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Protein

Gag polyprotein

Gene

gag

Organism
Mouse mammary tumor virus (strain C3H) (MMTV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Matrix protein p10: Matrix protein.
Nucleocapsid protein p14: Nucleocapsid protein. Binds to single-stranded DNA.
Capsid protein p27: capsid protein.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri525 – 542CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri552 – 569CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

DNA-binding, Metal-binding, Nucleotide-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Gene namesi
Name:gag
OrganismiMouse mammary tumor virus (strain C3H) (MMTV)
Taxonomic identifieri11759 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeBetaretrovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000006540 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by host1 Publication
ChainiPRO_00000409272 – 99Matrix protein p10Add BLAST98
ChainiPRO_0000040928100 – 195Phosphorylated protein pp21Add BLAST96
ChainiPRO_0000040929196 – 228Protein p3Add BLAST33
ChainiPRO_0000040930229 – 252Protein p8Sequence analysisAdd BLAST24
ChainiPRO_0000040931253 – 269Protein nAdd BLAST17
ChainiPRO_0000040932270 – 496Capsid protein p27Add BLAST227
ChainiPRO_0000040933497 – 591Nucleocapsid protein p14Add BLAST95

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by host1 Publication1

Post-translational modificationi

p10 is myristoylated.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei99 – 100Cleavage; by viral proteaseBy similarity2
Sitei195 – 196Cleavage; by viral proteaseBy similarity2
Sitei228 – 229Cleavage; by viral proteaseBy similarity2
Sitei252 – 253Cleavage; by viral proteaseBy similarity2
Sitei269 – 270Cleavage; by viral proteaseBy similarity2
Sitei496 – 497Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni528 – 530Combined sources3
Beta strandi533 – 535Combined sources3
Turni537 – 541Combined sources5
Turni555 – 557Combined sources3
Beta strandi559 – 562Combined sources4
Turni564 – 566Combined sources3
Beta strandi570 – 572Combined sources3
Helixi573 – 575Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DSQNMR-A523-543[»]
1DSVNMR-A550-580[»]
ProteinModelPortaliP11284.
SMRiP11284.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11284.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi305 – 308PTAP/PSAP motifSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 281Poly-Asp4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p27 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 (Potential).Curated

Sequence similaritiesi

Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri525 – 542CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri552 – 569CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR003322. B_retro_matrix.
IPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF02337. Gag_p10. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
ProDomiPD004265. B_retro_matrix_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Note: Translation results in the formation of the Gag-Pro. Ribosomal frameshifting at the gag-pro/pol genes boundary produces the Gag-Pro-Pol polyprotein.1 Publication
Isoform Gag polyprotein (identifier: P11284-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVSGSKGQK LFVSVLQRLL SERGLHVKES SAIEFYQFLI KVSPWFPEEG
60 70 80 90 100
GLNLQDWKRV GREMKKYAAE HGTDSIPKQA YPIWLQLREI LTEQSDLVLL
110 120 130 140 150
SAEAKSVTEE ELEEGLTGLL SASSQEKTYG TRGTAYAEID TEVDKLSEHI
160 170 180 190 200
YDEPYEEKEK ADKNEEKDHV RKVKKIVQRK ENSEHKRKEK DQKAFLATDW
210 220 230 240 250
NNDDLSPEDW DDLEEQAAHY HDDDELILPV KRKVDKKKPL ALRRKPLPPV
260 270 280 290 300
GFAGAMAEAR EKGDLTFTFP VVFMGESDDD DTPVWEPLPL KTLKELQSAV
310 320 330 340 350
RTMGPSAPYT LQVVDMVASQ WLTPSDWHQT ARATLSPGDY VLWRTEYEEK
360 370 380 390 400
SKETVQKTAG KRKGKVSLDM LLGTGQFLSP SSQIKLSKDV LKDVTTNAVL
410 420 430 440 450
AWRAIPPPGV KKTVLAGLKQ GNEESYETFI SRLEEAVYRV MPRGEGSDIL
460 470 480 490 500
IKQLAWENAN SLCQDLIRPM RKTGTMQDYI RACLDASPAV VQGMAYAAAM
510 520 530 540 550
RGQKYSTFVK QTYGGGKGGQ GSKGPVCFSC GKTGHIKRDC KEEKGSKRAP
560 570 580 590
PGLCPRCKKG YHWKSECKSK FDKDGNPLPP LETNAENSKN L
Note: Produced by conventional translation.
Length:591
Mass (Da):66,351
Last modified:January 23, 2007 - v3
Checksum:i8F9E3C05D6F7B9AC
GO
Isoform Gag-Pro polyprotein (identifier: Q9IZT2-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9IZT2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting between gag-pro.
Length:860
Mass (Da):95,593
GO
Isoform Gag-Pro-Pol polyprotein (identifier: P11283-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P11283.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting between gag-pro and pro-pol.
Length:1,755
Mass (Da):197,329
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106S → T AA sequence (PubMed:2542570).Curated1
Sequence conflicti203D → N AA sequence (PubMed:2542570).Curated1
Sequence conflicti440V → M in AAF31472 (PubMed:10982330).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti523K → E.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228552 Genomic DNA. Translation: AAF31472.1.
M16766 Genomic RNA. Translation: AAA66623.1.
PIRiA29029.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228552 Genomic DNA. Translation: AAF31472.1.
M16766 Genomic RNA. Translation: AAA66623.1.
PIRiA29029.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DSQNMR-A523-543[»]
1DSVNMR-A550-580[»]
ProteinModelPortaliP11284.
SMRiP11284.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP11284.

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR003322. B_retro_matrix.
IPR000721. Gag_p24.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF02337. Gag_p10. 1 hit.
PF00607. Gag_p24. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
ProDomiPD004265. B_retro_matrix_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAG_MMTVC
AccessioniPrimary (citable) accession number: P11284
Secondary accession number(s): Q9IZT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.