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P11350 (NARI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Respiratory nitrate reductase 1 gamma chain

EC=1.7.99.4
Alternative name(s):
Cytochrome B-NR
Nitrate reductase A subunit gamma
Quinol-nitrate oxidoreductase subunit gamma
Gene names
Name:narI
Synonyms:chlI
Ordered Locus Names:b1227, JW1218
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length225 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 2 heme groups per subunit. Heme 1, called the proximal or heme Bp in Ref.7, is located at the cytoplasmic interface, heme 2, called the distal or heme Bd, is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme.

Subunit structure

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Induction

By nitrate.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 225225Respiratory nitrate reductase 1 gamma chain
PRO_0000096723

Regions

Topological domain1 – 33Periplasmic Ref.6
Transmembrane4 – 2926Helical; Name=1
Topological domain30 – 4718Cytoplasmic Ref.6
Transmembrane48 – 7023Helical; Name=2
Topological domain71 – 8212Periplasmic Ref.6
Transmembrane83 – 11230Helical; Name=3
Topological domain113 – 12412Cytoplasmic Ref.6
Transmembrane125 – 14824Helical; Name=4
Topological domain149 – 18234Periplasmic Ref.6
Transmembrane183 – 19816Helical; Name=5
Topological domain199 – 22527Cytoplasmic Ref.6

Sites

Metal binding561Iron (heme B 1 axial ligand)
Metal binding661Iron (heme B 2 axial ligand)
Metal binding1871Iron (heme B 2 axial ligand)
Metal binding2051Iron (heme B 1 axial ligand)

Amino acid modifications

Modified residue11N-formylmethionine

Secondary structure

................................. 225
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P11350 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: A0D7989D00D05B72

FASTA22525,497
        10         20         30         40         50         60 
MQFLNMFFFD IYPYIAGAVF LIGSWLRYDY GQYTWRAASS QMLDRKGMNL ASNLFHIGIL 

        70         80         90        100        110        120 
GIFVGHFFGM LTPHWMYEAW LPIEVKQKMA MFAGGASGVL CLIGGVLLLK RRLFSPRVRA 

       130        140        150        160        170        180 
TTTGADILIL SLLVIQCALG LLTIPFSAQH MDGSEMMKLV GWAQSVVTFH GGASQHLDGV 

       190        200        210        220 
AFIFRLHLVL GMTLFLLFPF SRLIHIWSVP VEYLTRKYQL VRARH 

« Hide

References

« Hide 'large scale' references
[1]"narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes."
Sodergren E.J., Demoss J.A.
J. Bacteriol. 170:1721-1729(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli."
Sodergren E.J., Hsu P.Y., Demoss J.A.
J. Biol. Chem. 263:16156-16162(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-9, FORMYLATION AT MET-1.
[6]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
[7]"Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A."
Bertero M.G., Rothery R.A., Palak M., Hou C., Lim D., Blasco F., Weiner J.H., Strynadka N.C.J.
Nat. Struct. Biol. 10:681-687(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), METAL BINDING AT HIS-56; HIS-66; HIS-187 AND HIS-205.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20147 Genomic DNA. Translation: AAA24197.1.
U00096 Genomic DNA. Translation: AAC74311.1.
AP009048 Genomic DNA. Translation: BAA36097.1.
PIRRDECNG. C27737.
RefSeqNP_415745.1. NC_000913.3.
YP_489497.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q16X-ray1.90C1-225[»]
1SIWX-ray2.20C1-225[»]
1Y4ZX-ray2.00C1-225[»]
1Y5IX-ray1.90C1-225[»]
1Y5LX-ray2.50C1-225[»]
1Y5NX-ray2.50C1-225[»]
3EGWX-ray1.90C1-225[»]
3IR5X-ray2.30C1-225[»]
3IR6X-ray2.80C1-225[»]
3IR7X-ray2.50C1-225[»]
ProteinModelPortalP11350.
SMRP11350. Positions 2-225.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10313N.
IntActP11350. 2 interactions.
MINTMINT-1262739.
STRING511145.b1227.

Protein family/group databases

TCDB5.A.3.1.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74311; AAC74311; b1227.
BAA36097; BAA36097; BAA36097.
GeneID12933239.
945808.
KEGGecj:Y75_p1202.
eco:b1227.
PATRIC32117714. VBIEscCol129921_1279.

Organism-specific databases

EchoBASEEB0634.
EcoGeneEG10640. narI.

Phylogenomic databases

eggNOGCOG2181.
HOGENOMHOG000237376.
KOK00374.
OMAMIFPFTR.
OrthoDBEOG6K13W6.
PhylomeDBP11350.

Enzyme and pathway databases

BioCycEcoCyc:NARI-MONOMER.
ECOL316407:JW1218-MONOMER.
MetaCyc:NARI-MONOMER.

Gene expression databases

GenevestigatorP11350.

Family and domain databases

InterProIPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view]
PfamPF02665. Nitrate_red_gam. 1 hit.
[Graphical view]
SUPFAMSSF103501. SSF103501. 1 hit.
TIGRFAMsTIGR00351. narI. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP11350.
PROP11350.

Entry information

Entry nameNARI_ECOLI
AccessionPrimary (citable) accession number: P11350
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene