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P11344

- TYRO_MOUSE

UniProt

P11344 - TYRO_MOUSE

Protein

Tyrosinase

Gene

Tyr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 3 (29 May 2007)
      Previous versions | rss
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    Functioni

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.

    Catalytic activityi

    2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
    L-tyrosine + O2 = dopaquinone + H2O.

    Cofactori

    Binds 2 copper ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi180 – 1801Copper ABy similarity
    Metal bindingi202 – 2021Copper ABy similarity
    Metal bindingi211 – 2111Copper ABy similarity
    Metal bindingi363 – 3631Copper BBy similarity
    Metal bindingi367 – 3671Copper BBy similarity
    Metal bindingi390 – 3901Copper BBy similarity

    GO - Molecular functioni

    1. copper ion binding Source: Ensembl
    2. monophenol monooxygenase activity Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. cell proliferation Source: MGI
    2. melanin biosynthetic process Source: MGI
    3. pigmentation Source: MGI
    4. thymus development Source: MGI

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosinase (EC:1.14.18.1)
    Alternative name(s):
    Albino locus protein
    Monophenol monooxygenase
    Gene namesi
    Name:Tyr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:98880. Tyr.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB
    2. melanosome Source: UniProtKB
    3. melanosome membrane Source: UniProtKB-SubCell
    4. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Tyr result in various forms of albinism. Himalayan strain tyrosinase is temperature-sensitive.

    Keywords - Diseasei

    Albinism, Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 533515TyrosinasePRO_0000035880Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP11344.

    PTM databases

    PhosphoSiteiP11344.

    Expressioni

    Gene expression databases

    BgeeiP11344.
    CleanExiMM_TYR.
    GenevestigatoriP11344.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Tyrp1P071472EBI-821603,EBI-821614

    Protein-protein interaction databases

    BioGridi204394. 7 interactions.
    IntActiP11344. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP11344.
    SMRiP11344. Positions 172-449.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 476458Lumenal, melanosomeSequence AnalysisAdd
    BLAST
    Topological domaini498 – 53336CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei477 – 49721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi503 – 5086Poly-Lys

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG08919.
    HOGENOMiHOG000118376.
    HOVERGENiHBG003553.
    InParanoidiP11344.
    KOiK00505.
    OMAiCLSLTQY.
    OrthoDBiEOG7TJ3HG.
    PhylomeDBiP11344.
    TreeFamiTF315865.

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PfamiPF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 2 hits.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11344-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLAVLYCLL WSFQISDGHF PRACASSKNL LAKECCPPWM GDGSPCGQLS    50
    GRGSCQDILL SSAPSGPQFP FKGVDDRESW PSVFYNRTCQ CSGNFMGFNC 100
    GNCKFGFGGP NCTEKRVLIR RNIFDLSVSE KNKFFSYLTL AKHTISSVYV 150
    IPTGTYGQMN NGSTPMFNDI NIYDLFVWMH YYVSRDTLLG GSEIWRDIDF 200
    AHEAPGFLPW HRLFLLLWEQ EIRELTGDEN FTVPYWDWRD AENCDICTDE 250
    YLGGRHPENP NLLSPASFFS SWQIICSRSE EYNSHQVLCD GTPEGPLLRN 300
    PGNHDKAKTP RLPSSADVEF CLSLTQYESG SMDRTANFSF RNTLEGFASP 350
    LTGIADPSQS SMHNALHIFM NGTMSQVQGS ANDPIFLLHH AFVDSIFEQW 400
    LRRHRPLLEV YPEANAPIGH NRDSYMVPFI PLYRNGDFFI TSKDLGYDYS 450
    YLQESDPGFY RNYIEPYLEQ ASRIWPWLLG AALVGAVIAA ALSGLSSRLC 500
    LQKKKKKKQP QEERQPLLMD KDDYHSLLYQ SHL 533
    Length:533
    Mass (Da):60,606
    Last modified:May 29, 2007 - v3
    Checksum:iA4C109A97CBD5D6A
    GO

    Sequence cautioni

    The sequence BAA00079.1 differs from that shown. Reason:

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401M → I in AAA37806. (PubMed:2567165)Curated
    Sequence conflicti197 – 1971D → Q in AAA37806. (PubMed:2567165)Curated
    Sequence conflicti264 – 2641S → I in CAA31273. (PubMed:3141148)Curated
    Sequence conflicti346 – 3461G → V in AAA40516. (PubMed:3134020)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti103 – 1031C → S in albino mice. 3 Publications
    Natural varianti420 – 4201H → R in strain: Himalayan. 1 Publication
    Natural varianti482 – 4821A → T in chinchilla mice. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00440 mRNA. Translation: BAA00341.1.
    D00131 mRNA. Translation: BAA00079.1. Sequence problems.
    M20234 mRNA. Translation: AAA40516.1.
    X12782 mRNA. Translation: CAA31273.1.
    M26729 mRNA. Translation: AAA37806.1.
    M24560 mRNA. Translation: AAA40517.1.
    D00439 Genomic DNA. Translation: BAA00340.1.
    X51743 Genomic DNA. Translation: CAA36033.1.
    CCDSiCCDS52304.1.
    PIRiA27711. YRMSCS.
    RefSeqiNP_035791.1. NM_011661.4.
    UniGeneiMm.238127.

