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P11325

- SYLM_YEAST

UniProt

P11325 - SYLM_YEAST

Protein

Leucine--tRNA ligase, mitochondrial

Gene

NAM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei649 – 6491ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: SGD
    4. mRNA binding Source: SGD

    GO - Biological processi

    1. Group I intron splicing Source: SGD
    2. leucyl-tRNA aminoacylation Source: SGD
    3. mitochondrial translation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32448-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:NAM2
    Synonyms:MSL1
    Ordered Locus Names:YLR382C
    ORF Names:L3502.6
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR382c.
    SGDiS000004374. NAM2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 99Mitochondrion
    Chaini10 – 894885Leucine--tRNA ligase, mitochondrialPRO_0000035810Add
    BLAST

    Proteomic databases

    MaxQBiP11325.
    PaxDbiP11325.
    PeptideAtlasiP11325.

    Expressioni

    Gene expression databases

    GenevestigatoriP11325.

    Interactioni

    Protein-protein interaction databases

    BioGridi31641. 34 interactions.
    IntActiP11325. 1 interaction.
    MINTiMINT-2785917.
    STRINGi4932.YLR382C.

    Structurei

    3D structure databases

    ProteinModelPortaliP11325.
    SMRiP11325. Positions 25-883.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi56 – 6611"HIGH" regionAdd
    BLAST
    Motifi646 – 6505"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0495.
    GeneTreeiENSGT00390000015114.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiHELEVYT.
    OrthoDBiEOG7Q8CWM.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11325-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY    50
    ILCQFPYPSG ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA 100
    ENAAIERSIN PAIWTRDNIA KMKQQMQSML ANFDWDREIT TCDPEYYKFT 150
    QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL ANEQVDAQGR SWRSGAIVEK 200
    KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG ESVGAELVFK 250
    VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK 300
    SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC 350
    PGHDNRDFEF WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS 400
    TDGVLTKECG EHSGVLTVVA RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ 450
    RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELEGLDTKGN PLSTIDEFVN 500
    VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASKNMP 550
    VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV 600
    QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS 650
    KYNGADPNEC ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV 700
    LHLTKNILSL EKDLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV 750
    NLSLNTVISD YMKLTNILES ALKKGEVRNE MIVQNLQKLV TVIYPAVPSI 800
    SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKN 850
    FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK 894
    Length:894
    Mass (Da):101,920
    Last modified:July 1, 1989 - v1
    Checksum:i3862CEFFFB10B262
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03495 Genomic DNA. Translation: AAA34805.1.
    X05143 Genomic DNA. Translation: CAA28791.1.
    U19104 Genomic DNA. Translation: AAB67277.1.
    BK006945 Genomic DNA. Translation: DAA09683.1.
    PIRiA28521. SYBYLM.
    RefSeqiNP_013486.3. NM_001182271.3.

    Genome annotation databases

    EnsemblFungiiYLR382C; YLR382C; YLR382C.
    GeneIDi851098.
    KEGGisce:YLR382C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03495 Genomic DNA. Translation: AAA34805.1 .
    X05143 Genomic DNA. Translation: CAA28791.1 .
    U19104 Genomic DNA. Translation: AAB67277.1 .
    BK006945 Genomic DNA. Translation: DAA09683.1 .
    PIRi A28521. SYBYLM.
    RefSeqi NP_013486.3. NM_001182271.3.

    3D structure databases

    ProteinModelPortali P11325.
    SMRi P11325. Positions 25-883.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31641. 34 interactions.
    IntActi P11325. 1 interaction.
    MINTi MINT-2785917.
    STRINGi 4932.YLR382C.

    Proteomic databases

    MaxQBi P11325.
    PaxDbi P11325.
    PeptideAtlasi P11325.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR382C ; YLR382C ; YLR382C .
    GeneIDi 851098.
    KEGGi sce:YLR382C.

    Organism-specific databases

    CYGDi YLR382c.
    SGDi S000004374. NAM2.

    Phylogenomic databases

    eggNOGi COG0495.
    GeneTreei ENSGT00390000015114.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi HELEVYT.
    OrthoDBi EOG7Q8CWM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32448-MONOMER.

    Miscellaneous databases

    NextBioi 967782.
    PROi P11325.

    Gene expression databases

    Genevestigatori P11325.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli."
      Tzagoloff A., Akai A., Kurkulos M., Repetto B.
      J. Biol. Chem. 263:850-856(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene."
      Labouesse M., Herbert C.J., Dujardin G., Slonimski P.P.
      EMBO J. 6:713-721(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AB1-4A/8/55.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase."
      Zagorski W., Castaing B., Herbert C.J., Labouesse M., Martin R., Slonimski P.P.
      J. Biol. Chem. 266:2537-2541(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYLM_YEAST
    AccessioniPrimary (citable) accession number: P11325
    Secondary accession number(s): D6VZ17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    PubMed:3034607 authors identified this protein as the gene product of the NAM2 gene, which is capable of compensating for mutations in mRNA maturase encoded by the fourth intron of the mitochondrial cytochrome b gene.
    Present with 2120 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3