Lysosome-associated membrane glycoprotein 1 precursor

Names and origin

Protein names

Recommended name:
    Lysosome-associated membrane glycoprotein 1
      Short name=LAMP-1
Alternative name(s):
    CD107 antigen-like family member A
    CD_antigen=CD107a

Gene names

Name:

LAMP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Post-translational modification	O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. Ref.10 Ref.11 Ref.12
Sequence similarities	Belongs to the LAMP family.

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Ontologies

Keywords
Cellular component	Cell membrane Endosome Lysosome Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement. Source: ProtInc lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Ref.1 Ref.5

Chain

29 – 417

389

Lysosome-associated membrane glycoprotein 1

PRO_0000017104

Regions

Topological domain

29 – 382

354

Lumenal Potential

Transmembrane

383 – 405

23

Potential

Topological domain

406 – 417

12

Cytoplasmic Potential

Region

29 – 194

166

First lumenal domain

Region

195 – 227

33

Hinge

Region

228 – 382

155

Second lumenal domain

Amino acid modifications

Glycosylation

37

1

N-linked (GlcNAc...)

Glycosylation

45

1

N-linked (GlcNAc...)

Glycosylation

62

1

N-linked (GlcNAc...) (polylactosaminoglycan) Ref.11 Ref.9

Glycosylation

76

1

N-linked (GlcNAc...) Ref.9

Glycosylation

84

1

N-linked (GlcNAc...)

Glycosylation

103

1

N-linked (GlcNAc...) Ref.11 Ref.12

Glycosylation

107

1

N-linked (GlcNAc...)

Glycosylation

121

1

N-linked (GlcNAc...) (polylactosaminoglycan) Ref.9

Glycosylation

130

1

N-linked (GlcNAc...) (polylactosaminoglycan) Ref.9

Glycosylation

165

1

N-linked (GlcNAc...) Ref.9

Glycosylation

181

1

N-linked (GlcNAc...) Ref.9

Glycosylation

197

1

O-linked (GalNAc...); partial Ref.10

Glycosylation

199

1

O-linked (GalNAc...) Ref.10

Glycosylation

200

1

O-linked (GalNAc...) Ref.10

Glycosylation

207

1

O-linked (GalNAc...) Ref.10

Glycosylation

209

1

O-linked (GalNAc...) Ref.10

Glycosylation

211

1

O-linked (GalNAc...) Ref.10

Glycosylation

223

1

N-linked (GlcNAc...) (polylactosaminoglycan) Ref.9

Glycosylation

228

1

N-linked (GlcNAc...) (polylactosaminoglycan) Ref.9

Glycosylation

241

1

N-linked (GlcNAc...)

Glycosylation

249

1

N-linked (GlcNAc...) Ref.12

Glycosylation

261

1

N-linked (GlcNAc...) Ref.9

Glycosylation

293

1

N-linked (GlcNAc...)

Glycosylation

322

1

N-linked (GlcNAc...)

Disulfide bond

Disulfide bond

Disulfide bond

Disulfide bond

Natural variations

Natural variant

309

1

I → T: dbSNP rs9577230.

VAR_046450

Experimental info

Sequence conflict

2

1

A → M in AAA60382. Ref.1

Sequence conflict

5

1

G → R in AAA60382. Ref.1

Sequence conflict

16 – 26

11

LLLLLGLMHCA → PVAAARPHALS in AAA60382. Ref.1

Sequence conflict

33 – 40

8

VKNGNGTA → MARGGRVR in AAA59524. Ref.6

Sequence conflict

262

1

K → T in AAF66141. Ref.7

Sequence conflict

377

1

Missing in AAF66141. Ref.7

Sequence conflict

382

1

M → T in AAA60382. Ref.1

Sequence conflict

382

1

M → T in AAA59524. Ref.6

Sequence conflict

382

1

M → T in AAF66141. Ref.7

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Sequences

Sequence

Length

Mass (Da)

Tools

P11279-1 [UniParc].

Last modified September 23, 2008. Version 3.
Checksum: 3E0A285744DD6588
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FASTA

417

44,882

        10         20         30         40         50         60 
MAAPGSARRP LLLLLLLLLL GLMHCASAAM FMVKNGNGTA CIMANFSAAF SVNYDTKSGP 

        70         80         90        100        110        120 
KNMTFDLPSD ATVVLNRSSC GKENTSDPSL VIAFGRGHTL TLNFTRNATR YSVQLMSFVY 

       130        140        150        160        170        180 
NLSDTHLFPN ASSKEIKTVE SITDIRADID KKYRCVSGTQ VHMNNVTVTL HDATIQAYLS 

       190        200        210        220        230        240 
NSSFSRGETR CEQDRPSPTT APPAPPSPSP SPVPKSPSVD KYNVSGTNGT CLLASMGLQL 

