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Protein

LINE-1 retrotransposable element ORF2 protein

Gene

Pol

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has a reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Has also an endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to sites of LINE-1 integration in the genome.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi607 – 6071Magnesium; catalyticPROSITE-ProRule annotation
Metal bindingi709 – 7091Magnesium; catalyticPROSITE-ProRule annotation
Metal bindingi710 – 7101Magnesium; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA recombination

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
LINE-1 retrotransposable element ORF2 protein
Short name:
ORF2p
Alternative name(s):
Long interspersed element-1
Short name:
L1
Retrovirus-related Pol polyprotein LINE-1
Including the following 2 domains:
Reverse transcriptase (EC:2.7.7.49)
Endonuclease (EC:3.1.21.-)
Gene namesi
Name:Pol
Synonyms:Gm17492
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:4937126. Gm17492.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12811281LINE-1 retrotransposable element ORF2 proteinPRO_0000058509Add
BLAST

Proteomic databases

PaxDbiP11369.
PRIDEiP11369.

PTM databases

SwissPalmiP11369.

Structurei

3D structure databases

ProteinModelPortaliP11369.
SMRiP11369. Positions 10-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini505 – 780276Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini1247 – 126620DUF1725Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 245245Endonuclease activityBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 DUF1725 domain.Curated
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410J1P7. Eukaryota.
ENOG41120A8. LUCA.
HOVERGENiHBG006270.
InParanoidiP11369.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR013544. DUF1725.
IPR005135. Endo/exonuclease/phosphatase.
IPR000477. RT_dom.
[Graphical view]
PfamiPF08333. DUF1725. 1 hit.
PF03372. Exo_endo_phos. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTLTTKIKG SNNYFSLISL NINGLNSPIK RHRLTDWLHK QDPTFCCLQE
60 70 80 90 100
THLREKDRHY LRVKGWKTIF QANGLKKQAG VAILISDKID FQPKVIKKDK
110 120 130 140 150
EGHFILIKGK ILQEELSILN IYAPNARAAT FIRDTLVKLK AYIAPHTIIV
160 170 180 190 200
GDFNTPLSSK DRSWKQKLNR DTVKLTEVMK QMDLTDIYRT FYPKTKGYTF
210 220 230 240 250
FSAPHGTFSK IDHIIGHKTG LNRYKNIEIV PCILSDHHGL RLIFNNNINN
260 270 280 290 300
GKPTFTWKLN NTLLNDTLVK EGIKKEIKDF LEFNENEATT YPNLWDTMKA
310 320 330 340 350
FLRGKLIALS ASKKKRETAH TSSLTTHLKA LEKKEANSPK RSRRQEIIKL
360 370 380 390 400
RGEINQVETR RTIQRINQTR SWFFEKINKI DKPLARLTKG HRDKILINKI
410 420 430 440 450
RNEKGDITTD PEEIQNTIRS FYKRLYSTKL ENLDEMDKFL DRYQVPKLNQ
460 470 480 490 500
DQVDHLNSPI SPKEIEAVIN SLPTKKSPGP DGFSAEFYQT FKEDLIPILH
510 520 530 540 550
KLFHKIEVEG TLPNSFYEAT ITLIPKPQKD PTKIENFRPI SLMNIDAKIL
560 570 580 590 600
NKILANRIQE HIKAIIHPDQ VGFIPGMQGW FNIRKSINVI HYINKLKDKN
610 620 630 640 650
HMIISLDAEK AFDKIQHPFM IKVLERSGIQ GPYLNMIKAI YSKPVANIKV
660 670 680 690 700
NGEKLEAIPL KSGTRQGCPL SPYLFNIVLE VLARAIRQQK EIKGIQIGKE
710 720 730 740 750
EVKISLLADD MIVYISDPKN STRELLNLIN SFGEVVGYKI NSNKSMAFLY
760 770 780 790 800
TKNKQAEKEI RETTPFSIVT NNIKYLGVTL TKEVKDLYDK NFKSLKKEIK
810 820 830 840 850
EDLRRWKDLP CSWIGRINIV KMAILPKAIY RFNAIPIKIP TQFFNELEGA
860 870 880 890 900
ICKFVWNNKK PRIAKSLLKD KRTSGGITMP DLKLYYRAIV IKTAWYWYRD
910 920 930 940 950
RQVDQWNRIE DPEMNPHTYG HLIFDKGAKT IQWKKDSIFN NWCWHNWLLS
960 970 980 990 1000
CRRMRIDPYL SPCTKVKSKW IKELHIKPET LKLIEEKVGK SLEDMGTGEK
1010 1020 1030 1040 1050
FLNRTAMACA VRSRIDKWDL MKLQSFCKAK DTVNKTKRPP TDWERIFTYP
1060 1070 1080 1090 1100
KSDRGLISNI YKELKKVDFR KSNNPIKKWG SELNKEFSPE EYRMAEKHLK
1110 1120 1130 1140 1150
KCSTSLIIRE MQIKTTLRFH LTPVRMAKIK NSGDSRCWRG CGERGTLLHC
1160 1170 1180 1190 1200
WWECRLVQPL WKSVWRFLRK LDIVLPEDPA IPLLGIYPED APTGKKDTCS
1210 1220 1230 1240 1250
TMFIAALFII ARSWKEPRCP STEEWIQKMW YIYTMEYYSA IKKNEFMKFL
1260 1270 1280
AKWMDLEGII LSEVTHSQRN SHNMYSLISG Y
Length:1,281
Mass (Da):149,581
Last modified:February 1, 2005 - v2
Checksum:iA6D2894DA364AB19
GO

