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Protein

Myeloperoxidase

Gene

Mpo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.1 Publication

Catalytic activityi

Cl- + H2O2 + H+ = HClO + H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per monomer.By similarity
  • heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234Heme (covalent; via 3 links)By similarity1
Active sitei235Proton acceptorPROSITE-ProRule annotation1
Metal bindingi236CalciumPROSITE-ProRule annotation1
Metal bindingi308CalciumPROSITE-ProRule annotation1
Metal bindingi310Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi312CalciumPROSITE-ProRule annotation1
Metal bindingi314CalciumPROSITE-ProRule annotation1
Sitei379Transition state stabilizerPROSITE-ProRule annotation1
Binding sitei382Heme (covalent; via 3 links)By similarity1
Binding sitei383Heme (covalent; via 3 links)By similarity1
Metal bindingi476Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • defense response to bacterium Source: GO_Central
  • defense response to fungus Source: MGI
  • hydrogen peroxide catabolic process Source: MGI
  • hypochlorous acid biosynthetic process Source: MGI
  • low-density lipoprotein particle remodeling Source: MGI
  • negative regulation of growth of symbiont in host Source: MGI
  • oxidation-reduction process Source: MGI
  • removal of superoxide radicals Source: MGI
  • respiratory burst involved in defense response Source: MGI
  • response to food Source: Ensembl
  • response to gold nanoparticle Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to mechanical stimulus Source: Ensembl
  • response to yeast Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei3344. MmMPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Myeloperoxidase (EC:1.11.2.21 Publication)
Short name:
MPO
Cleaved into the following 2 chains:
Gene namesi
Name:Mpo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97137. Mpo.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2440.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
PropeptideiPRO_000002365716 – 138Sequence analysisAdd BLAST123
ChainiPRO_0000023658139 – 718MyeloperoxidaseAdd BLAST580
ChainiPRO_0000023659139 – 252Myeloperoxidase light chainAdd BLAST114
ChainiPRO_0000023660253 – 718Myeloperoxidase heavy chainAdd BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi141 ↔ 154PROSITE-ProRule annotation
Disulfide bondi255 ↔ 265PROSITE-ProRule annotation
Disulfide bondi259 ↔ 283PROSITE-ProRule annotation
Modified residuei290Cysteine sulfenic acid (-SOH)By similarity1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi329N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi361 ↔ 372PROSITE-ProRule annotation
Glycosylationi365N-linked (GlcNAc...)Sequence analysis1
Glycosylationi457N-linked (GlcNAc...)Sequence analysis1
Modified residuei490Nitrated tyrosineBy similarity1
Disulfide bondi580 ↔ 637PROSITE-ProRule annotation
Disulfide bondi678 ↔ 704PROSITE-ProRule annotation
Glycosylationi711N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Nitration, Oxidation

Proteomic databases

MaxQBiP11247.
PaxDbiP11247.
PeptideAtlasiP11247.
PRIDEiP11247.

PTM databases

iPTMnetiP11247.
PhosphoSitePlusiP11247.

Expressioni

Gene expression databases

BgeeiENSMUSG00000009350.
CleanExiMM_MPO.
ExpressionAtlasiP11247. baseline and differential.
GenevisibleiP11247. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain (By similarity).By similarity

Protein-protein interaction databases

BioGridi201479. 1 interactor.
IntActiP11247. 1 interactor.
STRINGi10090.ENSMUSP00000020779.

