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Protein

Nitrogenase molybdenum-iron protein beta chain

Gene

nifK

Organism
Clostridium pasteurianum
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[8Fe-7S] clusterNote: Binds 1 [8Fe-7S] cluster per heterodimer.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Iron-sulfur (8Fe-7S); shared with alpha chain
Metal bindingi48 – 481Iron-sulfur (8Fe-7S); shared with alpha chain
Metal bindingi106 – 1061Iron-sulfur (8Fe-7S); shared with alpha chain
Metal bindingi141 – 1411Iron-sulfur (8Fe-7S); shared with alpha chain

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:NIFKCP-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein beta chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifK
OrganismiClostridium pasteurianum
Taxonomic identifieri1501 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458Nitrogenase molybdenum-iron protein beta chainPRO_0000153097Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Structurei

Secondary structure

1
458
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 205Combined sources
Helixi24 – 3310Combined sources
Beta strandi38 – 447Combined sources
Helixi46 – 6015Combined sources
Helixi73 – 786Combined sources
Helixi81 – 9515Combined sources
Beta strandi98 – 1047Combined sources
Helixi106 – 1116Combined sources
Helixi115 – 12511Combined sources
Beta strandi133 – 1364Combined sources
Helixi146 – 16116Combined sources
Beta strandi172 – 1754Combined sources
Helixi181 – 19313Combined sources
Beta strandi198 – 2025Combined sources
Turni205 – 2073Combined sources
Helixi226 – 2316Combined sources
Helixi232 – 2343Combined sources
Beta strandi236 – 2427Combined sources
Helixi243 – 25715Combined sources
Beta strandi261 – 2644Combined sources
Helixi270 – 28415Combined sources
Helixi290 – 30516Combined sources
Helixi307 – 3093Combined sources
Turni310 – 3123Combined sources
Beta strandi314 – 3196Combined sources
Helixi321 – 33313Combined sources
Beta strandi337 – 3459Combined sources
Helixi348 – 36013Combined sources
Beta strandi367 – 3704Combined sources
Helixi374 – 38310Combined sources
Beta strandi387 – 3926Combined sources
Helixi393 – 3953Combined sources
Helixi396 – 4027Combined sources
Beta strandi406 – 4083Combined sources
Helixi419 – 4213Combined sources
Helixi426 – 44823Combined sources
Helixi451 – 4533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MIOX-ray3.00B/D1-458[»]
4WESX-ray1.08B/D1-458[»]
4WN9X-ray1.90B/D1-458[»]
ProteinModelPortaliP11347.
SMRiP11347. Positions 2-458.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11347.

Family & Domainsi

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.Curated

Family and domain databases

InterProiIPR000510. Nase/OxRdtase_comp1.
IPR000318. Nase_comp1_CS.
IPR005976. Nase_Mo-Fe_CF_bsu.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01286. nifK. 1 hit.
PROSITEiPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDATPKEIV ERKALRINPA KTCQPVGAMY AALGIHNCLP HSHGSQGCCS
60 70 80 90 100
YHRTVLSRHF KEPAMASTSS FTEGASVFGG GSNIKTAVKN IFSLYNPDII
110 120 130 140 150
AVHTTCLSET LGDDLPTYIS QMEDAGSIPE GKLVIHTNTP SYVGSHVTGF
160 170 180 190 200
ANMVQGIVNY LSENTGAKNG KINVIPGFVG PADMREIKRL FEAMDIPYIM
210 220 230 240 250
FPDTSGVLDG PTTGEYKMYP EGGTKIEDLK DTGNSDLTLS LGSYASDLGA
260 270 280 290 300
KTLEKKCKVP FKTLRTPIGV SATDEFIMAL SEATGKEVPA SIEEERGQLI
310 320 330 340 350
DLMIDAQQYL QGKKVALLGD PDEIIALSKF IIELGAIPKY VVTGTPGMKF
360 370 380 390 400
QKEIDAMLAE AGIEGSKVKV EGDFFDVHQW IKNEGVDLLI SNTYGKFIAR
410 420 430 440 450
EENIPFVRFG FPIMDRYGHY YNPKVGYKGA IRLVEEITNV ILDKIERECT

EEDFEVVR
Length:458
Mass (Da):50,119
Last modified:July 1, 1989 - v1
Checksum:iFECFB097C7CC9068
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY603957 Genomic DNA. Translation: AAA23266.2.
PIRiA29992. NICLMB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY603957 Genomic DNA. Translation: AAA23266.2.
PIRiA29992. NICLMB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MIOX-ray3.00B/D1-458[»]
4WESX-ray1.08B/D1-458[»]
4WN9X-ray1.90B/D1-458[»]
ProteinModelPortaliP11347.
SMRiP11347. Positions 2-458.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:NIFKCP-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP11347.

Family and domain databases

InterProiIPR000510. Nase/OxRdtase_comp1.
IPR000318. Nase_comp1_CS.
IPR005976. Nase_Mo-Fe_CF_bsu.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01286. nifK. 1 hit.
PROSITEiPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Distinct structural features of the alpha and beta subunits of nitrogenase molybdenum-iron protein of Clostridium pasteurianum: an analysis of amino acid sequences."
    Wang S.-Z., Chen J.-S., Johnson J.L.
    Biochemistry 27:2800-2810(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 6013 / DSM 525 / NCIB 9486 / VKM B-1774 / W5.
  2. "Nucleotide and deduced amino acid sequences of nifD encoding the alpha-subunit of nitrogenase MoFe protein of Clostridium pasteurianum."
    Wang S.-Z., Chen J.-S., Johnson J.L.
    Nucleic Acids Res. 15:3935-3935(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
  3. "X-ray crystal structure of the nitrogenase molybdenum-iron protein from Clostridium pasteurianum at 3.0-A resolution."
    Kim J., Woo D., Rees D.C.
    Biochemistry 32:7104-7115(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Entry informationi

Entry nameiNIFK_CLOPA
AccessioniPrimary (citable) accession number: P11347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: December 9, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.