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P11275

- KCC2A_RAT

UniProt

P11275 - KCC2A_RAT

Protein

Calcium/calmodulin-dependent protein kinase type II subunit alpha

Gene

Camk2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei42 – 421ATPPROSITE-ProRule annotation
    Active sitei135 – 1351Proton acceptor

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin-dependent protein kinase activity Source: BHF-UCL
    3. GTPase activating protein binding Source: RGD
    4. protein binding Source: IntAct

    GO - Biological processi

    1. ionotropic glutamate receptor signaling pathway Source: UniProtKB
    2. peptidyl-serine phosphorylation Source: RGD
    3. protein autophosphorylation Source: RGD
    4. protein phosphorylation Source: BHF-UCL

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.17. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent protein kinase type II subunit alpha (EC:2.7.11.17)
    Short name:
    CaM kinase II subunit alpha
    Short name:
    CaMK-II subunit alpha
    Gene namesi
    Name:Camk2a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2261. Camk2a.

    Subcellular locationi

    Cell junctionsynapsepresynaptic cell membrane 1 Publication. Cell junctionsynapse 1 Publication
    Note: Postsynaptic lipid rafts.

    GO - Cellular componenti

    1. calcium- and calmodulin-dependent protein kinase complex Source: UniProtKB
    2. cell junction Source: UniProtKB-KW
    3. dendrite Source: UniProtKB
    4. dendrite cytoplasm Source: BHF-UCL
    5. presynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi432 – 4354IRIT → MGTA: Loss of interaction with MPDZ. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Calcium/calmodulin-dependent protein kinase type II subunit alphaPRO_0000086094Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131PhosphotyrosineBy similarity
    Modified residuei286 – 2861Phosphothreonine; by autocatalysis1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP11275.
    PRIDEiP11275.

    PTM databases

    PhosphoSiteiP11275.

    Miscellaneous databases

    PMAP-CutDBP11275.

    Expressioni

    Gene expression databases

    GenevestigatoriP11275.

    Interactioni

    Subunit structurei

    CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Chrm3P084832EBI-2640645,EBI-7946407
    Chrm4P0848510EBI-2640645,EBI-7946147
    Lrrc7P705872EBI-2640645,EBI-7798464
    PSMC5P621954EBI-2640645,EBI-357745From a different organism.

    Protein-protein interaction databases

    BioGridi247435. 13 interactions.
    DIPiDIP-29639N.
    IntActiP11275. 14 interactions.
    MINTiMINT-153011.
    STRINGi10116.ENSRNOP00000025283.

    Structurei

    Secondary structure

    1
    478
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi295 – 30915

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CDMX-ray2.00B290-314[»]
    1CM1X-ray2.00B290-314[»]
    1CM4X-ray2.00B290-314[»]
    ProteinModelPortaliP11275.
    SMRiP11275. Positions 7-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11275.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 271259Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni290 – 30011Calmodulin-bindingAdd
    BLAST
    Regioni310 – 32011Interaction with BAALCAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG108055.
    KOiK04515.
    PhylomeDBiP11275.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR013543. Ca/CaM-dep_prot_kinase-assoc.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF08332. CaMKII_AD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P11275-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS    50
    ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED 100
    IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA 150
    AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG 200
    VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK 250
    MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK 300
    GAILTTMLAT RNFSGGKSGG NKKNDGVKES SESTNTTIED EDTKVRKQEI 350
    IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE 400
    NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE 450
    ETRVWHRRDG KWQIVHFHRS GAPSVLPH 478
    Length:478
    Mass (Da):54,115
    Last modified:July 1, 1989 - v1
    Checksum:i306F416CCE9B5F62
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti301 – 3011G → A in AAA41855. (PubMed:3037704)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02942 mRNA. Translation: AAA41870.1.
    M16960 mRNA. Translation: AAA41855.1.
    M29699 Genomic DNA. Translation: AAA40841.1.
    PIRiA30355.
    RefSeqiNP_037052.1. NM_012920.1.
    UniGeneiRn.107499.

