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P11325 (SYLM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase, mitochondrial

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:NAM2
Synonyms:MSL1
Ordered Locus Names:YLR382C
ORF Names:L3502.6
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length894 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Mitochondrion matrix.

Miscellaneous

Ref.2 authors identified this protein as the gene product of the NAM2 gene, which is capable of compensating for mutations in mRNA maturase encoded by the fourth intron of the mitochondrial cytochrome b gene.

Present with 2120 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 99Mitochondrion
Chain10 – 894885Leucine--tRNA ligase, mitochondrial
PRO_0000035810

Regions

Motif56 – 6611"HIGH" region
Motif646 – 6505"KMSKS" region

Sites

Binding site6491ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P11325 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 3862CEFFFB10B262

FASTA894101,920
        10         20         30         40         50         60 
MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY ILCQFPYPSG 

        70         80         90        100        110        120 
ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA ENAAIERSIN PAIWTRDNIA 

       130        140        150        160        170        180 
KMKQQMQSML ANFDWDREIT TCDPEYYKFT QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL 

       190        200        210        220        230        240 
ANEQVDAQGR SWRSGAIVEK KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG 

       250        260        270        280        290        300 
ESVGAELVFK VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK 

       310        320        330        340        350        360 
SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC PGHDNRDFEF 

       370        380        390        400        410        420 
WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS TDGVLTKECG EHSGVLTVVA 

       430        440        450        460        470        480 
RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ RYWGTPIPII HCDNCGPVPV PESDLPVKLP 

       490        500        510        520        530        540 
ELEGLDTKGN PLSTIDEFVN VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF 

       550        560        570        580        590        600 
DREIASKNMP VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV 

       610        620        630        640        650        660 
QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS KYNGADPNEC 

       670        680        690        700        710        720 
ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV LHLTKNILSL EKDLAISKDY 

       730        740        750        760        770        780 
KTPTDLNDAE VKFHNDFQRF LKSITESFEV NLSLNTVISD YMKLTNILES ALKKGEVRNE 

       790        800        810        820        830        840 
MIVQNLQKLV TVIYPAVPSI SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR 

       850        860        870        880        890 
VKFMYTADKN FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK 

« Hide

References

« Hide 'large scale' references
[1]"Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli."
Tzagoloff A., Akai A., Kurkulos M., Repetto B.
J. Biol. Chem. 263:850-856(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene."
Labouesse M., Herbert C.J., Dujardin G., Slonimski P.P.
EMBO J. 6:713-721(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AB1-4A/8/55.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase."
Zagorski W., Castaing B., Herbert C.J., Labouesse M., Martin R., Slonimski P.P.
J. Biol. Chem. 266:2537-2541(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03495 Genomic DNA. Translation: AAA34805.1.
X05143 Genomic DNA. Translation: CAA28791.1.
U19104 Genomic DNA. Translation: AAB67277.1.
BK006945 Genomic DNA. Translation: DAA09683.1.
PIRSYBYLM. A28521.
RefSeqNP_013486.3. NM_001182271.3.

3D structure databases

ProteinModelPortalP11325.
SMRP11325. Positions 25-883.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31641. 34 interactions.
IntActP11325. 1 interaction.
MINTMINT-2785917.
STRING4932.YLR382C.

Proteomic databases

MaxQBP11325.
PaxDbP11325.
PeptideAtlasP11325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR382C; YLR382C; YLR382C.
GeneID851098.
KEGGsce:YLR382C.

Organism-specific databases

CYGDYLR382c.
SGDS000004374. NAM2.

Phylogenomic databases

eggNOGCOG0495.
GeneTreeENSGT00390000015114.
HOGENOMHOG000200747.
KOK01869.
OMAHELEVYT.
OrthoDBEOG7Q8CWM.

Enzyme and pathway databases

BioCycYEAST:G3O-32448-MONOMER.

Gene expression databases

GenevestigatorP11325.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967782.
PROP11325.

Entry information

Entry nameSYLM_YEAST
AccessionPrimary (citable) accession number: P11325
Secondary accession number(s): D6VZ17
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries