P11325 (SYLM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase, mitochondrial
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	NAM2
Synonyms:	MSL1
Ordered Locus Names:	YLR382C
ORF Names:	L3502.6

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).
Subcellular location	Mitochondrion matrix.
Miscellaneous	Ref.2 authors identified this protein as the gene product of the NAM2 gene, which is capable of compensating for mutations in mRNA maturase encoded by the fourth intron of the mitochondrial cytochrome b gene. Present with 2120 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Group I intron splicing Inferred from mutant phenotype PubMed 2277640. Source: SGD leucyl-tRNA aminoacylation Inferred from direct assay Ref.5. Source: SGD mitochondrial translation Inferred from genetic interaction PubMed 16777356. Source: SGD regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961Ref.5. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from direct assay Ref.5. Source: SGD mRNA binding Inferred from physical interaction PubMed 11142386. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 9	9	Mitochondrion
	Chain	10 – 894	885	Leucine--tRNA ligase, mitochondrial		PRO_0000035810
Regions
	Motif	56 – 66	11	"HIGH" region
	Motif	646 – 650	5	"KMSKS" region
Sites
	Binding site	649	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P11325 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 3862CEFFFB10B262	FASTA	894	101,920
	10 20 30 40 50 60 MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY ILCQFPYPSG 70 80 90 100 110 120 ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA ENAAIERSIN PAIWTRDNIA 130 140 150 160 170 180 KMKQQMQSML ANFDWDREIT TCDPEYYKFT QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL 190 200 210 220 230 240 ANEQVDAQGR SWRSGAIVEK KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG 250 260 270 280 290 300 ESVGAELVFK VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK 310 320 330 340 350 360 SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC PGHDNRDFEF 370 380 390 400 410 420 WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS TDGVLTKECG EHSGVLTVVA 430 440 450 460 470 480 RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ RYWGTPIPII HCDNCGPVPV PESDLPVKLP 490 500 510 520 530 540 ELEGLDTKGN PLSTIDEFVN VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF 550 560 570 580 590 600 DREIASKNMP VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV 610 620 630 640 650 660 QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS KYNGADPNEC 670 680 690 700 710 720 ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV LHLTKNILSL EKDLAISKDY 730 740 750 760 770 780 KTPTDLNDAE VKFHNDFQRF LKSITESFEV NLSLNTVISD YMKLTNILES ALKKGEVRNE 790 800 810 820 830 840 MIVQNLQKLV TVIYPAVPSI SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR 850 860 870 880 890 VKFMYTADKN FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli." Tzagoloff A., Akai A., Kurkulos M., Repetto B. J. Biol. Chem. 263:850-856(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene." Labouesse M., Herbert C.J., Dujardin G., Slonimski P.P. EMBO J. 6:713-721(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AB1-4A/8/55.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase." Zagorski W., Castaing B., Herbert C.J., Labouesse M., Martin R., Slonimski P.P. J. Biol. Chem. 266:2537-2541(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03495 Genomic DNA. Translation: AAA34805.1. X05143 Genomic DNA. Translation: CAA28791.1. U19104 Genomic DNA. Translation: AAB67277.1. BK006945 Genomic DNA. Translation: DAA09683.1.
PIR	SYBYLM. A28521.
RefSeq	NP_013486.3. NM_001182271.3.
3D structure databases
ProteinModelPortal	P11325.
SMR	P11325. Positions 25-803.
ModBase	Search...
Protein-protein interaction databases
IntAct	P11325. 1 interaction.
MINT	MINT-2785917.
STRING	4932.YLR382C.
Proteomic databases
PaxDb	P11325.
PeptideAtlas	P11325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR382C; YLR382C; YLR382C.
GeneID	851098.
KEGG	sce:YLR382C. sce:YLR384C.
Organism-specific databases
CYGD	YLR382c.
SGD	S000004374. NAM2.
Phylogenomic databases
eggNOG	COG0495.
GeneTree	ENSGT00390000015114.
HOGENOM	HOG000200747.
KO	K01869. K11373.
OMA	GWPEQVK.
OrthoDB	EOG48D43H.
Gene expression databases
Genevestigator	P11325.
GermOnline	YLR382C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF00133. tRNA-synt_1. 2 hits. PF13603. tRNA-synt_1_2. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967782.

Entry information

Entry name

SYLM_YEAST

Accession

Primary (citable) accession number: P11325
Secondary accession number(s): D6VZ17

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program