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Protein

Calcium/calmodulin-dependent protein kinase type II subunit alpha

Gene

Camk2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (PubMed:15312654). Phosphorylates transcription factor FOXO3 on 'Ser-298'. Activates FOXO3 transcriptional activity (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATPPROSITE-ProRule annotation1
Active sitei135Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.17. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type II subunit alpha (EC:2.7.11.17By similarity)
Short name:
CaM kinase II subunit alpha
Short name:
CaMK-II subunit alpha
Gene namesi
Name:Camk2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2261. Camk2a.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cell junction Source: UniProtKB-KW
  • dendrite Source: UniProtKB
  • dendrite cytoplasm Source: BHF-UCL
  • mitochondrion Source: ParkinsonsUK-UCL
  • neuronal cell body Source: RGD
  • nucleus Source: RGD
  • postsynaptic density Source: RGD
  • presynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi432 – 435IRIT → MGTA: Loss of interaction with MPDZ. 1 Publication4

Chemistry databases

ChEMBLiCHEMBL2359.
GuidetoPHARMACOLOGYi1555.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860941 – 478Calcium/calmodulin-dependent protein kinase type II subunit alphaAdd BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphotyrosineBy similarity1
Modified residuei257PhosphoserineCombined sources1
Modified residuei286Phosphothreonine; by autocatalysis1 Publication1
Modified residuei330PhosphoserineBy similarity1
Modified residuei331PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Modified residuei336PhosphothreonineBy similarity1
Modified residuei337PhosphothreonineBy similarity1
Modified residuei404PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP11275.

PTM databases

iPTMnetiP11275.
PhosphoSitePlusiP11275.
SwissPalmiP11275.

Miscellaneous databases

PMAP-CutDBP11275.

Interactioni

Subunit structurei

CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Chrm3P084832EBI-2640645,EBI-7946407
Chrm4P0848510EBI-2640645,EBI-7946147
Grin2bQ009602EBI-2640645,EBI-396905
Lrrc7P705872EBI-2640645,EBI-7798464
PSMC5P621954EBI-2640645,EBI-357745From a different organism.

GO - Molecular functioni

  • glutamate receptor binding Source: ParkinsonsUK-UCL
  • GTPase activating protein binding Source: RGD

Protein-protein interaction databases

BioGridi247435. 13 interactors.
DIPiDIP-29639N.
IntActiP11275. 15 interactors.
MINTiMINT-153011.

Chemistry databases

BindingDBiP11275.

Structurei

Secondary structure

1478
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi295 – 309Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CDMX-ray2.00B290-314[»]
1CM1X-ray2.00B290-314[»]
1CM4X-ray2.00B290-314[»]
ProteinModelPortaliP11275.
SMRiP11275.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11275.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 271Protein kinasePROSITE-ProRule annotationAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni290 – 300Calmodulin-bindingAdd BLAST11
Regioni310 – 320Interaction with BAALC1 PublicationAdd BLAST11

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG108055.
InParanoidiP11275.
KOiK04515.
PhylomeDBiP11275.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR013543. Ca/CaM-dep_prot_kinase-assoc.
IPR011009. Kinase-like_dom.
IPR032710. NTF2-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF08332. CaMKII_AD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P11275-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS
60 70 80 90 100
ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED
110 120 130 140 150
IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA
160 170 180 190 200
AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG
210 220 230 240 250
VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK
260 270 280 290 300
MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK
310 320 330 340 350
GAILTTMLAT RNFSGGKSGG NKKNDGVKES SESTNTTIED EDTKVRKQEI
360 370 380 390 400
IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE
410 420 430 440 450
NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE
460 470
ETRVWHRRDG KWQIVHFHRS GAPSVLPH
Length:478
Mass (Da):54,115
Last modified:July 1, 1989 - v1
Checksum:i306F416CCE9B5F62
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti301G → A in AAA41855 (PubMed:3037704).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02942 mRNA. Translation: AAA41870.1.
M16960 mRNA. Translation: AAA41855.1.
M29699 Genomic DNA. Translation: AAA40841.1.
PIRiA30355.
RefSeqiNP_037052.1. NM_012920.1.
UniGeneiRn.107499.

Genome annotation databases

GeneIDi25400.
KEGGirno:25400.
UCSCiRGD:2261. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02942 mRNA. Translation: AAA41870.1.
M16960 mRNA. Translation: AAA41855.1.
M29699 Genomic DNA. Translation: AAA40841.1.
PIRiA30355.
RefSeqiNP_037052.1. NM_012920.1.
UniGeneiRn.107499.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CDMX-ray2.00B290-314[»]
1CM1X-ray2.00B290-314[»]
1CM4X-ray2.00B290-314[»]
ProteinModelPortaliP11275.
SMRiP11275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247435. 13 interactors.
DIPiDIP-29639N.
IntActiP11275. 15 interactors.
MINTiMINT-153011.

Chemistry databases

BindingDBiP11275.
ChEMBLiCHEMBL2359.
GuidetoPHARMACOLOGYi1555.

PTM databases

iPTMnetiP11275.
PhosphoSitePlusiP11275.
SwissPalmiP11275.

Proteomic databases

PRIDEiP11275.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25400.
KEGGirno:25400.
UCSCiRGD:2261. rat.

Organism-specific databases

CTDi815.
RGDi2261. Camk2a.

Phylogenomic databases

HOVERGENiHBG108055.
InParanoidiP11275.
KOiK04515.
PhylomeDBiP11275.

Enzyme and pathway databases

BRENDAi2.7.11.17. 5301.

Miscellaneous databases

EvolutionaryTraceiP11275.
PMAP-CutDBP11275.
PROiP11275.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR013543. Ca/CaM-dep_prot_kinase-assoc.
IPR011009. Kinase-like_dom.
IPR032710. NTF2-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF08332. CaMKII_AD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCC2A_RAT
AccessioniPrimary (citable) accession number: P11275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.