P11371 (CP2C4_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C4
EC=1.14.14.1

Alternative name(s):
CYPIIC4
Cytochrome P450 PBc4
P1-88
Progesterone 21-hydroxylase

Gene names

Name:

CYP2C4

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane By similarity. Microsome membrane By similarity.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Miscellaneous	This protein differs from other forms of cytochrome P450 in that it catalyzes the 21-hydroxylation of progesterone, resulting in the formation of deoxycorticosterone.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Cytochrome P450 2C4		PRO_0000051695
Sites
	Metal binding	432	1	Iron (heme axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	P11371 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: F0E7F55F7512F36A	FASTA	487	55,387
	10 20 30 40 50 60 MDPVAGLVLG LCCLLLLSLW KQNSGRGKLP PGPTPFPIIG NILQIDVKDI SKSLTKFSER 70 80 90 100 110 120 YGPVFTVYLG MKPTVVLHGY KAVKEALVDL GEEFAGRGHF PIAEKVNKGL GIVFTNANTW 130 140 150 160 170 180 KEMRRFSLMT LRNFGMGKRS IEDRVQEEAR CLVEELRKTN ALPCDPTFIL GCAPCNVICS 190 200 210 220 230 240 VILHNRFDYK DEEFLKLMER LNENIRILSS PWLQVYNNFP ALLDYFPGIH KTLLKNADYT 250 260 270 280 290 300 KNFIMEKVKE HQKLLDVNNP RDFIDCFLIK MEKENNLEFT LGSLVIAVFD LFGAGTETTS 310 320 330 340 350 360 TTLRYSLLLL LKHPEVAARV QEEIERVIGR HRSPCMQDRS HMPYTDAVIH EIQRFIDLLP 370 380 390 400 410 420 TNLPHAVTRD VKFRNYFIPK GTDIITSLTS VLHDEKAFPN PKVFDPGHFL DESGNFKKSD 430 440 450 460 470 480 YFMPFSAGKR MCVGEGLARM ELFLFLTSIL QNFKLQSLVE PKDLDITAVV NGFVSVPPSY QLCFIPI

References

[1]	"Characterization of a second gene product related to rabbit cytochrome P-450 1." Johnson E.F., Barnes H.J., Griffin K.J., Okino S., Tukey R.H. J. Biol. Chem. 262:5918-5923(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Structure of 5' regions and expression of phenobarbital-inducible rabbit cytochrome P450IIC genes." Zhao J., Chan G., Govind S., Bell P., Kemper B.W. DNA Cell Biol. 9:37-48(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02716 mRNA. Translation: AAA31216.1. M74200 Genomic DNA. No translation available.
PIR	A26731.
RefSeq	NP_001177360.1. NM_001190431.1.
UniGene	Ocu.6430.
3D structure databases
ProteinModelPortal	P11371.
SMR	P11371. Positions 27-487.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100349436.
Organism-specific databases
CTD	100349436.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2C4_RABIT

Accession

Primary (citable) accession number: P11371

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program