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Protein

Myeloperoxidase

Gene

Mpo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.1 Publication

Catalytic activityi

Cl- + H2O2 + H+ = HClO + H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per monomer.By similarity
  • heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234Heme (covalent; via 3 links)By similarity1
Active sitei235Proton acceptorPROSITE-ProRule annotation1
Metal bindingi236CalciumPROSITE-ProRule annotation1
Metal bindingi308CalciumPROSITE-ProRule annotation1
Metal bindingi310Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi312CalciumPROSITE-ProRule annotation1
Metal bindingi314CalciumPROSITE-ProRule annotation1
Sitei379Transition state stabilizerPROSITE-ProRule annotation1
Binding sitei382Heme (covalent; via 3 links)By similarity1
Binding sitei383Heme (covalent; via 3 links)By similarity1
Metal bindingi476Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • defense response to bacterium Source: GO_Central
  • defense response to fungus Source: MGI
  • hydrogen peroxide catabolic process Source: MGI
  • hypochlorous acid biosynthetic process Source: MGI
  • low-density lipoprotein particle remodeling Source: MGI
  • negative regulation of growth of symbiont in host Source: MGI
  • oxidation-reduction process Source: MGI
  • removal of superoxide radicals Source: MGI
  • respiratory burst involved in defense response Source: MGI
  • response to food Source: Ensembl
  • response to gold nanoparticle Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to mechanical stimulus Source: Ensembl
  • response to yeast Source: MGI

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Protein family/group databases

PeroxiBasei3344 MmMPO

Names & Taxonomyi

Protein namesi
Recommended name:
Myeloperoxidase (EC:1.11.2.21 Publication)
Short name:
MPO
Cleaved into the following 2 chains:
Gene namesi
Name:Mpo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97137 Mpo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2440

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
PropeptideiPRO_000002365716 – 138Sequence analysisAdd BLAST123
ChainiPRO_0000023658139 – 718MyeloperoxidaseAdd BLAST580
ChainiPRO_0000023659139 – 252Myeloperoxidase light chainAdd BLAST114
ChainiPRO_0000023660253 – 718Myeloperoxidase heavy chainAdd BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi141 ↔ 154PROSITE-ProRule annotation
Disulfide bondi255 ↔ 265PROSITE-ProRule annotation
Disulfide bondi259 ↔ 283PROSITE-ProRule annotation
Modified residuei290Cysteine sulfenic acid (-SOH)By similarity1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi329N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi361 ↔ 372PROSITE-ProRule annotation
Glycosylationi365N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi457N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi580 ↔ 637PROSITE-ProRule annotation
Disulfide bondi678 ↔ 704PROSITE-ProRule annotation
Glycosylationi711N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Oxidation

Proteomic databases

MaxQBiP11247
PaxDbiP11247
PeptideAtlasiP11247
PRIDEiP11247

PTM databases

CarbonylDBiP11247
iPTMnetiP11247
PhosphoSitePlusiP11247

Expressioni

Gene expression databases

BgeeiENSMUSG00000009350
CleanExiMM_MPO
ExpressionAtlasiP11247 baseline and differential
GenevisibleiP11247 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain (By similarity).By similarity

Protein-protein interaction databases

BioGridi201479, 2 interactors
IntActiP11247, 1 interactor
STRINGi10090.ENSMUSP00000020779

Structurei

3D structure databases

ProteinModelPortaliP11247
SMRiP11247
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2408 Eukaryota
ENOG410XPZ3 LUCA
GeneTreeiENSGT00550000074325
HOGENOMiHOG000016084
HOVERGENiHBG000071
InParanoidiP11247
KOiK10789
OMAiKVFFASW
OrthoDBiEOG091G02JC
TreeFamiTF314316

