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Protein

Glutathione peroxidase 1

Gene

Gpx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protects the hemoglobin in erythrocytes from oxidative breakdown.

Miscellaneous

In the absence of Sod1, Gpx1 in the liver undergoes a 40% reduction in catalytic activity as a result of the decomposition of Sec-47 to dehydroalanine.1 Publication

Catalytic activityi

2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Kineticsi

  1. KM=14 µM for H2O2 (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication
  2. KM=29 µM for tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication
  1. Vmax=319 mM/min/mg enzyme toward H2O2 (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication
  2. Vmax=182 mM/min/mg enzyme toward tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei471
Sitei47Subject to oxidation and hydroselenide loss to dehydroalanine1

GO - Molecular functioni

  • glutathione peroxidase activity Source: MGI
  • SH3 domain binding Source: MGI

GO - Biological processi

  • angiogenesis involved in wound healing Source: MGI
  • apoptotic process Source: MGI
  • blood vessel endothelial cell migration Source: MGI
  • cell proliferation Source: MGI
  • cell redox homeostasis Source: MGI
  • endothelial cell development Source: MGI
  • fat cell differentiation Source: MGI
  • glutathione metabolic process Source: MGI
  • heart contraction Source: MGI
  • hydrogen peroxide catabolic process Source: MGI
  • interaction with symbiont Source: MGI
  • intrinsic apoptotic signaling pathway in response to oxidative stress Source: MGI
  • lipid metabolic process Source: MGI
  • myoblast proliferation Source: MGI
  • myotube differentiation Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: MGI
  • negative regulation of inflammatory response to antigenic stimulus Source: MGI
  • negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: MGI
  • negative regulation of release of cytochrome c from mitochondria Source: MGI
  • positive regulation of protein kinase B signaling Source: MGI
  • protein oxidation Source: MGI
  • regulation of gene expression, epigenetic Source: MGI
  • regulation of mammary gland epithelial cell proliferation Source: MGI
  • regulation of neuron apoptotic process Source: MGI
  • regulation of proteasomal protein catabolic process Source: MGI
  • response to gamma radiation Source: MGI
  • response to hydrogen peroxide Source: MGI
  • response to hydroperoxide Source: MGI
  • response to oxidative stress Source: MGI
  • response to reactive oxygen species Source: MGI
  • response to selenium ion Source: MGI
  • response to symbiotic bacterium Source: MGI
  • response to toxic substance Source: MGI
  • response to wounding Source: MGI
  • response to xenobiotic stimulus Source: MGI
  • sensory perception of sound Source: MGI
  • skeletal muscle fiber development Source: MGI
  • skeletal muscle tissue regeneration Source: MGI
  • temperature homeostasis Source: MGI
  • triglyceride metabolic process Source: MGI
  • UV protection Source: MGI
  • vasodilation Source: MGI

Keywordsi

Molecular functionOxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-MMU-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-MMU-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
R-MMU-3299685. Detoxification of Reactive Oxygen Species.
R-MMU-74259. Purine catabolism.
SABIO-RKiP11352.

Protein family/group databases

PeroxiBasei3709. MmGPx01.

Chemistry databases

SwissLipidsiSLP:000001635.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione peroxidase 1 (EC:1.11.1.9)
Short name:
GPx-1
Short name:
GSHPx-1
Alternative name(s):
Cellular glutathione peroxidase
Selenium-dependent glutathione peroxidase 1
Gene namesi
Name:Gpx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104887. Gpx1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666131 – 201Glutathione peroxidase 1Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineCombined sources1
Modified residuei32PhosphoserineBy similarity1
Modified residuei62N6-acetyllysine; alternateCombined sources1
Modified residuei62N6-succinyllysine; alternateCombined sources1
Modified residuei86N6-acetyllysine; alternateCombined sources1
Modified residuei86N6-succinyllysine; alternateCombined sources1
Modified residuei112N6-acetyllysine; alternateCombined sources1
Modified residuei112N6-succinyllysine; alternateCombined sources1
Modified residuei119N6-acetyllysineCombined sources1
Modified residuei146N6-acetyllysine; alternateCombined sources1
Modified residuei146N6-succinyllysine; alternateCombined sources1
Modified residuei195PhosphoserineBy similarity1

Post-translational modificationi

During periods of oxidative stress, Sec-47 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP11352.
MaxQBiP11352.
PaxDbiP11352.
PeptideAtlasiP11352.
PRIDEiP11352.

