P11352 (GPX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione peroxidase 1
Short name=GPx-1
Short name=GSHPx-1
EC=1.11.1.9

Alternative name(s):
Cellular glutathione peroxidase
Selenium-dependent glutathione peroxidase 1

Gene names

Name:

Gpx1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protects the hemoglobin in erythrocytes from oxidative breakdown.
Catalytic activity	2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
Subunit structure	Homotetramer. Interacts with MIEN1. Ref.6
Subcellular location	Cytoplasm.
Post-translational modification	During periods of oxidative stress, Sec-47 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine (Ref.8).
Miscellaneous	In the absence of Sod1, Gpx1 in the liver undergoes a 40% reduction in catalytic activity as a result of the decomposition of Sec-47 to dehydroalanine (Ref.8).
Sequence similarities	Belongs to the glutathione peroxidase family.
Caution	Ref.4 sequence was originally thought to originate from human.
Biophysicochemical properties	Kinetic parameters: KM=14 µM for H2O2 (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0) Ref.8 KM=29 µM for tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0) Vmax=319 mM/min/mg enzyme toward H2O2 (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0) Vmax=182 mM/min/mg enzyme toward tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0)
Sequence caution	The sequence CAB43535.1 differs from that shown. Reason: Number of sequencing artifacts.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Selenocysteine
Molecular function	Oxidoreductase Peroxidase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UV protection Inferred from electronic annotation. Source: Compara angiogenesis involved in wound healing Inferred from mutant phenotype PubMed 16373599. Source: MGI blood vessel endothelial cell migration Inferred from mutant phenotype PubMed 16373599. Source: MGI cell redox homeostasis Inferred from electronic annotation. Source: Compara endothelial cell development Inferred from mutant phenotype PubMed 16373599. Source: MGI fat cell differentiation Inferred from mutant phenotype PubMed 16962942. Source: MGI glutathione metabolic process Inferred from electronic annotation. Source: Compara heart contraction Inferred from mutant phenotype PubMed 12196344. Source: MGI hydrogen peroxide catabolic process Inferred from mutant phenotype PubMed 10754271PubMed 10915565PubMed 11545230PubMed 15182862PubMed 15827346PubMed 9788901. Source: MGI induction of apoptosis Inferred from mutant phenotype PubMed 16373599. Source: MGI interaction with symbiont Inferred from genetic interaction PubMed 12751789. Source: MGI intrinsic apoptotic signaling pathway in response to oxidative stress Inferred from mutant phenotype PubMed 11562367PubMed 15663476. Source: MGI myoblast proliferation Inferred from mutant phenotype PubMed 12368211PubMed 16962942. Source: MGI myotube differentiation Inferred from mutant phenotype PubMed 12368211PubMed 16962942. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype PubMed 16934683. Source: MGI negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of inflammatory response to antigenic stimulus Inferred from genetic interaction PubMed 12751789. Source: MGI positive regulation of protein kinase B signaling cascade Inferred from mutant phenotype PubMed 15663476. Source: MGI protein oxidation Inferred from mutant phenotype PubMed 10428770. Source: MGI regulation of gene expression, epigenetic Inferred from electronic annotation. Source: Compara regulation of mammary gland epithelial cell proliferation Inferred from electronic annotation. Source: Compara regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 11579147. Source: MGI regulation of proteasomal protein catabolic process Inferred from electronic annotation. Source: Compara release of cytochrome c from mitochondria Inferred from electronic annotation. Source: Compara response to gamma radiation Inferred from genetic interaction PubMed 15182862. Source: MGI response to hydroperoxide Inferred from mutant phenotype PubMed 16723478. Source: MGI response to selenium ion Inferred from electronic annotation. Source: Compara response to symbiotic bacterium Inferred from genetic interaction PubMed 12751789. Source: MGI response to toxic substance Inferred from mutant phenotype PubMed 10627575. Source: MGI response to xenobiotic stimulus Inferred from mutant phenotype PubMed 10915565. Source: MGI sensory perception of sound Inferred from mutant phenotype PubMed 11545230. Source: MGI skeletal muscle fiber development Inferred from mutant phenotype PubMed 16962942. Source: MGI skeletal muscle tissue regeneration Inferred from mutant phenotype PubMed 16962942. Source: MGI temperature homeostasis Inferred from genetic interaction PubMed 11518697. Source: MGI triglyceride metabolic process Inferred from mutant phenotype PubMed 12117267. Source: MGI vasodilation Inferred from mutant phenotype PubMed 11893559PubMed 12196344. Source: MGI
Cellular_component	mitochondrion Inferred from direct assay PubMed 14651853PubMed 18614015PubMed 9126277. Source: MGI
Molecular_function	endopeptidase inhibitor activity Inferred from electronic annotation. Source: Compara glutathione peroxidase activity Inferred from direct assay PubMed 12521604PubMed 12646716. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Glutathione peroxidase 1		PRO_0000066613
Sites
	Active site	47	1
	Site	47	1	Subject to oxidation and hydroselenide loss to dehydroalanine
Amino acid modifications
	Non-standard residue	47	1	Selenocysteine
	Modified residue	146	1	N6-acetyllysine Ref.5
	Modified residue	201	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P11352 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 401D065165D8AF5C	FASTA	201	22,329
	10 20 30 40 50 60 MCAARLSAAA QSTVYAFSAR PLTGGEPVSL GSLRGKVLLI ENVASLUGTT IRDYTEMNDL 70 80 90 100 110 120 QKRLGPRGLV VLGFPCNQFG HQENGKNEEI LNSLKYVRPG GGFEPNFTLF EKCEVNGEKA 130 140 150 160 170 180 HPLFTFLRNA LPTPSDDPTA LMTDPKYIIW SPVCRNDIAW NFEKFLVGPD GVPVRRYSRR 190 200 FRTIDIEPDI ETLLSQQSGN S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The structure of the mouse glutathione peroxidase gene: the selenocysteine in the active site is encoded by the 'termination' codon, TGA." Chambers I., Frampton J., Goldfarb P., Affara N., McBain W., Harrison P.R. EMBO J. 5:1221-1227(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SELENOCYSTEINE AT SEC-47. Tissue: Erythrocyte.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Bone marrow, Embryonic liver, Kidney and Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"A human cDNA sequence for a novel glutathione peroxidase-related selenopeptide, GPRP." Dunn D.K., Howells D.D., Richardson J., Goldfarb P.S. Nucleic Acids Res. 17:6390-6390(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[5]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-146, MASS SPECTROMETRY. Tissue: Liver.
[6]	"SelT, SelW, SelH, and Rdx12: genomics and molecular insights into the functions of selenoproteins of a novel thioredoxin-like family." Dikiy A., Novoselov S.V., Fomenko D.E., Sengupta A., Carlson B.A., Cerny R.L., Ginalski K., Grishin N.V., Hatfield D.L., Gladyshev V.N. Biochemistry 46:6871-6882(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MIEN1.
[7]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Knockout of SOD1 promotes conversion of selenocysteine to dehydroalanine in murine hepatic GPX1 protein." Wang S.K., Weaver J.D., Zhang S., Lei X.G. Free Radic. Biol. Med. 51:197-204(2011) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, SELENOCYSTEINE AT SEC-47, PARTIAL LOSS OF SELENIUM IN ABSENCE OF SOD1, IDENTIFICATION BY MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03920 Genomic DNA. Translation: CAA27558.1. AK002245 mRNA. Translation: BAC55244.1. AK010999 mRNA. Translation: BAC55252.1. AK011019 mRNA. Translation: BAC55253.1. AK028171 mRNA. Translation: BAC55257.1. AK150548 mRNA. Translation: BAE29650.1. AK154833 mRNA. Translation: BAE32862.1. AK160388 mRNA. Translation: BAE35760.1. BC086649 mRNA. Translation: AAH86649.1. X15667 mRNA. Translation: CAB43535.1. Sequence problems.
IPI	IPI00319652.
PIR	OPMSE. A25106. S05317.
RefSeq	NP_032186.2. NM_008160.6.
UniGene	Mm.1090.
3D structure databases
ProteinModelPortal	P11352.
SMR	P11352. Positions 13-195.
ModBase	Search...
Protein-protein interaction databases
IntAct	P11352. 1 interaction.
MINT	MINT-1855025.
Protein family/group databases
PeroxiBase	3709. MmGPx01.
PTM databases
PhosphoSite	P11352.
2D gel databases
REPRODUCTION-2DPAGE	IPI00319652. P11352.
SWISS-2DPAGE	P11352.
Proteomic databases
PaxDb	P11352.
PRIDE	P11352.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000082429; ENSMUSP00000081010; ENSMUSG00000063856.
GeneID	14775.
KEGG	mmu:14775.
UCSC	uc009rpf.3. mouse.
Organism-specific databases
CTD	2876.
MGI	MGI:104887. Gpx1.
Phylogenomic databases
eggNOG	COG0386.
GeneTree	ENSGT00550000074312.
HOGENOM	HOG000277055.
HOVERGEN	HBG004333.
InParanoid	P11352.
KO	K00432.
OMA	CEVNGAG.
OrthoDB	EOG4SBF08.
Gene expression databases
Bgee	P11352.
CleanEx	MM_GPX1.
Genevestigator	P11352.
GermOnline	ENSMUSG00000063856. Mus musculus.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11592. PTHR11592. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GPX1. mouse.
NextBio	286877.
SOURCE	Search...

Entry information

Entry name

GPX1_MOUSE

Accession

Primary (citable) accession number: P11352
Secondary accession number(s): P12079 , Q544W3, Q5RJH8, Q9CR54

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 26, 2008
Last modified:	May 29, 2013
This is version 136 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program