    Genome annotation databases

    EnsembliENSMUST00000004770; ENSMUSP00000004770; ENSMUSG00000004651.
    GeneIDi22173.
    KEGGimmu:22173.
    UCSCiuc009ifo.1. mouse.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Snowy stardom - Issue 49 of August 2004

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00440 mRNA. Translation: BAA00341.1 .
    D00131 mRNA. Translation: BAA00079.1 . Sequence problems.
    M20234 mRNA. Translation: AAA40516.1 .
    X12782 mRNA. Translation: CAA31273.1 .
    M26729 mRNA. Translation: AAA37806.1 .
    M24560 mRNA. Translation: AAA40517.1 .
    D00439 Genomic DNA. Translation: BAA00340.1 .
    X51743 Genomic DNA. Translation: CAA36033.1 .
    CCDSi CCDS52304.1.
    PIRi A27711. YRMSCS.
    RefSeqi NP_035791.1. NM_011661.4.
    UniGenei Mm.238127.

    3D structure databases

    ProteinModelPortali P11344.
    SMRi P11344. Positions 172-449.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204394. 7 interactions.
    IntActi P11344. 1 interaction.

    Chemistry

    BindingDBi P11344.
    ChEMBLi CHEMBL5346.

    PTM databases

    PhosphoSitei P11344.

    Proteomic databases

    PRIDEi P11344.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004770 ; ENSMUSP00000004770 ; ENSMUSG00000004651 .
    GeneIDi 22173.
    KEGGi mmu:22173.
    UCSCi uc009ifo.1. mouse.

    Organism-specific databases

    CTDi 7299.
    MGIi MGI:98880. Tyr.

    Phylogenomic databases

    eggNOGi NOG08919.
    HOGENOMi HOG000118376.
    HOVERGENi HBG003553.
    InParanoidi P11344.
    KOi K00505.
    OMAi CLSLTQY.
    OrthoDBi EOG7TJ3HG.
    PhylomeDBi P11344.
    TreeFami TF315865.

    Miscellaneous databases

    NextBioi 302123.
    PROi P11344.
    SOURCEi Search...

    Gene expression databases

    Bgeei P11344.
    CleanExi MM_TYR.
    Genevestigatori P11344.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    Pfami PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 2 hits.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of mouse tyrosinase cDNA."
      Yamamoto H., Takeuchi S., Kudo T., Makino K., Nakata A., Shinoda T., Takeuchi T.
      Jpn. J. Genet. 62:271-274(1987)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    2. "Sequence analysis of mouse tyrosinase cDNA and the effect of melanotropin on its gene expression."
      Kwon B.S., Wakulchik M., Haq A.K., Halaban R., Kestler D.
      Biochem. Biophys. Res. Commun. 153:1301-1309(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-103.
      Strain: DBA/2J.
    3. "Functional analysis of alternatively spliced tyrosinase gene transcripts."
      Mueller G., Ruppert S., Schmid E., Schuetz G.
      EMBO J. 7:2723-2730(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-420.
      Strain: Himalayan.
    5. "Isolation and characterization of variant cDNAs encoding mouse tyrosinase."
      Terao M., Tabe L., Garattini E., Sartori D., Studer M., Mintz B.
      Biochem. Biophys. Res. Commun. 159:848-853(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    6. "Melanin production in cultured albino melanocytes transfected with mouse tyrosinase cDNA."
      Yamamoto H., Takeuchi S., Kudo T., Sato C., Takeuchi T.
      Jpn. J. Genet. 64:121-135(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-273.
    7. Cited for: NUCLEOTIDE SEQUENCE OF 1-273, VARIANT ALBINO SER-103.
      Strain: BALB/c.
    8. "A point mutation in the tyrosinase gene of BALB/c albino mouse causing the cysteine-->serine substitution at position 85."
      Shibahara S., Okinaga S., Tomita Y., Takeda A., Yamamoto H., Sato M., Takeuchi T.
      Eur. J. Biochem. 189:455-461(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13, VARIANT ALBINO SER-103.
      Strain: BALB/c.
    9. "Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice."
      Beermann F., Ruppert S., Hummler E., Bosch F.X., Mueller G., Ruether U., Schuetz G.
      EMBO J. 9:2819-2826(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT CHINCHILLA MICE THR-482.

    Entry informationi

    Entry nameiTYRO_MOUSE
    AccessioniPrimary (citable) accession number: P11344
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 149 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3