Family and domain databases

Gene3Di1.10.640.10, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR019791 Haem_peroxidase_animal
IPR037120 Haem_peroxidase_sf
IPR029609 MPO
PANTHERiPTHR11475:SF108 PTHR11475:SF108, 1 hit
PfamiView protein in Pfam
PF03098 An_peroxidase, 1 hit
PRINTSiPR00457 ANPEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS50292 PEROXIDASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLLALAGL LAPLAMLQTS NGATPALLGE VENSVVLSCM EEAKQLVDRA
60 70 80 90 100
YKERRESIKR SLQSGSASPT ELLFYFKQPV AGTRTAVRAA DYLHVALDLL
110 120 130 140 150
KRKLQPLWPR PFNVTDVLTP AQLNLLSVSS GCAYQDVRVT CPPNDKYRTI
160 170 180 190 200
TGHCNNRRSP TLGASNRAFV RWLPAEYEDG VSMPFGWTPG VNRNGFKVPL
210 220 230 240 250
ARQVSNAIVR FPNDQLTKDQ ERALMFMQWG QFLDHDITLT PEPATRFSFF
260 270 280 290 300
TGLNCETSCL QQPPCFPLKI PPNDPRIKNQ KDCIPFFRSC PACTRNNITI
310 320 330 340 350
RNQINALTSF VDASGVYGSE DPLARKLRNL TNQLGLLAIN TRFQDNGRAL
360 370 380 390 400
MPFDSLHDDP CLLTNRSARI PCFLAGDMRS SEMPELTSMH TLFVREHNRL
410 420 430 440 450
ATQLKRLNPR WNGEKLYQEA RKIVGAMVQI ITYRDYLPLV LGPAAMKKYL
460 470 480 490 500
PQYRSYNDSV DPRIANVFTN AFRYGHTLIQ PFMFRLNNQY RPTGPNPRVP
510 520 530 540 550
LSKVFFASWR VVLEGGIDPI LRGLMATPAK LNRQNQIVVD EIRERLFEQV
560 570 580 590 600
MRIGLDLPAL NMQRSRDHGL PGYNAWRRFC GLPQPSTVGE LGTVLKNLEL
610 620 630 640 650
ARKLMAQYGT PNNIDIWMGG VSEPLEPNGR VGQLLACLIG TQFRKLRDGD
660 670 680 690 700
RFWWENPGVF SKQQRQALAS ISLPRIICDN TGITTVSKNN IFMSNTYPRD
710
FVSCNTLPKL NLTSWKET
Length:718
Mass (Da):81,182
Last modified:July 27, 2011 - v2
Checksum:iE6763BE528E2ED83
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61S → T in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti61S → T in CAA33439 (PubMed:2552419).Curated1
Sequence conflicti138R → G in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti138R → G in CAA33439 (PubMed:2552419).Curated1
Sequence conflicti339I → V in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti339I → V in CAA33439 (PubMed:2552419).Curated1
Sequence conflicti494 – 495GP → AA in CAA33373 (PubMed:2548170).Curated2
Sequence conflicti494 – 495GP → AA in CAA33439 (PubMed:2552419).Curated2
Sequence conflicti676I → L in CAA33373 (PubMed:2548170).Curated1
Sequence conflicti676I → L in CAA33439 (PubMed:2552419).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15313 mRNA Translation: CAA33373.1
X15378 Genomic DNA Translation: CAA33439.1
AL604022 Genomic DNA Translation: CAI35961.1
CCDSiCCDS25217.1
PIRiS06068
RefSeqiNP_034954.2, NM_010824.2
UniGeneiMm.4668

Genome annotation databases

EnsembliENSMUST00000020779; ENSMUSP00000020779; ENSMUSG00000009350
ENSMUST00000121303; ENSMUSP00000112837; ENSMUSG00000009350
GeneIDi17523
KEGGimmu:17523
UCSCiuc011ycc.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPERM_MOUSE
AccessioniPrimary (citable) accession number: P11247
Secondary accession number(s): Q5NCP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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