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P11350

- NARI_ECOLI

UniProt

P11350 - NARI_ECOLI

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Protein

Respiratory nitrate reductase 1 gamma chain

Gene
narI, chlI, b1227, JW1218
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 2 heme groups per subunit. Heme 1, called the proximal or heme Bp in 1 Publication, is located at the cytoplasmic interface, heme 2, called the distal or heme Bd, is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron (heme B 1 axial ligand)
Metal bindingi66 – 661Iron (heme B 2 axial ligand)
Metal bindingi187 – 1871Iron (heme B 2 axial ligand)
Metal bindingi205 – 2051Iron (heme B 1 axial ligand)

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. anaerobic respiration Source: EcoCyc
  2. cytochrome complex assembly Source: EcoCyc
  3. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:NARI-MONOMER.
ECOL316407:JW1218-MONOMER.
MetaCyc:NARI-MONOMER.

Protein family/group databases

TCDBi5.A.3.1.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase 1 gamma chain (EC:1.7.99.4)
Alternative name(s):
Cytochrome B-NR
Nitrate reductase A subunit gamma
Quinol-nitrate oxidoreductase subunit gamma
Gene namesi
Name:narI
Synonyms:chlI
Ordered Locus Names:b1227, JW1218
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10640. narI.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33Periplasmic1 Publication
Transmembranei4 – 2926Helical; Name=1Add
BLAST
Topological domaini30 – 4718Cytoplasmic1 PublicationAdd
BLAST
Transmembranei48 – 7023Helical; Name=2Add
BLAST
Topological domaini71 – 8212Periplasmic1 PublicationAdd
BLAST
Transmembranei83 – 11230Helical; Name=3Add
BLAST
Topological domaini113 – 12412Cytoplasmic1 PublicationAdd
BLAST
Transmembranei125 – 14824Helical; Name=4Add
BLAST
Topological domaini149 – 18234Periplasmic1 PublicationAdd
BLAST
Transmembranei183 – 19816Helical; Name=5Add
BLAST
Topological domaini199 – 22527Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intrinsic component of membrane Source: EcoCyc
  3. nitrate reductase complex Source: CACAO
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Respiratory nitrate reductase 1 gamma chainPRO_0000096723Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-formylmethionine

Keywords - PTMi

Formylation

Expressioni

Inductioni

By nitrate.

Gene expression databases

GenevestigatoriP11350.

Interactioni

Subunit structurei

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.

Protein-protein interaction databases

DIPiDIP-10313N.
IntActiP11350. 2 interactions.
MINTiMINT-1262739.
STRINGi511145.b1227.

Structurei

Secondary structure

1
225
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 109
Helixi12 – 3019
Helixi32 – 343
Turni41 – 433
Helixi48 – 7124
Turni74 – 807
Helixi83 – 11432
Helixi116 – 1216
Helixi124 – 14724
Helixi148 – 1503
Turni151 – 1533
Helixi154 – 16714
Helixi173 – 1775
Helixi182 – 19716
Helixi198 – 2003
Helixi202 – 2087
Helixi211 – 2155
Beta strandi218 – 2225

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q16X-ray1.90C1-225[»]
1SIWX-ray2.20C1-225[»]
1Y4ZX-ray2.00C1-225[»]
1Y5IX-ray1.90C1-225[»]
1Y5LX-ray2.50C1-225[»]
1Y5NX-ray2.50C1-225[»]
3EGWX-ray1.90C1-225[»]
3IR5X-ray2.30C1-225[»]
3IR6X-ray2.80C1-225[»]
3IR7X-ray2.50C1-225[»]
ProteinModelPortaliP11350.
SMRiP11350. Positions 2-225.

Miscellaneous databases

EvolutionaryTraceiP11350.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2181.
HOGENOMiHOG000237376.
KOiK00374.
OMAiMIFPFTR.
OrthoDBiEOG6K13W6.
PhylomeDBiP11350.

Family and domain databases

InterProiIPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view]
PfamiPF02665. Nitrate_red_gam. 1 hit.
[Graphical view]
SUPFAMiSSF103501. SSF103501. 1 hit.
TIGRFAMsiTIGR00351. narI. 1 hit.

Sequencei

Sequence statusi: Complete.

