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P11247

- PERM_MOUSE

UniProt

P11247 - PERM_MOUSE

Protein

Myeloperoxidase

Gene

Mpo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity By similarity.By similarity

    Catalytic activityi

    Cl- + H2O2 + H+ = HClO + H2O.
    Cl- + H2O2 = HOCl + 2 H2O.

    Cofactori

    Binds 1 calcium ion per monomer.By similarity
    Binds 1 heme B (iron-protoporphyrin IX) group covalently per monomer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei234 – 2341Heme (covalent; via 3 links)By similarity
    Active sitei235 – 2351Proton acceptorPROSITE-ProRule annotation
    Metal bindingi236 – 2361CalciumPROSITE-ProRule annotation
    Metal bindingi308 – 3081CalciumPROSITE-ProRule annotation
    Metal bindingi310 – 3101Calcium; via carbonyl oxygenPROSITE-ProRule annotation
    Metal bindingi312 – 3121CalciumPROSITE-ProRule annotation
    Metal bindingi314 – 3141CalciumPROSITE-ProRule annotation
    Sitei379 – 3791Transition state stabilizerPROSITE-ProRule annotation
    Binding sitei382 – 3821Heme (covalent; via 3 links)By similarity
    Binding sitei383 – 3831Heme (covalent; via 3 links)By similarity
    Metal bindingi476 – 4761Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. heparin binding Source: Ensembl
    3. metal ion binding Source: UniProtKB-KW
    4. peroxidase activity Source: MGI

    GO - Biological processi

    1. aging Source: Ensembl
    2. defense response to fungus Source: MGI
    3. hydrogen peroxide catabolic process Source: MGI
    4. hypochlorous acid biosynthetic process Source: MGI
    5. low-density lipoprotein particle remodeling Source: Ensembl
    6. negative regulation of growth of symbiont in host Source: MGI
    7. removal of superoxide radicals Source: MGI
    8. respiratory burst involved in defense response Source: MGI
    9. response to food Source: Ensembl
    10. response to lipopolysaccharide Source: Ensembl
    11. response to mechanical stimulus Source: Ensembl
    12. response to yeast Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Biological processi

    Hydrogen peroxide

    Keywords - Ligandi

    Calcium, Heme, Iron, Metal-binding

    Protein family/group databases

    PeroxiBasei3344. MmMPO.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myeloperoxidase (EC:1.11.2.2)
    Short name:
    MPO
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Mpo
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:97137. Mpo.

    Subcellular locationi

    GO - Cellular componenti

    1. azurophil granule Source: UniProtKB
    2. extracellular space Source: Ensembl
    3. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Add
    BLAST
    Propeptidei16 – 138123Sequence AnalysisPRO_0000023657Add
    BLAST
    Chaini139 – 718580MyeloperoxidasePRO_0000023658Add
    BLAST
    Chaini139 – 252114Myeloperoxidase light chainPRO_0000023659Add
    BLAST
    Chaini253 – 718466Myeloperoxidase heavy chainPRO_0000023660Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi141 ↔ 154PROSITE-ProRule annotation
    Disulfide bondi255 ↔ 265PROSITE-ProRule annotation
    Disulfide bondi259 ↔ 283PROSITE-ProRule annotation
    Modified residuei290 – 2901Cysteine sulfenic acid (-SOH)By similarity
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi361 ↔ 372PROSITE-ProRule annotation
    Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi580 ↔ 637PROSITE-ProRule annotation
    Disulfide bondi678 ↔ 704PROSITE-ProRule annotation
    Glycosylationi711 – 7111N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Oxidation

    Proteomic databases

    PaxDbiP11247.
    PRIDEiP11247.

    PTM databases

    PhosphoSiteiP11247.

    Expressioni

    Gene expression databases

    ArrayExpressiP11247.
    BgeeiP11247.
    CleanExiMM_MPO.
    GenevestigatoriP11247.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000103563.

    Structurei

    3D structure databases

    ProteinModelPortaliP11247.
    SMRiP11247. Positions 131-717.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family. XPO subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG262194.
    GeneTreeiENSGT00550000074325.
    HOGENOMiHOG000016084.
    HOVERGENiHBG000071.
    InParanoidiQ5NCP1.
    KOiK10789.
    OMAiQDKYRTI.
    OrthoDBiEOG7M0NQW.
    TreeFamiTF314316.

    Family and domain databases

    Gene3Di1.10.640.10. 2 hits.
    InterProiIPR010255. Haem_peroxidase.
    IPR019791. Haem_peroxidase_animal.
    IPR029609. MPO.
    [Graphical view]
    PANTHERiPTHR11475:SF46. PTHR11475:SF46. 1 hit.
    PfamiPF03098. An_peroxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00457. ANPEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 1 hit.
    PROSITEiPS00435. PEROXIDASE_1. 1 hit.
    PS50292. PEROXIDASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11247-1 [UniParc]FASTAAdd to Basket

