Reviewed, UniProtKB/Swiss-Prot P11308 (ERG_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcriptional regulator ERG
Alternative name(s):
    Transforming protein ERG

Gene names

Name:

ERG

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
Subunit structure	Interacts with SETDB1 By similarity.
Subcellular location	Nucleus.
Involvement in disease	A chromosomal aberration involving ERG is a cause of Erwing sarcoma [MIM:133450]. Translocation t(21;22)(q22;q12) with ERW. A chromosomal aberration involving ERG is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement
Disease	Proto-oncogene
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell proliferation Ref.2 Traceable author statement. Source: ProtInc multicellular organismal development Traceable author statement. Source: ProtInc protein amino acid phosphorylation Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Traceable author statement. Source: ProtInc transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro signal transducer activity Traceable author statement. Source: ProtInc transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SETDB1	Q15047	1	EBI-79704,EBI-79691

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform ERG-3 (identifier: P11308-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform ERG-2 (identifier: P11308-1)

The sequence of this isoform differs from the canonical sequence as follows:
     232-255: Missing.

Isoform ERG-1 (identifier: P11308-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.

Isoform 5 (identifier: P11308-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MIQTVPDPAAH → MAST

Isoform 7 (identifier: P11308-5)

The sequence of this isoform differs from the canonical sequence as follows:
     315-317: SGQ → WTQ
     318-486: Missing.

Isoform 8 (identifier: P11308-6)

The sequence of this isoform differs from the canonical sequence as follows:
     256-325: DLPYEPPRRS...GQIQLWQFLL → GTKTPLCDLF...KTTLKELRAD
     326-486: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 486

486

Transcriptional regulator ERG

PRO_0000204103

Regions

Domain

120 – 206

87

PNT

DNA binding

318 – 398

81

ETS

Amino acid modifications

Modified residue

93

1

Phosphoserine Ref.9

Natural variations

Alternative sequence

1 – 99

99

Missing in isoform ERG-1.

VSP_001469

Alternative sequence

1 – 11

11

MIQTVPDPAAH → MAST in isoform 5.

VSP_026584

Alternative sequence

232 – 255

24

Missing in isoform ERG-2.

VSP_026579

Alternative sequence

256 – 325

70

DLPYE…WQFLL → GTKTPLCDLFIERHPRCPAE IRALSHVIQRELIPELKPVP DSLILPLLIWRLNPLKPFHS KTTLKELRAD in isoform 8.

VSP_026580

Alternative sequence

315 – 317

3

SGQ → WTQ in isoform 7.

VSP_026582

Alternative sequence

318 – 486

169

Missing in isoform 7.

VSP_026583

Alternative sequence

326 – 486

161

Missing in isoform 8.

VSP_026581

Secondary structure

1

.

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.

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486

Helix Strand Turn

Details...

