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Protein

Intracisternal A-particle Gag-related polyprotein

Gene

gag

Organism
Mouse intracisternal a-particle MIA14 (IAP-MIA14)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Protease: The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.PROSITE-ProRule annotation

Miscellaneous

Intracisternal A-particle Gag-related polyprotein: Intracisternal A particles (IAPs) are defective retroviral elements. Due to extensive mutations in the envelope coding sequence, IAPs can only form defective viral particles confined to the intracisternae of the Golgi. IAPs are an important class of transposable elements that induce genomic mutations and cell transformation by disrupting gene expression.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei695Protease; shared with dimeric partnerPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri458 – 475CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri484 – 501CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiA02.016

Names & Taxonomyi

Protein namesi
Recommended name:
Intracisternal A-particle Gag-related polyprotein
Cleaved into the following 4 chains:
Protease (EC:3.4.23.-PROSITE-ProRule annotation)
Gene namesi
Name:gag
OrganismiMouse intracisternal a-particle MIA14 (IAP-MIA14)
Taxonomic identifieri11753 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeIntracisternal A-particles
Virus hostiMus musculus (Mouse) [TaxID: 10090]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000026112? – 827Protease
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000044328026 – 827Intracisternal A-particle Gag-related polyproteinAdd BLAST802
ChainiPRO_000044328126 – 217Phosphorylated proteinBy similarityAdd BLAST192
ChainiPRO_0000443282218 – 441Capsid proteinBy similarityAdd BLAST224
ChainiPRO_0000443283442 – ?Nucleocapsid protein

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei22 – 23Cleavage; by viral proteaseBy similarity2
Sitei217 – 218Cleavage; by viral proteaseBy similarity2
Sitei261 – 262Cleavage; by viral proteaseBy similarity2
Sitei441 – 442Cleavage; by viral proteaseBy similarity2

Proteomic databases

PRIDEiP11365

Structurei

3D structure databases

ProteinModelPortaliP11365
SMRiP11365
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini690 – 765Peptidase A2PROSITE-ProRule annotationAdd BLAST76
Domaini783 – 827G-patchPROSITE-ProRule annotationAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi641 – 645Poly-LeuSequence analysis5

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri458 – 475CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri484 – 501CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Signal, Zinc-finger

Family and domain databases

CDDicd05482 HIV_retropepsin_like, 1 hit
Gene3Di1.10.1200.30, 1 hit
1.10.375.10, 1 hit
2.40.70.10, 1 hit
2.70.40.10, 1 hit
InterProiView protein in InterPro
IPR001969 Aspartic_peptidase_AS
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR000467 G_patch_dom
IPR000721 Gag_p24
IPR001995 Peptidase_A2_cat
IPR021109 Peptidase_aspartic_dom_sf
IPR034170 Retropepsin-like_cat_dom
IPR018061 Retropepsins
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
PF01585 G-patch, 1 hit
PF00607 Gag_p24, 1 hit
PF00077 RVP, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00443 G_patch, 1 hit
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47943 SSF47943, 1 hit
SSF50630 SSF50630, 1 hit
SSF51283 SSF51283, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50175 ASP_PROT_RETROV, 1 hit
PS00141 ASP_PROTEASE, 1 hit
PS50174 G_PATCH, 1 hit
PS50158 ZF_CCHC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11365-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGLEFFLVL EALLFLFTCY QVVKAGRILD EIQDKLSEVK RGERVGTKRK
60 70 80 90 100
YGTQNKYTGL SKGLEPEEKL RLGRNTWREI RRKRGKREKK KDQLAEVSRK
110 120 130 140 150
RSLCSSLDGL GEPALSSSEA DEEFSSEETD WEEEAAHYEK KGYQPGKVLA
160 170 180 190 200
NQLRKPKAAG EGQFADWPQG SRLQGPPYAE SPPCVVRQPC AERQCAKRQC
210 220 230 240 250
ADSFIPREEQ RKIQQAFPVF EGAEGGRVHA PVEYLQIKEL AESVRKYGTN
260 270 280 290 300
ANFTLVQLDR LAGMALTPAD WQTVVKAALP MMGKYMEWRA LWHETAQAQA
310 320 330 340 350
RANAAALTPE QRDWTFDLLT GQGAYSADQT NYHWGAYAQI SSTAIRRWKG
360 370 380 390 400
LSRAGETTGQ LTKVVQGPQE SFSDFVARMT EAAERIFGES EQAAPLIEQL
410 420 430 440 450
IYEQATKECR AAIAPRKNKG LQDWLRVCRE LGGPLTNAGL AAAILQSQNR
460 470 480 490 500
SMSRNDQRTC FNCGKPGHFK KDCRAPDKQG GTLTLCSKCG KGYHRADQCR
510 520 530 540 550
SVRDIKGRVL PPPDSQSAYV PKNGSSGPRS QGLKDMGTGL SGPRKQSERR
560 570 580 590 600
PRKTHKVDLR AASDFLLMPQ MSIQPVPVEP IPSLPLGTMG LILGRGSAST
610 620 630 640 650
LQGLVVHPEL WIVNIPQKYQ VLCSSPKGVF SISKGDRIPQ LLLLLPDNTR
660 670 680 690 700
EKSAGPEIKK MGSSGNDSAY LVVSLNDRPK LRLKINGKEF EGILDTGADK
710 720 730 740 750
SIISTHWWPK AWPTTESSHS LQGLGYQSCP TISSVALTWE SSEGQQGKFI
760 770 780 790 800
PYVLPLPVNL WGRDIMQHLG LILSNENAPS GGYSAKAKNI MAKMGYKEGK
810 820
GLGHQEQGRI EPISPNGNQD RQGLGFP
Length:827
Mass (Da):91,593
Last modified:March 27, 2002 - v2
Checksum:i697EC68C314375DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17551 Genomic DNA Translation: AAC12789.1
PIRiA26787 FOMSIA

Similar proteinsi

Entry informationi

Entry nameiGAG_IPMA
AccessioniPrimary (citable) accession number: P11365
Secondary accession number(s): Q61577
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: March 27, 2002
Last modified: May 23, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Transposable element

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