Reviewed, UniProtKB/Swiss-Prot P11278 (PELP_YERPS)

Names and origin

Protein names

Recommended name:
    Periplasmic pectate lyase
    EC=4.2.2.2

Gene names

Name:	pelY
Ordered Locus Names:	YPTB3834

Organism

Yersinia pseudotuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconic acid from pectin: step 2/5.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the polysaccharide lyase 2 family.
Sequence caution	The sequence AAA27660.1 differs from that shown. Reason: Frameshift at position 539.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pectin catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 572	549	Periplasmic pectate lyase		PRO_0000024911

Sequences

	Sequence		Length	Mass (Da)
	P11278-1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: A01ADC208C2B5FB3	FASTA	572	64,115
	10 20 30 40 50 60 MKKRALLLSM SVLAMLYIPA GQAAEIDRLT VVKQYVDNVL NKASDTYHGD KPSPLLADGV 70 80 90 100 110 120 DPRTGQQMEW IFPDGRRAVL SNFSAQQNLM RVMSGLSELS GDPQYQKRAE DIVRYHFQNY 130 140 150 160 170 180 QDNSGLLYWG GHRFVDLKTL QPEGPSEKEK VHELKNAYPY YDLMFSVDSD ATTRFIRGFW 190 200 210 220 230 240 NAHVYDWRIL ETSRHGEYGK PMGALWESTF EQQPPFFATK GLSFLNAGND LIYSASLLYK 250 260 270 280 290 300 YQQDQGALVW AKRLADQYVL PRDAKTGLGV YQFTQALKRE EPTDDADTHS KFGDRAQRQF 310 320 330 340 350 360 GPEFGPTALE GNMMLKGRTS TLYSENALMQ LQLGKDLGGQ GDDLLKWTVD GLKAFAKYGY 370 380 390 400 410 420 NEQDNTFRPM IANGQDLSNY TLPRDGYYGK KGSVLKPYKA GNEFLISYAR AYAVDNDPLL 430 440 450 460 470 480 WKVARGIASD QGLGDIGSAP GKEMKVKLDT TNSDPYALFA LLDLYNASQV AEYRSLAEKV 490 500 510 520 530 540 ADNIIKTRYI DGFFMASPDR QYADVDAIEP YALLALEASL RNKPQAVAPF LNGAGFTEGA 550 560 570 YLMADGSARI STRDNELFLL NVGETLQPNG RK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequencing of a pectate lyase gene from Yersinia pseudotuberculosis." Manulis S., Kobayashi D.Y., Keen N.T. J. Bacteriol. 170:1825-1830(1988) [PubMed: 2832382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ICBP 3821.
[2]	"Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis." Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O., Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L., Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C., Simonet M., Chenal-Francisque V., Souza B. , Dacheux D., Elliott J.M., Derbise A., Hauser L.J., Garcia E. Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004) [PubMed: 15358858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IP32953 / Serotype I.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M19399 Genomic DNA. Translation: AAA27660.1. Frameshift. BX936398 Genomic DNA. Translation: CAH23072.1.
PIR	WZEPPY. A27742.
RefSeq	YP_072315.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL2. Polysaccharide Lyase Family 2.
Genome annotation databases
GeneID	2956229.
GenomeReviews	Gene locus YPTB3834 in contig BX936398_GR.
KEGG	yps:YPTB3834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P11278.
OMA	P11278. RPMLANG.
Enzyme and pathway databases
BioCyc	YPSE273123:YPTB3834-MON.
BRENDA	4.2.2.2. 20871.
Family and domain databases
InterPro	IPR010702. Pectate_lyase_2. [Graphical view]
Pfam	PF06917. Pectate_lyase_2. 1 hit. [Graphical view]
PIRSF	PIRSF001432. Pect_lyase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PELP_YERPS

Accession

Primary (citable) accession number: P11278
Secondary accession number(s): Q664D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)