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Protein

Nitrogenase molybdenum-iron protein beta chain

Gene

nifK

Organism
Clostridium pasteurianum
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[8Fe-7S] clusterNote: Binds 1 [8Fe-7S] cluster per heterodimer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23Iron-sulfur (8Fe-7S); shared with alpha chain1
Metal bindingi48Iron-sulfur (8Fe-7S); shared with alpha chain1
Metal bindingi106Iron-sulfur (8Fe-7S); shared with alpha chain1
Metal bindingi141Iron-sulfur (8Fe-7S); shared with alpha chain1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:NIFKCP-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein beta chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifK
OrganismiClostridium pasteurianum
Taxonomic identifieri1501 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001530971 – 458Nitrogenase molybdenum-iron protein beta chainAdd BLAST458

Proteomic databases

PRIDEiP11347.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 20Combined sources5
Helixi24 – 33Combined sources10
Beta strandi38 – 44Combined sources7
Helixi46 – 60Combined sources15
Helixi73 – 78Combined sources6
Helixi81 – 95Combined sources15
Beta strandi98 – 104Combined sources7
Helixi106 – 111Combined sources6
Helixi115 – 125Combined sources11
Beta strandi133 – 136Combined sources4
Helixi146 – 161Combined sources16
Beta strandi172 – 175Combined sources4
Helixi181 – 193Combined sources13
Beta strandi198 – 202Combined sources5
Turni205 – 207Combined sources3
Helixi226 – 231Combined sources6
Helixi232 – 234Combined sources3
Beta strandi236 – 242Combined sources7
Helixi243 – 257Combined sources15
Beta strandi261 – 264Combined sources4
Helixi270 – 284Combined sources15
Helixi290 – 305Combined sources16
Helixi307 – 309Combined sources3
Turni310 – 312Combined sources3
Beta strandi314 – 319Combined sources6
Helixi321 – 333Combined sources13
Beta strandi337 – 345Combined sources9
Helixi348 – 360Combined sources13
Beta strandi367 – 370Combined sources4
Helixi374 – 383Combined sources10
Beta strandi387 – 392Combined sources6
Helixi393 – 395Combined sources3
Helixi396 – 402Combined sources7
Beta strandi406 – 408Combined sources3
Helixi419 – 421Combined sources3
Helixi426 – 448Combined sources23
Helixi451 – 453Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MIOX-ray3.00B/D1-458[»]
4WESX-ray1.08B/D1-458[»]
4WN9X-ray1.90B/D1-458[»]
ProteinModelPortaliP11347.
SMRiP11347.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11347.

Family & Domainsi

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.Curated

Family and domain databases

InterProiIPR000510. Nase/OxRdtase_comp1.
IPR000318. Nase_comp1_CS.
IPR005976. Nase_Mo-Fe_CF_bsu.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01286. nifK. 1 hit.
PROSITEiPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDATPKEIV ERKALRINPA KTCQPVGAMY AALGIHNCLP HSHGSQGCCS
60 70 80 90 100
YHRTVLSRHF KEPAMASTSS FTEGASVFGG GSNIKTAVKN IFSLYNPDII
110 120 130 140 150
AVHTTCLSET LGDDLPTYIS QMEDAGSIPE GKLVIHTNTP SYVGSHVTGF
160 170 180 190 200
ANMVQGIVNY LSENTGAKNG KINVIPGFVG PADMREIKRL FEAMDIPYIM
210 220 230 240 250
FPDTSGVLDG PTTGEYKMYP EGGTKIEDLK DTGNSDLTLS LGSYASDLGA
260 270 280 290 300
KTLEKKCKVP FKTLRTPIGV SATDEFIMAL SEATGKEVPA SIEEERGQLI
310 320 330 340 350
DLMIDAQQYL QGKKVALLGD PDEIIALSKF IIELGAIPKY VVTGTPGMKF
360 370 380 390 400
QKEIDAMLAE AGIEGSKVKV EGDFFDVHQW IKNEGVDLLI SNTYGKFIAR
410 420 430 440 450
EENIPFVRFG FPIMDRYGHY YNPKVGYKGA IRLVEEITNV ILDKIERECT

EEDFEVVR
Length:458
Mass (Da):50,119
Last modified:July 1, 1989 - v1
Checksum:iFECFB097C7CC9068
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY603957 Genomic DNA. Translation: AAA23266.2.
PIRiA29992. NICLMB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY603957 Genomic DNA. Translation: AAA23266.2.
PIRiA29992. NICLMB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MIOX-ray3.00B/D1-458[»]
4WESX-ray1.08B/D1-458[»]
4WN9X-ray1.90B/D1-458[»]
ProteinModelPortaliP11347.
SMRiP11347.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP11347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:NIFKCP-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP11347.

Family and domain databases

InterProiIPR000510. Nase/OxRdtase_comp1.
IPR000318. Nase_comp1_CS.
IPR005976. Nase_Mo-Fe_CF_bsu.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01286. nifK. 1 hit.
PROSITEiPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIFK_CLOPA
AccessioniPrimary (citable) accession number: P11347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.