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P11325

- SYLM_YEAST

UniProt

P11325 - SYLM_YEAST

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Protein

Leucine--tRNA ligase, mitochondrial

Gene
NAM2, MSL1, YLR382C, L3502.6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei649 – 6491ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: SGD
  4. mRNA binding Source: SGD

GO - Biological processi

  1. Group I intron splicing Source: SGD
  2. leucyl-tRNA aminoacylation Source: SGD
  3. mitochondrial translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32448-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:NAM2
Synonyms:MSL1
Ordered Locus Names:YLR382C
ORF Names:L3502.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR382c.
SGDiS000004374. NAM2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 99Mitochondrion
Chaini10 – 894885Leucine--tRNA ligase, mitochondrialPRO_0000035810Add
BLAST

Proteomic databases

MaxQBiP11325.
PaxDbiP11325.
PeptideAtlasiP11325.

Expressioni

Gene expression databases

GenevestigatoriP11325.

Interactioni

Protein-protein interaction databases

BioGridi31641. 34 interactions.
IntActiP11325. 1 interaction.
MINTiMINT-2785917.
STRINGi4932.YLR382C.

Structurei

3D structure databases

ProteinModelPortaliP11325.
SMRiP11325. Positions 25-883.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 6611"HIGH" regionAdd
BLAST
Motifi646 – 6505"KMSKS" region

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000015114.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHELEVYT.
OrthoDBiEOG7Q8CWM.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11325-1 [UniParc]FASTAAdd to Basket

« Hide

MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY    50
ILCQFPYPSG ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA 100
ENAAIERSIN PAIWTRDNIA KMKQQMQSML ANFDWDREIT TCDPEYYKFT 150
QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL ANEQVDAQGR SWRSGAIVEK 200
KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG ESVGAELVFK 250
VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK 300
SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC 350
PGHDNRDFEF WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS 400
TDGVLTKECG EHSGVLTVVA RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ 450
RYWGTPIPII HCDNCGPVPV PESDLPVKLP ELEGLDTKGN PLSTIDEFVN 500
VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF DREIASKNMP 550
VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV 600
QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS 650
KYNGADPNEC ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV 700
LHLTKNILSL EKDLAISKDY KTPTDLNDAE VKFHNDFQRF LKSITESFEV 750
NLSLNTVISD YMKLTNILES ALKKGEVRNE MIVQNLQKLV TVIYPAVPSI 800
SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR VKFMYTADKN 850
FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK 894
Length:894
Mass (Da):101,920
Last modified:July 1, 1989 - v1
Checksum:i3862CEFFFB10B262
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03495 Genomic DNA. Translation: AAA34805.1.
X05143 Genomic DNA. Translation: CAA28791.1.
U19104 Genomic DNA. Translation: AAB67277.1.
BK006945 Genomic DNA. Translation: DAA09683.1.
PIRiA28521. SYBYLM.
RefSeqiNP_013486.3. NM_001182271.3.

Genome annotation databases

EnsemblFungiiYLR382C; YLR382C; YLR382C.
GeneIDi851098.
KEGGisce:YLR382C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03495 Genomic DNA. Translation: AAA34805.1 .
X05143 Genomic DNA. Translation: CAA28791.1 .
U19104 Genomic DNA. Translation: AAB67277.1 .
BK006945 Genomic DNA. Translation: DAA09683.1 .
PIRi A28521. SYBYLM.
RefSeqi NP_013486.3. NM_001182271.3.

3D structure databases

ProteinModelPortali P11325.
SMRi P11325. Positions 25-883.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31641. 34 interactions.
IntActi P11325. 1 interaction.
MINTi MINT-2785917.
STRINGi 4932.YLR382C.

Proteomic databases

MaxQBi P11325.
PaxDbi P11325.
PeptideAtlasi P11325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR382C ; YLR382C ; YLR382C .
GeneIDi 851098.
KEGGi sce:YLR382C.

Organism-specific databases

CYGDi YLR382c.
SGDi S000004374. NAM2.

Phylogenomic databases

eggNOGi COG0495.
GeneTreei ENSGT00390000015114.
HOGENOMi HOG000200747.
KOi K01869.
OMAi HELEVYT.
OrthoDBi EOG7Q8CWM.

Enzyme and pathway databases

BioCyci YEAST:G3O-32448-MONOMER.

Miscellaneous databases

NextBioi 967782.
PROi P11325.

Gene expression databases

Genevestigatori P11325.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli."
    Tzagoloff A., Akai A., Kurkulos M., Repetto B.
    J. Biol. Chem. 263:850-856(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene."
    Labouesse M., Herbert C.J., Dujardin G., Slonimski P.P.
    EMBO J. 6:713-721(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AB1-4A/8/55.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase."
    Zagorski W., Castaing B., Herbert C.J., Labouesse M., Martin R., Slonimski P.P.
    J. Biol. Chem. 266:2537-2541(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYLM_YEAST
AccessioniPrimary (citable) accession number: P11325
Secondary accession number(s): D6VZ17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

1 Publication authors identified this protein as the gene product of the NAM2 gene, which is capable of compensating for mutations in mRNA maturase encoded by the fourth intron of the mitochondrial cytochrome b gene.
Present with 2120 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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