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Protein

Gag polyprotein

Gene

gag

Organism
Mouse mammary tumor virus (strain C3H) (MMTV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Matrix protein p10: Matrix protein.Curated
Nucleocapsid protein p14: Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.Curated
Capsid protein p27: Capsid protein.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri525 – 542CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri552 – 569CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Gene namesi
Name:gag
OrganismiMouse mammary tumor virus (strain C3H) (MMTV)
Taxonomic identifieri11759 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeBetaretrovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000006540 Componenti: Genome

Subcellular locationi

Matrix protein p10 :
  • Virion By similarity
Capsid protein p27 :
  • Virion By similarity
Nucleocapsid protein p14 :
  • Virion By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by host1 Publication
ChainiPRO_00004424662 – 591Gag polyproteinAdd BLAST590
ChainiPRO_00000409272 – 99Matrix protein p10Add BLAST98
ChainiPRO_0000040928100 – 195Phosphorylated protein pp21Add BLAST96
ChainiPRO_0000040929196 – 228Protein p3Add BLAST33
ChainiPRO_0000040930229 – 254Protein p8Add BLAST26
ChainiPRO_0000040931255 – 269Protein nAdd BLAST15
ChainiPRO_0000040932270 – 496Capsid protein p27Add BLAST227
ChainiPRO_0000040933497 – 591Nucleocapsid protein p14Add BLAST95

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by host1 Publication1

Post-translational modificationi

Gag polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.1 Publication
Gag polyprotein: Specific enzymatic cleavages in vivo yield mature proteins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei99 – 100Cleavage; by viral protease1 Publication2
Sitei195 – 196Cleavage; by viral protease1 Publication2
Sitei228 – 229Cleavage; by viral protease1 Publication2
Sitei254 – 255Cleavage; by viral proteaseBy similarity2
Sitei269 – 270Cleavage; by viral protease1 Publication2
Sitei496 – 497Cleavage; by viral protease1 Publication2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

PTM databases

iPTMnetiP11284

Interactioni

Subunit structurei

Matrix protein p10: Homodimer; when myristoylated.By similarity

Protein-protein interaction databases

ELMiP11284

Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni528 – 530Combined sources3
Beta strandi533 – 535Combined sources3
Turni537 – 541Combined sources5
Turni555 – 557Combined sources3
Beta strandi559 – 562Combined sources4
Turni564 – 566Combined sources3
Beta strandi570 – 572Combined sources3
Helixi573 – 575Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DSQNMR-A523-543[»]
1DSVNMR-A550-580[»]
ProteinModelPortaliP11284
SMRiP11284
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11284

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi305 – 308PTAP/PSAP motifCurated4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 281Poly-Asp4

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Gag-p27 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri525 – 542CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri552 – 569CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

OrthoDBiVOG09000135

Family and domain databases

Gene3Di1.10.1200.30, 1 hit
1.10.150.490, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR003322 B_retro_matrix
IPR038124 B_retro_matrix_sf
IPR000721 Gag_p24
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF02337 Gag_p10, 1 hit
PF00607 Gag_p24, 1 hit
PF00098 zf-CCHC, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004265 B_retro_matrix_N, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 2 hits
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Gag polyprotein (identifier: P11284-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVSGSKGQK LFVSVLQRLL SERGLHVKES SAIEFYQFLI KVSPWFPEEG
60 70 80 90 100
GLNLQDWKRV GREMKKYAAE HGTDSIPKQA YPIWLQLREI LTEQSDLVLL
110 120 130 140 150
SAEAKSVTEE ELEEGLTGLL SASSQEKTYG TRGTAYAEID TEVDKLSEHI
160 170 180 190 200
YDEPYEEKEK ADKNEEKDHV RKVKKIVQRK ENSEHKRKEK DQKAFLATDW
210 220 230 240 250
NNDDLSPEDW DDLEEQAAHY HDDDELILPV KRKVDKKKPL ALRRKPLPPV
260 270 280 290 300
GFAGAMAEAR EKGDLTFTFP VVFMGESDDD DTPVWEPLPL KTLKELQSAV
310 320 330 340 350
RTMGPSAPYT LQVVDMVASQ WLTPSDWHQT ARATLSPGDY VLWRTEYEEK
360 370 380 390 400
SKETVQKTAG KRKGKVSLDM LLGTGQFLSP SSQIKLSKDV LKDVTTNAVL
410 420 430 440 450
AWRAIPPPGV KKTVLAGLKQ GNEESYETFI SRLEEAVYRV MPRGEGSDIL
460 470 480 490 500
IKQLAWENAN SLCQDLIRPM RKTGTMQDYI RACLDASPAV VQGMAYAAAM
510 520 530 540 550
RGQKYSTFVK QTYGGGKGGQ GSKGPVCFSC GKTGHIKRDC KEEKGSKRAP
560 570 580 590
PGLCPRCKKG YHWKSECKSK FDKDGNPLPP LETNAENSKN L
Note: Produced by conventional translation.1 Publication
Length:591
Mass (Da):66,351
Last modified:January 23, 2007 - v3
Checksum:i8F9E3C05D6F7B9AC
GO
Isoform Gag-Pro polyprotein (identifier: Q9IZT2-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9IZT2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting between gag-pro.1 Publication
Length:860
Mass (Da):95,593
GO
Isoform Gag-Pro-Pol polyprotein (identifier: P11283-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P11283.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshiftings between gag-pro and pro-pol.1 Publication
Length:1,755
Mass (Da):197,329
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106S → T AA sequence (PubMed:2542570).Curated1
Sequence conflicti203D → N AA sequence (PubMed:2542570).Curated1
Sequence conflicti440V → M in AAF31472 (PubMed:10982330).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti523K → E. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228552 Genomic DNA Translation: AAF31472.1
M16766 Genomic RNA Translation: AAA66623.1
PIRiA29029

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiGAG_MMTVC
AccessioniPrimary (citable) accession number: P11284
Secondary accession number(s): Q9IZT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 121 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health