    Genome annotation databases

    GeneIDi25400.
    KEGGirno:25400.
    UCSCiRGD:2261. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02942 mRNA. Translation: AAA41870.1 .
    M16960 mRNA. Translation: AAA41855.1 .
    M29699 Genomic DNA. Translation: AAA40841.1 .
    PIRi A30355.
    RefSeqi NP_037052.1. NM_012920.1.
    UniGenei Rn.107499.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CDM X-ray 2.00 B 290-314 [» ]
    1CM1 X-ray 2.00 B 290-314 [» ]
    1CM4 X-ray 2.00 B 290-314 [» ]
    ProteinModelPortali P11275.
    SMRi P11275. Positions 7-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247435. 13 interactions.
    DIPi DIP-29639N.
    IntActi P11275. 14 interactions.
    MINTi MINT-153011.
    STRINGi 10116.ENSRNOP00000025283.

    Chemistry

    BindingDBi P11275.
    ChEMBLi CHEMBL2359.

    PTM databases

    PhosphoSitei P11275.

    Proteomic databases

    PaxDbi P11275.
    PRIDEi P11275.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25400.
    KEGGi rno:25400.
    UCSCi RGD:2261. rat.

    Organism-specific databases

    CTDi 815.
    RGDi 2261. Camk2a.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG108055.
    KOi K04515.
    PhylomeDBi P11275.

    Enzyme and pathway databases

    BRENDAi 2.7.11.17. 5301.

    Miscellaneous databases

    EvolutionaryTracei P11275.
    NextBioi 606489.
    PMAP-CutDB P11275.

    Gene expression databases

    Genevestigatori P11275.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR013543. Ca/CaM-dep_prot_kinase-assoc.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF08332. CaMKII_AD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase."
      Lin C.R., Kapiloff M.S., Durgerian S., Tatemoto K., Russo A.F., Hanson P., Schulman H., Rosenfeld M.G.
      Proc. Natl. Acad. Sci. U.S.A. 84:5962-5966(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Conserved and variable regions in the subunits of brain type II Ca2+/calmodulin-dependent protein kinase."
      Bulleit R.F., Bennett M.K., Molloy S.S., Hurley J.B., Kennedy M.B.
      Neuron 1:63-72(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Functional analysis of a complementary DNA for the 50-kilodalton subunit of calmodulin kinase II."
      Hanley R.M., Means A.R., Ono T., Kemp B.E., Burgin K.E., Waxham N., Kelly P.T.
      Science 237:293-297(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 132-327.
    4. "Sequence analysis and DNA-protein interactions within the 5' flanking region of the Ca2+/calmodulin-dependent protein kinase II alpha-subunit gene."
      Sunyer T., Sahyoun N.
      Proc. Natl. Acad. Sci. U.S.A. 87:278-282(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
    5. "Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I."
      Benfenati F., Valtorta F., Rubenstein J.L., Gorelick F.S., Greengard P., Czernik A.J.
      Nature 359:417-420(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 231-236; 238-246 AND 461-476, INTERACTION WITH SYN1.
    6. "Ca2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity."
      Thiel G., Czernik A.J., Gorelick F., Nairn A.C., Greengard P.
      Proc. Natl. Acad. Sci. U.S.A. 85:6337-6341(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 282-299, ENZYME REGULATION, PHOSPHORYLATION AT THR-286.
    7. "Characterization of a calmodulin kinase II inhibitor protein in brain."
      Chang B.H., Mukherji S., Soderling T.R.
      Proc. Natl. Acad. Sci. U.S.A. 95:10890-10895(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CAMK2N2.
    8. "SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation."
      Krapivinsky G., Medina I., Krapivinsky L., Gapon S., Clapham D.E.
      Neuron 43:563-574(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYNGAP1 AND MPDZ, MUTAGENESIS OF 432-ILE--THR-435, FUNCTION.
    9. "BAALC 1-6-8 protein is targeted to postsynaptic lipid rafts by its N-terminal miristoylation and palmitoylation, and interacts with a, but not b, subunit of Ca2+/calmodulin-dependent protein kinase II."
      Wang X., Tian Q.-B., Okano A., Sakagami H., Moon I.S., Kondo H., Endo S., Suzuki T.
      J. Neurochem. 92:647-659(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BAALC, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiKCC2A_RAT
    AccessioniPrimary (citable) accession number: P11275
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3