2D gel databases

REPRODUCTION-2DPAGEiIPI00319652.
P11352.
SWISS-2DPAGEiP11352.

PTM databases

iPTMnetiP11352.
PhosphoSitePlusiP11352.
SwissPalmiP11352.

Expressioni

Gene expression databases

BgeeiENSMUSG00000063856.
CleanExiMM_GPX1.
ExpressionAtlasiP11352. baseline and differential.
GenevisibleiP11352. MM.

Interactioni

Subunit structurei

Homotetramer. Interacts with MIEN1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200037. 2 interactors.
IntActiP11352. 8 interactors.
MINTiMINT-1855025.
STRINGi10090.ENSMUSP00000081010.

Structurei

3D structure databases

ProteinModelPortaliP11352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
GeneTreeiENSGT00760000119230.
HOGENOMiHOG000277055.
HOVERGENiHBG004333.
InParanoidiP11352.
KOiK00432.
OMAiVDRYYPT.
OrthoDBiEOG091G10LN.
PhylomeDBiP11352.
TreeFamiTF105318.

Family and domain databases

CDDicd00340. GSH_Peroxidase. 1 hit.
InterProiView protein in InterPro
IPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR036249. Thioredoxin-like_sf.
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiView protein in Pfam
PF00255. GSHPx. 1 hit.
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P11352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAARLSAAA QSTVYAFSAR PLTGGEPVSL GSLRGKVLLI ENVASLUGTT
60 70 80 90 100
IRDYTEMNDL QKRLGPRGLV VLGFPCNQFG HQENGKNEEI LNSLKYVRPG
110 120 130 140 150
GGFEPNFTLF EKCEVNGEKA HPLFTFLRNA LPTPSDDPTA LMTDPKYIIW
160 170 180 190 200
SPVCRNDIAW NFEKFLVGPD GVPVRRYSRR FRTIDIEPDI ETLLSQQSGN

S
Length:201
Mass (Da):22,329
Last modified:February 26, 2008 - v2
Checksum:i401D065165D8AF5C
GO

Sequence cautioni

P11352: The sequence CAB43535 differs from that shown. Number of sequencing artifacts.Curated

Non-standard residue

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-standard residuei47Selenocysteine1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03920 Genomic DNA. Translation: CAA27558.1.
AK002245 mRNA. Translation: BAC55244.1.
AK010999 mRNA. Translation: BAC55252.1.
AK011019 mRNA. Translation: BAC55253.1.
AK028171 mRNA. Translation: BAC55257.1.
AK150548 mRNA. Translation: BAE29650.1.
AK154833 mRNA. Translation: BAE32862.1.
AK160388 mRNA. Translation: BAE35760.1.
BC086649 mRNA. Translation: AAH86649.1.
X15667 mRNA. Translation: CAB43535.1. Sequence problems.
CCDSiCCDS23522.1.
PIRiA25106. OPMSE.
S05317.
RefSeqiNP_001316456.1. NM_001329527.1.
NP_001316457.1. NM_001329528.1.
NP_032186.2. NM_008160.6.
UniGeneiMm.1090.

Genome annotation databases

EnsembliENSMUST00000082429; ENSMUSP00000081010; ENSMUSG00000063856.
GeneIDi14775.
KEGGimmu:14775.
UCSCiuc009rpf.3. mouse.

Keywords - Coding sequence diversityi

Selenocysteine

Similar proteinsi

Entry informationi

Entry nameiGPX1_MOUSE
AccessioniPrimary (citable) accession number: P11352
Secondary accession number(s): P12079
, Q544W3, Q5RJH8, Q9CR54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 26, 2008
Last modified: October 25, 2017
This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:2771650 sequence was originally thought to originate from human.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families