Structurei

3D structure databases

ProteinModelPortaliP11247.
SMRiP11247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2408. Eukaryota.
ENOG410XPZ3. LUCA.
GeneTreeiENSGT00550000074325.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.
InParanoidiP11247.
KOiK10789.
OMAiKSSGCAY.
OrthoDBiEOG091G02JC.
TreeFamiTF314316.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR029609. MPO.
[Graphical view]
PANTHERiPTHR11475:SF49. PTHR11475:SF49. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLLALAGL LAPLAMLQTS NGATPALLGE VENSVVLSCM EEAKQLVDRA
60 70 80 90 100
YKERRESIKR SLQSGSASPT ELLFYFKQPV AGTRTAVRAA DYLHVALDLL
110 120 130 140 150
KRKLQPLWPR PFNVTDVLTP AQLNLLSVSS GCAYQDVRVT CPPNDKYRTI
160 170 180 190 200
TGHCNNRRSP TLGASNRAFV RWLPAEYEDG VSMPFGWTPG VNRNGFKVPL
210 220 230 240 250
ARQVSNAIVR FPNDQLTKDQ ERALMFMQWG QFLDHDITLT PEPATRFSFF
260 270 280 290 300
TGLNCETSCL QQPPCFPLKI PPNDPRIKNQ KDCIPFFRSC PACTRNNITI
310 320 330 340 350
RNQINALTSF VDASGVYGSE DPLARKLRNL TNQLGLLAIN TRFQDNGRAL
360 370 380 390 400
MPFDSLHDDP CLLTNRSARI PCFLAGDMRS SEMPELTSMH TLFVREHNRL
410 420 430 440 450
ATQLKRLNPR WNGEKLYQEA RKIVGAMVQI ITYRDYLPLV LGPAAMKKYL
460 470 480 490 500
PQYRSYNDSV DPRIANVFTN AFRYGHTLIQ PFMFRLNNQY RPTGPNPRVP
510 520 530 540 550
LSKVFFASWR VVLEGGIDPI LRGLMATPAK LNRQNQIVVD EIRERLFEQV
560 570 580 590 600
MRIGLDLPAL NMQRSRDHGL PGYNAWRRFC GLPQPSTVGE LGTVLKNLEL
610 620 630 640 650
ARKLMAQYGT PNNIDIWMGG VSEPLEPNGR VGQLLACLIG TQFRKLRDGD
660 670 680 690 700
RFWWENPGVF SKQQRQALAS ISLPRIICDN TGITTVSKNN IFMSNTYPRD
710
FVSCNTLPKL NLTSWKET
Length:718
Mass (Da):81,182
Last modified:July 27, 2011 - v2
Checksum:iE6763BE528E2ED83
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61S → T in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti61S → T in CAA33439 (PubMed:2552419).Curated1
Sequence conflicti138R → G in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti138R → G in CAA33439 (PubMed:2552419).Curated1
Sequence conflicti339I → V in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti339I → V in CAA33439 (PubMed:2552419).Curated1
Sequence conflicti494 – 495GP → AA in CAA33373 (PubMed:2548170).Curated2
Sequence conflicti494 – 495GP → AA in CAA33439 (PubMed:2552419).Curated2
Sequence conflicti676I → L in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti676I → L in CAA33439 (PubMed:2552419).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15313 mRNA. Translation: CAA33373.1.
X15378 Genomic DNA. Translation: CAA33439.1.
AL604022 Genomic DNA. Translation: CAI35961.1.
CCDSiCCDS25217.1.
PIRiS06068.
RefSeqiNP_034954.2. NM_010824.2.
UniGeneiMm.4668.

Genome annotation databases

EnsembliENSMUST00000020779; ENSMUSP00000020779; ENSMUSG00000009350.
ENSMUST00000121303; ENSMUSP00000112837; ENSMUSG00000009350.
GeneIDi17523.
KEGGimmu:17523.
UCSCiuc011ycc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15313 mRNA. Translation: CAA33373.1.
X15378 Genomic DNA. Translation: CAA33439.1.
AL604022 Genomic DNA. Translation: CAI35961.1.
CCDSiCCDS25217.1.
PIRiS06068.
RefSeqiNP_034954.2. NM_010824.2.
UniGeneiMm.4668.

3D structure databases

ProteinModelPortaliP11247.
SMRiP11247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201479. 1 interactor.
IntActiP11247. 1 interactor.
STRINGi10090.ENSMUSP00000020779.

Chemistry databases

ChEMBLiCHEMBL2440.

Protein family/group databases

PeroxiBasei3344. MmMPO.

PTM databases

iPTMnetiP11247.
PhosphoSitePlusiP11247.

Proteomic databases

MaxQBiP11247.
PaxDbiP11247.
PeptideAtlasiP11247.
PRIDEiP11247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020779; ENSMUSP00000020779; ENSMUSG00000009350.
ENSMUST00000121303; ENSMUSP00000112837; ENSMUSG00000009350.
GeneIDi17523.
KEGGimmu:17523.
UCSCiuc011ycc.1. mouse.

Organism-specific databases

CTDi4353.
MGIiMGI:97137. Mpo.

Phylogenomic databases

eggNOGiKOG2408. Eukaryota.
ENOG410XPZ3. LUCA.
GeneTreeiENSGT00550000074325.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.
InParanoidiP11247.
KOiK10789.
OMAiKSSGCAY.
OrthoDBiEOG091G02JC.
TreeFamiTF314316.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP11247.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000009350.
CleanExiMM_MPO.
ExpressionAtlasiP11247. baseline and differential.
GenevisibleiP11247. MM.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR029609. MPO.
[Graphical view]
PANTHERiPTHR11475:SF49. PTHR11475:SF49. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPERM_MOUSE
AccessioniPrimary (citable) accession number: P11247
Secondary accession number(s): Q5NCP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.