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    MKLLLALAGL LAPLAMLQTS NGATPALLGE VENSVVLSCM EEAKQLVDRA    50
    YKERRESIKR SLQSGSASPT ELLFYFKQPV AGTRTAVRAA DYLHVALDLL 100
    KRKLQPLWPR PFNVTDVLTP AQLNLLSVSS GCAYQDVRVT CPPNDKYRTI 150
    TGHCNNRRSP TLGASNRAFV RWLPAEYEDG VSMPFGWTPG VNRNGFKVPL 200
    ARQVSNAIVR FPNDQLTKDQ ERALMFMQWG QFLDHDITLT PEPATRFSFF 250
    TGLNCETSCL QQPPCFPLKI PPNDPRIKNQ KDCIPFFRSC PACTRNNITI 300
    RNQINALTSF VDASGVYGSE DPLARKLRNL TNQLGLLAIN TRFQDNGRAL 350
    MPFDSLHDDP CLLTNRSARI PCFLAGDMRS SEMPELTSMH TLFVREHNRL 400
    ATQLKRLNPR WNGEKLYQEA RKIVGAMVQI ITYRDYLPLV LGPAAMKKYL 450
    PQYRSYNDSV DPRIANVFTN AFRYGHTLIQ PFMFRLNNQY RPTGPNPRVP 500
    LSKVFFASWR VVLEGGIDPI LRGLMATPAK LNRQNQIVVD EIRERLFEQV 550
    MRIGLDLPAL NMQRSRDHGL PGYNAWRRFC GLPQPSTVGE LGTVLKNLEL 600
    ARKLMAQYGT PNNIDIWMGG VSEPLEPNGR VGQLLACLIG TQFRKLRDGD 650
    RFWWENPGVF SKQQRQALAS ISLPRIICDN TGITTVSKNN IFMSNTYPRD 700
    FVSCNTLPKL NLTSWKET 718
    Length:718
    Mass (Da):81,182
    Last modified:July 27, 2011 - v2
    Checksum:iE6763BE528E2ED83
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611S → T in CAA33373. (PubMed:2548170)Curated
    Sequence conflicti61 – 611S → T in CAA33439. (PubMed:2552419)Curated
    Sequence conflicti138 – 1381R → G in CAA33373. (PubMed:2548170)Curated
    Sequence conflicti138 – 1381R → G in CAA33439. (PubMed:2552419)Curated
    Sequence conflicti339 – 3391I → V in CAA33373. (PubMed:2548170)Curated
    Sequence conflicti339 – 3391I → V in CAA33439. (PubMed:2552419)Curated
    Sequence conflicti494 – 4952GP → AA in CAA33373. (PubMed:2548170)Curated
    Sequence conflicti494 – 4952GP → AA in CAA33439. (PubMed:2552419)Curated
    Sequence conflicti676 – 6761I → L in CAA33373. (PubMed:2548170)Curated
    Sequence conflicti676 – 6761I → L in CAA33439. (PubMed:2552419)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15313 mRNA. Translation: CAA33373.1.
    X15378 Genomic DNA. Translation: CAA33439.1.
    AL604022 Genomic DNA. Translation: CAI35961.1.
    CCDSiCCDS25217.1.
    PIRiS06068.
    RefSeqiNP_034954.2. NM_010824.2.
    XP_006532467.1. XM_006532404.1.
    XP_006532468.1. XM_006532405.1.
    UniGeneiMm.4668.

    Genome annotation databases

    EnsembliENSMUST00000020779; ENSMUSP00000020779; ENSMUSG00000009350.
    ENSMUST00000121303; ENSMUSP00000112837; ENSMUSG00000009350.
    GeneIDi17523.
    KEGGimmu:17523.
    UCSCiuc011ycc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15313 mRNA. Translation: CAA33373.1 .
    X15378 Genomic DNA. Translation: CAA33439.1 .
    AL604022 Genomic DNA. Translation: CAI35961.1 .
    CCDSi CCDS25217.1.
    PIRi S06068.
    RefSeqi NP_034954.2. NM_010824.2.
    XP_006532467.1. XM_006532404.1.
    XP_006532468.1. XM_006532405.1.
    UniGenei Mm.4668.

    3D structure databases

    ProteinModelPortali P11247.
    SMRi P11247. Positions 131-717.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000103563.

    Chemistry

    ChEMBLi CHEMBL2440.

    Protein family/group databases

    PeroxiBasei 3344. MmMPO.

    PTM databases

    PhosphoSitei P11247.

    Proteomic databases

    PaxDbi P11247.
    PRIDEi P11247.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020779 ; ENSMUSP00000020779 ; ENSMUSG00000009350 .
    ENSMUST00000121303 ; ENSMUSP00000112837 ; ENSMUSG00000009350 .
    GeneIDi 17523.
    KEGGi mmu:17523.
    UCSCi uc011ycc.1. mouse.

    Organism-specific databases

    CTDi 4353.
    MGIi MGI:97137. Mpo.

    Phylogenomic databases

    eggNOGi NOG262194.
    GeneTreei ENSGT00550000074325.
    HOGENOMi HOG000016084.
    HOVERGENi HBG000071.
    InParanoidi Q5NCP1.
    KOi K10789.
    OMAi QDKYRTI.
    OrthoDBi EOG7M0NQW.
    TreeFami TF314316.

    Miscellaneous databases

    ChiTaRSi MPO. mouse.
    NextBioi 292132.
    PROi P11247.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P11247.
    Bgeei P11247.
    CleanExi MM_MPO.
    Genevestigatori P11247.

    Family and domain databases

    Gene3Di 1.10.640.10. 2 hits.
    InterProi IPR010255. Haem_peroxidase.
    IPR019791. Haem_peroxidase_animal.
    IPR029609. MPO.
    [Graphical view ]
    PANTHERi PTHR11475:SF46. PTHR11475:SF46. 1 hit.
    Pfami PF03098. An_peroxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00457. ANPEROXIDASE.
    SUPFAMi SSF48113. SSF48113. 1 hit.
    PROSITEi PS00435. PEROXIDASE_1. 1 hit.
    PS50292. PEROXIDASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiPERM_MOUSE
    AccessioniPrimary (citable) accession number: P11247
    Secondary accession number(s): Q5NCP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3