Sequence cautioni

The sequence CAA27363.1 differs from that shown. Reason: Frameshift at position 424. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861S → L in AAA67727 (PubMed:7533116).Curated
Sequence conflicti246 – 2461N → K in CAA27363 (PubMed:3008107).Curated
Sequence conflicti359 – 3591T → K in AAA67727 (PubMed:7533116).Curated
Sequence conflicti707 – 7071L → F in AAA67727 (PubMed:7533116).Curated
Sequence conflicti736 – 7361V → A in AAA67727 (PubMed:7533116).Curated
Sequence conflicti761 – 7611R → W in AAA67727 (PubMed:7533116).Curated
Sequence conflicti928 – 9281A → D in AAA67727 (PubMed:7533116).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13002 Genomic DNA. Translation: AAA66024.1.
U15647 mRNA. Translation: AAA67727.1.
X03725 Genomic DNA. Translation: CAA27363.1. Frameshift.
PIRiB58927. GNMSLL.

Genome annotation databases

UCSCiuc029qyg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13002 Genomic DNA. Translation: AAA66024.1.
U15647 mRNA. Translation: AAA67727.1.
X03725 Genomic DNA. Translation: CAA27363.1. Frameshift.
PIRiB58927. GNMSLL.

3D structure databases

ProteinModelPortaliP11369.
SMRiP11369. Positions 10-242.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

SwissPalmiP11369.

Proteomic databases

PaxDbiP11369.
PRIDEiP11369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc029qyg.1. mouse.

Organism-specific databases

MGIiMGI:4937126. Gm17492.

Phylogenomic databases

eggNOGiENOG410J1P7. Eukaryota.
ENOG41120A8. LUCA.
HOVERGENiHBG006270.
InParanoidiP11369.

Miscellaneous databases

NextBioi35563527.
PROiP11369.
SOURCEiSearch...

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR013544. DUF1725.
IPR005135. Endo/exonuclease/phosphatase.
IPR000477. RT_dom.
[Graphical view]
PfamiPF08333. DUF1725. 1 hit.
PF03372. Exo_endo_phos. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The sequence of a large L1Md element reveals a tandemly repeated 5' end and several features found in retrotransposons."
    Loeb D.D., Padgett R.W., Hardies S.C., Shehee W.R., Comer M.B., Edgell M.H., Hutchison C.A. III
    Mol. Cell. Biol. 6:168-182(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of a LINE-1 cDNA that originated from RNA present in ribonucleoprotein particles: implications for the structure of an active mouse LINE-1."
    Martin S.L.
    Gene 153:261-266(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Conservation in the 5' region of the long interspersed mouse L1 repeat: implications of comparative sequence analysis."
    Mottez E., Rogan P.K., Manuelidis L.
    Nucleic Acids Res. 14:3119-3136(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-484.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 99-108, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiLORF2_MOUSE
AccessioniPrimary (citable) accession number: P11369
Secondary accession number(s): Q60713, Q61787
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 2005
Last modified: May 11, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

An active LINE-1 encodes for 2 proteins translated from a single RNA containing two non-overlapping ORFs, ORF1 and ORF2. ORF2p is described in that entry as a representative of all ORF2p potentially expressed by active elements in mouse genome. ORF1p is described in the related entry AC P11260.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.