Helix

116 – 119

4

Turn

127 – 130

4

Beta strand

131 – 134

4

Helix

143 – 157

15

Helix

164 – 166

3

Helix

172 – 175

4

Helix

180 – 183

4

Turn

184 – 186

3

Helix

189 – 202

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform ERG-3 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: A0B548DFAE25029E	FASTA	486	54,608
	10 20 30 40 50 60 MIQTVPDPAA HIKEALSVVS EDQSLFECAY GTPHLAKTEM TASSSSDYGQ TSKMSPRVPQ 70 80 90 100 110 120 QDWLSQPPAR VTIKMECNPS QVNGSRNSPD ECSVAKGGKM VGSPDTVGMN YGSYMEEKHM 130 140 150 160 170 180 PPPNMTTNER RVIVPADPTL WSTDHVRQWL EWAVKEYGLP DVNILLFQNI DGKELCKMTK 190 200 210 220 230 240 DDFQRLTPSY NADILLSHLH YLRETPLPHL TSDDVDKALQ NSPRLMHARN TGGAAFIFPN 250 260 270 280 290 300 TSVYPEATQR ITTRPDLPYE PPRRSAWTGH GHPTPQSKAA QPSPSTVPKT EDQRPQLDPY 310 320 330 340 350 360 QILGPTSSRL ANPGSGQIQL WQFLLELLSD SSNSSCITWE GTNGEFKMTD PDEVARRWGE 370 380 390 400 410 420 RKSKPNMNYD KLSRALRYYY DKNIMTKVHG KRYAYKFDFH GIAQALQPHP PESSLYKYPS 430 440 450 460 470 480 DLPYMGSYHA HPQKMNFVAP HPPALPVTSS SFFAAPNPYW NSPTGGIYPN TRLPTSHMPS HLGTYY
	Isoform ERG-2. Checksum: B29F14B0F5C2C697	FASTA	462	52,031
	10 20 30 40 50 60 MIQTVPDPAA HIKEALSVVS EDQSLFECAY GTPHLAKTEM TASSSSDYGQ TSKMSPRVPQ 70 80 90 100 110 120 QDWLSQPPAR VTIKMECNPS QVNGSRNSPD ECSVAKGGKM VGSPDTVGMN YGSYMEEKHM 130 140 150 160 170 180 PPPNMTTNER RVIVPADPTL WSTDHVRQWL EWAVKEYGLP DVNILLFQNI DGKELCKMTK 190 200 210 220 230 240 DDFQRLTPSY NADILLSHLH YLRETPLPHL TSDDVDKALQ NSPRLMHARN TDLPYEPPRR 250 260 270 280 290 300 SAWTGHGHPT PQSKAAQPSP STVPKTEDQR PQLDPYQILG PTSSRLANPG SGQIQLWQFL 310 320 330 340 350 360 LELLSDSSNS SCITWEGTNG EFKMTDPDEV ARRWGERKSK PNMNYDKLSR ALRYYYDKNI 370 380 390 400 410 420 MTKVHGKRYA YKFDFHGIAQ ALQPHPPESS LYKYPSDLPY MGSYHAHPQK MNFVAPHPPA 430 440 450 460 LPVTSSSFFA APNPYWNSPT GGIYPNTRLP TSHMPSHLGT YY
	Isoform ERG-1. Checksum: 6C19CE3D70C769EA	FASTA	387	43,974
	10 20 30 40 50 60 MVGSPDTVGM NYGSYMEEKH MPPPNMTTNE RRVIVPADPT LWSTDHVRQW LEWAVKEYGL 70 80 90 100 110 120 PDVNILLFQN IDGKELCKMT KDDFQRLTPS YNADILLSHL HYLRETPLPH LTSDDVDKAL 130 140 150 160 170 180 QNSPRLMHAR NTGGAAFIFP NTSVYPEATQ RITTRPDLPY EPPRRSAWTG HGHPTPQSKA 190 200 210 220 230 240 AQPSPSTVPK TEDQRPQLDP YQILGPTSSR LANPGSGQIQ LWQFLLELLS DSSNSSCITW 250 260 270 280 290 300 EGTNGEFKMT DPDEVARRWG ERKSKPNMNY DKLSRALRYY YDKNIMTKVH GKRYAYKFDF 310 320 330 340 350 360 HGIAQALQPH PPESSLYKYP SDLPYMGSYH AHPQKMNFVA PHPPALPVTS SSFFAAPNPY 370 380 WNSPTGGIYP NTRLPTSHMP SHLGTYY
	Isoform 5. Checksum: A5069C393E409483	FASTA	479	53,838
	10 20 30 40 50 60 MASTIKEALS VVSEDQSLFE CAYGTPHLAK TEMTASSSSD YGQTSKMSPR VPQQDWLSQP 70 80 90 100 110 120 PARVTIKMEC NPSQVNGSRN SPDECSVAKG GKMVGSPDTV GMNYGSYMEE KHMPPPNMTT 130 140 150 160 170 180 NERRVIVPAD PTLWSTDHVR QWLEWAVKEY GLPDVNILLF QNIDGKELCK MTKDDFQRLT 190 200 210 220 230 240 PSYNADILLS HLHYLRETPL PHLTSDDVDK ALQNSPRLMH ARNTGGAAFI FPNTSVYPEA 250 260 270 280 290 300 TQRITTRPDL PYEPPRRSAW TGHGHPTPQS KAAQPSPSTV PKTEDQRPQL DPYQILGPTS 310 320 330 340 350 360 SRLANPGSGQ IQLWQFLLEL LSDSSNSSCI TWEGTNGEFK MTDPDEVARR WGERKSKPNM 370 380 390 400 410 420 NYDKLSRALR YYYDKNIMTK VHGKRYAYKF DFHGIAQALQ PHPPESSLYK YPSDLPYMGS 430 440 450 460 470 YHAHPQKMNF VAPHPPALPV TSSSFFAAPN PYWNSPTGGI YPNTRLPTSH MPSHLGTYY
	Isoform 7. Checksum: A30FAAD4E5E119CC	FASTA	317	35,286
	10 20 30 40 50 60 MIQTVPDPAA HIKEALSVVS EDQSLFECAY GTPHLAKTEM TASSSSDYGQ TSKMSPRVPQ 70 80 90 100 110 120 QDWLSQPPAR VTIKMECNPS QVNGSRNSPD ECSVAKGGKM VGSPDTVGMN YGSYMEEKHM 130 140 150 160 170 180 PPPNMTTNER RVIVPADPTL WSTDHVRQWL EWAVKEYGLP DVNILLFQNI DGKELCKMTK 190 200 210 220 230 240 DDFQRLTPSY NADILLSHLH YLRETPLPHL TSDDVDKALQ NSPRLMHARN TGGAAFIFPN 250 260 270 280 290 300 TSVYPEATQR ITTRPDLPYE PPRRSAWTGH GHPTPQSKAA QPSPSTVPKT EDQRPQLDPY 310 QILGPTSSRL ANPGWTQ
	Isoform 8. Checksum: CF149350960CCE05	FASTA	325	36,541
	10 20 30 40 50 60 MIQTVPDPAA HIKEALSVVS EDQSLFECAY GTPHLAKTEM TASSSSDYGQ TSKMSPRVPQ 70 80 90 100 110 120 QDWLSQPPAR VTIKMECNPS QVNGSRNSPD ECSVAKGGKM VGSPDTVGMN YGSYMEEKHM 130 140 150 160 170 180 PPPNMTTNER RVIVPADPTL WSTDHVRQWL EWAVKEYGLP DVNILLFQNI DGKELCKMTK 190 200 210 220 230 240 DDFQRLTPSY NADILLSHLH YLRETPLPHL TSDDVDKALQ NSPRLMHARN TGGAAFIFPN 250 260 270 280 290 300 TSVYPEATQR ITTRPGTKTP LCDLFIERHP RCPAEIRALS HVIQRELIPE LKPVPDSLIL 310 320 PLLIWRLNPL KPFHSKTTLK ELRAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation." Rao V.N., Papas T.S., Shyam E., Reddy P. Science 237:635-639(1987) [PubMed: 3299708] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM ERG-2).
[2]	"The erg gene: a human gene related to the ets oncogene." Reddy E.S.P., Rao V.N., Papas T.S. Proc. Natl. Acad. Sci. U.S.A. 84:6131-6135(1987) [PubMed: 3476934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ERG-1).
[3]	"Detailed mapping of the ERG-ETS2 interval of human chromosome 21 and comparison with the region of conserved synteny on mouse chromosome 16." Owczarek C.M., Portbury K.J., Hardy M.P., O'Leary D.A., Kudoh J., Shibuya K., Shimizu N., Kola I., Hertzog P.J. Gene 324:65-77(2004) [PubMed: 14693372] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 7).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND ERG-1). Tissue: Thymus.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Testis.
[6]	"Differentially spliced erg-3 product functions as a transcriptional activator." Prasad D.D., Rao V.N., Lee L., Reddy E.S. Oncogene 9:669-673(1994) [PubMed: 8290279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 230-259 (ISOFORM ERG-3).
[7]	"ERG gene is translocated in an Ewing's sarcoma cell line." Dunn T., Praissman L., Hagag N., Viola M.V. Cancer Genet. Cytogenet. 76:19-22(1994) [PubMed: 8076344] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION.
[8]	"An RNA-binding protein gene, TLS/FUS, is fused to ERG in human myeloid leukemia with t(16;21) chromosomal translocation." Ichikawa H., Shimizu K., Hayashi Y., Ohki M. Cancer Res. 54:2865-2868(1994) [PubMed: 8187069] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION.
[9]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Diversity in structure and function of the Ets family PNT domains." Mackereth C.D., Scharpf M., Gentile L.N., MacIntosh S.E., Slupsky C.M., McIntosh L.P. J. Mol. Biol. 342:1249-1264(2004) [PubMed: 15351649] [Abstract] Cited for: STRUCTURE BY NMR OF 112-208.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

M17254 Genomic RNA. Translation: AAA52398.1.
M21535 mRNA. Translation: AAA35811.1.
AY204741 mRNA. Translation: AAP41719.1.
AY204742 mRNA. Translation: AAP41720.1.
AK300395 mRNA. Translation: BAG62127.1.
AK303518 mRNA. Translation: BAG64546.1.
BC040168 mRNA. Translation: AAH40168.1.
S68130 mRNA. Translation: AAB29724.1.

IPI

IPI00005012.
IPI00217544.
IPI00220990.
IPI00549287.
IPI00788647.
IPI00853428.

PIR

TVHUEG. A94294.

RefSeq

NP_001129626.1.
NP_001129627.1.
NP_891548.1.

UniGene

Hs.473819

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SXE	NMR	-	A	112-208	[»]

SMR

P11308. Positions 289-386.

ModBase

Search...

Protein-protein interaction databases

IntAct

P11308. 1 interaction.

PTM databases

PhosphoSite

P11308.

Proteomic databases

PRIDE

P11308.

Genome annotation databases

Ensembl

ENSG00000157554. Homo sapiens. [Contig view]

GeneID

2078.

KEGG

hsa:2078.

Organism-specific databases

GeneCards

GC21M038675.

H-InvDB

HIX0027780.

HGNC

HGNC:3446. ERG.

HPA

CAB018377.

MIM

133450. gene+phenotype.
165080. gene.

Orphanet

1957. Esthesioneuroblastoma.
319. Ewing sarcoma.

PharmGKB

PA27858.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P11308.

Gene expression databases

ArrayExpress

P11308.

Bgee

P11308.

CleanEx

HS_ERG.

GermOnline

ENSG00000157554. Homo sapiens.

Family and domain databases

InterPro

IPR000418. Ets.
IPR003118. SAM_PNT.
IPR011991. Wing_hlx_DNA_bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]

PRINTS

PR00454. ETSDOMAIN.

SMART

SM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]

PROSITE

PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

8447.

SOURCE

Search...

Entry information

Entry name

ERG_HUMAN

Accession

Primary (citable) accession number: P11308
Secondary accession number(s): B4DTW5 , B4E0T4, Q16113, Q6XXX4, Q6XXX5, Q8IXK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)