P11350-1 [UniParc]FASTAAdd to Basket

« Hide

MQFLNMFFFD IYPYIAGAVF LIGSWLRYDY GQYTWRAASS QMLDRKGMNL    50
ASNLFHIGIL GIFVGHFFGM LTPHWMYEAW LPIEVKQKMA MFAGGASGVL 100
CLIGGVLLLK RRLFSPRVRA TTTGADILIL SLLVIQCALG LLTIPFSAQH 150
MDGSEMMKLV GWAQSVVTFH GGASQHLDGV AFIFRLHLVL GMTLFLLFPF 200
SRLIHIWSVP VEYLTRKYQL VRARH 225
Length:225
Mass (Da):25,497
Last modified:July 1, 1989 - v1
Checksum:iA0D7989D00D05B72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20147 Genomic DNA. Translation: AAA24197.1.
U00096 Genomic DNA. Translation: AAC74311.1.
AP009048 Genomic DNA. Translation: BAA36097.1.
PIRiC27737. RDECNG.
RefSeqiNP_415745.1. NC_000913.3.
YP_489497.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74311; AAC74311; b1227.
BAA36097; BAA36097; BAA36097.
GeneIDi12933239.
945808.
KEGGiecj:Y75_p1202.
eco:b1227.
PATRICi32117714. VBIEscCol129921_1279.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20147 Genomic DNA. Translation: AAA24197.1 .
U00096 Genomic DNA. Translation: AAC74311.1 .
AP009048 Genomic DNA. Translation: BAA36097.1 .
PIRi C27737. RDECNG.
RefSeqi NP_415745.1. NC_000913.3.
YP_489497.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q16 X-ray 1.90 C 1-225 [» ]
1SIW X-ray 2.20 C 1-225 [» ]
1Y4Z X-ray 2.00 C 1-225 [» ]
1Y5I X-ray 1.90 C 1-225 [» ]
1Y5L X-ray 2.50 C 1-225 [» ]
1Y5N X-ray 2.50 C 1-225 [» ]
3EGW X-ray 1.90 C 1-225 [» ]
3IR5 X-ray 2.30 C 1-225 [» ]
3IR6 X-ray 2.80 C 1-225 [» ]
3IR7 X-ray 2.50 C 1-225 [» ]
ProteinModelPortali P11350.
SMRi P11350. Positions 2-225.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10313N.
IntActi P11350. 2 interactions.
MINTi MINT-1262739.
STRINGi 511145.b1227.

Protein family/group databases

TCDBi 5.A.3.1.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74311 ; AAC74311 ; b1227 .
BAA36097 ; BAA36097 ; BAA36097 .
GeneIDi 12933239.
945808.
KEGGi ecj:Y75_p1202.
eco:b1227.
PATRICi 32117714. VBIEscCol129921_1279.

Organism-specific databases

EchoBASEi EB0634.
EcoGenei EG10640. narI.

Phylogenomic databases

eggNOGi COG2181.
HOGENOMi HOG000237376.
KOi K00374.
OMAi MIFPFTR.
OrthoDBi EOG6K13W6.
PhylomeDBi P11350.

Enzyme and pathway databases

BioCyci EcoCyc:NARI-MONOMER.
ECOL316407:JW1218-MONOMER.
MetaCyc:NARI-MONOMER.

Miscellaneous databases

EvolutionaryTracei P11350.
PROi P11350.

Gene expression databases

Genevestigatori P11350.

Family and domain databases

InterProi IPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view ]
Pfami PF02665. Nitrate_red_gam. 1 hit.
[Graphical view ]
SUPFAMi SSF103501. SSF103501. 1 hit.
TIGRFAMsi TIGR00351. narI. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes."
    Sodergren E.J., Demoss J.A.
    J. Bacteriol. 170:1721-1729(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli."
    Sodergren E.J., Hsu P.Y., Demoss J.A.
    J. Biol. Chem. 263:16156-16162(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-9, FORMYLATION AT MET-1.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A."
    Bertero M.G., Rothery R.A., Palak M., Hou C., Lim D., Blasco F., Weiner J.H., Strynadka N.C.J.
    Nat. Struct. Biol. 10:681-687(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), METAL BINDING AT HIS-56; HIS-66; HIS-187 AND HIS-205.

Entry informationi

Entry nameiNARI_ECOLI
AccessioniPrimary (citable) accession number: P11350
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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