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Reviewed, UniProtKB/Swiss-Prot P11233 (RALA_HUMAN)

Last modified February 9, 2010. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ras-related protein Ral-A
Gene names
Name: RALA
Synonyms: RAL
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Subunit structure

Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Ref.9 Ref.10 Ref.11 Ref.13

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

C3P158791EBI-1036803,EBI-1042083From a different organism.
PPP2R1BP301541EBI-1036803,EBI-357094

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 203203Ras-related protein Ral-A
PRO_0000082693
Propeptide204 – 2063Removed in mature form By similarity
PRO_0000281344

Regions

Nucleotide binding24 – 296GTP
Nucleotide binding40 – 467GTP
Nucleotide binding127 – 1304GTP
Motif43 – 519Effector region By similarity

Amino acid modifications

Modified residue2031Cysteine methyl ester Probable
Lipidation2031S-geranylgeranyl cysteine Ref.8

Experimental info

Mutagenesis471K → E: Strongly reduces interaction with EXOC8. Ref.13
Mutagenesis471K → I: No effect on interaction with EXOC8. Ref.13
Mutagenesis481A → W: Strongly reduces interaction with EXOC8. Ref.13
Mutagenesis501S → W: Strongly reduces interaction with EXOC8. Ref.13
Mutagenesis521R → A: Strongly reduces interaction with EXOC8. Ref.13
Mutagenesis521R → W: No effect on interaction with EXOC8. Ref.13
Mutagenesis811N → A: No effect on interaction with EXOC8. Ref.13
Mutagenesis811N → R: Strongly reduces interaction with EXOC8. Ref.13
Sequence conflict1 – 22MA → MVDYL Ref.2
Sequence conflict1 – 22MA → MVDYL Ref.3

Secondary structure

1 ........................... 206
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P11233-1 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 6974341EA18C1975

FASTA20623,567
        10         20         30         40         50         60 
MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE 

        70         80         90        100        110        120 
EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV 

       130        140        150        160        170        180 
PFLLVGNKSD LEDKRQVSVE EAKNRAEQWN VNYVETSAKT RANVDKVFFD LMREIRARKM 

