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Protein

Thioredoxin

Gene

Txn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (By similarity). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei26Deprotonates C-terminal active site CysBy similarity1
Active sitei32NucleophileBy similarity1
Sitei33Contributes to redox potential valueBy similarity1
Sitei34Contributes to redox potential valueBy similarity1
Active sitei35NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: RGD
  • cellular response to antibiotic Source: RGD
  • cellular response to drug Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to hyperoxia Source: RGD
  • cellular response to oxidative stress Source: GO_Central
  • glycerol ether metabolic process Source: InterPro
  • negative regulation of hydrogen peroxide-induced cell death Source: RGD
  • negative regulation of protein export from nucleus Source: RGD
  • negative regulation of transcription by RNA polymerase II Source: RGD
  • oxidation-reduction process Source: UniProtKB
  • positive regulation of DNA binding Source: UniProtKB
  • positive regulation of peptidyl-serine phosphorylation Source: RGD
  • regulation of protein import into nucleus, translocation Source: UniProtKB
  • response to activity Source: RGD
  • response to axon injury Source: RGD
  • response to dexamethasone Source: RGD
  • response to drug Source: RGD
  • response to radiation Source: UniProtKB
  • response to selenium ion Source: RGD
  • response to thyroxine Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processElectron transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin
Short name:
Trx
Gene namesi
Name:Txn
Synonyms:Txn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621157 Txn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001200112 – 105ThioredoxinAdd BLAST104

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei8N6-succinyllysineBy similarity1
Disulfide bondi32 ↔ 35Redox-activePROSITE-ProRule annotation
Modified residuei39N6-acetyllysineBy similarity1
Modified residuei62S-nitrosocysteineBy similarity1
Modified residuei69S-nitrosocysteineBy similarity1
Modified residuei73S-nitrosocysteine; alternateBy similarity1
Disulfide bondi73Interchain; alternateBy similarity
Modified residuei94N6-acetyllysine; alternateBy similarity1
Modified residuei94N6-succinyllysine; alternateBy similarity1

Post-translational modificationi

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins (By similarity).By similarity

Keywords - PTMi

Acetylation, Disulfide bond, S-nitrosylation

Proteomic databases

PaxDbiP11232
PRIDEiP11232

PTM databases

iPTMnetiP11232
PhosphoSitePlusiP11232
SwissPalmiP11232

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD

Protein-protein interaction databases

BioGridi250461, 1 interactor
IntActiP11232, 2 interactors
MINTiP11232
STRINGi10116.ENSRNOP00000016447

Structurei

3D structure databases

ProteinModelPortaliP11232
SMRiP11232
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 105ThioredoxinPROSITE-ProRule annotationAdd BLAST104

Sequence similaritiesi

Belongs to the thioredoxin family.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0907 Eukaryota
COG0526 LUCA
HOGENOMiHOG000292977
HOVERGENiHBG009243
InParanoidiP11232
KOiK03671
PhylomeDBiP11232
TreeFamiTF318932

Family and domain databases

InterProiView protein in InterPro
IPR005746 Thioredoxin
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PANTHERiPTHR10438 PTHR10438, 1 hit
PfamiView protein in Pfam
PF00085 Thioredoxin, 1 hit
PIRSFiPIRSF000077 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P11232-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLIESKEA FQEALAAAGD KLVVVDFSAT WCGPCKMIKP FFHSLCDKYS
60 70 80 90 100
NVVFLEVDVD DCQDVAADCE VKCMPTFQFY KKGQKVGEFS GANKEKLEAT

ITEFA
Length:105
Mass (Da):11,673
Last modified:January 23, 2007 - v2
Checksum:i3A39025935FD42DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14878 mRNA Translation: CAA33019.1
AF311055 mRNA Translation: AAG49923.1
BC058454 mRNA Translation: AAH58454.1
PIRiS04352
RefSeqiNP_446252.1, NM_053800.3
UniGeneiRn.29777

Genome annotation databases

GeneIDi116484
KEGGirno:116484
UCSCiRGD:621157 rat

Similar proteinsi

Entry informationi

Entry nameiTHIO_RAT
AccessioniPrimary (citable) accession number: P11232
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health