVKGKPF SESLGVNKAE VEDYVKKIAA EIIRVLGGTT WGPGADIEEV IRSVALNEGK 250 260 270 280 290 300 VMSVAFPHKY EDEIIHISEP VVVGRTVGPA LTSALDENDK ARLSQAIKEV YNVYKSNLKE LEQVIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[2]	"Archaebacterial malate dehydrogenase: the amino-terminal sequence of the enzyme from Sulfolobus acidocaldarius is homologous to the eubacterial and eukaryotic malate dehydrogenases." Gorisch H., Jany K.-D. FEBS Lett. 247:259-262(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-42. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000077 Genomic DNA. Translation: AAY79663.1.
PIR	S03958.
RefSeq	YP_254956.1. NC_007181.1.
3D structure databases
ProteinModelPortal	P11386.
ModBase	Search...
Protein-protein interaction databases
STRING	330779.Saci_0246.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY79663; AAY79663; Saci_0246.
GeneID	3474728.
KEGG	sai:Saci_0246.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213793.
KO	K00024.
OMA	CLIDVCA.
ProtClustDB	CLSK883804.
Enzyme and pathway databases
BioCyc	SACI330779:GH9J-322-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3. Divergent sequence.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_SULAC

Accession

Primary (citable) accession number: P11386
Secondary accession number(s): Q4JC16

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program