       190        200 
EDSKEKNGKK KRKSLAKRIR ERCCIL

« Hide

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References

« Hide 'large scale' references
[1]"Coding sequences of human ralA and ralB cDNAs."
Chardin P., Tavitian A.
Nucleic Acids Res. 17:4380-4380(1989) [PubMed: 2662142] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets."
Polakis P.G., Weber R.F., Nevins B., Didsbury J.R., Evans T., Snyderman R.
J. Biol. Chem. 264:16383-16389(1989) [PubMed: 2550440] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Platelet.
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[4]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed: 12690205] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed: 12853948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[8]"Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA."
Kinsella B.T., Erdman R.A., Maltese W.A.
J. Biol. Chem. 266:9786-9794(1991) [PubMed: 1903399] [Abstract]
Cited for: ISOPRENYLATION AT CYS-203.
[9]"Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity."
Jullien-Flores V., Dorseuil O., Romero F., Letourneur F., Saragosti S., Berger R., Tavitian A., Gacon G., Camonis J.H.
J. Biol. Chem. 270:22473-22477(1995) [PubMed: 7673236] [Abstract]
Cited for: INTERACTION WITH RALBP1.
[10]"Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral."
Rebhun J.F., Chen H., Quilliam L.A.
J. Biol. Chem. 275:13406-13410(2000) [PubMed: 10747847] [Abstract]
Cited for: INTERACTION WITH RALGPS1.
[11]"Ral GTPases regulate exocyst assembly through dual subunit interactions."
Moskalenko S., Tong C., Rosse C., Mirey G., Formstecher E., Daviet L., Camonis J., White M.A.
J. Biol. Chem. 278:51743-51748(2003) [PubMed: 14525976] [Abstract]
Cited for: INTERACTION WITH EXOC8.
[12]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[13]"Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase."
Jin R., Junutula J.R., Matern H.T., Ervin K.E., Scheller R.H., Brunger A.T.
EMBO J. 24:2064-2074(2005) [PubMed: 15920473] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 9-183 IN COMPLEX WITH EXOC8 AND GTP ANALOG, INTERACTION WITH EXOC2, MUTAGENESIS OF LYS-47; ALA-48; SER-50; ARG-52 AND ASN-81.
[14]"Crystal structure of the C3bot-RalA complex reveals a novel type of action of a bacterial exoenzyme."
Pautsch A., Vogelsgesang M., Traenkle J., Herrmann C., Aktories K.
EMBO J. 24:3670-3680(2005) [PubMed: 16177825] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 9-183 IN COMPLEX WITH GTP AND CLOSTRIDIUM EXOENZYME C3.
[15]"Molecular recognition of an ADP-ribosylating Clostridium botulinum C3 exoenzyme by RalA GTPase."
Holbourn K.P., Sutton J.M., Evans H.R., Shone C.C., Acharya K.R.
Proc. Natl. Acad. Sci. U.S.A. 102:5357-5362(2005) [PubMed: 15809419] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.66 ANGSTROMS) IN COMPLEX WITH GTP AND CLOSTRIDIUM EXOENZYME C3.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X15014 mRNA. Translation: CAA33118.1.
M29893 mRNA. Translation: AAA36542.1.
AF493910 mRNA. Translation: AAM12624.1.
AC004837 Genomic DNA. No translation available.
CH236951 Genomic DNA. Translation: EAL23994.1.
CH471073 Genomic DNA. Translation: EAW94123.1.
BC039858 mRNA. Translation: AAH39858.1.
IPIIPI00217519.
PIRTVHUAA. S04596.
RefSeqNP_005393.2.
UniGeneHs.6906

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UADX-ray2.10A/B9-183[»]
1ZC3X-ray2.00A/C9-183[»]
1ZC4X-ray2.50A/C9-183[»]
2A78X-ray1.81A9-183[»]
2A9KX-ray1.73A9-183[»]
2BOVX-ray2.66A1-206[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP11233. 3 interactions.
STRINGP11233.

PTM databases

PhosphoSiteP11233.

Proteomic databases

PRIDEP11233.

Genome annotation databases

EnsemblENST00000005257; ENSP00000005257; ENSG00000006451; Homo sapiens. [Genome view]
GeneID5898.
KEGGhsa:5898.
UCSCuc003thd.1. human.

Organism-specific databases

CTD5898.
GeneCardsGC07P039630.
H-InvDBHIX0006618.
HGNCHGNC:9839. RALA.
MIM179550. gene.
PharmGKBPA34197.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHBG745225.
HOVERGENP11233.
InParanoidP11233.
OMASAKRREN.
OrthoDBEOG99PDJK.
PhylomeDBP11233.

Enzyme and pathway databases

Pathway_Interaction_DBarf6_traffickingpathway. Arf6 trafficking events.
foxopathway. FoxO family signaling.
p38alphabetapathway. Regulation of p38-alpha and p38-beta.
ReactomeREACT_11061. Signalling by NGF.

Gene expression databases

ArrayExpressP11233.
BgeeP11233.
CleanExHS_RALA.
GenevestigatorP11233.
GermOnlineENSG00000006451. Homo sapiens.

Family and domain databases

InterProIPR003577. GTPase_Ras.
IPR013753. Ras.
IPR001806. Ras_GTPase.
IPR015591. Ras_Ral_related.
IPR020849. Ras_small_GTPase.
IPR005225. Small_GTP_bd.
[Graphical view]
PANTHERPTHR11708:SF124. Ras_Ral_related. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio22942.
SOURCESearch...

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Entry information

Entry nameRALA_HUMAN
AccessionPrimary (citable) accession number: P11233
Secondary accession number(s): A4D1W3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 9, 2010
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents