Reviewed, UniProtKB/Swiss-Prot P11371 (CP2C4_RABIT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2C4
    EC=1.14.14.1
Alternative name(s):
    CYPIIC4
    Progesterone 21-hydroxylase
    P450 PBC4
    P1-88

Gene names

Name:

CYP2C4

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane By similarity. Microsome membrane By similarity.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Miscellaneous	This protein differs from other forms of cytochrome P450 in that it catalyzes the 21-hydroxylation of progesterone, resulting in the formation of deoxycorticosterone.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Cytochrome P450 2C4		PRO_0000051695
Sites
	Metal binding	432	1	Iron (heme axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	P11371-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: F0E7F55F7512F36A	FASTA	487	55,387
	10 20 30 40 50 60 MDPVAGLVLG LCCLLLLSLW KQNSGRGKLP PGPTPFPIIG NILQIDVKDI SKSLTKFSER 70 80 90 100 110 120 YGPVFTVYLG MKPTVVLHGY KAVKEALVDL GEEFAGRGHF PIAEKVNKGL GIVFTNANTW 130 140 150 160 170 180 KEMRRFSLMT LRNFGMGKRS IEDRVQEEAR CLVEELRKTN ALPCDPTFIL GCAPCNVICS 190 200 210 220 230 240 VILHNRFDYK DEEFLKLMER LNENIRILSS PWLQVYNNFP ALLDYFPGIH KTLLKNADYT 250 260 270 280 290 300 KNFIMEKVKE HQKLLDVNNP RDFIDCFLIK MEKENNLEFT LGSLVIAVFD LFGAGTETTS 310 320 330 340 350 360 TTLRYSLLLL LKHPEVAARV QEEIERVIGR HRSPCMQDRS HMPYTDAVIH EIQRFIDLLP 370 380 390 400 410 420 TNLPHAVTRD VKFRNYFIPK GTDIITSLTS VLHDEKAFPN PKVFDPGHFL DESGNFKKSD 430 440 450 460 470 480 YFMPFSAGKR MCVGEGLARM ELFLFLTSIL QNFKLQSLVE PKDLDITAVV NGFVSVPPSY QLCFIPI

References

[1]	"Characterization of a second gene product related to rabbit cytochrome P-450 1." Johnson E.F., Barnes H.J., Griffin K.J., Okino S., Tukey R.H. J. Biol. Chem. 262:5918-5923(1987) [PubMed: 3032948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Structure of 5' regions and expression of phenobarbital-inducible rabbit cytochrome P450IIC genes." Zhao J., Chan G., Govind S., Bell P., Kemper B.W. DNA Cell Biol. 9:37-48(1990) [PubMed: 2317269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02716 mRNA. Translation: AAA31216.1. M74200 Genomic DNA. No translation available.
PIR	A26731.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	P11371. Positions 44-487.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P11371.
Enzyme and pathway databases
BRENDA	1.14.14.1. 255.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2C4_RABIT

Accession

Primary (citable) accession number: P11371

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)