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P11350

- NARI_ECOLI

UniProt

P11350 - NARI_ECOLI

Protein

Respiratory nitrate reductase 1 gamma chain

Gene

narI

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 2 heme groups per subunit. Heme 1, called the proximal or heme Bp in PubMed:12910261, is located at the cytoplasmic interface, heme 2, called the distal or heme Bd, is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Iron (heme B 1 axial ligand)
    Metal bindingi66 – 661Iron (heme B 2 axial ligand)
    Metal bindingi187 – 1871Iron (heme B 2 axial ligand)
    Metal bindingi205 – 2051Iron (heme B 1 axial ligand)

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. anaerobic respiration Source: EcoCyc
    2. cytochrome complex assembly Source: EcoCyc
    3. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:NARI-MONOMER.
    ECOL316407:JW1218-MONOMER.
    MetaCyc:NARI-MONOMER.

    Protein family/group databases

    TCDBi5.A.3.1.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Respiratory nitrate reductase 1 gamma chain (EC:1.7.99.4)
    Alternative name(s):
    Cytochrome B-NR
    Nitrate reductase A subunit gamma
    Quinol-nitrate oxidoreductase subunit gamma
    Gene namesi
    Name:narI
    Synonyms:chlI
    Ordered Locus Names:b1227, JW1218
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10640. narI.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. intrinsic component of membrane Source: EcoCyc
    3. nitrate reductase complex Source: CACAO
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225Respiratory nitrate reductase 1 gamma chainPRO_0000096723Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-formylmethionine1 Publication

    Keywords - PTMi

    Formylation

    Expressioni

    Inductioni

    By nitrate.

    Gene expression databases

    GenevestigatoriP11350.

    Interactioni

    Subunit structurei

    Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.

    Protein-protein interaction databases

    DIPiDIP-10313N.
    IntActiP11350. 2 interactions.
    MINTiMINT-1262739.
    STRINGi511145.b1227.

    Structurei

    Secondary structure

    1
    225
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 109
    Helixi12 – 3019
    Helixi32 – 343
    Turni41 – 433
    Helixi48 – 7124
    Turni74 – 807
    Helixi83 – 11432
    Helixi116 – 1216
    Helixi124 – 14724
    Helixi148 – 1503
    Turni151 – 1533
    Helixi154 – 16714
    Helixi173 – 1775
    Helixi182 – 19716
    Helixi198 – 2003
    Helixi202 – 2087
    Helixi211 – 2155
    Beta strandi218 – 2225

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q16X-ray1.90C1-225[»]
    1SIWX-ray2.20C1-225[»]
    1Y4ZX-ray2.00C1-225[»]
    1Y5IX-ray1.90C1-225[»]
    1Y5LX-ray2.50C1-225[»]
    1Y5NX-ray2.50C1-225[»]
    3EGWX-ray1.90C1-225[»]
    3IR5X-ray2.30C1-225[»]
    3IR6X-ray2.80C1-225[»]
    3IR7X-ray2.50C1-225[»]
    ProteinModelPortaliP11350.
    SMRiP11350. Positions 2-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11350.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 33Periplasmic1 Publication
    Topological domaini30 – 4718Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini71 – 8212Periplasmic1 PublicationAdd
    BLAST
    Topological domaini113 – 12412Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini149 – 18234Periplasmic1 PublicationAdd
    BLAST
    Topological domaini199 – 22527Cytoplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2926Helical; Name=1Add
    BLAST
    Transmembranei48 – 7023Helical; Name=2Add
    BLAST
    Transmembranei83 – 11230Helical; Name=3Add
    BLAST
    Transmembranei125 – 14824Helical; Name=4Add
    BLAST
    Transmembranei183 – 19816Helical; Name=5Add
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2181.
    HOGENOMiHOG000237376.
    KOiK00374.
    OMAiMIFPFTR.
    OrthoDBiEOG6K13W6.
    PhylomeDBiP11350.

    Family and domain databases

    InterProiIPR023234. NarG-like_domain.
    IPR003816. Nitrate_red_gam.
    [Graphical view]
    PfamiPF02665. Nitrate_red_gam. 1 hit.
    [Graphical view]
    SUPFAMiSSF103501. SSF103501. 1 hit.
    TIGRFAMsiTIGR00351. narI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P11350-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQFLNMFFFD IYPYIAGAVF LIGSWLRYDY GQYTWRAASS QMLDRKGMNL    50
    ASNLFHIGIL GIFVGHFFGM LTPHWMYEAW LPIEVKQKMA MFAGGASGVL 100
    CLIGGVLLLK RRLFSPRVRA TTTGADILIL SLLVIQCALG LLTIPFSAQH 150
    MDGSEMMKLV GWAQSVVTFH GGASQHLDGV AFIFRLHLVL GMTLFLLFPF 200
    SRLIHIWSVP VEYLTRKYQL VRARH 225
    Length:225
    Mass (Da):25,497
    Last modified:July 1, 1989 - v1
    Checksum:iA0D7989D00D05B72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20147 Genomic DNA. Translation: AAA24197.1.
    U00096 Genomic DNA. Translation: AAC74311.1.
    AP009048 Genomic DNA. Translation: BAA36097.1.
    PIRiC27737. RDECNG.
    RefSeqiNP_415745.1. NC_000913.3.
    YP_489497.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74311; AAC74311; b1227.
    BAA36097; BAA36097; BAA36097.
    GeneIDi12933239.
    945808.
    KEGGiecj:Y75_p1202.
    eco:b1227.
    PATRICi32117714. VBIEscCol129921_1279.

    Cross-referencesi

    Web resourcesi

    Worthington enzyme manual

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20147 Genomic DNA. Translation: AAA24197.1 .
    U00096 Genomic DNA. Translation: AAC74311.1 .
    AP009048 Genomic DNA. Translation: BAA36097.1 .
    PIRi C27737. RDECNG.
    RefSeqi NP_415745.1. NC_000913.3.
    YP_489497.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q16 X-ray 1.90 C 1-225 [» ]
    1SIW X-ray 2.20 C 1-225 [» ]
    1Y4Z X-ray 2.00 C 1-225 [» ]
    1Y5I X-ray 1.90 C 1-225 [» ]
    1Y5L X-ray 2.50 C 1-225 [» ]
    1Y5N X-ray 2.50 C 1-225 [» ]
    3EGW X-ray 1.90 C 1-225 [» ]
    3IR5 X-ray 2.30 C 1-225 [» ]
    3IR6 X-ray 2.80 C 1-225 [» ]
    3IR7 X-ray 2.50 C 1-225 [» ]
    ProteinModelPortali P11350.
    SMRi P11350. Positions 2-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10313N.
    IntActi P11350. 2 interactions.
    MINTi MINT-1262739.
    STRINGi 511145.b1227.

    Protein family/group databases

    TCDBi 5.A.3.1.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74311 ; AAC74311 ; b1227 .
    BAA36097 ; BAA36097 ; BAA36097 .
    GeneIDi 12933239.
    945808.
    KEGGi ecj:Y75_p1202.
    eco:b1227.
    PATRICi 32117714. VBIEscCol129921_1279.

    Organism-specific databases

    EchoBASEi EB0634.
    EcoGenei EG10640. narI.

    Phylogenomic databases

    eggNOGi COG2181.
    HOGENOMi HOG000237376.
    KOi K00374.
    OMAi MIFPFTR.
    OrthoDBi EOG6K13W6.
    PhylomeDBi P11350.

    Enzyme and pathway databases

    BioCyci EcoCyc:NARI-MONOMER.
    ECOL316407:JW1218-MONOMER.
    MetaCyc:NARI-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P11350.
    PROi P11350.

    Gene expression databases

    Genevestigatori P11350.

    Family and domain databases

    InterProi IPR023234. NarG-like_domain.
    IPR003816. Nitrate_red_gam.
    [Graphical view ]
    Pfami PF02665. Nitrate_red_gam. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103501. SSF103501. 1 hit.
    TIGRFAMsi TIGR00351. narI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes."
      Sodergren E.J., Demoss J.A.
      J. Bacteriol. 170:1721-1729(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli."
      Sodergren E.J., Hsu P.Y., Demoss J.A.
      J. Biol. Chem. 263:16156-16162(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-9, FORMYLATION AT MET-1.
    6. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.
    7. "Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A."
      Bertero M.G., Rothery R.A., Palak M., Hou C., Lim D., Blasco F., Weiner J.H., Strynadka N.C.J.
      Nat. Struct. Biol. 10:681-687(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), METAL BINDING AT HIS-56; HIS-66; HIS-187 AND HIS-205.

    Entry informationi

    Entry nameiNARI_ECOLI
    AccessioniPrimary (citable) accession number: P11350
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3