       250        260        270        280        290        300 
NLTYERKDNT TVTRLLNINP NKTSASGSCG AHLVTLELHS EGTTVLLFQF GMNASSSRFF 

       310        320        330        340        350        360 
LQGIQLNTIL PDARDPAFKA ANGSLRALQA TVGNSYKCNA EEHVRVTKAF SVNIFKVWVQ 

       370        380        390        400        410 
AFKVEGGQFG SVEECLLDEN SMLIPIAVGG ALAGLVLIVL IAYLVGRKRS HAGYQTI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences." Fukuda M., Viitala J., Matteson J., Carlsson S.R. J. Biol. Chem. 263:18920-18928(1988) [PubMed: 3198605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-51; 117-131 AND 164-198.
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Pancreas and Testis.
[5]	"Purification and characterization of human lysosomal membrane glycoproteins." Mane S.M., Marzella L., Bainton D.F., Holt V.K., Cha Y., Hildreth J.E.K., August J.T. Arch. Biochem. Biophys. 268:360-378(1989) [PubMed: 2912382] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-59.
[6]	"Molecular cloning of cDNAs encoding lamp A, a human lysosomal membrane glycoprotein with apparent Mr approximately equal to 120,000." Viitala J., Carlsson S.R., Siebert P.D., Fukuda M. Proc. Natl. Acad. Sci. U.S.A. 85:3743-3747(1988) [PubMed: 3131762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-417, PARTIAL PROTEIN SEQUENCE.
[7]	"The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes." Sawada R., Jardine K.A., Fukuda M. J. Biol. Chem. 268:9014-9022(1993) [PubMed: 8517882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-417. Tissue: Placenta.
[8]	"Structure of human lysosomal membrane glycoprotein 1. Assignment of disulfide bonds and visualization of its domain arrangement." Carlsson S.R., Fukuda M. J. Biol. Chem. 264:20526-20531(1989) [PubMed: 2584229] [Abstract] Cited for: DISULFIDE BONDS.
[9]	"The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones." Carlsson S.R., Fukuda M. J. Biol. Chem. 265:20488-20495(1990) [PubMed: 2243102] [Abstract] Cited for: POLYLACTOSAMINOGLYCANS.
[10]	"Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2." Carlsson S.R., Lycksell P.-O., Fukuda M. Arch. Biochem. Biophys. 304:65-73(1993) [PubMed: 8323299] [Abstract] Cited for: GLYCOSYLATION OF HINGE REGION, PROTEIN SEQUENCE OF 191-215.
[11]	"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003) [PubMed: 12754519] [Abstract] Cited for: GLYCOSYLATION AT ASN-62 AND ASN-103.
[12]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103 AND ASN-249, MASS SPECTROMETRY. Tissue: Plasma.
[13]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[14]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-62; ASN-76; ASN-84; ASN-103; ASN-121; ASN-130; ASN-249 AND ASN-293, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	J04182 mRNA. Translation: AAA60382.1. AL136221 Genomic DNA. Translation: CAI13797.1. CH471085 Genomic DNA. Translation: EAX09202.1. BC006345 mRNA. Translation: AAH06345.2. BC007845 mRNA. Translation: AAH07845.2. BC021288 mRNA. Translation: AAH21288.1. BC093044 mRNA. Translation: AAH93044.1. J03263 mRNA. Translation: AAA59524.1. L08582 L08581 Genomic DNA. Translation: AAF66141.1.
IPI	IPI00884105.
PIR	A31959.
RefSeq	NP_005552.3.
UniGene	Hs.494419
3D structure databases
ModBase	Search...
PTM databases
GlycoSuiteDB	P11279.
PhosphoSite	P11279.
Proteomic databases
PRIDE	P11279.
Genome annotation databases
Ensembl	ENSG00000185896. Homo sapiens. [Contig view]
GeneID	3916.
KEGG	hsa:3916.
Organism-specific databases
GeneCards	GC13P112999.
H-InvDB	HIX0011475.
HGNC	HGNC:6499. LAMP1.
HPA	HPA014750.
MIM	153330. gene.
PharmGKB	PA30283.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P11279.
OMA	P11279. ENNMLIP.
Gene expression databases
ArrayExpress	P11279.
Bgee	P11279.
CleanEx	HS_LAMP1.
GermOnline	ENSG00000185896. Homo sapiens.
Family and domain databases
InterPro	IPR002000. Lamp. IPR018133. LAMP/LAMP-like_membrane. IPR018134. LAMP_CS. [Graphical view]
Pfam	PF01299. Lamp. 1 hit. [Graphical view]
PRINTS	PR00336. LYSASSOCTDMP.
PROSITE	PS00310. LAMP_1. 2 hits. PS00311. LAMP_2. 1 hit. PS51407. LAMP_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

LAMP1_HUMAN

Accession

Primary (citable) accession number: P11279
Secondary accession number(s): Q8WU33

Q9NP13

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	September 23, 2008
Last modified:	June 16, 2009
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents